• Korean Journal of Plant Taxonomy
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• v.49 no.3
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• pp.224-239
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• 2019

Species identification for the Korean tribe Desmodieae was conducted using the DNA barcoding genes rbcL, matK (from chloroplast DNA) and ITS (from nuclear ribosomal DNA). A total of 25 taxa (n = 75) in five genera were sequenced, and neighbor-joining trees were constructed using different combinations of DNA barcodes. When comparing these phylogenetic trees, a tree with all loci combined (rbcL + matK + ITS) showed the highest rate of identification success (72%). On this tree, two subtribes and five genera within the tribe were supported as monophyletic. In the Desmodiinae clade, Desmodium and Hylodesmum were more closely related to each other than to Ohwia. In the Hylodesmum clade, H. oldhamii was found to be a sister to H. podocarpum complex, and all taxa within the complex were identified successfully. Subsp. fallax, regarded as a variety of subsp. oxyphyllum, is closely clustered with subsp. podocarpum. Although var. mandshuricum has been regarded as a synonym of var. oxyphyllum, this taxon is supported as a distinct variety. For the Lespedezinae clade, all species of Kummerowia were monophyletic, while nine of 16 Lespedeza taxa were identified successfully. In particular, the resolution of Macrolespedeza (28.5%) was lower than that of Junceae (77.8%). Among the Lespedeza taxa, L. cuneata was distinguishable from L. lichiyuniae, despite morphological similarities. It has been suggested that both L. maritima and L. inschanica are hybrids. The former is thought to be an independent species. While it is difficult to determine whether the latter originated via hybridization, this study showed that it is closely related to L. juncea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.4
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• pp.391-397
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• 2014

Anthocyanins are water-soluble plant pigments that give rise to the red, purple, or blue colors observed in many crops. Especially purple pigment of colored rice represent biological activities has been an increased interest, due to the functional meal as a staple food. These health benefits have been attributed to antioxidant property of anthocyanin. However, there have been little genetic source for development of new and various colored rice variety. A recently developed new variety, 'Jungmo1020', showed an unique characteristic of blue color of rice grain hull. We identified the pigment petunidin-3-O-glucoside (Pt-3-G) as a major compound with a value of $176.3{\pm}2.3mg/100g$ (68.3%) from 80% MeOH with 1% HCl extracts by UPLC/MS/MS. The content of Pt-3-G in the extracts using a solvent of 100% MeOH with 1% HCl was the highest with the values of $183.8{\pm}2.5mg/100g$. In addition, these extracts showed excellent antioxidatative activities by DPPH and ABTs assay.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.6
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• pp.1462-1470
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• 2015

The container BIC-code is a transport protocol for convenience in international shipping and combined transport environment. It is an identification code of a marine transport container which displays a wide variety of information including country's code. Recently, transportation through aircrafts and ships continues to rise. Thus fast and accurate processes are required in the ports to manage transportation. Accordingly, in this paper, we propose a BIC-code region extraction and recognition method using multiple thresholds. In the code recognition, applying a fixed threshold is not reasonable due to a variety of illumination conditions caused by change of weather, lightening, camera position, color of the container and so on. Thus, the proposed method selects the best recognition result at the final stage after applying multiple thresholds to recognition. For each threshold, we performs binarization, labeling, BIC-code pattern decision (horizontal or vertical pattern) by morphological close operation, and character separation from the BIC-code. Then, each characters is recognized by template matching. Finally we measure recognition confidence scores for all the thresholds and choose the best one. Experimental results show that the proposed method yields accurate recognition for the container BIC-code with robustness to illumination change.

• The Korean Journal of Quaternary Research
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• v.17 no.2
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• pp.85-99
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• 2003

Since the experience of major landscape change during last half of the century due to war and rapid urbanization, the traditional character has been weakened, and it is necessary for a reconsideration to improve the landscape for the future. To review these relationships, the importance of a comprehensive understanding of nature has been suggested. Therefore identification of a new concept based on the 'socio-cultural influence of landscape' and 'sense of place' which are related to peoples' previous experience, is required. Furthermore more practical definitions and criteria to reveal the relationship are necessary. Among the terms suggested to describe sense of place such as 'home', 'place identity', 'place-based meaning' and 'settlement identity' etc., the 'home' is selected to represent our surrounding landscape. For more practical classification of home landscape, additional terms are suggested and defined based on both the relationships between human beings and nature, and between memory derived from previous experience and shared values with in the community. The additional terms which are the most important in the role of landscape character related to humans' are; ⅰ) Personal Landscape: Landscape of an individual human, which derives from previous personal experience; involves distinguishable character for a given person, and it is emotional and flexible depending on circumstances. ⅱ) Ordinary Landscape： Landscape of the 'common interest' between members of a community, which is acceptable as a surrounding for everyday daily life, it produces the richness and variety of landscape. ⅲ) Kernel Landscape： Landscape of the 'common ground' which is acceptable to the majority members of the community, and it provides variety and stability for periods of time, and it could strongly represent community attitudes toward nature. ⅳ) Prototype Landscape: Landscape as the 'common denominator' of overall community from past to present and towards the future, which encompasses all the kernel landscape throughout history. It provides a sense of place, balances the homogeneity of character throughout overall communities. Some part of this can be shared throughout history to shape an overall sense of place. It can also represent short terms fashions. For a prototype landscape to reveal sense of place, there are a couple of points which we should underline the commencing point. Firstly, understanding the relationship between humans and nature should be based on a given character of surroundings. Secondly, reoccurring landscape elements which have sustained in history can lead to sense of place, and should be reviewed the influences between nature and humans.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.21-21
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• 2017

Severe lodging has recurrently occurred at strong typhoon's hitting in recent climate change. The identification of quantitative trait loci (QTLs) and their responsible genes associated with a strong culm and their pyramiding are important for developing high-yielding varieties with a superior lodging resistance. To identify QTLs for lodging resistance, the tropical japonica line, Chugoku 117 and the improved indica variety, Habataki were selected as the donor parent, as these had thick and strong culms compared with the temperate japonica varieties in Japan such as Koshihikari. By using chromosome segment substitution lines (CSSLs) in which chromosome segments from the japonica variety were replaced to them from Habataki, we identified the QTLs for strong culm on chrs. 1 and 6, which were designated as STRONG CULM1 (SCM1) and STRONG CULM2 (SCM2), respectively. By using recombinant inbred lines (BILs) derived from a cross between Chugoku 117 and Koshihikari and introgression lines, we also identified the other QTLs for strong culm on chrs. 3 and 2, which were designated as STRONG CULM3 (SCM3) and STRONG CULM4 (SCM4), respectively. Candidate region of SCM1 includes Gn1 related to grain number. SCM2 was identical to APO1, a gene related to the control of panicle branch number, and SCM3 was identical to FC1, a strigolactone signaling associated gene, by performing fine mapping and positional cloning of these genes. To evaluate the effects of SCM1~SCM4 on lodging resistance, the Koshihiakri near isogenic line (NIL) with the introgressed SCM1 or SCM2 locus of Habataki (NIL-SCM1, NIL-SCM2) and the another Koshihikari NIL with the introgeressed SCM3 or SCM4 locus of Chugoku 117 (NIL-SCM3, NIL-SCM4) were developed. Then, we developed the pyramiding lines with double or triple combinations derived from step-by-step crosses among NIL-SCM1 NIL-SCM4. Triple pyramiding lines (NIL-SCM1+2+3, ~ NIL-SCM1+3+4) showed the largest culm diameter and the highest culm strength among the combinations and increased spikelet number due to the pleiotropic effects of these genes. Pyramiding of strong culm genes resulted in much increased culm thickness, culm strength and spikelet number due to their additive effect. SCM1 mainly contributed to enhance their pyramiding effect. These results in this study suggest the importance of identifying the combinations of superior alleles of strong culm genes among natural variation and pyramiding these genes for improving high-yielding varieties with a superior lodging resistance.

• Horticultural Science & Technology
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• v.35 no.1
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• pp.98-107
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• 2017

A DNA profile database was constructed to investigate the genetic relatedness of 72 germplasm samples of Pyrus and related cultivars using microsatellite markers. Three P. pyrifolia, four P. commus, and one P. betulifolia cultivars with different morphological traits were screened using 387 pairs of microsatellite primers. A core set of 11 primer pairs was selected to obtain 133 polymorphic amplified fragments meeting three criteria: high polymorphism information contents (PIC), high repeatability, and distinct allele patterns. The number of alleles per locus ranged between 4 and 22. Average PIC was 0.743 (range: 0.557 - 0.879). Cluster analysis using the unweighted pair - group method with arithmetical average (UPGMA) separated the 72 pear cultivars and germplasm samples into four major groups: Chinese, European pears, and a cluster of 55 Asian pears that could be reclassify into two subcluster, I - $1^{st}$ and II - $2^{nd}$, according to pedigree information. Almost all of the cultivars were discriminated by 11 microsatellite marker genotypes. The microsatellite DNA profile database may be utilized as tool to verify distinctness, uniformity, and stability between candidate cultivar, and to verify in the distinctness of existing cultivars.

• KIPS Transactions on Computer and Communication Systems
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• v.5 no.4
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• pp.95-102
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• 2016

Computer security incident such as confidential information leak and data destruction are constantly growing and it becomes threat to information in digital devices. To respond against the incident, digital forensic techniques are also developing to help digital incident investigation. With the development of digital forensic technology, a variety of forensic artifact has been developed to trace the behavior of users. Also, a diversity of forensic tool has been developed to extract information from forensic artifact. However, there is a issue that information from forensic tools has its own forms. To solve this problem, it needs to process data when it is output from forensic tools. Then it needs to compare and analyze processed data to identify how data is related each other and interpret the implications. To reach this, it calls for effective method to store and output data in the course of data processing. This paper aims to propose DFIOC (Digital Forensic Indicators Of Compromise) that is capable of transcribing a variety of forensic artifact information effectively during incident analysis and response. DFIOC, which is XML based format, provides "Evidence" to represent various forensic artifacts in the incident investigation. Furthermore, It provides "Forensic Analysis" to report forensic analysis result and also gives "Indicator" to investigate the trace of incidence quickly. By logging data into one sheet in DFIOC format for forensic analysis process, it is capable of avoiding unnecessary data processing. Lastly, since collected information is recorded in a normalized format, data input and output becomes much easier as well as it will be convenient to use for identification of collected information and analysis of data relationship.

• Journal of Digital Convergence
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• v.10 no.10
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• pp.529-538
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• 2012

This study analyzed a variety of different kinds of usage patterns of four-digit personal identification numbers(PINs) based on the data collected from students attending at A-university in 2011. According to the analysis, the 4 digit PINs '1234' was being used most frequently which is similar to the findings of the study by the Daniel Amitay research. In addition, almost 50 percent of the students were using the four-digit PINs closely related to their personal information, and more than 10 percent of them were using it only by the reason of easiness to remember or convenience to use. Number '0' was the most frequently used, and girl students used it more than boys did. According to the result of analysis of four-digit PINs, it was found that number '1' was the most frequently used in the first and the third position. It was also found that number '2' and number '4' were the most frequently used in the second and the forth position respectively. Among students who had used number 3 in third position, around 63.6 percent of those students were using number 4 in the forth position in their PINs which showed highest frequency from analysis of double-digits connected together in each position. The analysis of PINs were represented by the polygonal (type) graph with that X-axis showed from first to forth position and Y-axis showed number had been used at each position. Among many polygonal graphs, the form showed an N-type which was called in type 1 took the first place by 35 percent.

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.113-113
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• 1998

In Saccharomyces cerevisiae UV irradiation and a variety of chemical DNA -damaging agents induce the transcription of specific genes, including several involved in DNA repair. One of the best characterized of DNA -damage inducible genes is PHRI, which encodes the apoenzyme for DNA photolyase. Basal-level and damage-induced expression of PHRI require an upstream activation sequence, UASPHRI. Here we report the identification of the UlvIE6 gene of S. cerevisiae as a regulator of UASPHRl activity. Surprisingly, the effect of deletion of UME6 is growth phase dependent. In wild-type cells PHRI is induced in late exponential phase, concomitant with the initiation of glycogen accumulation that precedes the diauxic shift. Deletion of UNIE6 abolishes this induction, decreases the steady-state concentration of photolyase molecules and PHRI mRNA, and increases the UV sensitivity of a rad2 mutant. The results suggest that UM E6 contributes to the regulated expression of a subset of damage-responsive genes in yeast. Furthermore, the upstream repression sequence, URSPHRI, is required for repression and damage-induced expression of PHRl. Here we show identification of YER169W and YDR096W as putative regulators acting through $URS_{PHRI}$. These open reading frames were designated as RPHI (YERl69W) and RPH2 (YDR096W) indicating regulator of PHRI. Simultaneous disruption of both genes showed a synergistic effect, producing a four-fold increase in basal level expression and a similar decrease m the induction ratio following treatment of methyl methanesulfonate(MMS). Mutation of the sequence ($AG_4$) bound by Rphlp rendered the promoter of PHRI insensitive to changes in RPHI or RPH2 status. The data suggest that RPHI and RPH2 act as damage-responsive negative regulators of PHRI. Surprisingly, the sequence bound by Rphlp in vitro is found to be $AG_4$ which is identical to the consensus binding site for the regulators Msn2p and Msn4p involved in stress-induced expression. Deletion of MSN2 and MSN4 has little effect on the induction$.$ ratio following DNA damage. However, all deletions led to a significant decrease in basal-level and induced expression of PHRI. These results imply that MSN2 and MSN4 are positive regulators of P HRI but are not required for DNA damage repression. [Supported by grant from NIH]om NIH]

• Journal of the Korean Society for Marine Environment & Energy
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• v.7 no.3
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• pp.122-130
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• 2004

Crude oil is complex mixture of thousands of different organic compound formed from a variety of organic materials that are chemically converted under differing geological conditions over long periods of time. Also oil composition varies according to crude source, refining, processing, handling and storage. The oil fingerprint method is application of specific knowledge of petrochemicals and use of sophisticated analytical equipment and techniques to identify the source(s) of oil pollution. KNMPA currently utilizes three primary analytical techniques: Gas Chromatography (GC), Fluorescence Spectroscopy(FL) and Infrared Spectroscopy(IR). Of all these techniques, GC technique are most widely used. Gas Chromatography is used as a primary analytical method because high reliableness, high separating efficiency and repeatability, but it is timeconsumable. The study results of identification of waterborne spilled oil by Fast Gas Chromatograph method showed that analytical time is cut down to 30minutes in comparison with packed column method and chromatograms represent high resolution and high repeatability.