• 제목/요약/키워드: value gap

검색결과 1,039건 처리시간 0.023초

Bacillus subtilis YG-95가 생산하는 protease의 정제와 특성 (Purification and Characterization of Protease Produced by Bacillus subtilis YG-95)

  • 변영각;김성호;주현규;이갑상;임무현
    • Applied Biological Chemistry
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    • 제41권5호
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    • pp.349-354
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    • 1998
  • Bacillus subtillis YG-95가 생산하는 protease를 ammonium sulfate$(35{\sim}85%)$ precipitation, DEAE-separose 6B, sephadex G-100을 통해 분리, 정제하였고 정제된 효소의 특성을 조사하였다. SDS-PAGE로 확인된 효소의 분자량은 약 43 kilodalton이었다. 효소반응의 최적 pH 및 최적온도는 각각 약 pH 10.0와 $55^{\circ}C$이었으며, 효소는 $pH\;5{\sim}12$ 까지 넓은 pH범위에서 안정성을 보였고, $45^{\circ}C$까지의 온도에서 안정하였다. 본 효소는 $Fe^{3+}$$Al^{3+}$에 의해서 활성이 저해되었으며, 저해제 중에서는 O-Phenanthroline, PMSF, SDS에 의해서 80%이상 저해를 받았고 SPI에 대한 기질 친화도는 $K_m$은 1.28 mg/mL이었다.

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아민첨가제를 사용하여 합성된 ZnO의 입자형상 및 광학적 특성 (Particle Shapes and Optical Property of Synthesized ZnO with Amine Additives)

  • 현혜현;현미호;이동규
    • 한국응용과학기술학회지
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    • 제33권1호
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    • pp.23-29
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    • 2016
  • 육방정계 우르자이츠형의 산화아연은 n형 반도체로써 3.37 eV의 넓은 밴드갭 에너지와 60 meV의 큰 엑시톤 바인딩 에너지를 가진 물질이다. 가스센서, 발광 다이오드, 염료 감응 태양 전지, 염료오염의 분해 등의 넓은 범위에서 활용이 가능하다. 합성 시 마이크로파 수열합성법을 사용하게 되면 높은 수율, 빠른 반응속도, 에너지 절약의 장점이 있다. 아민첨가제는 수산이온 생성 및 킬레이트 효과로 인해 산화아연 입자 형상을 조정하는 역할을 한다. 본 논문에서는 전구체로는 질산아연육수화물을 사용하였고, 형상조정제로는 에탄올아민, 에틸렌디아민, 디에틸렌트리아민, 헥사메틸렌테트라민을 사용하였다. 수산화소듐을 사용하여 용액을 pH 11로 조정하였다. 합성된 산화아연은 별모양, 막대형, 꽃모양, 원추형의 다양한 형상을 확인할 수 있었다. 아민첨가제에 의한 물리 화학적 특성과 광학적 특성을 분석하기 위해 XRD, SEM, EDS, FT-IR, UV-vis 스펙트럼, PL 스펙트럼을 사용하였다.

스마트 시티의 발전을 위한 빅데이터 플랫폼 구축과 적용 (Big Data Platform Construction and Application for Smart City Development)

  • 문승혁
    • 문화기술의 융합
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    • 제6권2호
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    • pp.529-534
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    • 2020
  • 인류 문명의 발전은 산업화에 따른 도시의 발전과 운송 기술의 진화와 맥을 같이한다. 지금까지의 도시 발전은 수송비 절감과 한정된 중심업무 지구로서의 토지 이용 요구에 따라 진행되어왔다. 도시 인구 밀집도의 지속적인 증가는 지가상승, 교통 혼잡, 빈부격차, 공해발생 등과 같이 사회·경제적으로 많은 문제를 일으켜 왔고 현재의 도시 생태 시스템으로는 전반적인 해결에 어려움이 있다. 그러나 문제의 해결을 위한 실마리는 바로 도시생태계 내에서 찾을 수 있다. 서울 심야버스 운행 노선의 설계는 바로 심야의 도시 내 이동 인구의 흐름을 정보통신 기술을 이용해 분석하여 얻은 결과이다. 이렇듯 도시 문제의 해결은 도시 생태계의 분석에서 출발하는 것이 타당하다. 문제의 원인을 알았다면 시민의 삶의 질을 높이기 위해 빅데이터 플랫폼 구축이 필요하고 이를 통한 해결이 가능하다. 이 플랫폼의 중심에는 정보통신 기술의 기반 하에 도시의 구성 요소와 연결되어 실시간 수집, 분석 및 적용되는 빅데이터가 자리 잡고 있어야한다. 본 연구를 통해 지속 가능한 스마트 시티를 위한 빅데이터 플랫폼 구축과 적용 방안에 대하여 고찰한다.

건축과 도시개발의 사회경제적 주요 영향과 효과에 관한 역사적 고찰 (A Study for the Historical Consideration of Social Economical Main Impacts and Spread In Architectural and Urban Development)

  • 이동헌
    • 건축역사연구
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    • 제14권4호
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    • pp.177-195
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    • 2005
  • Tough Korean architecture and urban environment has been developed for almost 100 years, it still remains various kinds of problems. Many attempts was carried out by every new plan to reduce the problems, it result in requiring more investment than before. It means all the investment for the development has been inefficient and immature to protect economic problems compared with the developed country. For the reason of economic problems it must be studied in historical cases which influenced the economic impacts, before the proposal of index with the economic theory. Searching typical architecture or urban development that brought about economic impacts can be classified into 3 cases. First case is the impact that caused the economic growth, increase, boom like the Westminster & others and the american architectures after the economic crisis. Second case is the impacts that was the origin of decline, shrink in economic as the Palace of Versailles, the skyscrape buildings in america before the economic crisis. Third case is the impact that was the both role of increase and decline in economic as the modern architecture in industrial revolution which led to the national economic growth and the gap between the rich and the poor, and as the american architecture that was the root of crisis and the revival in economic. From the case study, it is clear that architecture has relation with economics in various factors as mass production, labour, and another industries all over the history & the world. Now, architecture strongly needed not only to raise functional, cultural effect and value, but to predict and control the economic impacts with theory from further research of historical cases to policy and practise.

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The Use of Rasch Model in Developing a Short Form Based on Self-Reported Activity Measure for Low Back Pain

  • Choi, Bong-Sam
    • 한국전문물리치료학회지
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    • 제21권4호
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    • pp.56-66
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    • 2014
  • For maintaining adequate psychometric properties when reducing the number of items from an instrument, item level psychometrics is crucial. Strategies such as low item correlation or factor loadings, using classical test theory, have traditionally been advocated. The purpose of this study is to describe the development of a new short form assessing the impact of low back pain on physical activity. Rasch measurement model has been applied to the International Classification of Functioning, Disability and Health Activity Measure (ICF-AM). One hundred and one individuals with low back pain aged 19-89 years (mean age: $48.1{\pm}17.3$) who live in the community were participated in the study. Twenty-seven items of lifting/carrying construct of the ICF-AM were analyzed. Ten items were selected from the construct to create a short form. Item elimination criteria include: 1) high or low mean square (out of the range: .6-1.4 for the fit statistics), 2) similar item calibrations to adjacent items, 3) person separation value, and item-person map for potential gap in person ability continuum. All 10 items of the short form fit to the Rasch model except one item (i.e., carrying toddler on back). Despite its high infit and outfit statistics (1.90/2.17), the item had to be reinstated due to potential gaps at the upper extreme of person ability level. The short form had a slightly better spread of person ability continuum compared to the entire set of item. The created short form separated individuals with low back pain into nearly 4 groups, while the entire set of items separated the individuals into 6 groups. The findings prompted multidimensional models for better explanation of the lifting/carrying domain. The item level psychometrics based on the Rasch model can be useful in developing short forms with rationally retained items.

Realization of High Impedance Surface Characteristics Using a Periodically Transformed Artificial Magnetic Conductor Structure and Reduction Technique of Specific Absorption Rate

  • Lee, Seungwoo;Rhee, Seung-Yeop;Kim, Pan-Yeol;Kim, Nam
    • Journal of electromagnetic engineering and science
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    • 제13권2호
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    • pp.113-119
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    • 2013
  • We developed a transformed, symmetrical, mushroom-like surface without via holes in cells focused on a 2.4-GHz WLAN band. Each slot in the novel type structure plays a key role in modeling at the desired frequencies. The designed artificial magnetic conductor (AMC) has several advantages, including a small size, a wider bandwidth, a short reflecting distance to the antenna, and easy fabrication because there are no via holes. Overall dimensions of the AMC cell are 21 mm $(Width){\times}21mm$ $(Height){\times}2.6mm$ (Thickness), and the bandwidth is about three times wider (11.7%) compared to that of a conventional AMC (4.0%). For evaluating the performance of the proposed structure, a reflector, which periodically consists of the designed AMC cells, was developed. The antenna with the investigated AMC reflector not only works within a quarter of the wavelength because of the extremely high wave impedance generated by the AMC cells on the surface of the structure but also reduces the specific absorption rate (SAR). Electromagnetic field (EMF) exposure to a human phantom was analyzed by applying the designed reflector to the 2.4-GHz dipole antenna in a tablet PC. The calculated peak SAR averaged over 1 g was 0.125 W/kg when the input power was 1 W and the antenna was located at 20 cm from the human phantom. However, the SAR value was only 0.002 W/kg (i.e., 98.4% blocked) when the designed reflector was inserted in front of the antenna.

라미네이트 도재와 복합레진 시멘트의 결합강도에 관한 연구 (A STUDY ON THE VOND STRENGTH OF PORCELAIN LAMINATE AND COMPOSITE RESIN CEMENTS)

  • 김성일;임호남;박남수
    • 대한치과보철학회지
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    • 제29권1호
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    • pp.91-109
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    • 1991
  • The purpose of this study were to comfirm the effects of the thickness and kinds of porcelain, etchants, illumination time, elapsed time for the measurement, and chemical cure component to the bond strength of porcelain laminate and composite resin cement, and to compare the effects between the light cured resin and the dual cured resins. The etched porcelain surface, the sectioned surface crossing porcelain and resin after bonding, and the debonded surfaces were observed by the SEM. One product of laminate porcelain powder, one light cured resin and two dual cured resins were selected. Each resin cements are lightened through the thin porcelain disc which was cut from cylindrical porcelain specimen by the diamond saw, and by the light through the porcelain disc they were bonded. Changes of thickness and kinds of porcelain, etchants, illumination time, and the elapsed time for the measurement were considered as variables for the bond strength. And the bond strength of porcelain and dual cured resins under the conditions of autopolymerization or the removal of chemical cure component were measured and compared. Bond strength were measured by shear stress. The etched surface, the cross-sectioned surface, and the debonded surface of porcelain or resin were observed by SEM. On the summary of this study, the following conclusions can be stated; 1. Bond strength of light cured resin was decreased inversely by the thickened porcelain laminate and showed the lowest value to the masking dentin porcelain among 4 kinds of porcelain powder. 2. Bond strength of autopolymerization of dual cured resin without illumination in dark chamber were from 75% to 98% to the data of dual cured resin with illumination. 3. Bond strength of dual cured resin used without chemical cured components were same to them of light cured resin. 4. Cross-sectioned surface treated by silane did not show the gap between the porcelain and resin. 5. Illumination over 80 seconds did not make the significant increase of bond strength on all kinds of resin.

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사파이어 기판에 펄스 레이저 증착법으로 성장된 AlN 박막의 특성 (Characterization of AlN Thin Films Grown by Pulsed Laser Deposition on Sapphire Substrate)

  • 정은희;정준기;정래영;김성진;박상엽
    • 한국세라믹학회지
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    • 제50권6호
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    • pp.551-556
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    • 2013
  • AlN films with c-axis orientation and thermal conductivity characteristics were deposited by using Pulsed Laser Deposition and the films were characterized by changing the deposition conditions. In particular, we investigated the optimal conditions for the application of a heat sinking plane AlN thin film. Epitaxial AlN films were deposited on sapphire ($c-Al_2O_3$) single crystals by pulsed laser deposition (PLD) with an AlN target. AlN films were deposited at a fixed pressure of $2{\times}10^{-5}$ Torr, while the substrate temperature was varied from 500 to $700^{\circ}C$. According to the experimental results of the growth temperature of the thin film, AlN thin films were confirmed with a highly c-axis orientation, maximum grain size, and high thermal conductivity at $650^{\circ}C$. The thermal conductivity of the AlN thin film was found to increase compared to bulk AlN near the band gap value of 6.2 eV.

밸러스트 수 처리를 위한 Plasma Gun의 오존생성에 미치는 운전변수의 영향 (Effects of Operating Parameters on Ozone Production by Plasma Gun for Ballast Water Treatment)

  • 이현돈;김종오;정재우
    • 한국항해항만학회지
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    • 제34권3호
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    • pp.205-211
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    • 2010
  • 밸러스트수 처리를 위한 Plasma Gun의 전기적 특성과 오존 생성에 미치는 주요 운전변수들의 영향에 관해 실험실 규모 연구를 수행하였다. 방전개시전압보다 높은 전압이 인가되면 방전이 시작되고 오존이 생성되었으며 전압이 증가함에 따라 기체로 전달되는 에너지 및 오존 농도가 거의 선형적으로 증가하였다. 오존 생성 측면에서 최적의 에너지 효율성을 얻을 수 있는 전극간 거리가 존재하였으며 실험된 장치에서는 1.95 mm의 전극간 거리에서 최적 효율이 얻어졌다. 전기에너지 특성에 미치는 내부전극 재질의 영향은 무시할만한 수준이었으나 내부전극의 전기전도도와 열전도도 차이가 오존 생성에 큰 영향을 미치는 것으로 나타났다. 일정한 Plasma Gun 구조에서 오존 생성은 기체로 전달되는 에너지밀도에 의해 중요한 영향을 받는 것으로 나타났으며 유입되는 기체의 산소함량이 증가할수록 오존 생성이 증가하는 것으로 나타났다.

"상한론(傷寒論)"에 수록된 탕제(湯劑)의 복약법(服藥法)에 관한 연구 (A Study on the Methods of Taking Prescriptions in SANGHANRON)

  • 김영균;조수인
    • 한국한의학연구원논문집
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    • 제7권1호
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    • pp.39-54
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    • 2001
  • This is a study on the methods of taking prescriptions in SANGHANRON (傷寒論), and after this, numbers of results have been obtained. SANGHANRON was written by Chang-Ki (張機) in the 2nd century, so it reflexes the usage of prescriptions of previous age indirectly. And gave affects on the methods of taking prescriptions to the oriental medicine doctors of next generation. Before Han-Dynasty (漢代), there were not so many publications connected with Oriental Medicine. Besides, some books couldn't hand down to next generation due to the gap of time and space. As time goes by, letters in medical books changed little by little, so contents connected with taking decoctions changed too. The effects of decoction and herb tea can be changed by the flexibility of methods of taking medicines, so we have to decide what kinds of taking methods should be taken and adapted to patients by the most effective way. There are many kind of methods of taking decocted prescriptions in SANGHANRON, so Chang-Ki selected the most appropriate method considering degree and position of disease and condition of patient. But nowadays, due to inconvenient procedure of taking medicines, some methos are not in common in clinical medicin. So this study was started to look back upon the changes of taking prescriptions and gave effort to find out the propriety of variation of taking prescriptions. In SANGHANRON, many kinds of taking prescriptions appeared from once a day to six times a day, except these, some prescriptions have to be taken little by little. These methods of taking prescriptions simplified as time pass by, but this change may give influence to the effect of medicine and finally we can't gain expected therapeutic value. So we have to distinguish the methods -though complicated and troublesome- in clinical medicine to make perfection more perfect in treating patients, and further studies have to be followed to prove the propriety of these methods.

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