• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.33-46
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• 2006

Purpose : This study was conducted to investigate effects of 7 herbs among medicinals resolving dampness with aroma on vaginal microorganisms. Methods : Staphylococcus aureus, Methicillin- resistant Staphylococcus aureus, Candida albicans and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for vaginal flora. Medicinals resolving dampness with aroma, Pogostemonis Herba, Amomi Cardamomi Fructus, Amomi Semen, Atractylodis Rhizoma, Ammomi Tsao-ko Fructus, Alpiniae Katsumadaii Semen and Magnoliae Cortex were used in this study. In vitro antimicrobial activities were observed by optical density and colony test. Results : The optical density showed that Alpiniae Katsumadaii Semen and Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effects on pathogenic vaginal microorganisms (Methicillin-resistant Staphylococcus aureus). Pogostemonis Herba, Ammomi Tsao-ko Fructus had antimicrobial effects on Gardnerella vaginalis. The colony test showed that Alpiniae Katsumadaii Semen, Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effects on Methicillin-resistant Staphylococcus aureus. Pogostemonis Herbs, Alpiniae Katsumadaii Semen had antimicrobial effects aganist Gardnerella vaginalis. The optical density showed that Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effect on normal vaginal microorganisms (Streptococcus spp.). The colony test showed that medicinals resolving dampness with aroma had no antimicrobial effect on normal vaginal microorganisms. Conclusion : From this study, we could suggest that Pogostemonis Herba, Ammomi Tsao-ko Fructus, Alpiniae Katsumadaii Semen, Magnoliae Cortex of medicinals resolving dampness with aroma are available to antimicrobial agent of pathogenic vaginal microorganisms in vitro.

• Animal Bioscience
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• v.35 no.11
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• pp.1808-1816
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• 2022

Objective: The study of Hanwoo (Korean native cattle) has mainly been focused on meat quality and productivity. Recently the field of microbiome research has increased dramatically. However, the information on the microbiome in Hanwoo is still insufficient, especially relationship between vagina and feces. Therefore, the purpose of this study is to examine the microbial community characteristics by analyzing the 16S rRNA sequencing data of Hanwoo vagina and feces, as well as to confirm the difference and correlation between vaginal and fecal microorganisms. As a result, the goal is to investigate if fecal microbiome can be used to predict vaginal microbiome. Methods: A total of 31 clinically healthy Hanwoo that delivered healthy calves more than once in Cheongju, South Korea were enrolled in this study. During the breeding season, we collected vaginal and fecal samples and sequenced the microbial 16S rRNA genes V3-V4 hypervariable regions from microbial DNA of samples. Results: The results revealed that the phylum-level microorganisms with the largest relative distribution were Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria in the vagina, and Firmicutes, Bacteroidetes, and Spirochaetes in the feces, respectively. In the analysis of alpha, beta diversity, and effect size measurements (LefSe), the results showed significant differences between the vaginal and fecal samples. We also identified the function of these differentially abundant microorganisms by functional annotation analyses. But there is no significant correlation between vaginal and fecal microbiome. Conclusion: There is a significant difference between vaginal and fecal microbiome, but no significant correlation. Therefore, it is difficult to interrelate vaginal microbiome as fecal microbiome in Hanwoo. In a further study, it will be necessary to identify the genetic relationship of the entire microorganism between vagina and feces through the whole metagenome sequencing analysis and meta-transcriptome analysis to figure out their relationship.

• Parasites, Hosts and Diseases
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• v.58 no.5
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• pp.571-576
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• 2020

Extra-enteric infections by Blastocystis spp. have rarely been documented. Here, we report a case of extra-enteric blastocystosis in a patient with minimal cervicitis symptoms. A 47-year-old Hispanic female patient was attended in a primary health centre in Michoacan state, Mexico, for her routine gynaecological medical examination. As only symptom, she referred to a slight vaginal itching. The presence of several vacuolar-stages of Blastocystis spp. were identified by Papanicolaou staining; molecular identification was attempted by culture-PCR sequencing of a region of 18S gene from cervical and faecal samples obtained 2 months after cytological examination, even when patient declared that she tried self-medicating with vaginal ovules. Blastocystis ST1 was identified only in the faecal sample. The presence of Blastocystis spp. in the cervix of a patient with scarce symptomatology, demonstrates the extraordinary flexibility of this microorganism to adapt to new environments and niches.

• Development and Reproduction
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• v.11 no.3
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• pp.245-251
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• 2007

Vinclozolin(VCZ), a systemic dicarboximide fungicide, has been used in the control of diseases caused by microorganism of some species in fruits, vegatables and ornamental plants. Although VCZ itself is a very weak antagonist for androgen receptor binding, both melabolites M1 and M2 are effective antagonists. The present study was undertaken to examine whether prepubertal exposure to VCZ affects on the onset of puberty and the associated reproductive parameters such as hormone receptor expressions in female rats. VCZ(10 mg/kg/day) was administered daily from postnatal day 21(PND 21) through the day when the first vaginal opening(V.O.) was observed. Gross anatomy and weight of reproductive tissues were compared to test the VCZ's effects on the cell proliferation. Furthermore, histological studies were performed to assess the structural alterations in the tissues. To determine the transcriptional changes in progesterone receptor(PR), total RNAs were extracted and applied to the semi-quantitative reverse transcription polymerase chain reaction(RT-PCR). As a result, delayed V.O. was shown in the VCZ group(PND $34.00{\pm}1.22$) compared to the control group(PND $38.20{\pm}1.92$; p<0.01). VCZ treatment significantly decreased the wet weight of ovaries and uteri compared to the control group(p<0.01). Graafian follicles and corpora lutea were observed only in the ovaries from the control animals, while numerous primary, secondary follicles and small atretic follicles were observed in the ovaries from VCZ group. Similarly, hypotrophy of luminal and glandular uterine epithelium was found in the VCZ group. In the semi-quantitative RT-PCR studies, the transcriptional activity of PR in ovary(p<0.01) from VCZ group were significantly lower than those from the control group while in uterus were similar compared with the control group. The present studies demonstrated that the acute exposure to VCZ during the critical period of prepubertal stage could inactivate the reproductive system resulting delayed puberty in female rats.