• 한국축산식품학회지
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• 제43권3호
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• pp.441-453
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• 2023

The objective of this study was to investigate the physicochemical and microbiological characteristics of round of Hanwoo by vacuum packaging film materials, polyvinylidene chloride (PVDC) and ethylene vinyl alcohol (EVOH). The packaged beef samples were stored in refrigerated conditions (2±1℃) for 12 weeks. Physicochemical analysis with pH, surface color, thiobarbituric acid reactive substances (TBARS) values, and volatile basic nitrogen (VBN) values and microbiological analysis with aerobic plate count (APC) and metagenomic analysis of packaged beef samples were performed. The pH and surface color did not change substantially during the 12 weeks and EVOH-packaged beef tended to be lower than those of PVDC-packaged beef. PVDC- and EVOH-packaged samples showed low TBARS and VBN values below standard limits. APC did not exceed 7 Log CFU/g for both samples during storage. In metagenomic analysis, Firmicutes and Lactobacillaceae were dominant phylum and family of the PVDC- and EVOH-packaged beef. In both packaged samples, Dellaglioa algida was the dominant species during storage, with the notable difference being the presence of Lactococcus piscium. Therefore, this study provided the information on the quality of vacuum-packaged beef according to different vacuum films for long-term refrigerated storage.

• 한국축산식품학회지
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• 제18권3호
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• pp.216-223
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• 1998

This study was conducted to investigate the effects of the tenderness for the vacuum chilled, the air-frozen or the rechilled Holstein beef loin. The vacuum packaged beef was stored at 1$^{\circ}C$ and the air-packaged beef was stored at -20$^{\circ}C$ for 60 days. The rechilled beef was restored for 3 days at 1$^{\circ}C$ by using the vacuum chilled or the air-frozen beef. Hardness and chewiness of the vacuum chilled beef were better than those of the air-frozen beef. Hardness and chewiness were improved significantly(p＜0.05) after the 40 days storage for the vacuum chilled beef, but there was no significant improvement for the air-frozen beef during the 60 days storage. By the rechilling process, tenderness and myofibrillar fragmentation index of the vacuum chilled and air-frozen beef improved. Especially those were improved significantly(p＜0.05) in the vacuum chilled beef after the 20 days storage, and in the air-frozen beef after the 40 and 60 days storage.

• 한국축산식품학회지
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• 제25권2호
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• pp.127-133
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• 2005

진공 포장한 우육을 $0\pm1^{\circ}C$에서 8주간 냉장하면서 숙성 효과를 조사하였다. 우육의 경도, 저작성 및 전단력은 냉장 5주까지 저하되어 연화 효과가 있었으나 6주 후에는 그 효과가 없었다. 근원섬유단백질의 추출성, Mg-ATPase활성 및 근원섬유 소편화도는 6주까지 높아졌으나 그 후 8주까지 변화하지 않았다. 우육의 색도는 냉장 8주까지 양호하였다. 우육의 기호도는 5주까지 향상되었으나 6주 이후에는 오히려 저하되었다. 진공포장하여 냉장한 우육의 숙성 효과는 5주까지 기대할 수 있었다.

• 한국식품과학회지
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• 제20권1호
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• pp.85-89
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• 1988

본 실험은 포장우육에서 가장 주요한 부패균의 하나인 B. thermosphacta의 생육특성을 조사하여 포장육 실험의 기초 자료를 마련하고자 실시하였으며 진공포장우육에서 B. thermosphacta와 lactic acid bacteria의 존재에 따른 B. thermosphacta의 생육 특성을 조사하였다. 통기성 포장우육에서는 높은 우육의 pH 범위에서 pH가 B. thermosphacta의 생육에 크게 영향을 미치지 않았으며 진공포장우육에서는 pH 5.5-5.6에서 pH 5.7이상에서 보다 크게 증식이 지연되었다. 포장방법에 따른 B. thermosphacta의 증식은 pH 5.7-6.0의 우육에서 100%가스포장했을 때 이들의 증식이 중지되었고, $20%CO_2$와 $80%N_2$ 혼합가스포장 했을 때 가벼운 증식억제 효과를 보였다. 또한 1 actic acid bacteria의 B. thermosphacta 증식억제 작용은 통기성 포장에서 약하게 나타났으나, 진공포장에서는 pH가 낮을수록 크게 나타났으며, pH 5.5-5.6의 우육에서 B. thermosphacta 는 거의 성장이 중지되었다.

• 한국식품영양과학회지
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• 제25권6호
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• pp.944-950
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• 1996

본 연구는 외국산 냉장쇠고기의 국내 수입에 따른 한우쇠고기의 경쟁력 제고와 저장성 증진을 위하여 한우 암소 1등급의 등심을 재료로 포장방법에 따른 냉장저장 중 이화학적 성질 변화를 조사하였다. 시료는 진공포장(포장재: polyamide/polyethylene, $50ml/\m^2.24h.$ atm)과 일반포장(포장재: polyvinylchloride, $80~320ml/\m^2.24h.atm)을$ 하였으며, 포장된 각군은 다시 형광등 조사군과 암실 보존군으로 나누었다. pH 변화는 저장 21일 동안 진공포장군의 경우 형광등 조사 여부에 관계없이 점차 감소하는 경향이었으나, 일반포장군은 점차 상승하는 경향을 보였다. VBN값은 진공포장군과 일반포장군 모두 저장기간에 따라 증가하는 경향을 나타내었으며, 진공포장군이 일반포장군에 비하여 저장 15일째 이후부터 유의하게 낮았다. TBA 값은 저장 초기에 비하여 저장 전기간 동안 진공포장군이 일반포장군에 비하여 낮게 유지되었다. 적색도에서는 진공포장군과 일반포장군 모두 저장기간에 따라 점차 감소하는 경향이었으며, 진공포장군은 일반포장군에 비하여 전저장기간 동안 높은 값을 나타내었다. 지방산 조성변화를 보면, 저장기간에 따라 포화지방산의 함량은 점차 증가하는 반면, 불포화지방산의 함량은 감소하였는데, 그 변화정도는 진공포장군이 일반포장군에 비하여 크게 낮았다. 본 연구에서 형광등 조사군과 암실 보존군간에는 이화학적 성질의 차이가 뚜렷하지 않았다.

• 한국축산식품학회지
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• 제42권6호
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• pp.953-967
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• 2022

The objective of this study was to determine effects of aging methods (wet-aged, dry-aged, and packaged dry-aged) during 60 d on quality traits and microbial characteristics of beef. Wet-aged beef was packed by vacuum packaging and stored in a 4℃ refrigerator. Dry-aged beef was used without packaging. Packaged dry-aged beef was packaged in commercial bags. Dry-aged and packaged dry-aged samples were stored in a meat ager at 2℃-4℃ with 85%-90% relative humidity. Meat color, crust thickness, aging loss, cooking loss, Warner-Bratzler shear force (WBSF), texture profile analysis, Torrymeter, meat pH, water activity, volatile basic nitrogen (VBN), thiobarbituric acid reactant substances (TBARS), and microbial analysis were measured or performed every 15 d until 60 d of aging time. Meat color changed significantly with increasing aging time. Differences in meat color among aging methods were observed. Aging losses of dry-aged and packaged dry-aged samples were higher than those of wet-aged samples. Wet-aged beef showed higher cooking loss, but lower WBSF than dry-aged and packaged dry-aged beef. VBN and TBARS showed an increasing tendency with increasing aging time. Differences of VBN and TBARS among aging methods were found. Regarding microbial analysis, counts of yeasts and molds were different among aging methods at the initial aging time. Packaged dry-aged and dry-aged beef showed similar values or tendency. Significant changes occurred during aging in all aging methods. Packaged dry aging and dry aging could result in similar quality traits and microbial characteristics of beef.

• Asian-Australasian Journal of Animal Sciences
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• 제31권4호
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• pp.585-594
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• 2018

Objective: This study was to determine the bacterial diversity and monitor the community dynamic changes during storage of vacuum-packaged sliced raw beef as affected by Lactobacillus sakei and Lactobacillus curvatus. Methods: L. sakei and L. curvatus were separately incubated in vacuumed-packaged raw beef as bio-protective cultures to inhibit the naturally contaminating microbial load. Dynamic changes of the microbial diversity of inoculated or non-inoculated (control) samples were monitored at $4^{\circ}C$ for 0 to 38 days, using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Results: The DGGE profiles of DNA directly extracted from non-inoculated control samples highlighted the order of appearance of spoilage bacteria during storage, showing that Enterbacteriaceae and Pseudomonas fragi emerged early, then Brochothrix thermosphacta shared the dominant position, and finally, Pseudomonas putida showed up became predominant. Compared with control, the inoculation of either L. sakei or L. curvatus significantly lowered the complexity of microbial diversity and inhibited the growth of spoilage bacteria (p<0.05). Interestingly, we also found that the dominant position of L. curvatus was replaced by indigenous L. sakei after 13 d for L. curvatus-inoculated samples. Plate counts on selective agars further showed that inoculation with L. sakei or L. curvatus obviously reduced the viable counts of Enterbacteraceae, Pseudomonas spp. and B. thermosphacta during later storage (p<0.05), with L. sakei exerting greater inhibitory effect. Inoculation with both bio-protective cultures also significantly decreased the total volatile basic nitrogen values of stored samples (p<0.05). Conclusion: Taken together, the results proved the benefits of inoculation with lactic acid bacteria especially L. sakei as a potential way to inhibit growth of spoilage-related bacteria and improve the shelf life of vacuum-packaged raw beef.

• 한국축산식품학회지
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• 제38권2호
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• pp.338-349
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• 2018

The objective of this study was to investigate comparison of physicochemical, microbiological, and sensory characteristics of Hanwoo eye of round by various packaging methods [wrapped packaging (WP), modified atmosphere packaging (MAP), vacuum packaging (VP) with three different vacuum films, and vacuum skin packaging (VSP)] at a small scale. Packaged Hanwoo beef samples were stored in refrigerated conditions ($4{\pm}1^{\circ}C$) for 28 days. Packaged beef was sampled on days 0, 7, 14, 21, and 28. Physicochemical [pH, surface color, thiobarbituric acid reactive substances (TBARS), and volatile basic nitrogen (VBN) values], microbiological, and sensory analysis of packaged beef samples were performed. VP and VSP samples showed low TBARS and VBN values, and pH and surface color did not change substantially during the 28-day period. For VSP, total viable bacteria, psychrotrophic bacteria, lactic acid bacteria, and coliform counts were lower than those for other packaging systems. Salmonella spp. and Escherichia coli O157:H7 were not detected in any packaged beef samples. A sensory analysis showed that the scores for appearance, flavor, color, and overall acceptability did not change significantly until day 7. In total, VSP was effective with respect to significantly higher $a^*$ values, physicochemical stability, and microbial safety in Hanwoo packaging (p<0.05).

• 한국축산식품학회지
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• 제39권2호
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• pp.266-275
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• 2019

Although the production of dry-aged beef has been increasing, most purveyors are unaware of the changes in quality that ensue after completion of the aging period and do not adhere to specific guidelines for its packaging and storage. The objective of this study was to investigate the storage stability of vacuum-packaged dry-aged beef based on changes in microbial, physicochemical, and sensory properties during refrigeration at $4^{\circ}C$ for 21 d. The total aerobic bacterial count exceeded 6 Log CFU/g at approximately day 11 and significantly increased after day 14. Freshness indicators such as pH and volatile basic nitrogen content were acceptable until day 14 and 21, respectively. Based on the evaluation of overall sensory acceptability, the dry-aged beef was acceptable until 14 d without any sensory deterioration. Therefore, vacuum-packaged dry-aged beef could be stored for 11 d at $4^{\circ}C$ without any adverse effect on its microbial and sensory quality.

• 한국축산식품학회지
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• 제33권1호
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• pp.58-66
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• 2013

In this study, the effect of packaging methods [aerobic packaging (AP), vacuum packaging (VP) and modified atmosphere packaging (MAP: 75% $N_2$, 25% $CO_2$)] on colour, lipid quality and microbial growth of beef patties enhanced with flaxseed flour was investigated during storage at $2{\pm}1^{\circ}C$ for 10 d. L and a values of beef patties packaged in MAP and VP were higher (p<0.05) than that of the samples packaged in AP. Packaging in MAP and VP retarded the lipid oxidation (TBA value) and inhibited the bacterial growth of beef patties enhanced with flaxseed flour. Furthermore, TBA values in beef patties were correlated with a values (r = -0.340; p<0.05). Packaging in MAP was more effective than packaging in VP for inhibiting microbial growth. The samples packaged in VP lost their shape due to the compression by external atmosphere. Packaging treatment had no significant effect on saturated fatty acid (SFA), monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) levels in beef patties. The a and b, TBA and MUFA values changed during storage time. TBA values for beef patties increased during storage time, but did not reach to the limit value (1 mg/kg) until the end of the storage time. The results suggest that the shelf life of beef patties enhanced with flaxseed flour can be extended by packaging in MAP.