• Asian-Australasian Journal of Animal Sciences
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• v.29 no.1
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• pp.126-133
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• 2016

A gene from Actinomyces sp. Korean native goat (KNG) 40 that encodes an endo-${\beta}$-1,4-glucanase, EG1, was cloned and expressed in Escherichia coli (E. coli) $DH5{\alpha}$. Recombinant plasmid DNA from a positive clone with a 3.2 kb insert hydrolyzing carboxyl methyl-cellulose (CMC) was designated as pDS3. The entire nucleotide sequence was determined, and an open-reading frame (ORF) was deduced. The ORF encodes a polypeptide of 684 amino acids. The recombinant EG1 produced in E. coli $DH5{\alpha}$ harboring pDS3 was purified in one step using affinity chromatography on crystalline cellulose and characterized. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis/zymogram analysis of the purified enzyme revealed two protein bands of 57.1 and 54.1 kDa. The amino terminal sequences of these two bands matched those of the deduced ones, starting from residue 166 and 208, respectively. Putative signal sequences, a Shine.Dalgarno-type ribosomal binding site, and promoter sequences related to the consensus sequences were deduced. EG1 has a typical tripartite structure of cellulase, a catalytic domain, a serine-rich linker region, and a cellulose-binding domain. The optimal temperature for the activity of the purified enzyme was $55^{\circ}C$, but it retained over 90% of maximum activity in a broad temperature range ($40^{\circ}C$ to $60^{\circ}C$). The optimal pH for the enzyme activity was 6.0. Kinetic parameters, $K_m$ and $V_{max}$ of rEG1 were 0.39% CMC and 143 U/mg, respectively.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.518-524
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• 2010

A mannanase gene (man26B) was obtained from a sea bacterium, Paenibacillus sp. BME-14, through the constructed genomic library and inverse PCR. The gene of man26B had an open reading frame of 1,428 bp that encoded a peptide of 475- amino acid residues with a calculated molecular mass of 53 kDa. Man26B possessed two domains, a carbohydrate binding module (CBM) belonging to family 6 and a family 26 catalytic domain (CD) of glycosyl hydrolases, which showed the highest homology to Cel44C of P. polymyxa (60% identity). The optimum pH and temperature for enzymatic activity of Man26B were 4.5 and $60^{\circ}C$, respectively. The activity of Man26B was not affected by $Mg^{2+}$ and $Co^{2+}$, but was inhibited by $Hg^{2+},\;Ca^{2+},\;Cu^{2+},\;Mn^{2+},\;K^+,\;Na^+$, and $\beta$-mercaptoethanol, and slightly enhanced by $Pb^{2+}$ and $Zn^{2+}$. EDTA did not affect the activity of Man26B, which indicates that it does not require divalent ions to function. Man26B showed a high specific activity for LBG and konjac glucomannan, with $K_m,\;V_{max}$, and $k_{cat}$ values of 3.80 mg/ml, 91.70 ${\mu}mol$/min/mg protein, and 77.08/s, respectively, being observed when LBG was the substrate. Furthermore, deletion of the CBM6 domain increased the enzyme stability while enabling it to retain 80% and 60% of its initial activity after treatment at $80^{\circ}C$ and $90^{\circ}C$ for 30 min, respectively. This finding will be useful in industrial applications of Man26B, because of the harsh circumstances associated with such processes.

• Korean Journal of Poultry Science
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• v.43 no.4
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• pp.263-272
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• 2016

In this study, we aimed to identify the nonsynonymous single nucleotide polymorphisms (nsSNPs) located in lipid metabolism-related genes because lipids are an important factor affecting the taste and flavor of meat, and they predict the functional consequences. The results showed that we identified 139 common nsSNPs in all five Korean native chicken (KNC) lines from the 81 genes related to lipid metabolism. Furthermore, sorting intolerant from tolerant (SIFT) and polymorphism phenotyping v2 (Polyphen-2) analyses predicted that among the genes, 14 nsSNPs of nine genes might be deleterious. Protein domain prediction of the nine genes revealed that all deleterious nsSNPs identified in this study were located outside the functional domain. This observation suggests that the common deleterious nsSNPs might be dispensable and have a minor effect on the traits of the KNCs.

• The Korean Journal of Physiology and Pharmacology
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• v.12 no.5
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• pp.245-251
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• 2008

Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain $K^+$ channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like $K^+$ channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of TASK-3-like $K^+$ channel was $33.0{\pm}0.1$ pS at - 60 mV, and TASK-3 activity fell by $65{\pm}5%$ when the extracellular pH was changed from 7.3 to 6.3, indicating that the DRG $K^+$ channel is similar to cloned TASK-3 channel. TASK-3 mRNA and protein levels in brain, spinal cord, and DRG were significantly higher in injured animals than in sham-operated ones. These results indicate that TASK-3 channels are expressed and functional in DRG neurons and the expression level is up-regulated following SCI, and suggest that TASK-3 channel could act as a potential background $K^+$ channel under SCI-induced acidic condition.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.348-353
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• 2009

Organic materials reveal the remarkable absorption and high buffer capacity for nutrient. Hence, organic materials need some different nutrient management skill from inorganic one. The objective of this study was to evaluate the effect of EC level of nutrient solution on the yield and quality of cut rose grown in the mixed substrate of coir and perlite. 3 EC levels of nutrient solution was treated, which were 0.7, 1.0, 1.3 times of standard solution (Aichiken solution, Japan) for cut rose hydroponics. EC of the standard solution was changed by season following as 1.4 (Apr.~June), 1.0 (July~Aug.), 1.4 (Sep.~Oct.), and $1.6dS{\cdot}m^{-1}$ (Nov.~Mar.) subsequently. The supply of nutrient solution was controlled by the signal of water potential at -5kPa using frequency domain reflectometry (FDR) sensor. As the results, marketable yield was similar for all treatments until 3rd harvest, but was decreased in high EC level from 4th harvest to 7th harvest as final. 0.7 times of standard solution decreased the ratio of unmarketable rose having short stem below 70cm and increased the ratio of high quality rose having long stem above 91cm. The flower weight and stem diameter of cut rose was higher in the low EC treatment than the others.

• Journal of the Korean earth science society
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• v.25 no.6
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• pp.443-473
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• 2004

In northern Gohung granitic gneiss, porphyroblastic gneiss and migmatitic gneiss are widely distributed. Gneisses were plotted in granodiorite domain on an lUGS silica-alkali diagram. The amounts of trace elements (Li, Zn, Sc, Sr, Ni, V Y etc.) vs. $SiO_2$, somewhat decreased. Plagioclase showed a wide compositional range ($An_{32-48}$). $X_{alm}$ and $X_{sps}$ were higher in garnet rim and $X_{pyp}$ in garnet core. The rocks in the study area were formed from S and I-type magmas which generated from syn-collision and the late to post-orogenic tectonic environment. Metamorphic P-T conditions u·ere low to medium pressure, high temperature (803-913$^{\circ}C$, 6.1-7.3 kb) and overprinted by retrograde metamorphism (570-726$^{\circ}C$, 2.2-5.1 kb) and chloritization.

• Economic and Environmental Geology
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• v.31 no.1
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• pp.21-29
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• 1998

Broadband receiver functions are developed from teleseismic P waveforms recorded at Wonju (KSRS), Inchon (IRIS), and Pohang (PHN), and are analyzed to examine the crustal structure beneath the three stations. The teleseismic receiver functions are inverted in the time domain to the vertical P wave velocity structure beneath the stations. Clear P-to-S converted phases from the Moho interface are observed in teleseismic seismograms recorded at the three stations. We estimated the crustal velocity structures beneath the stations using the receiver function inversion. The general features of inversion results are as follows: (1) For Pohang station, there is a high velocity gradient at a 4~5 km deep for SE and NW back azimuth and a low velocity zone at around 10 km deep. The Moho depth is 28 km for NW direction. (2) The shallow crustal structure beneath Wonju station is somewhat complex and there is a high-velocity zone ($V_p{\simeq}6.8km/sec$) at 3 to 4 km deep. The average crustal thickness is 33 km, and a transition zone exists at a 30~33 km deep of lower crust, of which velocity is abruptly changed 6.4 to 7.9 km/sec. (3) For Inchon station, the crustal velocity gradient monotonously increases up to the Moho discontinuity and the velocity is abruptly changed from 6.2 km/sec to 7.9 km/sec at 29 km deep.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.22 no.2
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• pp.128-138
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• 2009

Objectives : The purpose of this study is to make a survey on the oriental medical care and to enlarge the domain of oriental medical treatment concerning facial paralysis. Methods : From May 2008 to April 2009, a clinical study was done on 100 inpatients who were diagnosed and treated as facial nerve paralysis at Mok-dong Oriental Medicine Hospital, Dong-shin University Results : The result were as the following 1. The distribution of sex: male 35%, female 65%. The distribution of age was disclosed that fifty was the most in 22 cases(22%). 2. The distribution of the region of facial palsy: Lt 58%(male 23%, female 35%) Rt 41%(male 11%, female 30%) 3. In distribution of contributing frequence in month, June was the most in number(12%) and in season, spring, summer, winter were the same percentage(26%). 4. In distribution of cause, stress(34%), excessive labor(25%), unknown(22%) were investigated higher than other conditions. 5. The distribution of past history: hypertension 14%, diabetes mellitus 4%, both(hypertension, diabetes mellitus) 6% 6. The distribution of period before admission: within a week 80%, 1$\sim$2 week 8%, 3$\sim$4 week 6% in order. 7. The distribution of attended symptoms: mastoid pain 43 cases, dryness of eye 26 cases, tearing 22 cases, headache 12 cases, dizziness 9 cases in order. 8. Other offices patients visited before coming to this clinic: oriental clinic or hospital 35%, local clinic or hospital 29%, none 26%, both(oriental clinic & local clinic) 10%. 9. The distribution of House-Brackmann grade on admission: Gr III 38%, Gr IV 26%, Gr II 22%, Gr V 14% in order. 10. The distribution of House-Brackmann grade on discharge: Gr II 34%, Gr I 32%, Gr III 22%, Gr V 7%, Gr IV 5% in order.

• Journal of Broadcast Engineering
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• v.17 no.5
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• pp.756-764
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• 2012

In current HEVC, prediction pixels are finally calculated by linear-function interpolation on two reference pixels. It is hard to expect good performance on the case of occurring large difference between two reference pixels. This paper decides more accurate prediction pixel values than current HEVC using linear function. While existing prediction process only uses two reference pixels, proposed method uses DCT-IF. DCT-IF analyses frequency characteristics of more than two reference pixels in frequency domain. And proposed method calculates prediction value adaptively by using linear-function, DCT-IF and cubic-function to decide more accurate interpolation value than to only use linear function. Cubic-function has a steep slope than linear-function. So, using cubic-function is utilized on edge in prediction unit. The complexity of encoder and decoder in HM6.0 has increased 3% and 1%, respectively. BD-rate has decreased 0.4% in luma signal Y, 0.3% in chroma signal U and 0.3% in chroma signal V in average. Through this experiment, proposed adaptive intra prediction method using DCT-IF and cubic-function shows increased performance than HM6.0.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.141-141
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• 2017

The ubiquitin-proteasome pathway is the major regulatory mechanism in a number of cellular processes for selective degradation of proteins and involves three steps: (1) ATP dependent activation of ubiquitin by E1 enzyme, (2) transfer of activated ubiquitin to E2 and (3) transfer of ubiquitin to the protein to be degraded by E3 complex. F-box proteins are subunit of SCF complex and involved in specificity for a target substrate to be degraded. F-box proteins regulate many important biological processes such as embryogenesis, floral development, plant growth and development, biotic and abiotic stress, hormonal responses and senescence. However, little is known about the F-box genes in wheat. The draft genome sequence of wheat (IWGSC Reference Sequence v1.0 assembly) used to analysis a genome-wide survey of the F-box gene family in wheat. The Hidden Markov Model (HMM) profiles of F-box (PF00646), F-box-like (PF12937), F-box-like 2 (PF13013), FBA (PF04300), FBA_1 (PF07734), FBA_2 (PF07735), FBA_3 (PF08268) and FBD (PF08387) domains were downloaded from Pfam database were searched against IWGSC Reference Sequence v1.0 assembly. RNA-seq paired-end libraries from different stages of wheat, such as stages of seedling, tillering, booting, day after flowering (DAF) 1, DAF 10, DAF 20, and DAF 30 were conducted and sequenced by Illumina HiSeq2000 for expression analysis of F-box protein genes. Basic analysis including Hisat, HTseq, DEseq, gene ontology analysis and KEGG mapping were conducted for differentially expressed gene analysis and their annotation mappings of DEGs from various stages. About 950 F-box domain proteins identified by Pfam were mapped to wheat reference genome sequence by blastX (e-value < 0.05). Among them, more than 140 putative F-box protein genes were selected by fold changes cut-offs of > 2, significance p-value < 0.01, and FDR<0.01. Expression profiling of selected F-box protein genes were shown by heatmap analysis, and average linkage and squared Euclidean distance of putative 144 F-box protein genes by expression patterns were calculated for clustering analysis. This work may provide valuable and basic information for further investigation of protein degradation mechanism by ubiquitin proteasome system using F-box proteins during wheat development stages.