• 농업과학연구
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• 제41권3호
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• pp.193-203
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• 2014

Natural Monument is a designated cultural property as part of the country. According to Article 2 of the Cultural Properties Protection Act, a national, ethnic and global heritage artificially or naturally formed, with a great historical, artistic, scientific and landscape significance is defined as a cultural heritage. Animals, plants, topography, geology, minerals, caves, biological products and special natural phenomena, having a great of historic, scenic and scientific value, are defined as the monument. According to Article 3 of Cultural Properties Protection Act, the conservation, management and utilization of National Heritage should be kept intact in its original form. So, Natural monuments are managing as retained its original form under the Basic Principles of current law. The highest population of coniferous tree in natural monument plant is ginkgo tree including 22 objects, followed by pines, junipers that order. And in case of broadleaf tree, there are zelkova trees, retusa fringe trees, pagoda trees, cork oaks, silver magnolias and etc. There are many of reported efficacy in available natural monument plants. The efficacy of plant species on pharmaceutical like anti-cancer, anti-diabetic, anti-obesity, antioxidant activity, neuroprotective, improves cholesterol, anti-inflammatory, liver protection and anti-bacterial efficacy, on cosmetics and beauty like the inhibiting formation of skin wrinkles, whitening effect, variety of materials and the efficacy of the proposed utilization of its various papers and etc have been widely reported. Before the Nagoya Protocol enters into force, the future role of the National Research Institute for Cultural Properties Administration of Cultural Heritage should be obtain a legal right to manage the social, cultural and national natural monument with emotional value to the plant genetic resource as a natural monument efficient ways to study and preserve traditional knowledge biological resources by securing a claim to the sovereignty of the material will be ready.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.482-493
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• 2005

Biomass is originally photosynthesized from inorgainic compounds such as $CO_2$, minerals, water and solar energy. Recent studies have shown that anaerobic bacteria have the ability to convert recalcitrant biomass such as cellullosic or chitinoic materials to useful compounds. The biomass containing agricultural waste, unutilized wood and other garbage is expected to utilize as feed, food and fuel by microbial degradation and other metabolic functions. In this study we isolated several anaerobic, cellulolytic and chitinolytic bacteria from rumen fluid, compost and soil to study their related enzymes and genes. The anaerobic and cellulolytic bacteria, Clostridium thermocellum, Clostridium stercorarium, and Clostridium josui, were isolated from compost and the chitinolytic Clostridium paraputrificum from beach soil and Ruminococcus albus was isolated from cow rumen. After isolation, novel cellulase and xylanase genes from these anaerobes were cloned and expressed in Escherichia coli. The properties of the cloned enzymes showed that some of them were the components of the enzyme (cellulase) complex, i.e., cellulosome, which is known to form complexes by binding cohesin domains on the cellulase integrating protein (Cip: or core protein) and dockerin domains on the enzymes. Several dockerin and cohesin polypeptides were independently produced by E. coli and their binding properties were specified with BIAcore by measuring surface plasmon resonance. Three pairs of cohesin-dockerin with differing binding specificities were selected. Two of their genes encoding their respective cohesin polypeptides were combined to one gene and expressed in E. coli as a chimeric core protein, on which two dockerin-dehydrogenase chimeras, the dockerin-formaldehyde dehydrogenase and the dockerin-NADH dehydrogenase are planning to bind for catalyzing $CO_2$ reduction to formic acid by feeding NADH. This reaction may represent a novel strategy for the reduction of the green house gases. Enzymes from the anaerobes were also expressed in tobacco and rice plants. The activity of a xylanase from C. stercorarium was detected in leaves, stems, and rice grain under the control of CaMV35S promoter. The digestibility of transgenic rice leaves in goat rumen was slightly accelerated. C. paraputrificum was found to solubilize shrimp shells and chitin to generate hydrogen gas. Hydrogen productivity (1.7 mol $H_2/mol$ glucos) of the organism was improved up to 1.8 times by additional expression of the own hydrogenase gene in C. paraputrficum using a modified vector of Clostridiu, perfringens. The hydrygen producing microflora from soil, garbage and dried pelletted garbage, known as refuse derived fuel(RDF), were also found to be effective in converting biomass waste to hydrogen gas.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.326-336
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• 2016

Saccharomyces cerevisiae is one of the most employed cell factories for the production of bioproducts. Although monomeric hexose sugars constitute the preferential carbon source, this yeast can grow on a wide variety of nitrogen sources that are catabolized through central nitrogen metabolism (CNM). To evaluate the effects of internal perturbations on nitrogen utilization, we characterized strains deleted or overexpressed in GLT1, encoding for one of the key enzymes of the CNM node, the glutamate synthase. These strains, together with the parental strain as control, have been cultivated in minimal medium formulated with ammonium sulfate, glutamate, or glutamine as nitrogen source. Growth kinetics, together with the determination of protein content, viability, and reactive oxygen species (ROS) accumulation at the single cell level, revealed that GLT1 modulations do not significantly influence the cellular physiology, whereas the nitrogen source does. As important exceptions, GLT1 deletion negatively affected the scavenging activity of glutamate against ROS accumulation, when cells were treated with H₂O₂, whereas Glt1p overproduction led to lower viability in glutamine medium. Overall, this confirms the robustness of the CNM node against internal perturbations, but, at the same time, highlights its plasticity in respect to the environment. Considering that side-stream protein-rich waste materials are emerging as substrates to be used in an integrated biorefinery, these results underline the importance of preliminarily evaluating the best nitrogen source not only for media formulation, but also for the overall economics of the process.

• 한국도서관정보학회지
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• 제36권4호
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• pp.349-364
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• 2005

제7차 교육과정은 어린이의 개성과 창의성 개발을 중시하고 학생의 자발적인 활동과 디지털 자료를 활용하는 교수-학습 방법을 권장하고 있다. 학생 중심의 자기 주도적 학습이 강조되고 스스로 문제해결 능력을 배양하기 위해 컴퓨터와 정보통신기술의 패러다임을 기반으로 어린이도서관에서도 디지털 자료실의 이용현황과 이용자 만족도 및 요구사항을 분석함으로 향후 디지털 자료실 운영 시 고려할 사항을 제시할 필요가 있다. 이에 본 연구에서는 2003년 2월에 개관한 노원어린이도서관 디지털 자료실을 대상으로 운영 및 이용 현황을 분석함으로써 어린이도서관 디지털 자료실의 효과적인 이용을 통한 운영시 고려할 사항을 제시하고 보다 적극적인 정보서비스를 제공하는데 토대를 마련하고자 한다. 2장에서는 어린이도서관의 개념과 어린이도서관 내 디지털자료실의 개념적 모델을 정립하고3장에서는 노원어린이도서관 디지털 자료실의 운영현황을 분석하고 디지털자료실 이용자초등학생 100명을 대상으로 이용현황 및 요구사항을 분석한다. 4장에서는 분석을 통해 나타난 이용현황 및 요구사항을 기반으로 어린이도서관 디지털자료실 운영 시 고려해야 할 사항을 제시하고 결론을 맺고자 한다.

• 청정기술
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• 제22권4호
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• pp.250-257
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• 2016

As an attempt to replacing petroleum-based chemicals with bio-based ones, synthesis of furfural from biomass-derived xylose attracts much attention in recent days. Conventionally, furfural from xylose has been produced via the utilization of highly corrosive, toxic, and environmentally unfriendly mineral acids such as sulfuric acid or hydrochloric acid. In this study, microwave-assisted biphasic reaction process in the presence of novel bio-based heterogeneous acid catalysts was developed for the eco-benign and effective synthesis of furfural from xylose. The microwave was irradiated for reaction acceleration and a biphasic system consisting of $H_2O$ : MIBK (1 : 2) was designed for continuous extraction of furfural into the organic phase in order to reduce the undesired side products formed by decomposition/condensation/oligomerization in the acidic aqueous phase. Moreover, sulfonated amorphous carbonaceous materials were prepared from wood powder, the most abundant lignocellulosic biomass. The prepared catalysts were characterized by FT-IR, XPS, BET, elemental analysis and they were used as bio-based heterogeneous acid catalysts for the dehydration of xylose into furfural more effectively. For further optimization, the effect of temperature, reaction time, water/organic solvent ratio, and substrate/catalyst ratio on the xylose conversion and furfural yield were investigated and 100% conversion of xylose and 74% yield of furfural was achieved within 5 h at $180^{\circ}C$. The bio-based heterogeneous acid catalysts could be used three times without any significant loss of activity. This greener protocol provides highly selective conversion of xylose to furfural as well as facile isolation of product and bio-based heterogeneous acid catalysts can alternate the environmentally-burdened mineral acids.

• 한국학교ㆍ지역보건교육학회지
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• 제14권2호
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• pp.47-57
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• 2013

Backgrounds: According to a high interest in health these days, a bicycle is being highlighted as a means of leisure activity and auxiliary transportation means. Along with an increase in this interest, there is tendency that even the width of bicycle utilization gets diverse and that bicycle accident is also rapidly growing. Purpose: The purpose of this study was to suggest a prevention measure available for reducing occurrence of bicycle accidents by analyzing the Epidemiological characteristics according to age and injury severity in bicycle-injured patients. Methods: This study analyzed 115 materials suitable for this study by examining a medical record of patients, who received the hospitalized treatment owing to bicycle accident from January 1, 2011 to December 31 at one general hospital in S city. Results: The male-and-female ratio of bicycle-injured patients stood at about 7:3. The average age was 38.14(${\pm}21.59$) years old. The largest injury happened in 40-59 years old(31.3%). The averagely hospitalized period was 17.33(${\pm}18.12$) days. Injury severity was the ratio of 2:8 in a mild case versus a serious case. As for drinking and smoking characteristics according to age, the injury occurred the most in between 40 and 59 years old for people with drinking and smoking and in under 19 years for people without drinking and smoking. According to injury severity, the ratio of serious case got higher in the higher age. This was indicated(${\chi}^2$=15.675, p=0.001) to be statistically significant. Conclusion: In this study, a bicycle accident was indicated to have high injury risk in young children, adolescents, and old-age group. Prevention measure and publicity are demanded for reducing injury occurrence such as the prevention education for bicycle accident and wearing protective gear focusing on the ages of corresponding to groups with risk of injury occurrence. In addition, the security of safe movement route is necessarily demanded in order to increase application level as an auxiliary transportation means.

• 한국문헌정보학회지
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• 제54권1호
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• pp.175-195
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• 2020

본 연구의 목적은 대학도서관의 교수학습 활동 지원을 위한 데이터 큐레이션 서비스를 제안하는 연구이다. 이를 위해 데이터 큐레이션이라는 개념에 대한 정리와 대학도서관, 교수학습 정보 데이터 큐레이션을 어떻게 이해해야 하는지 정리하고 도서관 정보서비스의 전통적 연장선에서 대학도서관 데이터 큐레이션 서비스 활용을 제안하고자 했다. 연구결과로 데이터 큐레이션은 데이터의 활용에 더욱 방점을 찍어 이해하는 것이 타당하다고 제안하고 대학도서관은 모기관인 대학의 교육서비스에 기여하는 가시적인 효과를 위해서도 교수학습활동 자료의 기관 리포지터리 구축을 통한 데이터 큐레이션 서비스가 필요함을 역설했다. 이를 위해 다섯 단계의 교수학습 정보 데이터 큐레이션 프레임워크를 제시함으로써 연구를 마무리했다.

• 농촌지도와개발
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• 제7권1호
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• pp.193-208
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• 2000

이 연구는 우리 나라에 자생하고 있거나 도입재배되고 있는 식물 중 향료 및 약료적 이용성을 함께 지닌 자원식물종을 조사하여 이들 식물종에 대한 체계적 ${\cdot}$ 종합적인 연구를 위한 자료를 확보하기 위한 목적으로 수행되었다. 이 연구에서는 선행연구(변과 박$^{1)}$, 1999)에서 조사 ${\cdot}$ 분류하였던 우리 나라에 분포하고 있거나 또는 재배되고 있는 식물 중 향료자원화의 가능성이 있다고 조사된 방향성식물 초 69과(科), 1039종(種)을 중심으로 동의보감 등의 고문헌에 약재로 제시된 자원식물과 한방 상용약에 가용할 수 있는 약재로 이용되고 있는 방향성식물종을 비교 ${\cdot}$ 검토하는 한편 이들 방향성식물의 생활요품 및 식향, 조리용, 약료로서의 이용성도 함께 검토하였다. 연구결과 다음과 같은 사항을 확인할 수 있었다. 즉, 우리 나라에 서식 또는 재배되고 있는 향료식물 중 전통의약물의 원료로 이용되고 있는 식물이 매우 높을 뿐만 아니라, 주요한 한방 상용약의 재료로 대부분 가용할 수 있는 것으로 나타났다. 연구 결과 식물이 함유하고 있는 방향성물질과 인체에 미치는 약리적 효과는 상관관계가 매우 깊은 것으로 보여지며, 그와 관련된 보다 심도 있는 후속연구의 필요성이 제기되고 있다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 추계학술대회 초록집
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• pp.75.2-75.2
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• 2010

Polymer electrolyte fuel cells (PEFCs) have received a lot of attention as a power source for both stationary and mobile applications due to their attractive feature. In general, the performance of PEFCs is highly affected by the property of the electrodes. A PEFC electrode essentially consists of a gas diffusion layer and a catalyst layer. The gas difusion layer is highly porous and hydrophobicized with PTFE polymer. The catalyst layer usually contains electrocatalyst, proton conducting polymer, even PTFE as additive. Particularly, the proton conducting ionomer helps to increase the catalytic activity at three-phase boundary and catalyst utilization. Futhermore, it helps to retain moisture, resulting in preventing the electrodes from membrane dehydration. The most widely used proton conducting ionomer is perfluorinated sulfonic acid polymer, namely, Nafion from DuPont due to its high proton conductivity and good mechanical property. However, there are great demands for alternative ionomers based on non-fluorinated materials in terms of high temperature availability, environmental adaptability and production cost. In this study, the electrodes with the various content of the sulfonated poly(ether sulfone) ionomer in the catalyst layer were prepared. In addition, we evaluated electrochemical properties of the prepared electrodes containing the various amount of the ionomers by using the cyclic voltammetry and impedance spectroscopy to find an optimal ionomer composition in the catalyst layer.

• 펄프종이기술
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• 제39권1호
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• pp.38-47
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• 2007

This study was carried out to develop a new application field and obtain the basic data of citrus peel as waste in Jeju island and traditional Hanji for producing functional Hanji. The results measuring physical and optical properties, water vapor permeance and antibacterial activity are as follows. It was revealed that apparent density go as down but bulk raise up in the structural view of Hanji with increasing of the addition various Korean citrus peel (citrus unshiu, cheonggyun and hanrabong peel, and citrus unshiu peel powder) percentages, and that the density of Hanji added citrus unshiu peel was higher, but bulk was lower in compared with Hanji added other kinds of peel. Those Hanji added citrus unshiu peel, cheonggyun peel, hanrabong peel and citrus unshiu powder were very great not only in the strength (breaking length, burst index, tear index and folding endurance) but also in water vapor permeant rate in comparison with Hanji. The pHs of Hanji were neutrality (7 to 8). The brightness of the Hanji added various citrus peel percentages was low in compared to Hanji, and the 40% addition of hanrabong peel was the lowest. When 40% hanrabong peel was added to Hanji, it was very yellow in the color degree. When cheonggyun peel was added to Hanji manufacture, water vapor permeant rate was highly effective. It is known that vacant space of intrafiber was reduced by image analysis of Hanji and the additions of peel of citrus unshiu, cheonggyun and hanrabong were distributed equally in the interior of Hanji. The antibacterial activity of Hanji added citrus unshiu peel is more than 98%. After all, it would be able to increase utilization of Hanji, extensively. Namely, production of high quality Hanji added functional materials is expected for new valuable industry of citrus peel and Hanji.