• 대한화학회지
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• 제39권12호
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• pp.902-909
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• 1995

Ion trap 질량분석기와 Quadrupole 질량분석기를 이용한 밀가루 중 유기인제(dimethoate, diazi-non, parathion-methyl, fenithion, malathion) 및 인삼 중 BHC 이성질체들의 정성 정량 분석에 대한 유용성을 평가하기 위해 검출한계를 구하고, 검량선을 작성하였다. 이온화 방법으로 EI와 CI를 사용하여 mass fragmentation pattern을 비교하여 정성분석에 적용하였으며, scan과 selected ion monitoring(SIM) mode로 미량분석법으로서의 유용성을 평가하였다. 검출한계는 ion trap에서 scan시 0.008-0.225ng이었으며, quadru-pole에서는 scan시 0.23-3.1ng, SIM 측정시 0.032-0.68ng으로ion trap이 quadrupole의 scan시 보다 2-300배, SIM보다 4-30배까지 검출한계가 낮았다. 검량선의 직선성은 ion trap과 quadrupole모두 0.9이상이었다. 밀가루 추출물은 Bio-Bead S-X3를 사용하여 정제하였고, 인삼은 Florisil column으로 정제하였다. 5종의 유기인제의 회수율은 80% 이상이었으며, BHCs의 회수율은 60-90%이었다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3645-3651
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• 2012

Conventional pap smear (CPS) examination has been the mainstay for early detection of cervical cancer. However, its widespread use has not been possible due to the inherent limitations, like presence of obscuring blood and inflammation, reducing its sensitivity considerably. Automated methods in use in developed countries may not be affordable in the developing countries due to paucity of resources. On the other hand, manual liquid based cytology (MLBC) is a technique that is cost effective and improves detection of precursor lesions and specimen adequacy. Therefore the aim of the study was to compare the utility of MLBC with that of CPS in cervical cancer screening. A prospective study of 100 cases through MLBC and CPS was conducted from October 2009 to July 2010, in a Medical College in India, by two independent pathologists and correlated with histopathology (22 cases). Morphological features as seen through MLBC and CPS were compared. Subsequently, all the cases were grouped based on cytological diagnosis according to two methods into 10 groups and a subjective comparison was made. In order to compare the validity of MLBC with CPS in case of major diagnoses, sensitivity and specificity of the two methods were estimated considering histological examination as the gold standard. Increased detection rate with MLBC was 150%. The concordance rate by LBC/histopathology v/s CPS/histopathology was also improved (86% vs 77%) The percentage agreement by the two methods was 68%. MLBC was more sensitive in diagnosis of LSIL and more specific in the diagnosis of inflammation. Thus, MLBC was found to be better than CPS in diagnosis of precursor lesions. It provided better morphology with increased detection of abnormalities and preservation of specimen for cell block and ancillary studies like immunocytochemistry and HPV detection. Therefore, it can be used as alternative strategy for cervical cancer prevention in limited resource settings.

• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1563-1570
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• 2010

To construct a highly sensitive detection system for endocrine disruptors (EDs), we have compared the activity of promoters with the n-alkane-inducible cytochrome P450 gene (ALK1), isocitrate lyase gene (ICL1), ribosomal protein S7 gene (RPS7), and the translation elongation factor-1${\alpha}$ gene (TEF1) for the heterologous gene in Yarrowia lipolytica. The promoters were introduced into the upstream of the lacZ or hERa reporter genes, respectively, and the activity was evaluated by ${\beta}$-galactosidase assay for lacZ and Western blot analysis for hER${\alpha}$. The expression analysis revealed that the ALK1 and ICL1 promoters were induced by n-decane and by EtOH, respectively. The constitutive promoter of RPS7 and TEF1 showed mostly a high level of expression in the presence of glucose and glycerol, respectively. In particular, the TEF1 promoter showed the highest ${\beta}$-galactosidase activity and a significant signal by Western blotting with the anti-estrogen receptor, compared with the other promoters. Moreover, the detection system was constructed with promoters linked to the upstream of the expression vector for the hER${\alpha}$ gene transformed into the Y. lipolytica with a chromosome-integrated lacZ reporter gene under the control of estrogen response elements (EREs). It was indicated that a combination of pTEF1p-hER${\alpha}$ and CXAU1-2XERE was the most effective system for the $E_2$-dependent induction of the ${\beta}$-galactosidase activity. This system showed the highest ${\beta}$-galactosidase activity at $10^{-6}\;M\;E_2$, and the activity could be detected at even the concentration of $10^{-10}\;M\;E_2$. As a result, we have constructed a strongly sensitive detection system with Y. lipolitica to evaluate recognized/suspected ED chemicals, such as natural/synthetic hormones, pesticides, and commercial chemicals. The results demonstrate the utility, sensitivity, and reproducibility of the system for identifying and characterizing environmental estrogens.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.7123-7127
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• 2015

Purpose: To determine the utility of secondary circulating prostate cells for predicting early biochemical failure after radical prostatectomy for prostate cancer and compare the results with the Walz nomagram. Materials and Methods: A single centre, prospective study of men with prostate cancer treated with radical prostatectomy between 2004 and 2014 was conducted, with registration of clinical-pathological details, total serum PSA pre-surgery, Gleason score, extracapsular extension, positive surgical margins, infiltration of lymph nodes, seminal vesicles and pathological stage. Secondary circulating prostate cells were obtained using differential gel centrifugation and assessed using standard immunocytochemistry with anti-PSA. Biochemical failure was defined as a PSA >0.2ng/ml, predictive values werecalculated using the Walz nomagram and CPC detection. Results: A total of 326 men participated, with a median follow up of 5 years; 64 had biochemical failure within two years. Extracapsular extension, positive surgical margins, pathological stage, Gleason score ${\geq}8$, infiltration of seminal vesicles and lymph nodes were all associated with higher risk of biochemical failure. The discriminative value for the nomogram and circulating prostate cells was high (AUC >0.80), predictive values were higher for circulating prostate cell detection, with a negative predictive value of 99%, sensitivity of 96% and specificity of 75%. Conclusions: The nomagram had good predictive power to identify men with a high risk of biochemical failure within two years. The presence of circulating prostate cells had the same predictive power, with a higher sensitivity and negative predictive value. The presence of secondary circulating prostate cells identifies a group of men with a high risk of early biochemical failure. Those negative for secondary CPCs have a very low risk of early biochemical failure.

• 대한세포병리학회지
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• 제8권1호
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• pp.27-34
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• 1997

Pneumocystis carinli is an established cause of pulmonary infections in immuno-compromised hosts. Several cytoiogical stains, such as Papanicolaou, Gomori methenamine sliver(GMS) and Diff-Quik have been used for detection of the organism, but occasionally can be laborious and, due to a degree of nonspecificity, may be misleading. We evaluated the diagnostic utility of immunocytochenmical stains that recognize P. carinii in bornchoalveolar lavage from experimentally Induced P. carinii pneumonia rats(n=15). In audition to routine stains for diagnosis by morphologic recognition of P. carinii on Papanicolaou, GMS and Diff-Quik stains, bronchoalveolar lavage samples were reacted with immunocytochemical stains using monoclonal antibodies(MAB) 092 and 902. In bronchoalveolar lavage P. carinii organisms were detected In 9 of 10 cases(90%) using each MAB 092 and 902, whereas GMS and Diff-Quik stains demonstrated P. carinii in 13(86%) and 11(73%) of 15 cases respectively. In lung tissue specimens(n=15) P. carinii organisms were well identified on GMS stain and immunohistochemical stains using MAB 092 and 902 in ail cases. We believe that the immunocytochemical staining using MAB 092 and/or 902 is a very useful and diagnostic tool In addition to GMS and Diff-Qulk stain to detect P. carinii organisms in bronchoalveolar lavage.

• 비파괴검사학회지
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• 제21권6호
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• pp.607-611
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• 2001

초음파검사 시스템은 검사자, 검사장비, 검사절차서로 구성되어진다. 따라서 각각의 구성의 성능에 따라 초음파검사의 신뢰도가 좌우되며, 또한 검사환경이나 검사체의 재질, 결함의 유형에 따라서도 검사의 신뢰도는 영향을 받게된다. 이러한 다양한 인자들로 인해 비파괴검사의 신뢰도를 평가하는 것이 쉽지 않다. 본 연구에서는 로지스틱 모델과 몬테 카를로 시뮬레이션을 이용하여 초음파검사의 신뢰도를 척도할 수 있는 검출확률을 평가하고자 한다. 신뢰도 평가의 유용성을 검증하기 위해 원진배관에 대한 순회시험의 데이터에 신뢰도 평가 모델을 적용하였다.

• Parasites, Hosts and Diseases
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• 제55권6호
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• pp.607-611
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• 2017

Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.

• 대한원격탐사학회지
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• 제36권5_4호
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• pp.1231-1244
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• 2020

Landsat 위성은 지구표면을 장기간 관측한 대표적인 광학위성으로 재난 대비/복구 모니터링, 토지 이용 변화, 변화 탐지, 시계열 모니터링 등의 장기적인 변화에 활용하기 적합한 위성이다. 본 연구에서는 간단하고 효율적으로 구름을 탐지 및 제거하기 위해 QA밴드를 이용하여 구름 및 구름 그림자를 탐지하였다. 그 다음, 참조영상의 화소값을 직접 참조하는 것이 아닌 복원을 수행할 영상 내 화소값으로 복원을 수행하는 SSG 알고리즘을 통해 영상의 결측영역을 복원하였다. 본 연구를 통하여 지표를 관측하는 기존의 가시광선 영역뿐만 아니라 열 파장대역의 다양한 토지피복 상태의 정보를 복원하여 정량·정성적으로 평가함으로써 변형된 SSG 알고리즘의 활용 가능성을 제시하고자 하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제18권6호
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• pp.447-456
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• 2014

Until now, a disease-modifying therapy (DMT) that has an ability to slow or arrest Alzheimer's disease (AD) progression has not been developed, and all clinical trials involving AD patients enrolled by clinical assessment alone also have not been successful. Given the growing consensus that the DMT is likely to require treatment initiation well before full-blown dementia emerges, the early detection of AD will provide opportunities to successfully identify new drugs that slow the course of AD pathology. Recent advances in early detection of AD and prediction of progression of the disease using various biomarkers, including cerebrospinal fluid (CSF) $A{\beta}_{1-42}$, total tau and p-tau181 levels, and imagining biomarkers, are now being actively integrated into the designs of AD clinical trials. In terms of therapeutic mechanisms, monitoring these markers may be helpful for go/no-go decision making as well as surrogate markers for disease severity or progression. Furthermore, CSF biomarkers can be used as a tool to enrich patients for clinical trials with prospect of increasing statistical power and reducing costs in drug development. However, the standardization of technical aspects of analysis of these biomarkers is an essential prerequisite to the clinical uses. To accomplish this, global efforts are underway to standardize CSF biomarker measurements and a quality control program supported by the Alzheimer's Association. The current review summarizes therapeutic targets of developing drugs in AD pathophysiology, and provides the most recent advances in the clinical utility of CSF biomarkers and the integration of CSF biomarkers in current clinical trials.

• 한국위성정보통신학회논문지
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• 제10권1호
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• pp.49-55
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• 2015

인지무선 네트워크에서 스펙트럼 센싱은 우선사용자에게 간섭을 주지 않기 위해 기본적으로 수행해야 하는 단계이다. 스펙트럼 센싱에 요구되는 샘플 수는 2차 사용자의 성능에 직접적으로 영향을 주기 때문에, 2차 사용자의 성능과 우선사용자에 대한 간섭은 트레이드오프 관계에 있다. 스펙트럼 센싱에 필요한 샘플 수는 요구되는 오검출 확률, 검출확률 및 우선 사용자의 최소 요구 SNR로 부터 얻어진다. 우선 사용자 센싱에 요구되는 SNR은 2차 사용자의 전송반경과 관련 있기 때문에, 2차사용자들을 모아 센싱집합으로 구성하고 요구되는 전송영역을 최소화시킴으로써 스펙트럼 센싱에 요구되는 우선사용자의 SNR을 완화시킬 수 있다. 따라서 스펙트럼 센싱에 필요한 최소 샘플 수를 줄임으로써 인지무선 네트워크의 전송량을 향상시킬 수 있다. 본 논문에서는 이를 위해 센싱집합인 클러스터링을 통해 게임이론으로 클러스터의 크기에 따라 얻는 이득과 손실을 트레이드오프로 디자인하고, 시뮬레이션을 통해 제안된 방법의 성능을 확인한다.