• BMB Reports
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• 제36권3호
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• pp.299-304
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• 2003

For deciphering the cyclic guanosine monophosphate (cGMP) signaling pathway, we employed chemical proteomics to identify the novel target molecules of cGMP. We used cGMP that was immobilized onto agarose beads with linkers directed at three different positions of cGMP. We performed a pull-down assay using the beads as baits on tissue lysates and identified 9 proteins by MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) mass spectrometry. Some of the identified proteins were previously known cGMP targets, including cGMP-dependent protein kinase and cGMP-stimulated phosphodiesterase. Surprisingly, some of the co-precipitated proteins were never formerly reported to associate with the cGMP signaling pathway. The competition binding assays showed that the interactions are not by nonspecific binding to either the linker or bead itself, but by specific binding to cGMP. Furthermore, we observed that the interactions are highly specific to cGMP against other nucleotides, such as cyclic adenosine monophosphate (cAMP) and 5'-GMP, which are structurally similar to cGMP. As one of the identified targets, MAPK1 was confirmed by immunoblotting with an anti-MAPK1 antibody. For further proof, we observed that the membrane-permeable cGMP (8-bromo cyclic GMP) stimulated mitogen-activated protein kinase 1 signaling in the treated cells. Our present study suggests that chemical proteomics can be a very useful and powerful technique for identifying the target proteins of small bioactive molecules.

• 한국산학기술학회논문지
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• 제14권4호
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• pp.1810-1819
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• 2013

본 연구는 웃음요법 프로그램이 농촌 여성노인의 혈압, 우울 및 삶의 질에 미치는 효과를 규명하기 위한 비동등성 대조군 전후 유사 실험 연구이다. 연구대상자는 S면에 살고 있는 65세 여성노인으로 실험군 24명, 대조군 25명으로 총 49명 이었다. 실험처치는 2011년 1월 5일부터 4월 5일까지 13주간 1주일에 2회, 매회 60분간 실시하였으며, 중재효과를 비교분석하기 위해 t-검정으로 분석하였다. 연구결과 웃음요법은 농촌 여성노인의 수축기 혈압을 낮추고 우울을 감소시키며 삶의 질을 높이는 것으로 나타나 여성노인의 건강수준을 유지 증진시키는데 기여하는 유용한 프로그램으로 임상이나 지역사회에서 간호 중재방법으로 활용될 수 있을 것으로 기대된다.

• 한국수정란이식학회지
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• 제23권2호
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• pp.67-76
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• 2008

Since the birth of Dolly using fully differentiated somatic cells as a nuclear donor, viable clones were generated successfully in many mammalian species. These achievements in animal cloning demonstrate developmental potential of terminally differentiated somatic cells. At the same time, the somatic cell nuclear transfer (SCNT) technique provides the opportunities to study basic and applied biosciences. However, the efficiency generating viable offsprings by SCNT remains extremely low. There are several explanations why cloned embryos cannot fully develop into viable animals and what factors affect developmental potency of reconstructed embryos by the SCNT technique. The most critical and persuasive explanation for inefficiency in SCNT cloning is incomplete genomic reprogramming, such as DNA methylation and histone modification. Numerous studies on genomic reprogramming demonstrated that incorrect DNA methylation and aberrant epigenetic reprogramming are considerably correlated with abnormal development of SCNT cloned embryos even though its mechanism is not fully understood. The SCNT technique is useful in cloning farm animals because pluripotent stem cells are not established in farm animal species. Therapeutic cloning combined with genetic manipulation will help to control various human diseases. Also, the SCNT technique provides a chance to overcome excessive demand for the organs by production of transgenic animals as xenotransplantation resources. Here, we describe the factors affecting the efficiency of generating cloned farm animals by the SCNT technique and discuss future directions of animal cloning by SCNT to improve the cloning efficiency.

• 보건교육건강증진학회지
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• 제31권5호
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• pp.147-158
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• 2014

Objectives: This study aims to examine the association of depression, suicidal behaviors with smartphone use behaviors among Korean adolescents. Methods: We analyzed data from 'Youth Health and Life Skills Survey' in 2013. The survey was based on self-reported questionnaires distributed to 915 grade 7th and 8th students at four middle schools in Gangdong-gu, Seoul, Korea in 2013 to evaluate the effects of 'Youth Health and Life Skills' program. Depression and suicidal behaviors were measured in terms of the experience rate, and smartphone use behaviors were measured with average hours of smartphone use a day and overindulged behaviors in smartphone use by using 5-point Likert scales. We examined the association of depression and suicidal behaviors with smartphone use behaviors by using t-test and regression analyses. Results: 21.5% of respondents have ever felt depressed or hopeless to lose interest in school life almost everyday for 2 weeks or longer in the past year. The experience rate of suicidal ideation, suicide plan, and suicidal attempt were 21.1%, 6.1%, and 5.7%, respectively. The mean of average hours of smartphone use a day was 3.9 hours, and the mean score of overindulged behaviors in smartphone use was 2.99. The students who have experienced depression and suicidal behaviors were more likely to have more smartphone using time and higher scores of overindulged behaviors in smartphne use than those who have not. Conclusion: We concluded that for preventing addictive or problematic smartphone use behaviors among adolescents we should consider and develop more positive strategies for the moderate use of smartphone than regulatory measures, which include the implementation of skill-based health education program including life skills at schools and the development of smartphone applications useful for resolving mental stress and promoting mental health.

• 한국어병학회지
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• 제33권2호
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• pp.153-161
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• 2020

Cytochrome P450(CYP) gene is involved in the biotransformation of drugs and environmental pollutants. In this study, we analyzed the nucleotide sequence of the Anguilla japonica CYP1(AjCYP1) family gene and examined the relative expression of AjCYP1A, AjCYP1B and AjCYP1C1 in response to the exposure to environmental pollutants. After exposure to B[a]P 20mg/kg bw, the expression of AjCYP1 family gene increased over time. Among four tissues examined (liver, spleen, gill and kidney), AjCYP1 family gene was expressed significantly in the kidney. Compared with the control group, AjCYP1A was expressed about 5-fold at 48 hr, AjCYP1B about 6-fold at 24 hr, and AjCYP1C1 about 4-fold at 24 hr. However, after exposure to B[a]P 200mg/kg bw, AjCYP1A did not change in all tissues. On the other hand, AjCYP1B was expressed at about 4-fold at 24 hr in the spleen and 4-fold at 48 hr in the gill. Finally AjCYP1C1 was expressed 3.7-fold and 4.3-fold in the spleen and kidneys at 48 hr, respectively. Taken together, our results suggest that the expression of AjCYP1 gene in eel tissues might be used as a useful tool to assess the exposure to environmental pollutants in aquaculture system.

• 한국산업정보학회논문지
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• 제16권5호
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• pp.107-114
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• 2011

신제품 개발 또는 개선 과정에 있어서 내구 수명의 평가는 장기간이 소요되는 작업으로서, 제품의 개발 또는 개선 기간에 큰 영향을 미치게 된다. 따라서 고장 메커니즘을 분석하여 제품의 신뢰성을 정확히 평가하는 것은 제품의 초기 시장 진입 기간을 단축하여 시장 경쟁력을 강화하는데 중요한 요소로 작용하게 된다. 본 논문에서는 트랙터의 무상보증기간에 이루어진 고장 데이터에 근거하여 주요 고장 부품에 대한 고장 메커니즘 분석을 통하여 고장형태를 규명하고, 신뢰성 분석을 통하여 파워트레인에 대한 B10 수명을 향상시키기 위한 대상 부품을 규명하였다.

• 한국정밀공학회지
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• 제17권12호
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• pp.94-100
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• 2000

This paper presents a methodology for In-Process monitoring of tool wear by using ultrasonic sensor in turning operation. An integrated single ultrasonic transducer operation at a frequency of 10MHz is placed in contact with the insert tip. The change in amount of the reflected energy from the nose and flank of the tool can be related to the level of tool wear and the mechanical integrity of the tool. As the results, the tool wear monitoring system based on the ultrasonic pulse-echo method was proposed, it is useful to determine a tool life and tool change time.

• 미생물학회지
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• 제12권1호
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• pp.37-51
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• 1974

Three strains which accumulated large quantities of L-hlutamic acid as the chief product of metabolism in the presence of glucose and inorhanic ammonium salt under the aerobic condition sere newly isolated. These strains have the general characters as follows: Gram-positive, short fod-shaped, non-sporulating, non-motile, and facultative anaerobe. A change of cell shates was not almost observed in their life cycles and neither a phenomenon of cell-elongation nor a pleomorphism was recognized in any cases. Together with rigid stability in Gram-stain, these characters as above indicated evidences that newly isolates would belong to genus Brevibacterium clearly differentiated from genus Corynebacterium. At the same time the newly isolates, in addition to nutritional requirement of biotin and thiamine, showed the distinctive character of requirement of special amino acid such as histidine or cysteine for their considerable growth. These physiological characters including nitrates reduction, reaction on milk, and slow formation of acid from sugar also were useful in confirming that these bacteria would be Brevibacterium ammoniagenes.

• 한국실내디자인학회논문집
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• 제15권5호
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• pp.202-208
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• 2006

The purpose of this study is to suggest architectural planning guideline for the improvement of the evacuation environment in a ward. To suggest of guideline, the problem of irrational domestic criteria is searched according to compare National fire code (NFPA101, Life safety code) with Korea building law. This study includes stairs, corridors and exit door etc. but excludes fire facilities. To verify effectiveness of suggested guideline, egress simulation is run that based on databases according to literature survey. The results of this study could be summarized as follow: Suggested guidelines are subdivision(fire partition), evacuation by elevator, secure useful waiting space of elevator hall and smokeproof enclosures, the corridor is divided by fire or smoke barriers corridor from hazardous room, only wheeled items are arranged in corridors of a ward story. The result of computer simulation for suggested guidelines verify that egress time can be decreased 20% or above.

• Journal of the Korean Statistical Society
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• 제30권1호
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• pp.89-98
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• 2001

Brown and Proschan(1983) introduced a model for imperfect repair. At each failure of a device, with probability p, it is repaired completely or replaced with a new device(perfect repair), and with probability 1-p, it is returned to the functioning state, but it is only recovered to its condition just prior to failure(imperfect repair or minimal repair). In this paper, we limit the number of consecutive minimal repairs by n. We find some useful properties about $\mu$$_{k}$, the expected time between the k-th and the (k+1)-st repair under he assumption that only minimal repairs are performed. Then, we assign cost to each repair and find the value of n which minimized the long-run average cost for a fixed p under the condition that the life distribution F os the device is DMRL.L.