• Fashion & Textile Research Journal
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• v.7 no.3
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• pp.321-326
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• 2005

The purpose of this study was to investigate the effect of body fat on energy metabolic response and subjective sensations under the hot environment. Fifteen female university students volunteered as subjects. We organized subjects into three groups: low body fat group(group L : less than 20% of body fat), medium body fat group(group M : 20%~30% of body fat) and high body fat group(group H: more than 30% of body fat). The experiment was conducted with $32^{\circ}C$, 60%RH. The subjects repeated 'Exercise' and 'Rest' period. The results of this study are as follows ; The oxygen uptake value of AM is higher than PM. The value of group H is the highest in three fat groups. But it showed group L is the highest in oxygen uptake per weight. %body fat is the lower, oxygen uptake is the higher. In Calorie, group L has higher value in AM in than in PM. In M group and group H, a value of PM is higher than AM. In group H, difference of AM and PM is the highest. From a view point of three groups, a value of group H is the highest. This support that calorie increases as oxygen uptake increase. The heart rate values of group L and group H are the higher in AM than in PM. This support that heart rate was relation to oxygen uptake. In all three groups, the value of blood pressure is higher in AM than in PM. Subjective sensations of temperature sensation, thermal comfort, and wetness sensation are higher in Am than in Pm. This explains that subject sensations are similar to experimental data, such as oxygen uptake, heart rate, blood pressure. In oxygen uptake, heart rate and blood pressure, general tend to showed higher AM than PM. This showed that heart rate, oxygen uptake increase in AM, as blood pressure increase, too. From a view point of %body fat, group H is higher than the others in oxygen uptake, heart rate and blood pressure.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.6
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• pp.679-686
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• 2010

To investigate the contribution of macroalgae to biogeochemical nutrients and carbon cycles, we measured the uptake rates of nutrients and $CO_2$ by Undaria pinnatifida using an incubation method in an acrylic chamber. From January to March 2010, U. pinnatifida was sampled at Ilkwang, a well-known area of macroalgae culture in Korea. The initial and final concentrations of nutrients, dissolved oxygen, total alkalinity, and pH of the chamber water were measured, and production/uptake rates were calculated using concentration changes, chamber volume, and incubation time. The production rate of dissolved oxygen by U. pinnatifida (n = 32) was about $5.4{\pm}4.0\;{\mu}mol\;g_{fw}^{-1\;}h^{-1}$. The uptake rate of total dissolved inorganic carbon (TDIC), calculated by total alkalinity and pH, was $7.9{\pm}6.5\;{\mu}mol\;g_{fw}^{-1}\;h^{-1}$. Nutrients uptake averaged $141.7{\pm}119.2$ nmol N $g_{fw}^{-1}\;h^{-1}$ and $15.0{\pm}9.1$ nmol P $g_{fw}^{-1}\;h^{-1}$. A positive linear correlation ($r^2$ = 9.6) existed between the production rate of dissolved oxygen and the uptake rate of total dissolved inorganic carbon, suggesting that these two factors serve as good indicators of U. pinnatifida photosynthesis. The relationships between fresh weight and uptake rates of nutrients and $CO_2$ suggested that younger specimens (<~50 g fresh weight) are much more efficient at nutrients and $CO_2$ uptake than are specimens >50 g. The amount of carbon uptake by the total biomass of U. pinnatifida in Korea during the year of 2008 was about 0.001-0.002% of global ocean carbon uptake. Thus, more research should be focused on macroalgae-based biogeochemical cycles to evaluate the roles and contributions of macroalgae to the global carbon cycle.

• The Korean Journal of Physiology and Pharmacology
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• v.1 no.4
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• pp.425-434
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• 1997

Many reports represent that angiotensin II (ANG II) caused a dose dependent biphasic effects on fluid transport in the proximal tubule. However, respective roles of different signaling pathways in mediating these effects remain unsettled. The aim of the present study was to examine signaling pathways at high doses of ANG II on the $Na^+$ uptake of primary cultured rabbit renal proximal tubule cells(PTCs) in hormonally defined serum-free medium. High concentrations of ANG II $(>10^{-9}\;M)$ inhibited $Na^+$ uptake and increased $[Ca^{2+}]_i\;level$ in the PTCs. However, low concentrations of $(<10^{-11}\;ANG\;II)$ stimulated $Na^+$ uptake and did not affect $[Ca^{2+}]_i\;level$. 8-(N, N-diethylamino)-octyl-3,3,5- trimethoxybenzoate (TMB-8), ethylene glycol-bis$({/beta}-amino\;ethyl ether)-N,N,N'$, N'-tetra acetic acid (EGTA), and nifedifine partially blocked the inhibitory effects of ANG II on $Na^+$ uptake. When ANG II and bradykinin (BK) were treated together, $Na^+$ uptake was further reduced $(88.47{\pm}1.98%\;of\;that\;of\;ANG\;II,\;81.85{\pm}1.84%\;of\;that\;of\;BK)$. In addition, W-7 and KN-62 blocked the ANG II-induced inhibition of $Na^+$ uptake. Arachidonic acid reduced $Na^+$ uptake in a dose-dependent manner. When ANG II and arachidonic acid were treated together, inhibitory effects on $Na^+$ uptake significantly exhibited greater reduction than that of each group, respectively. When PTCs were treated by mepacrine $(10^{-6}\;M)$ and AACOCF3 $(10^{-5}\;M)$ for 1 hr before the addition of $(<10^{-9}\;ANG\;II)$, the inhibitory effect of ANG II was reversed. In addition, econazole $(>10^{-6}\;M)$ blocked ANG II-induced inhibition of $Na^+$ uptake. In conclusion, the $[Ca^{2+}]_i$ (calcium-calmodulin-dependent kinase) and phospholipase $A_2\;(PLA_2)$ metabolites are involved in the inhibitory effects of ANG II on $Na^+$ uptake in the PTCs.

• Journal of Yeungnam Medical Science
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• v.7 no.1
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• pp.29-37
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• 1990

The effects of insulin and exercise on glucose uptake of skeletal muscle were investigated in soleus muscle isolated from low dose streptozotocin induced diabetic rats in vitro. Glucose uptake was assessed by measuring $^3H$-methylglucose uptake in vitro. Basal glucose uptake in diabetes was reduced by approximately one-third of the control value($5.6{\pm}0.73{\mu}Mol$/g/20min. in diabetes versus $8.4{\pm}0.77$ in control, P<0.01). There was also a significant decrease(P<0.01) in glucose uptake of diabetes at physiologic insulin concentration ($200{\mu}IU$/ml) by 40% ($6.1{\pm}1.20$ versus $10.0{\pm}0.81$). Furthermore, maximal insulin($20000{\mu}IU$/ml)-stimulated glucose uptake was 36% lower in diabetes as compared with control($7.3{\pm}1.29$ versus $11.4{\pm}1.29$, P<0.01). In contrast, exercise(1.0km/hr, treadmill running for 45min.) effect on glucose uptake was so dramatic in diabetes that glucose uptake at basal state was 8.4+1.09 and insulin stimulated-glucose uptake were $10.2{\pm}1.47$ and $11.9{\pm}1.64$, in 200 and $20000{\mu}IU$/ml added insulin, respectively. These results suggest that insulin insensitivity develops in skeletal muscle after 2 weeks of streptozotocin-induced diabetes, but these insensitivity was recovered significantly by single session of running exercise.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.3
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• pp.420-427
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• 2015

It is necessary to understand the cellular uptake and cytotoxicity of food-grade delivery systems, such as ${\beta}$-lactoglobulin (${\beta}$-lg) nanoparticles, for the application of bioactive compounds to functional foods. The objectives of this study were to investigate the relationships between the physicochemical properties of ${\beta}$-lg nanoparticles, such as particle size and zeta-potential value, and their cellular uptakes and cytotoxicity in Caco-2 cells. Physicochemical properties of ${\beta}$-lg nanoparticles were evaluated using particle size analyzer. Flow cytometry and confocal laser scanning microscopy were used to investigate cellular uptake and cytotoxicity of ${\beta}$-lg nanoparticles. The ${\beta}$-lg nanoparticles with various particle sizes (98 to 192 nm) and zeta-potential values (-14.8 to -17.6 mV) were successfully formed. A decrease in heating temperature from $70^{\circ}C$ to $60^{\circ}C$ resulted in a decrease in the particle size and an increase in the zeta-potential value of ${\beta}$-lg nanoparticles. Non-cytotoxicity was observed in Caco-2 cells treated with ${\beta}$-lg nanoparticles. There was an increase in cellular uptake of ${\beta}$-lg nanoparticles with a decrease in particle size and an increase in zeta-potential value. Cellular uptake ${\beta}$-lg nanoparticles was negatively correlated with particle size and positively correlated with zeta-potential value. Therefore, these results suggest that the particle size and zeta-potential value of ${\beta}$-lg nanoparticles play an important role in the cellular uptake. The ${\beta}$-lg nanoparticles can be used as a delivery system in foods due to its high cellular uptake and non-cytotoxicity.

• Korean Journal of Organic Agriculture
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• v.13 no.2
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• pp.211-221
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• 2005

This experiment was conducted to evaluate the effect of animal slurry on nitrogen uptake of hairy vetch and growth of chinese cabbage in cropping system. Hairy vetch was seeded on September 20 in 2003 . We examined the effect of cover crop(hairy vetch (Vicia villosa Roth) and slurry application(0, l00, 200kg N/ha) on yield and N uptake. Dry matter yield and nitrogen uptake of hairy vetch were measured. The chinese cabbage succeeding cover crop was harvested in 2004. The nitrogen uptake of hairy vetch was 84, 121, 148kg N/ha respectively, when the slurry application was 0, 100 and 200kg N/ha. In addition. N uptake of hairy vetch at the plot of 100kg and 200 kg N/ha slurry were 44% and 76% higher than that of the plot without slurry application respectively. Slurry application of hairy vetch could increase nitrogen uptake by application of green manure at the harvesting time. the content of organic matter and P-content of soil with hairy vetch plot was higher than that of fallow plot due to inhibition of soil erosion. The organic matter levels tend to improve with the addition of hairy vetch. Hairy vetch could improve soil quality by reducing erosion compared with bare fallow. The green manure of hairy vetch with animal slurry maintained soil nutrient and reduced nitrogen fertilizer of chinese cabbage. The hairy vetch residues decomposed rapidly releasing half of their residues within 40 days after burial. The yield of chinese cabbage was 90% in the plot of manure of hairy vetch compared with chemical fertilizer. The yield in the plot treated with green manure of hairy vetch and 50% of chemical fertilizer was reduced 5% less than that of chemical fertilizer. Therefore, it was estimated that the green manure of hairy vetch-chinese cabbage cropping system could reduce nitrogen chemical fertilizer as much as 84~148kgN/ha. the green manure of hairy vetch with animal slurry maintained soil nutrient and reduced nitrogen fertilizer of chinese cabbage.

• BMB Reports
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• v.39 no.4
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• pp.383-393
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• 2006

Little is known concerning the mechanisms responsible for the transplacental transfer of thiamine. So, the aim of this work was to characterize the placental uptake of thiamine from the maternal circulation, by determining the characteristics of $^3H$-thiamine uptake by a human trophoblast cell line (BeWo). Uptake of $^3H$-thiamine (50-100 nM) by BeWo cells was: 1) temperature-dependent and energy-independent; 2) pH-dependent (uptake increased as the extracellular medium pH decreased); 3) $Na^+$-dependent and $Cl^-$-independent; 4) not inhibited by the thiamine structural analogs amprolium, oxythiamine and thiamine pyrophosphate; 5) inhibited by the unrelated organic cations guanidine, N-methylnicotinamide, tetraethylammonium, clonidine and cimetidine; 6) inhibited by the organic cation serotonin, and by two selective inhibitors of the serotonin plasmalemmal transporter (hSERT), fluoxetine and desipramine. We conclude that $^3H$-thiamine uptake by BeWo cells seems to occur through a process distinct from thiamine transporter-1 (hThTr-1) and thiamine transporter-2 (hThTr-2). Rather, it seems to involve hSERT. Moreover, chronic (48 h) exposure of cells to caffeine ($1\;{\mu}M$) stimulated and chronic exposure to xanthohumol and iso-xanthohumol (1 and $0.1\;{\mu}M$, respectively) inhibited $^3H$-thiamine uptake, these effects being not mediated through modulation of the expression levels of either hThTr-1 or hSERT mRNA.

• Korean Journal of Clinical Laboratory Science
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• v.39 no.3
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• pp.249-255
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• 2007

While respiratory burst enhances neutrophil glucose utilization, many neutrophil functions are critically influenced by extracellular matrix interaction and phosphoinositide-3-OH kinase (PI3K) signaling. We thus evaluated the role of RGD integrin occupancy and PI3K inhibition on respiratory burst and [18F]FDG uptake of stimulated neutrophils. Human neutrophils were stimulated by 100 ng/mL phorbol-myristate-acetate (PMA), and respiratory burst was measured by cumulative luminescence with lucigenin. [18F]FDG uptake and total hexokinase activity was measured 20 min after PMA stimulation in the presence or absence of soluble RGD peptides (200 g/mL) and/or the PI3K inhibitor wortmannin (200 nM). PMA induced a 71.70.9 fold increase in neutrophil oxygen intermediate generation. [18F]FDG uptake was increased to $194.6{\pm} 3.7%$ and hexokinase activity to $145.0{\pm}2.0%$ of basal levels (both p<0.0005). RGD peptides attenuated respiratory burst activation to $35.6{\pm}0.2%$ (p<0.005), but did not inhibit stimulated [18F]FDG uptake or hexokinase activity. In contrast, without affecting respiratory burst activation, wortmannin inhibited PMA stimulated [18F]FDG uptake to $66.9{\pm}1.6%$ and hexokinase activity to $81.0{\pm}4.2%$ (both P<0.0005), demonstrating its dependence on PI3K activity. Neither RGD nor wortmannin reversed the other's inhibitory effect on stimulated [18F]FDG uptake and hexokinase activity or respiratory burst, which suggests the involvement of distinct signaling pathways. Neutrophil [18F]FDG uptake is enhanced by PMA through a mechanism that requires PI3K activity but is independent of integrin receptor occupancy or respiratory burst activation.

• Korean Journal of Veterinary Research
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• v.6 no.1
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• pp.24-30
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• 1966

In order to observe the effect of the injected radioactive iodine-$I^{131}$ on the uptake in thyroid of normal male guinea pigs and P.B.$I^{131}$ conversion ratio of $I^{131}$ in serum, 24 matured male guinea pigs were divided in 4 groups and $35{\mu}c$, $70{\mu}c$, $140{\mu}c$ and $280{\mu}c$ per kg of body weight respectively were injected subcutaneously. 1. The uptake rates of radioactveiodine-$I^{131}$ by external counts of thyroidal uptake reached the maximum level of uptake in 24 hours after injection. 2. As the injected amount increases, the uptake rates of maximum levels and release rate were increased. 3. Uptake rate in the removed thyroid have shown no statistical in the $35{\mu}c$ and $70{\mu}c$ groups of injected guinea pigs. 4. There was no statistical significance in $140{\mu}c$ and $280{\mu}c$ groups of injected guinea pigs. 5. P.B.$I^{131}$ conversion ratio of $I^{131}$ in serum was not in proportion to injected amounts: 61.0%(35), 70.2%(70), 75.3%(140) and 64.8%(280).

• Journal of Korean Foot and Ankle Society
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• v.15 no.4
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• pp.212-216
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• 2011

Purpose: This study was designed to analyze the usefulness of Single Photon Emission Computed Tomography/Computed Tomography (SPECT/CT) in diagnosing symptomatic accessory tarsal bones. Materials and Methods: Twenty four feet (16 patients) with symptomatic accessory navicular and/or os trigonum, who agreed to take SPECT/CT, were included in this study. Fifteen feet had accessory navicular, five had os trigonum, and four had both. According to the uptake in the SPECT/CT, 11 feet were classified into high and 13 into low uptake groups. The low uptake group was treated non-operatively, while the high uptake group received operations when initial conservative management failed. A modified Kidner procedure was performed for accessory navicular and arthroscopic excision was done for os trigonum. After a mean follow-up of 6.8 (range, 3~13) months, the American Orthopaedic Foot and Ankle Society (AOFAS) score and the Visual Analogue Scale (VAS) for pain were compared. Results: Patients in the high uptake group had a higher initial mean VAS score ($7.0{\pm}0.8$ vs $2.2{\pm}0.9$, p<0.05) and a lower initial mean AOFAS score ($45.9{\pm}9.2$ vs $83.9{\pm}4.2$, p<0.05) compared to the low uptake group. All patients in the low uptake group improved after non-operative treatment. Seven patients underwent operations and had a decreased VAS ($1.6{\pm}0.5$) and an increased AOFAS score ($88.3{\pm}1.8$) at the last follow-up. Four patients in the high uptake group demonstrated erratic symptoms. Conclusion: SPECT/CT can be a useful diagnostic tool and helpful in designing treatment plans for symptomatic accessory navicular and os trigonum.