• Title/Summary/Keyword: uptake$

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Analysis of Polyamine Transport of Young Spring Radish Cotyledons (무우의 유자엽에서 Polyamine의 수송 분석)

  • Cho, Bong-Heuy
    • Analytical Science and Technology
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    • v.11 no.2
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    • pp.130-134
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    • 1998
  • Polyamines were analyzed in young cotyledons of spring radish. The uptake rate of SPD were transported within 20 min lineary and reached the saturation phase after 1hr. The uptake rate of SPD decreased gradually with the time by the increasing amount of SPD inside the cells. The uptake of PA depends on the external pH. The optimal pH of PA uptake are pH 7.5. $K_m-$ and $V_{max}-$values depend on external pH also. The uptake rate of PA was inhibited by external KCI, which depolarized membrane potential in the cells.

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Characterization of Taurine Transport in Conditionally Immortalized Rat Brain Capillary Endothelial Cell Lines (조건적 불사화 흰쥐 뇌 모세혈관 내피세포주에서 Taurine 수송 특성)

  • 강영숙;이나영
    • Biomolecules & Therapeutics
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    • v.10 no.2
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    • pp.99-103
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    • 2002
  • Taurine has a neuroprotective action from oxidative stress in neural cell. In the present study, we studied taurine transport under basal and stressed conditions in conditionally immortalized rat brain capillary endothelial cell line (TR-BBB13) in vitro. The uptake of[$^3{H}$]taurine in the TR-BBB13 was increased by time-dependently and dependent on both Na$^{+}$ and Cl/ sup -/. Furthermore, $\beta$-alanine strongly inhibited the uptake of [TEX>$^3{H}$]taurine in the TR-BBB13. To study the effcts of oxidative stress on taurine transport, we used diethyl maleate (DEM) and lipopolysccharide (LPS). Diethyl maleate (DEM, $300\Mu\textrm{M}$) significantly reduced uptake of [TEX>$^3{H}$]taurine by time-dependently until 8 hr exposure in TR-BBB 13. But, the [TEX>$^3{H}$]taurine uptake was not changed by lipopolysccharide (LPS, 10 ng/ml) in TR-BBB13.3.

The Effect of Organic Solvent in the Dyeing of Silk Fiber (견섬유의 염색에 있어서 첨가용제의 영향( I ))

  • 황성민;윤남식;임용진;이동수;이인전
    • Textile Coloration and Finishing
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    • v.1 no.1
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    • pp.47-53
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    • 1989
  • The effect of organic solvent in the dyeing of silk fiber by acid dye was investigated. Acetophenone and benzyl alcohol were shown to be the most effective for the rate of dyeing of silk fiber by Milling Cyanine 5R (C.I. Acid Blue 113), a milling type acid dye, but, with benzyl alcohol, the equilibrium dye uptake was much lower than that in the absence of it. In the presence of solvent, maximum dye uptake shifted to lower temperature than 6$0^{\circ}C$, while without solvent, it was shown at about $60^\circ{C}$. When dyed by Orange II (C.I. acid Orange 7) under same condition equilibrium dye uptake of silk fiber was lower than that for milling type acid dye, and in the presence of benzyl alcohol, still much lower uptake resulted. All these fact reveals that organic solvents in the solvent-assisted dyeing of silk fiber broaden micelle spacings too much, resulting in increased rate of dyeing, and decreased equilibrium dye uptake, contrary to wool.

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Growth and Phosphate Uptake of the Toxic Dinoflagellate Gymnodinium catenatum Isolated from Yeosuhae Bay, South Korea (여수해만산 유독 와편모조류 Gymnodinium catenotum (Graham)의 용존태 무기인에 대한 성장 및 흡수)

  • Oh, Seok-Jin;Yoon, Yang-Ho;Yang, Han-Soeb
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.40 no.2
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    • pp.95-101
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    • 2007
  • We investigated the growth and phosphate uptake of a toxic dinoflagellate, Gymnodinium catenatum, isolated from Yeosuhae Bay, South Korea. A short-term phosphate uptake experiment revealed that its maximum uptake and the half-saturation constant were 1.39 pmol/cell/hr and $2.65{\mu}M$, respectively. In a semicontinuous culture, the maximum specific growth rate and minimum phosphorus cell quota of G. catenatum were 0.39/day and 1.27 pmol/cell, respectively. Thus, G. catenatum is a poor competitor in terms of inorganic nutrient use and is unlikely to form blooms in Yeosuhae Bay.

Carbon Storage and Uptake by Street Trees in Seoul

  • Jo, Hyun-Kil;Kim, Jin-Young;Park, Hye-Mi
    • Journal of Forest and Environmental Science
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    • v.34 no.2
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    • pp.162-164
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    • 2018
  • This study quantified the carbon storage and uptake by street trees in Seoul and explored suitable planting and management strategies. A systematic sampling model was used to select 50 plots to survey the structures of street trees. The average density and cover of street trees were approximately 5.8 trees/$100m^2$ and 12.1%, respectively. Trees with a dbh of less than 30 cm accounted for about 66.3% of the total number of trees surveyed. The total carbon storage and uptake by the street trees were approximately 103,641 t and 10,992 t/yr, respectively. The total carbon uptake equaled the amount of annual carbon emissions from driving of about 11,000 cars. Street tree planting and management strategies were proposed to enhance carbon uptake. They included multi-layered and multi-aged planting, securing ground and space for plant growth, and avoiding excessive tree pruning.

Effects of Herba Cirsii Extracts on Glucose Uptake in OP9 Cells (OP9 세포에서 포도당 흡수능에 대한 대계 추출물의 효과)

  • Kim, Mi Seong;Song, Je Ho
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.28 no.2
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    • pp.195-199
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    • 2014
  • Although the Herba Cirsii is known to posses beneficial health effects, the anti-diabetic effects and the mechanism of action have not been elucidated. In the present study we have shown that Herba Cirsii Extract (HCE) can stimulate glucose uptake in OP9 adipocytes. Unlike insulin, HCE did not stimulate the Ser473 phosphorylation and activation of Akt. The increasing effects of HCE on glucose uptake were inhibited by PD680509 and compound C pretreatment, which means that the glucose uptake effects by HCE were carried out by extracelluar signal-regulated kinase1/2(ERK1/2) and AMP-activated protein kinase (AMPK) activation. Further studies revealed that HCE stimulated glucose transport occurs through a mechanism involving ERK1/2 activation and AMPK activation.

Sugar and Amino Acid Transport in Yeast (효모세포의 당과 아미노산의 운반에 관한 연구 II)

  • 민경희;권영명
    • Korean Journal of Microbiology
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    • v.16 no.4
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    • pp.148-154
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    • 1978
  • Saccharomyces cerevisiae J170, a mutant, was used for $DL-^{14}C-leucine$ uptake during the sporulation and vegetative stage. $^{14}C-Leucine$ uptake into yeast cells appeared the highest at pH 6.0, indicating the same result of glucose transport, $^{14}C-Leucine$ uptake in sporulation period was higher than in growth phase, showing the evidence that leucine is more required for protein synthesis. This tendency has the evidence tht leucine is more required for protein synthesis. This tendency has the evidence that leucine is more required for protein synthesis. This tendency has been also supported from the result of Km values of leucine uptake in two stages of yeast. Leucine uptake was inhibited by 2,4-dinitrophenol in two stages of yeast. This means that leucine transport system is associated with energy dependent in both stages. The contents of all amino acid in growth phase cells were higher than those of sporulation stge cells, and those of methionine and tyrosine were showed in trace during the sporulation stage. In contrast, the content of glutamic acid in sporulation stage was compared with those of other amino acids.

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Effects of Gut Extract Protein and Insulin on Glucose Uptake and GLUT 1 Expression in HC 11 Mouse Mammary Epithelial Cells

  • Myung, K.H.;Ahn, B.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.8
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    • pp.1210-1214
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    • 2002
  • The large and rapid changes of glucose utilization in lactating mammary tissue in response to changes in nutritional state must be largely related by external signal of insulin. This also must be related with the quantity and composition of the diet in vivo. To characterize the mode of gut extract protein with insulin, in vitro experiment was conducted with HC11 cells. The gut extract protein has not only the same effect as insulin alone but also the synergistic effect with insulin in 2-Deoxy[3H] glucose uptake. Although the gut extract did not modulates glucose uptake via increasing the rate of translation of the GLUT1 protein, northern blot analysis indicated that the gut extract protein increased the expression of GLUT1 mRNA by a threefold and also there was a dose-dependent increase in the expression of GLUT1 mRNA. The gut extract protein is therefore shown to be capable of modulating glucose uptake by transcription level with insulin in HC 11 cells.

Water Uptake and Tensile Properties of Plasma Treated Abaca Fiber Reinforced Epoxy Composite

  • Paglicawan, Marissa A.;Basilia, Blessie A.;Kim, Byung Sun
    • Composites Research
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    • v.26 no.3
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    • pp.165-169
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    • 2013
  • This work presents the tensile properties and water uptake behavior of plasma treated abaca fibers reinforced epoxy composites. The composites were prepared by vacuum assisted resin transfer molding. The effects of treatment on tensile properties and sorption characteristics of abaca fiber composites in distilled water and salt solution at room temperature were investigated. The tensile strength of the composites increased with plasma treatment. With plasma treatment, an improvement of 92.9% was obtained in 2.5 min exposure time in plasma. This is attributed to high fiber-matrix compatibility. Less improvement on tensile properties of hybrid treatment of sodium hydroxide and plasma was obtained. However, both treatments reduced overall water uptake in distilled water and salt solution. Hydrophilicity of the fibers decreased upon plasma and sodium hydroxide treatment, which decreases water uptake.

P RELEASE AND UPTAKE ACCORDING TO INFLUENT ORGANIC LOADING IN BNR PROCESS

  • Kim, Kwang-Soo;Ahn, Chang-Hoon;Park, Jae-Kwang
    • Environmental Engineering Research
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    • v.10 no.6
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    • pp.265-268
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    • 2005
  • A batch-type study was conducted to investigate the phosphorus release and uptake under anaerobic and aerobic conditions according to organic loading changes. As organic loading increased, anaerobic P release increased but aerobic P uptake decreased. Where organic carbon contents remain high in aerobic conditions, PHB consumption within the microbial cells diminished, therefore it was found that in order to enhance P uptake rate, it should reach the endogenous growth stage where the entire organic loading was consumed.