Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Characterization of Taurine Transport in Conditionally Immortalized Rat Brain Capillary Endothelial Cell Lines

조건적 불사화 흰쥐 뇌 모세혈관 내피세포주에서 Taurine 수송 특성

  • 강영숙 (숙명여자대학교 약학대학) ;
  • 이나영 (숙명여자대학교 약학대학)
  • Published : 2002.06.01
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Abstract

Taurine has a neuroprotective action from oxidative stress in neural cell. In the present study, we studied taurine transport under basal and stressed conditions in conditionally immortalized rat brain capillary endothelial cell line (TR-BBB13) in vitro. The uptake of[$^3{H}$]taurine in the TR-BBB13 was increased by time-dependently and dependent on both Na$^{+}$ and Cl/ sup -/. Furthermore, $\beta$-alanine strongly inhibited the uptake of [TEX>$^3{H}$]taurine in the TR-BBB13. To study the effcts of oxidative stress on taurine transport, we used diethyl maleate (DEM) and lipopolysccharide (LPS). Diethyl maleate (DEM, $300\Mu\textrm{M}$) significantly reduced uptake of [TEX>$^3{H}$]taurine by time-dependently until 8 hr exposure in TR-BBB 13. But, the [TEX>$^3{H}$]taurine uptake was not changed by lipopolysccharide (LPS, 10 ng/ml) in TR-BBB13.3.

Keywords

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