• Nutritional Sciences
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• 제9권1호
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• pp.9-13
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• 2006

The differential effects of various fatty acids such as n-3 and n-6 types or degrees of unsaturation on the CYP2E1 induction and the production of lipid peroxidation (LPO) were investigated. The CYP2E1-transduced human hepatoma HepG2 cells (E47) were cultured in RPMI 1640 media containing different concentrations of various fatty acids up to 48 h incubation compared to 04 cells and CYP2E1-null cells. Treated fatty acids were linoleic acid (LA:n-6, C18:2), arachidonic acid (AA:n-6, C20:4) and docosahexaenoic acid (DHA:n-3, C22:6). The cell survival rate was decreased corresponding to the degree of unsaturation (LA>AA $\cong$DHA) and to LPO production in E47 and 04 cells. The four or five unsaturation degree of fatty acids, AA and DHA, caused time- and dose-dependent cell death in E47 cells but not as much as in C34 (without CYP2E1), suggesting an important role of CYP2E1 in the DHA mediated damage. In the levels of lipid peroxides (LPO), AA also elevated LPO by 3- and 5- fold compared to DHA or LA treated E47 cells. However, AA did not increase LPO until 48 h incubation in C34 cells. In conclusion, the polyunsaturated fatty acids induced CYP2E1 induction might be changed by the elevated levels of lipid peroxide (LPO) and oxidative stress through the connection of CYP2E1 and degrees of unsaturated fatty acids.

• 한국광과학회:학술대회논문집
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• 한국광과학회 2002년도 정기총회 및 제9회 광과학 학술심포지움 논문초록
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• pp.58-58
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• 2002

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.180.3-180
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• 2003

No Abstract, See Full Text

• Journal of Nutrition and Health
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• 제21권1호
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• pp.61-74
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• 1988

Ten college women were divided into 5 groups and treated in randomized block design for 5 weeks with 1 interval between treatments and subjects serving as their own controls. The experimental diets were corn oil diet as a source of n-6 linoleic acid, perilla oil diet as a source of n-3 $\alpha$-linolenic acid, and fish oil diet as a source of n-3 EPA and DHA. Dietary fat was supplied at 30% Cal and modified to give the total amount of saturated fatty acids and monoenoic acids at constant level. There was no significant effect on serum cholesterol level by different PUFA. However, on a gram-for-gram basis, there was a trend that the decrease in serum cholesterol was proportionate to the degree of fat unsaturation. On the other hand, only fish oil diet significantly decreased TG level but no significant effect on the relative proportion of TG in VLDL. The degree of hypotriglyceridemia did not corrleate with the degree of unsaturation. The relative proportion of CE in LDL was reduced by all PUFA diets but significant only by perilla oil diet. The relative amount of apoprotein in LDL was significantly reduced by n-3 PUFA. HDL-Chol content was significantly increased only in fish oil diet but no change in the relative proportion of its chemical components of HDL.

• Journal of Nutrition and Health
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• 제29권2호
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• pp.159-165
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• 1996

This study was performed to investigate the effects of concentration and degree of unsaturation of fatty acids on cellular proliferation and lipid peroxide production, using primary skin fibroblasts from neonate rats Fibroblasts (CPD : 2.8-5.4). Cells were cultured either in control medium (Dulbecco's modified Eagle's medium supplement with 10% fetal bovine serum) or in media supplemented with various kinds (stearic, oleic, linoleic, arachidonic, linolenic, eicosapentaenoic acid) and amounts (5, 10, 25, 50, 100, 150uM)of fatty acids. Cellular proliferation ratio and lipid peroxice production were measured and morphological changes were observed. Cellular proliferation was inhibited and morphological changes were observed. Cellular proliferation was inhibited and morphological changes were observed in cells grown in stearic containing media. Oleic, arachidonic, and eicosapentaenoic aicd tend to stimulate cellualar proliferation, and linolenic acid had no effects. Lipid peroxide concentrations in fibroblasts increased in proportion to the contents and unsaturation of fatty acids in media. Especially supplementation of arachidonic acid accelerated cellualr lipid peroxidation. Free radicals may cause severs damage to biological molecules, so lipid peroxidation probably contributes cellular membrane damages. However there were little relationship between lipid peroxide production and cellular proliferation in this study. (Korean J Nutrition 29(2) : 159~165, 1996)

• Ocean and Polar Research
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• 제29권1호
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• pp.1-8
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• 2007

A series of long chain unsaturated ketone (alkenone) was identified in the particulate organic matter sampled from surface water of the eastern South Sea, Korea. The seawater temperature based on the $C_{37}$ alkenone was calculated by using several different equations of unsaturation index(${U^{K'}}_{37}$), compared with in situ CTD temperature. Among the previously reported equations, the equation(0.044T-0.204) which was proposed by Sawada et al.(1996) seems to be the most useful for the calculation of $C_{37}$ alkenone temperature, showing average $0.51^{\circ}C$ difference between ${U^{K'}}_{37}$ temperature and in situ CTD temperature. This result suggest that alkenone should be a useful molecular biomarker for reconstructing paleo-environmental change in the South and East Sea, Korea. However, it is required to establish more reliable equation for the calculation of $C_{37}$ alkenone temperature.

• 미생물학회지
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• 제23권4호
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• pp.252-258
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• 1985

Aspergillus niger var. macrosporus, Aspergillus fumigatus, Penicillium notatum 및 Penicillium spinulosum의 곰팡이를 배양 온도 및 배양 기간을 달리하여 배양하고 이들 곰팡이가 생산한 지질량과 지질의 지방산 조성을 분석하였다. 이들 곰팡이에 의해 생성된 최대 지질량은 Aspergillus niger var. macrosporus가 17.8%. Aspergillus fumigatus가 31 %. Penicillium notatum이 12.6% 그리고 Pen. spinulosum이 17.5%였다. 이들 균주가 생산한 지질의 주요 지방산은 palmitic acid, stearic acid, oleic acid, linoleic acid였으며 Asp. niger var. macrosporus와 Asp. fumigatus는 전체 배양 기간 동안 oleic acid의 함량이 가장 높았으나 P. nota turn과 P. spinulosum은 linoleic acid의 함량이 가장 높았다. 그리고 지질의 불포화도는 Penicillium속이 Aspergillus속보다 높았다. 한편 지방산 조성은 배양온도에 따라 변화를 보였으나 대부분 일정한 경향을 나타내지 않았는데 Aspergillus fumigatus와 Penicillium notatum에서 저온 일수록 linoleic acid와 불포화도가 높아졌다.

• 한국작물학회지
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• 제36권5호
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• pp.429-444
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• 1991

수도품종의 내냉성 관련인자로서, 생체막 구성물질로서 중요한 역활을하는 phospholipid의 지방산조성과 지방산 불포화비율, 생체의 가장 중요한 대사과정의 하나인 호흡에 관련된 특성인 mitochondria의 호흡활성, 광합성된 엽록소 형광강도 등 여러가지 생리적 인자들과 내냉성과의 관계를 검토하고 내냉성 품종선발과 냉해를 경감시킬 수 있는 방법을 모색하고자 관련자료를 종합정리하였다. 1. 지방산의 불포화비율은 저온처리를 함으로서 높아지는 경향을 보였으며, 내냉성정도와 비교하여 볼 때 내냉성이 강한 품종에서 불포화비율이 높고 약한 품종에서 낮아 내냉성 정도와 일치하는 경향이었다. 2. 경화처리에 의하여 인지질의 지방산조성중 palmitic acid는 감소하고 linolenic acid의 함량은 증가되었으며 지방산 불포화비율이 대조구에 비하여 19～24% 증가되어 내냉성을 증가시킨 것으로 생각되었다. 3. 수도 백화묘($25^{\circ}C$ 암상태 2주간 생육) 및 야외에서 생육시킨 유묘에서 분리한 mitochondria의 호흡활성 전이온도와 내냉성 정도와의 관계를 보면 내냉성이 강한 품종에서 전이온도가 낮았고, 약한 품종에서는 높은 경향이었다. 4. 생육시기별 mitochondria의 호흡활성 전이온도는 상풍벼 11.5～12.5$^{\circ}C$, 풍산벼 15.0～l6.$0^{\circ}C$, 샛별벼 17.0～l8.$0^{\circ}C$로서 큰 차이가 없었으나 내냉성 강, 중, 약 정도간에는 뚜렷한 차이를 보였다. 5. 엽록소 형광강도와 내냉성 정도와의 관계는 내냉성이 강한 품종에서 형광강도가 높고 약한 품종에서 낮은 경향을 보였으며 높은 산관관계를 보였다. 6. Mn을 엽면시용하므로서 호흡활성 전이온도를 0～2$^{\circ}C$ 낮추었으며 토양처리를 하였을 때 내냉성이 약한 품종에서 효과가 켰다 7 내냉성 정도와 인지질 지방산불포화도, 호흡활성 전이온도 및 엽록소 형광과의 관계에서 대체적으로 이들 세 인자와는 높은 상관을 보였으나 그중에는 독특한 반응을 보인 품종들도 있었으며, 호흡활성 전이온도는 지방용 불포화비율과는 부의 관계를, 엽록소형광과 지방산 불포화비율과는 정의 상관관계를 보였다.

• 약학회지
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• 제25권4호
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• pp.181-186
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• 1981

Compositions of neutral lipid of the hazel nut (Corylus heterophylla Fischer var. thumbergii Blume, Betulaceae) produced in Korea were analyzed by thinchrography (Iatrosoan TH-10 type), which was equipped with a flame ionization detector. The neutral lipid components were nearly separated of the basis of their degree of unsaturation by employing the chromatography on silica gel sintered rod impregnated with 12.5% silver nitrate and it was noted that triglyceride was the major lipid components of Korean hazel nut. The triglyceride compositions were trilinolenin 10.63, trilinolein 18.01, triolein 42.24, tripalmitin 17.57 and tristearin 11.55% by thinchrographic analysis.

• Bulletin of the Korean Chemical Society
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• 제17권9호
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• pp.826-830
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• 1996

Fourier transform infrared (FTIR) temperature studies were performed to examine the microstructural changes that occur in annealing process of the thin films of 1,2-polybutadiene (1,2-PBu)/palladium chloride (PdCl2) complex. The disappearance of the infrared absorption intensities at 1640, 1418, 994 and 910 cm-1 signifies the consumption of 1,2-vinyl groups of 1,2-PBu. The progressive loss of unsaturation and production of methyl groups as a function of temperature were identified by the enhanced infrared absorption intensities at 1447 and 1375 cm-1. The loss of pendent carbon-carbon double bond is considered to involve both palladium-catalyzed addition reaction and thermally induced cyclization.