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Quantification of nitric oxide concentration in the hemocytes of Manila clam Ruditapes philippinarum by using 4,5-diaminofluorescein diacetate (DAF-2) detection method (바지락 (Ruditapes philippinarum) 혈구의 일산화질소 (nitric oxide) 정량)

  • Nam, Ki-Woong;Yang, Hyun-Sung;Park, Kyung-Il
    • The Korean Journal of Malacology
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    • v.29 no.1
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    • pp.15-21
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    • 2013
  • Nitric oxide (NO) is an important intra-intercellular signaling molecule that regulates many physiological processes and participates in the development some pathological conditions in animals. In this study, we compared different methods for determining NO concentration in the hemocytes of Manila clam Ruditapes philippinarum. For measuring the intracellular NO levels, we used the specific fluorescent probe 4,5-diaminofluorescein diacetate (DAF-2 DA), and the quantification methods that were compared were based on image analysis, spectrophotometry, and flow cytometry. NO concentration could be determined using all the 3 methods, and the concentration varied significantly depending upon the presence of NO regulators in the hemocytes; NO concentration increased in the presence of L-arginine, while it decreased in the presence of N-nitro-L-arginine methyl ester. In particular, it is found that estimation of NO using a flowcytometry is more economical, reliable and accurate compared to image analysis and spectrophotometry. Accordingly we believe that determining NO concentration by using flowcytometry will be useful in evaluating physiological and pathological conditions in marine bivalves.

Evaluation of Flow Experience by using Psychophysiological Visual Feedbacks

  • Kim, Jung Yong;Min, Seung Nam;Park, Yong Duck
    • Journal of the Ergonomics Society of Korea
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    • v.32 no.6
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    • pp.481-487
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    • 2013
  • Objective: The present study aims to evaluate the visual reactions of users when they are playing games of different flow levels, and to explore the visual variables that can sensitively reflect the different flow levels. Background: The flow is defined as a psychological state where interface users feel their actions in a virtual setting identical to those in real environment. To measure the flow states of users, the questionnaire-based FSS(Flow State Scale) has mostly been used. However, this method is a qualitative test that has limits in terms of the accuracy of users' flow experiences. Therefore, more accurate methods to measure users' flow experiences are required. Method: Ten subjects participated in the experiment, where the independent variables were three games with different flow levels(puzzle games, dot drawing and coloring) and the time frame(the first and last 10 seconds in game playing), whereas the dependent variables included the pupil size and the frequency and duration of eye blinking. This study was a within-subject design. Each participant performed three types of games with different flow levels 3 times for each for 10 minutes, and their visual reactions to each game were measured. Results: The higher the flow cause the bigger pupil size(p<0.01) and the lower eye blinking frequency(p<0.1), indicating that different types of games lead to different flow levels. The pupil size during the last 10 seconds when the flow level was higher was bigger by 2.1% compared with that during the first 10 seconds in game playing(p<0.1), and the eye blinking frequency decreased by 12%(p<0.01). Conclusion: It was found out that the pupil size and the frequency and duration of eye blinking were psychophysiological indices for evaluating users' flow experiences, which could quantify the flow states users go through. The psychophysiological variables capable of measuring diverse aspects of the flow need diversifying to be applicable to precise measurement of the flow. Application: These studies are warranted for both quantitative analysis of flow levels and qualitative improvement of cyber leisure in line with development of healthy games.

A Study on the Safety Handling Method of KCG's Water Jet Propulsion Ship (해양경찰 Water Jet 추진함정의 안전 조함법 연구)

  • Yun, Chong-Gum;Pak, Chae-Hong;Park, Deuk-Jin;Jung, Cho-Yeong
    • Journal of Navigation and Port Research
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    • v.41 no.6
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    • pp.373-380
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    • 2017
  • Operational errors caused by human factors, which is the major cause of marine accidents, include lack of knowledge, misunderstanding knowledge, and inadequate procedures. Recently, the type of propulsion mounted on KCG cutters has been diversified. In particular, the water jet propulsion unit, which was mainly installed in small boats, have been gradually expanded to medium and large size Coast Guard cutters, reaching 50% of the total. Axes types are divided into 2 to 4, and the bucket types are divided into Double Reverse Bucket(DRB) and Single Reverse Bucket(SRB); in these, the backward and steering control methods are completely different. Diversification of these operating systems can increase factors causing human error by the ships' operators. However, there is a lack of research on the maneuvering methods, considering the inherent active characteristics of each type of water jet. In this paper, we analyze the sideway method suitable for the condition of Coast Guard Exclusive wharf without assistance, based on the astern performance of each type. Then, a ship handling simulator was used for the experiment; they compared and verified through interviews of captains.

The emotional response that has an influence on achievement goal and ability to performance of senior citizen sport instructor (노인체육 지도자에 대한 참여자의 감정반응이 성취목표와 수행능력에 미치는 영향)

  • Park, Yeon-Hee;Choi, Hwan-suk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.5
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    • pp.186-194
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    • 2016
  • This study was conducted to determine whether if the emotional response of participants influences senior citizens at a senior citizen center and to develop the qualities and skills of the instructors of the senior citizens. Not only will we provide effective materials but also provide basic materials as well. The emotional response of interpersonal relationships, achievement goal of ability to performance, and influence of orientating themselves. Additionally, the emotional response of interpersonal relationships, ability to concentrate, and confidence. Finally, the achievement goal of task orientation, concentration of ability to performance on orientating oneself, as well as confidence. Therefore, we will be able to determine if the emotional response of a participant influences achievement of a goal and of the effectiveness of senior citizen instructors. This will not only help with practical and cultural issues, but will close the distance between the instructors participants based on consideration of their situational aspects. Furthermore, the sports activities and continued participation of the elderly. Always need to be ready mentally, and rather than the consideration of others as well as the game results, it is important to have an interest in achieving their own standards. Instructors toned to lend support while setting goals for the participants so that they contribute to the personal development and overall quality of life of the participants.

Dynamic response of nano-scale plates based on nonlocal elasticity theory (비국소 탄성 이론을 이용한 나노-스케일 판의 강제진동응답)

  • Kim, Jin-Kyu;Han, Sung-Cheon;Park, Weon-Tae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.1
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    • pp.436-444
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    • 2013
  • This article presents the dynamic response of nano-scale plates using the nonlocal continuum theory and higher-order shear deformation theory. The nonlocal elasticity of Eringen has ability to capture the small scale effects and the higher-order shear deformation theory has ability to capture the quadratic variation of shear strain and consequently shear stress through the plate thickness. The solutions of transient dynamic analysis of nano-scale plate are presented using these theories to illustrate the effect of nonlocal theory on dynamic response of the nano-scale plates. The relations between nonlocal and local theories are discussed by numerical results. Also, the effects of nonlocal parameters, aspect ratio, side-to-thickness ratio, size of nano-scale plate and time step on dynamic response are investigated and discussed. The amplitude and cycle increase when nonlocal parameter increase. In order to validate the present solutions, the reference solutions are used and discussed. The theoretical development as well as numerical solutions presented herein should serve as reference for nonlocal theories as applied to the transient dynamic analysis of nano-scale structures.

Design Methods for Eccentrically Loaded Bolt Groups for the Single Plate Connections Considering Sloped Edge Distance (편심전단을 받는 단일판접합부의 경사연단거리를 고려한 볼트군의 설계법)

  • Choi, Sun Kyu;Yoo, Jung Han;Park, Jai Woo
    • Journal of Korean Society of Steel Construction
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    • v.26 no.1
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    • pp.43-53
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    • 2014
  • A single plate connection(SPC) consists of a plate welded to the columns and bolts connected to the beam web. The SPC is widely used for a simple shear connection of steel structure because it is easy-to-fabricated, easy-to-installed and economical. The conventional SPC is used for 2 to 12 bolts in a single vertical row. It is designed to limit the plate thickness by bolt diameter to obtain flexible and ductile connections. The design strength for eccentric shear shall be the lesser of the shear strength of bolts or bearing strength of plate and when the design strength is decided by edge distance failure, the results can be very conservative. Although the research on special solution for 'weak-plate/strong-bolt' model with 2 to 4 bolts has been conducted by L. S. Muir, and W. A. Thonton, 2004, study on generalized design procedures did not conduct. This study proposed design procedure for evaluation of the design strength of eccentric shear bolt groups on a single plate connection based on the actual edge distance and the direction of bolt reaction forces by using elastic vector method(EVM) and instantaneous center of rotation method(ICM).

Integer Programming-based Operation Sequencing for Multi-operation on Single Machine (정수계획법을 통한 다중작업 수행 단일기계에서의 작업순서 결정)

  • Park, Seonyeong;Shin, Moonsoo
    • Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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    • v.9 no.3
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    • pp.261-270
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    • 2019
  • With the advent of the Fourth Industrial Revolution, multi-variety production in the up-to-date manufacturing environment is proceeding more rapidly whereby production planning and management have been getting more complicated. Moreover, the need to improve production efficiency through effective operation sequencing is further heightened. Fundamentally, the effective operation sequencing can reduce the set-up of the equipment, efficiently utilize the equipment, shorten the set-up time, and ultimately contribute to productivity improvement. This study deals with the problem of efficient operation sequencing in a situation where a single machine performs multiple operations. The complexity of the problem is very high when compared to the case where only one operation is performed on one machine, which is covered in most existing studies. In this paper, we propose an integer programming model to minimize the number of setups. This study aims at minimizing the number of mold replacement times in the process of processing a given production order for the wiring harness manufacturing process, which is one of the components of automobile electric field. In addition, brief case studies are presented to verify the validity of the proposed mathematical model.

A Study on the Factors Affecting the Period of Job Maintenance of Persons with Cerebral Palsy (뇌성마비인의 직업유지기간에 영향을 미치는 요인 연구)

  • Kim, Jae Ik;Park, Soon
    • 재활복지
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    • v.22 no.1
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    • pp.87-114
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    • 2018
  • The purpose of this study is to identify through empirical analysis about factors affecting the period of job maintenance of workers with cerebral palsy. Based on this, workers with cerebral palsy are looking for ways to maintain their job continuously. For this purpose, this study identifies the general characteristics and verify the affect of demographic factors, disability-related factors, employment-related factors, and social environment factors on the job maintenance period of workers with cerebral palsy. For the analysis, frequency analysis, descriptivel analysis, T-test and One-way Anova was conducted for 233 persons with cerebral palsy. The results of the study, of 17 variables of the four factors affecting the job maintenance period, 15 variables obtained statistically significant results. First, the demographic variables were age, household income, marital status, and education level. Second, the disability-related variables were disability grade, disability cause, and communication ability. Third, the employment-related variables were wage, employment type, disability-related organizations and companies, job type, job training, and qualification. Fourth, the social environment variables were assistive technology devices, and labor support personnel services.

Implementing an Adaptive Neuro-Fuzzy Model for Emotion Prediction Based on Heart Rate Variability(HRV) (심박변이도를 이용한 적응적 뉴로 퍼지 감정예측 모형에 관한 연구)

  • Park, Sung Soo;Lee, Kun Chang
    • Journal of Digital Convergence
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    • v.17 no.1
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    • pp.239-247
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    • 2019
  • An accurate prediction of emotion is a very important issue for the sake of patient-centered medical device development and emotion-related psychology fields. Although there have been many studies on emotion prediction, no studies have applied the heart rate variability and neuro-fuzzy approach to emotion prediction. We propose ANFEP(Adaptive Neuro Fuzzy System for Emotion Prediction) HRV. The ANFEP bases its core functions on an ANFIS(Adaptive Neuro-Fuzzy Inference System) which integrates neural networks with fuzzy systems as a vehicle for training predictive models. To prove the proposed model, 50 participants were invited to join the experiment and Heart rate variability was obtained and used to input the ANFEP model. The ANFEP model with STDRR and RMSSD as inputs and two membership functions per input variable showed the best results. The result out of applying the ANFEP to the HRV metrics proved to be significantly robust when compared with benchmarking methods like linear regression, support vector regression, neural network, and random forest. The results show that reliable prediction of emotion is possible with less input and it is necessary to develop a more accurate and reliable emotion recognition system.

Fabrication and Evaluation of Mercury(II) Iodide Unit Cell Dosimeter to Confirm the Feasibility of Digital Quality Assurance in Intracavitary Radiotherapy (방사선 근접치료의 디지털 정도관리 가능성 확인을 위한 HgI2 Unit Cell 선량계의 제작 및 평가)

  • Jung, Jae-Hoon;Park, Sung-Kwang
    • Journal of the Korean Society of Radiology
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    • v.16 no.3
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    • pp.257-263
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    • 2022
  • In intracavitary radiotherapy, it is essential to verify the correct location of radiation source among quality control items because an incorrect location will irradiate an unnecessary dose to normal tissues. As a basic study of digital line dosimeters, this study fabricated a unit cell dosimeter based on polycrystalline mercury (II) iodide (HgI2) and compared its performance with a diode. The study result showed that for reproducibility, the relative standard deviation (RSD) was 1.21%, satisfying the RSD evaluation criterion of within 1.5%. Considering linearity, the coefficient of determination R2 showed an excellent result of 0.9997. Regarding the evaluation of distance dependence, it showed a similar trend in general with a difference of 0.035 cm for intensity 50% when compared with the inverse square value. This study suggests the applicability of a digital dosimeter for brachytherapy quality control by evaluating the performance of the HgI2 dosimeter. This study on dosimeter for candidate photoconductor materials can be used as basic data in all areas using radiation.