• 제목/요약/키워드: university lab safety

검색결과 312건 처리시간 0.021초

Lactic Acid Bacteria from Gamecock and Goat Originating from Phitsanulok, Thailand: Isolation, Identification, Technological Properties and Probiotic Potential

  • Hwanhlem, Noraphat;Salaipeth, Lakha;Charoensook, Rangsun;Kanjan, Pochanart;Maneerat, Suppasil
    • Journal of Microbiology and Biotechnology
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    • 제32권3호
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    • pp.355-364
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    • 2022
  • From independent swab samples of the cloaca of indigenous gamecocks (CIG), anus of healthy baby goats (AHG), and vagina of goats (VG) originating from Phitsanulok, Thailand, a total of 263 isolates of lactic acid bacteria (LAB) were collected. Only three isolates, designated C707, G502, and V202, isolated from CIG, AHG, and VG, respectively, exhibited an excellent inhibitory zone diameter against foodborne pathogenic bacteria when evaluated by agar spot test. Isolates C707 and G502 were identified as Enterococcus faecium, whereas V202 was identified as Pediococcus acidilactici, based on 16S rRNA sequence analysis. When foodborne pathogenic bacteria were co-cultured with chosen LAB in mixed BHI-MRS broth at 39℃, their growth was suppressed. These LAB were found to be capable of surviving in simulated stomach conditions. Only the isolate G502 was able to survive in the conditions of simulated intestinal juice. This research suggests that selected LAB could be used as a food/feed supplement to reduce foodborne pathogenic bacteria and improve the safety of animal-based food or feed.

치과기공사의 안전교육 실태 및 상해발생 조사연구 -서울·경기 지역을 중심으로 (Study of the safety education condition and injury occurrence in dental technicians- in Seoul and Gyeonggi)

  • 김진;최세웅;신수정;조윤상;임수민;심정석;이정수;배은정
    • 대한치과기공학회지
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    • 제39권3호
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    • pp.179-185
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    • 2017
  • Purpose: It is intended to raise awareness of importance of protective equipment and necessity of safety education by examining the actual condition of measures to deal with accident, occurrence of injury, whether safety education is received or not, the actual condition of wearing protective equipment, and working environment of dental lab and dental technician. Methods: A self-administered survey was conducted for a total of 150 dental technologists. A chi-squared test and independent-samples t-test was carried out by using SPSS WIN Program (version 12.0) in order to analyze the actual condition of safety education according to work experience and position and whether injury occurred according to whether to wear protective equipment. Significance level was 0.05. Results: With regard to whether injury occurred according to whether safety education was received, there was 'receiving the safety education-injured (71.8%)', 'not receiving the safety education-injured (79.7%)', 'receiving the safety education-not injured (28.2%)', and 'not receiving the safety education-not injured (20.3%)'. The statistical results of injury occurrence according to safety education were not significant. With regard to whether to be injured according work experience, there was 'less than 10 years-injured (68.4%)', 'less than 10 years- not injured (31.6%)', '10 years or above-injured (89.1%)', and '10 years or above-not injured (10.9%)'. The statistical results of injury occurrence according to work experience were significant (p<0.001). Conclusion: Dental lab and specialized agencies should continue to be interested in periodic safety training, recommending wearing protective equipment and personal protective equipment support and so on.

마우스에서 황토국화 추출물의 급성독성 연구 (Acute Toxicity Study on Mud chrysanthemum Indicum in Mice)

  • 마진열;박화용;최한;지옥표;이재훈
    • 대한본초학회지
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    • 제23권2호
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    • pp.81-85
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    • 2008
  • Objectives : The aim of this study is to collect data for toxicity and safety of Mud chrysanthemum indicum. Methods : In this study, we investigated the acute toxicity for buthanol-extracted Mud schrysanthemum indicum, 25 male and 25 female mice were observed for 14days after one day oral administration of Mud chrysanthemum indicum at the respective doses of 0(control group), 1024, 1280, 1600 and 2000mg/kg. Results : We observed survival rates, general toxicity, change of body weight and autopsy. No abnormality was found for all cases. Conclusions : The data confirmed that Mud chrysanthemum indicum is free from, the toxicity and safety problems. Compared with the control group, we could not find any toxic alteration in all treated groups (1024, 1280, 1600 and 2000mg/kg), In conclusion, LD50 of Mud chrysanthemum indicum was over 2000 mg/kg and it is very safe to mice.

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4M 및 HAZOP 기법을 활용한 연구실 위험요소 분석 연구 (A Study on the Hazard Identification of Laboratory using 4M & HAZOP)

  • 김태훈;이광원;서두현;이익모;윤충식;이윤근;박정임
    • 한국안전학회지
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    • 제28권3호
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    • pp.88-94
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    • 2013
  • In university laboratories, areas of studies are becoming diverse and complicated according to the development of the industry. New forms of potential risk factors are increasing and they are unlike existing ones. In addition, many students are conducting various experiments in the laboratory. Therefore, they could be exposed to risk more often. Despite these risks, people do not recognize university lab safety activities properly and observe safety precautions. They are exposed to various laboratory accidents continually. In this study, we do not apply the present diagnosis method, checklist, but the safety assessment that is widely used in industry. Then we can find lots of hazard that checklist method could miss. This study will use the 4M and Hazard & Operability to design a new Laboratory safety assessments method.

NI-9223과 전류프로브를 이용한 전류 및 누설전류 측정장치 개발 (Development of Electric Instrument of Current and Leakage Current based on NI-9223 and Current Prove)

  • 김성철;김운술
    • 한국안전학회지
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    • 제27권6호
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    • pp.48-53
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    • 2012
  • This paper is purposed to develop portable electric instrument to select NI-9223(National instrument comp.) and clamp meter(HIOKI comp.), which can be used in developing electric instrument, to detect leakage current(ZCT) and current(CT) signals. In this paper, The electric instrument that can interface with current and leakage current instrument(HIOKI 9283), is developed by NI-9223 of NI comp.. HIOKI clamp meter can measure current signals certainly by high-sensitivity of 10 ${\mu}A$ resolution(leakage current : at 10 mA range) and current 1~200A range. The NI-9223 use four 16-bit analog-to-digital converters(ADCs) for true simultaneous sampling at up to 1 MS/s per channel. NI-9223 can synchronize all analog input modules installed in the same chassis to share the same start clock and/or sample clocks. The monitoring program is developed by SignalExpress of LabVIEW. The monitoring program are developed to analyze at simultaneous sampling on electrical signals such as leakage current(ZCT) and current(CT). The developed system verification tests were conducted, and portable electric instrument can be used in place which requires analysis of the actual electrical signal.

고등학생들의 연구실 이용에 따른 안전 인식 및 교육 필요성에 관한 연구 (A study on necessity of education and safety awareness to high school students when accessing laboratories)

  • 최서연;이하연
    • 한국산학기술학회논문지
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    • 제17권10호
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    • pp.139-146
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    • 2016
  • 본 연구는 고등학생의 연구실 이용에 따른 안전 인식과 교육에 대한 필요성을 조사하였다. 이를 위하여 서울 소재 3개 고등학교 2학년 재학생 290명을 대상으로 설문 조사를 실시하였으며, 수집된 자료를 분석하였다. 분석 결과 정규 수업 전 안전교육은 64.8%가 경험한 것으로 나타났으며, 사고 경험 6.6%, 체험 교육 시 안전 교육 68.0%로 사전 안전교육이 부족한 것으로 나타났으며, 안전교육에 대한 인식도와 필요성은 높게 인식하고 있는 파악되었다. 특히, 연구실 사고 대처 방법에 관한 교육의 필요성을 안전 교육 경험 학생이 높게 나타나 연구실 안전 교육의 체계적인 관리 및 학생들의 안전 시 사고체계에 대한 다각적인 관리 방안 마련이 요구된다. 연구실 안전 인식과 교육 필요성 요인은 생활안전과 연구실 이용 안전교육 필요성과 상호 관련성이 높았고, 기기 및 실험 안전에 대한 필요성이 높을수록 연구실 이용 안전과 사고 대처 방법에 대한 인식도 높은 것으로 나타나 고등학생 대상의 연구실 안전 교육 프로그램 개발과 관리적 방안에 관한 연구를 제안한다.

Thermal-hydraulic research on rod bundle in the LBE fast reactor with grid spacer

  • Liu, Jie;Song, Ping;Zhang, Dalin;Wang, Shibao;Lin, Chao;Liu, Yapeng;Zhou, Lei;Wang, Chenglong;Tian, Wenxi;Qiu, Suizheng;Su, G.H.
    • Nuclear Engineering and Technology
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    • 제54권7호
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    • pp.2728-2735
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    • 2022
  • The research on the flow and heat transfer characteristics of lead bismuth(LBE) is significant for the thermal-hydraulic calculation, safety analysis and practical application of lead-based fast reactors(LFR). In this paper, a new CFD model is proposed to solve the thermal-hydraulic analysis of LBE. The model includes two parts: turbulent model and turbulent Prandtl, which are the important factors for LBE. In order to find the best model, the experiment data and design of 19-pin hexagonal rod bundle with spacer grid, undertaken at the Karlsruhe Liquid Metal Laboratory (KALLA) are used for CFD calculation. Furthermore, the turbulent model includes SST k - 𝜔 and k - 𝜀; the turbulent Prandtl includes Cheng-Tak and constant (Prt =1.5,2.0,2.5,3.0). Among them, the combination between SST k - 𝜔 and Cheng-Tak is more suitable for the experiment. But in the low Pe region, the deviation between the experiment data and CFD result is too much. The reason may be the inlet-effect and when Pe is in a low level, the number of molecular thermal diffusion occupies an absolute advantage, and the buoyancy will enhance. In order to test and verify versatility of the model, the NCCL performed by the Nuclear Thermal-hydraulic Laboratory (Nuthel) of Xi'an Jiao tong University is used for CFD to calculate. This paper provides two verification examples for the new universal model.

실험적 연구를 통한 퇴적암의 한계변형률 특성에 관한 연구 (Experiments Study on Critical Strain Properties of Sedimentary Rocks)

  • 이재호;김영수;김광일;박장호;박시현
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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    • pp.211-219
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    • 2008
  • The hazard warning levels are necessary for the rational design and safety construction of underground space, as mountain and urban tunnel. Sakurai provided the hazard warning levels for assessing the stability of tunnels using the critical strain of rock mass, which is defined as a ratio between uni-axial compressive strength and the Young's modulus. The concept of critical strain guidelines is introduced in this study for the assessment of tunnel safety during excavation. Moreover, in this paper, the critical strain properties of sedimentary rock in Korea has investigated and analysed in detail by Lab. test, as the uniaxial compression tests. Finally, critical strain properties of sedimentary rock is discussed the relationship of failure strain values, uniaxial compression strengths and Young's modulus.

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노후 PSC 구조물의 잔여 긴장 응력 진단을 위한 외부 자화 EM 기법 검증 (Verification of External Magnetization based EM Technique for Diagnosing Residual Tensile Stress in Aged PSC Structures)

  • 박순전;박세환;최재훈;전교영;김준경
    • 한국전산구조공학회논문집
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    • 제36권4호
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    • pp.251-257
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    • 2023
  • 본 연구는 현재 가설되어 가용 중인 프리스트레스트 구조물에 대해서 긴장 응력을 계측하는 방법에 관한 연구를 위해 외부 자화를 이용한 PSC 텐던의 긴장 응력 계측에 관한 연구를 진행하였다. 이에 유한요소해석을 이용하여 PSC 거더에 외부 자화 시 잔존 긴장 응력을 검출하기 위해 PSC 거더 내부의 PS 텐던까지 영향을 줄 수 있는 코일 배치 및 크기 등을 고려하여 최적의 센서를 설계하였다. 또한, 유한요소해석을 이용하여 설계한 센서와 동일한 수치 및 재질 데이터를 이용해 이론적 검증을 진행하였으며 타겟 위치에서 자화의 세기를 계산하였을 때, 유한요소해석 결과와 동일한 결과를 얻어낼 수 있었다. 이를 통해 설계한 센서의 검증 및 비 접촉 외부 자화 EM 센서를 활용하여 PSC I형 거더 내부 텐던의 자화가 가능함을 확인하였다.

주거용 컨테이너 화재시 발생되는 연소가스의 독성 평가에 관한 연구 - 화재용 방독면 filter의 성능평가를 중심으로 (A Study on Toxicity Evaluation of Combustion Gases Released from the Residental Container Fire - Efficiency Test for the Fire Gas Mask Filters)

  • 이정윤;김정훈;김연희;정기창
    • 한국안전학회지
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    • 제19권4호
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    • pp.48-54
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    • 2004
  • The recent Ire incident in an elementary school of Chonan city causes the media focus on the fire safety of residential container buildings. In this study, real fire tests were conducted in this kind of buildings. Combustion products including $O_2,\; CO_2,\;CO,\;NOx,\;SOx,\;HCI,\;HCN$ were measured, and blood samples of lab rats were analyzed in terms of Co-Hb, Glucose, AST(GOT), ALT(GPT), in order to investigate the hazard-reduction effects of employing gas mask protected with filter during the fire emergency of residential container buildings. According to the test results, whether or not employing the filter showed a sheer difference in the toxicity of the fire-induced gases, and then the importance of wearing a gas mask was evidently demonstrated.