• Molecules and Cells
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• v.26 no.5
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• pp.443-453
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• 2008

The Bric-a-brac, Tramtrack, Broad-complex (BTB) domain is a protein-protein interaction domain that is found in many zinc finger transcription factors. BTB containing proteins play important roles in a variety of cellular functions including regulation of transcription, regulation of the cytoskeleton, protein ubiquitination, angiogenesis, and apoptosis. Here, we report the cloning and characterization of a novel human gene, KLHL31, from a human embryonic heart cDNA library. The cDNA of KLHL31 is 5743 bp long, encoding a protein product of 634 amino acids containing a BTB domain. The protein is highly conserved across different species. Western blot analysis indicates that the KLHL31 protein is abundantly expressed in both embryonic skeletal and heart tissue. In COS-7 cells, KLHL31 proteins are localized to both the nucleus and the cytoplasm. In primary cultures of nascent mouse cardiomyocytes, the majority of endogenous KLHL31 proteins are localized to the cytoplasm. KLHL31 acts as a transcription repressor when fused to GAL4 DNA-binding domain and deletion analysis indicates that the BTB domain is the main region responsible for this repression. Overexpression of KLHL31 in COS-7 cells inhibits the transcriptional activities of both the TPA-response element (TRE) and serum response element (SRE). KLHL31 also significantly reduces JNK activation leading to decreased phosphorylation and protein levels of the JNK target c-Jun in both COS-7 and Hela cells. These results suggest that KLHL31 protein may act as a new transcriptional repressor in MAPK/JNK signaling pathway to regulate cellular functions.

• BMB Reports
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• v.45 no.3
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• pp.153-158
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• 2012

Geft is a guanine nucleotide exchange factor, which can specifically activate Rho family of small GTPase by catalyzing the exchange of bound GDP for GTP. Geft is highly expressed in the excitable tissue as heart and skeletal muscle and plays important roles in many cellular processes, such as cell proliferation, migration, and cell fate decision. However, the in vivo role of Geft remains unknown. Here, we generated a Geft conditional knockout mouse by flanking exons 5-17 of Geft with loxP sites. Cre-mediated deletion of the Geft gene in heart using Mef2c-Cre transgenic mice resulted in a dramatic decrease of Geft expression. Geft knockout mice develop normally and exhibit no discernable phenotype, suggesting Geft is dispensable for the development of the second heart field in mouse. The Geft conditional knockout mouse will be a valuable genetic tool for uncovering the in vivo roles of Geft during development and in adult homeostasis.

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.242.1-242.1
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• 2001

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2007.05a
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• pp.157-157
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• 2007

• International Journal of Computer Science & Network Security
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• v.22 no.1
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• pp.7-14
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• 2022

The presented research is devoted to the problems of achieving the goals of sustainable development, which will contribute to the development of industrial parks and their transformation into eco-industrial parks. The importance of the functioning of industrial parks and their transformation into eco-industrial ones in the direction of achieving sustainable development goals is substantiated. The development of industrial parks and their transformation through the coordination of dynamic development processes and the establishment of a system of mutually beneficial relations, due to the peculiarities of their functioning aimed at achieving sustainable development goals ensure the achievement of sustainable spatial development. Transformation of industrial parks will contribute to: increasing tax revenues of local budgets, increasing employment in the regions, raising public awareness of the need for environmentally friendly, energy efficient production, increasing the balance and harmony of social, economic and environmental development, modernization of territorial communities and regional enterprises resources and increase entrepreneurial initiative, rational use of available resources, intensification of small and medium enterprises, including innovation. The authors' scientific achievements are the substantiation of the principles of functioning of industrial parks in order to achieve the goals of sustainable development, which are based on a systematic approach and provide for the transformation into eco-industrial parks.

• Proceedings of the Korean Nuclear Society Conference
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• 2002.05a
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• pp.63-63
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• 2002

• International Journal of Computer Science & Network Security
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• v.21 no.12
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• pp.269-279
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• 2021

In the current conditions of world socio-economic development, the strategic support of the process of managing the development of universities has become a particularly important area. Strategic management requires reliable information and analytical support in the form of sound descriptions of strategic directions of development, assumptions, and forecasts. The purpose of the study is to substantiate and elaborate the crucial causes in the strategic management of university's development and to suggest the coherent prospects for advancements. The data analysis was performed using descriptive methods to identify the most significant causes that affect the university's strategic development; the expert assessment was used to rank the factors, ultimately to assess each factor that affects to some extent the university's strategic development; the abstract-logical method was used to ground the positive impact of computer technologies and e-learning on the strategic development of a university and to formulate proposals for its further progress. The main results provided in the given paper showed that significant and most important strategic cause of university's development lies in the field of improving the quality of education, expanding access to educational services based on computer technology and its functionality. In turn, its widespread use at all stages of the educational process allows providing a number of advancements for universities in strategic prospects.

• Clinical and Experimental Pediatrics
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• v.55 no.11
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• pp.414-419
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• 2012

Purpose: This study aimed to assess early development in very low birth weight (VLBW) infants with mild intraventricular hemorrhage (IVH) or those without IVH and to identify the perinatal morbidities affecting early neurodevelopmental outcome. Methods: Bayley Scales of Infant Development-II was used for assessing neurological development in 49 infants with a birth weight <1,500 g and with low grade IVH (${\leq}$grade II) or those without IVH at a corrected age of 12 months. Results: Among the 49 infants, 19 infants (38.8%) showed normal development and 14 (28.6%) showed abnormal mental and psychomotor development. Infants with abnormal mental development (n=14) were mostly male and had a longer hospitalization, a higher prevalence of patent ductus arteriosus (PDA) and bronchopulmonary dysplasia (BPD), and were under more frequent postnatal systemic steroid treatment compared with infants with normal mental development (n=35, P<0.05). Infants with abnormal psychomotor development (n=29) had a longer hospitalization and more associated PDA compared to infants with normal psychomotor development (n=20, P<0.05). Infants with abnormal mental and psychomotor development were mostly male and had a longer hospitalization and a higher prevalence of PDA and BPD compared to infants with normal mental and psychomotor development (n=19, P<0.05). Using multiple logistic regression analysis, a longer duration of hospitalization and male gender were found to be significant risk factors. Conclusion: Approximately 62% of VLBW infants with low grade IVH or those without IVH had impaired early development.

• Architectural research
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• v.9 no.1
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• pp.19-30
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• 2007

This study explores the issue of campus expansion of an urban university using an in-depth case study of New York University in New York City, USA. It investigates the physical pattern and development mechanism of the campus expansion process on a city grid structure. The three elements that characterize NYU's recent campus expansion, are (1) the university's commitment to building a student community around a public open space on a subway network, (2) four types of property acquisition and building development practiced by the university with private developers for space leasing, building renovation and development under changing local development circumstances, and (3) the city's initiative for attracting the private sector whose development activities influence the university's development dynamics. These findings suggest that the expansion planning of an urban university, due to its location in a city, should be placed on a local planning agenda, which can positively contribute to public goals through the collaborative engagement of the private and public sectors.

• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.218.2-218.2
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• 2002