• Title/Summary/Keyword: university constituents

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Chemical Constituents of Helichrysum conglobatum Growing in Egypt

  • El-Ghazooly, Maged G.;El-Lakany, Abdalla M.;Abou-Shoer, Mohamed I.;Aly, Amal H.
    • Natural Product Sciences
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    • v.9 no.4
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    • pp.213-219
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    • 2003
  • Five aromatic compounds, of which two are new glucosides, and six flavonols were isolated and identified for the first time from the flower heads and aerial shoots of Helichrysum conglobatum (Asteraceae). Their structures were established on the basis of chemical and spectroscopic methods including UV, MS, 1D- and 2D-NMR. Some fractions and isolates were screened for anti-microbial activities. This is the first report of the isolation of the chemical constituents of this species.

Studies on the Biochemical Nature of the Protein Constituents of Panax Ginseng Root (인삼(人蔘) 단백성분의 생화학적성질(生化學的性質)에 관한 연구)

  • Kim, Young-Choong;Chung, Bo-Sup;Lee, Kang-No;Koo, Hyang-Ja;Ahn, Sang-Mee;Huh, Hoon
    • Journal of Nutrition and Health
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    • v.16 no.2
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    • pp.115-124
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    • 1983
  • The biochemical nature of the protein constituents of six year old fresh Panax ginseng root was studied. Total protein constituents were extracted with phosphate buffer of pH 7.4, ionic strength of 0.1 and fractionated by ultrafiltration using four different membranes which cut down the materials of molecular weight of 500, 1,000, 5,000 and 10,000, respectively. Each fraction was subjected to ion exchange chromatography using DEAE - cellulose to isolate component proteins. The protein fraction larger than molecular weight of 10,000 was refractionated by the method of ammonium sulfate precipitation. The electrophoresis of the refractionated protein constituents was performed. The amino acid composition of the protein constituents was determined by gas- liquid chromatography. From the results, it could be summarized that eleven different protein constituents smaller than molecular weight of 10,000 were isolated from the fresh Panax ginseng root. At least eleven different protein constituents larger than molecular weight of 10,000 were identified from the electrophoretic patterns. These protein costituents seem to be compounded of all or some of five different subunits.

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Research on the Evaluation of Communication Activities Space in City Park

  • Lv, Hong;Cho, Tae-Dong;Piao, Yong-Ji
    • Journal of Environmental Science International
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    • v.22 no.4
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    • pp.397-406
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    • 2013
  • With the methodology of AHP, this paper focuses on communication activities and their constituents in city parks, selecting 11 constituents, natural or artificial, and establishes an evaluation model on the basis of the analysis of the characteristics of tourists' communication activities in order to obtain the weight and order of importance of the constituents of communication activities space. First-grade indicators influence weight and the order of the constituents of communication activities space are: artificial constituents (0.6614) > natural constituents (0.3386). The weight and order of five secondary indicators attached to natural constituents: private space (0.1538) > shade tree (0.0955) > gentle slope mound (0.0474) > beautiful waterscape (0.0270) > sunshine lawn (0.0149); the weight and order of six secondary indicators attached to artificial constituents: field boundary (0.2865) > Leisure chairs (0.1843) > resting areas (0.0795) > appropriate square (0.0533) > tree-lined road (0.0352) > landscape sketch (0.0227).Using modern decision analysis methodology to research the relationship of environment constituent elements has great theoretical and practical significance for the scientific design and construction of suitable environment for human needs.

Studies on the Effect of the Protein Constituents of Panax ginseng Root on Cultured Chick Embryonic Brain, Spinal Cord and Skeletal Muscle Cells (인삼 단백성분이 배양한 Chick Embryo의 뇌, 척수, 근육세포에 미치는 효과에 관한 연구)

  • Kim, Young-Choong;Han, Dae-Suk;Huh, Hoon;Ahn, Sang-Mee;Koo, Hyang-Ja
    • YAKHAK HOEJI
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    • v.27 no.2
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    • pp.109-116
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    • 1983
  • The effect of protein constituents of six-year old fresh Panax ginseng root on chick embryonic brain, spinal cord and skeletal muscle dissociation cultures was studied. The protein constituents showed the enhancing effect on cultured brain, spinal cord and skeletal muscle cells. The neurite formation from brain and spinal cord cells and the outgrowth of neurite seemed to be enhanced by almost all of the protein constituents employed for this study. The maturation of skeletal muscle cells was stimulated by the protein constituents. This enhancing effect of the protein constituents was more vivid when brain, spinal cord and skeletal muscle cells were cultured with a medium which did not contain chick embryonic extracts known as an essential component for primary cell culture. The protein fraction having molecular weight range of 1,000 to 5,000 out of all the protein fractions employed for this study showed the most stimulatory effect on cultured brain, spinal cord and skeletal muscle cells.

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Studies on the Terpenoids in the Volatile Constituents of Liaoning Schisandra Chinensis Baillon

  • Hou, Dongyan;Zhang, Weihua;Hui, Ruihua
    • Analytical Science and Technology
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    • v.8 no.4
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    • pp.505-509
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    • 1995
  • The terpenoids in the volatile constituents of Liaoning Shcisandra Chinesis Baillon have been determined by the analytical method of GC/MS. Thirty terpenoids molecular structure were characterized. They are 11.89% monterpenes, 4.60% monoterpene oxides, 58.74% sesquiterpene hydrocarbons, and 1.62% oxygen-containing sesquiterpenoids in the total volatile constituents quantified by chromatograph. Among them, the sesquiterpene make up the characteristic constituents. Every terpenoid constituent percent content was obtained using area normalization method of HP-59970 chemstation.

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Polyamine Constituents of Korea White Ginseng and Fresh Ginseng Root (한국산(韓國産) 백삼(白蔘)과 수삼(水蔘)의 Polyamine 성분에 관한 연구)

  • Kim, Young-Choong;Han, Dae-Suk;Cho, Hyun-Soo;Ahn, Sang-Mee;Huh, Hoon;Koo, Hyang-Ja
    • Korean Journal of Pharmacognosy
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    • v.14 no.2
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    • pp.39-43
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    • 1983
  • Polyamine constituents of fresh Panax ginseng root and dried white ginseng were extracted with 5% trichloroacetic acid, respectively. The isolation of polyamine constituents was conducted by the cation exchange chromatography using $Dowex-50W{\times}8$ resin and the detection was performed with TLC. Identification of the polyamine was carried out by the methods of IR, NMR, MS spectroscopy and GLC. Polyamine constituents of white and fresh ginseng root were identical and composed of five different polyamines. The major polyamine in white and fresh ginseng root was determined as putrescine.

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The Protective Effects of Phenolic Constituents from Gastrodia elata on the Cytotoxicity Induced by KCI and Glutamate

  • Huang, Zhan-Bo;Wu, Zhe;Chen, Fa-Kui;Zou, Li-Bo
    • Archives of Pharmacal Research
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    • v.29 no.11
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    • pp.963-968
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    • 2006
  • Seven phenolic compounds (1-7) were isolated from the tubers of Gastrodia elata. Their structures were elucidated on the basis of MS and NMR spectral data. p-Ethoxymethyl phenyl-O-${\beta}$-D-glucoside (1) was proved to be a new compound, with N-(p-hydroxybenzyl)-adenosine (7) isolated from this plant for the first time. In this study, the protective effects of the six constituents (1-6) on PC12 cells against the cytotoxicity induced by KCI and glutamate were also investigated. The viability of the PC12 cells was significantly enhanced by pretreatment with the six phenolic constituents.

Correlation Between M-A Constituents and Tensile Properties in the Intercritical Coarse Grained HAZ of an Ultra Low Carbon Steel (극 저탄소강의 Intercritical coarse grained HAZ에서의 M-A상과 인장특성 간의 상관관계)

  • Lee, Yoon-Ki;Moon, Joon-Oh;Kim, Sang-Hoon;Lee, Chang-Hee
    • Journal of Welding and Joining
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    • v.28 no.3
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    • pp.99-103
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    • 2010
  • It is well known that martensite-austenite (M-A) constituents are formed in the intercritically reheated coarse grained heat affected zone (ICCGHAZ) of a multipass weld and they act on the local brittle zone (LBZ) in the welded structures. To investigate the effect of M-A constituents on the tensile properties of ICCGHAZ, specimens with M-A constituents of different volume fraction and size were prepared through the multipass welding cycles simulated by a Gleeble simulator and then tensile test was carried out. The results indicated that finely distributed M-A constituents contributed to decrease the yield ratio, which is mainly due to the increased tensile strength.

Identification of Neuroactive Constituents of the Ethyl Acetate Fraction from Cyperi Rhizoma Using Bioactivity-Guided Fractionation

  • Sim, Yeomoon;Choi, Jin Gyu;Gu, Pil Sung;Ryu, Byeol;Kim, Jeong Hee;Kang, Insug;Jang, Dae Sik;Oh, Myung Sook
    • Biomolecules & Therapeutics
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    • v.24 no.4
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    • pp.438-445
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    • 2016
  • Cyperi Rhizoma (CR), the rhizome of Cyperus rotundus L., exhibits neuroprotective effects in in vitro and in vivo models of neuronal diseases. Nevertheless, no study has aimed at finding the neuroactive constituent(s) of CR. In this study, we identified active compounds in a CR extract (CRE) using bioactivity-guided fractionation. We first compared the anti-oxidative and neuroprotective activities of four fractions and the CRE total extract. Only the ethyl acetate (EA) fraction revealed strong activity, and further isolation from the bioactive EA fraction yielded nine constituents: scirpusin A (1), scirpusin B (2), luteolin (3), 6'-acetyl-3,6-diferuloylsucrose (4), 4',6' diacetyl-3,6-diferuloylsucrose (5), p-coumaric acid (6), ferulic acid (7), pinellic acid (8), and fulgidic acid (9). The activities of constituents 1-9 were assessed in terms of anti-oxidative, neuroprotective, anti-inflammatory, and anti-amyloid-${\beta}$ activities. Constituents 1, 2, and 3 exhibited strong activities; constituents 1 and 2 were characterized for the first time in this study. These results provide evidence for the value of CRE as a source of multi-functional neuroprotectants, and constituents 1 and 2 may represent new candidates for further development in therapeutic use against neurodegenerative diseases.