• The Journal of the Convergence on Culture Technology
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• v.9 no.3
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• pp.271-279
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• 2023

The purpose of this study was to investigate the effect of learning flow, problem solving ability, professor-student interaction of academic achievement in nursing students. Data were collected from 274 nursing students in B city and analyzed by t-test, ANOVA, Pearson correlation coefficient, and hierarchial multiple regression using SPSS/WIN 22.0. The degree of academic achievement in nursing students was 3.70±0.70. There were significant differences in academic achievement with grade(F=4.755, p=.003), campus life satisfaction(F=5.643, p=.004), major satisfaction(t=5.794, p=.003), adapting to COVID-19(F=7.961, p<.001), satisfaction to non-face-to-face environment class(F=18.353, p<.001). There was positive correlation between academic achievement and learning flow(r=.649, p<.001), problem solving ability(r=.333, p<.001), professor-student interaction(r=.479, p<.001). The factors affecting academic achievement of the study subjects were learning flow(β=.563, p<.001), professor-student interaction(β=.280, p<.001), with an explanatory power of 52.0%. Therefore, strategies increase the academic achievement of nursing students in untact lecture, and environment improvement to increase learning flow and professor-student interaction are needed.

• Journal of Plant Biotechnology
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• v.50
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• pp.63-69
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• 2023

This study includes the transformation of genes such as ORE7, the increase of gene expression, and the use of the bar gene as a selectable marker that shows herbicide resistance with Agrobacterium tumefaciens using hypocotyls from the oilseed rape "Youngsan" cultivar. To establish an Agrobacterium transformation system for the production of oilseed rape with a high-yield trait, infection time and co-cultivation period with Agrobacterium were tested. Therefore, when hypocotyls from the oilseed rape "Youngsan" cultivar were infected with Agrobacterium for 20 min and co-cultivated for 3 days, approximately 32-36 putatively transformed hypocotyls with shoots including roots survived from 100 inoculated hypocotyls after 4 weeks of transformation on a selection medium containing 20 mg/L of phosphinothricin (PPT) as a selectable agent. Additionally, a PCR assay was performed to confirm the insertion of target genes and showed the presence of the ORE7 gene as a high-yielding trait and the bar gene as a selectable marker. Treatment with 0.5% (v/v) Basta solution as a selectable agent for 6 days with leaves from transformed oilseed rape expressed the bar gene. Therefore, this study can contribute to the development of special oilseed rapes containing agriculturally useful traits such as herbicide resistance, drought tolerance, high yielding traits, and high oleic acid content.

• Journal of Plant Biotechnology
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• v.50
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• pp.56-62
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• 2023

Transgenic lilies have been obtained using Agrobacterium tumefaciens (AGL1) with the plant scale explants, followed by DL-phosphinothricin (PPT) selection. In this study, scales of lily plants cv. "red flame" were transformed with the pCAMBIA3301 vector containing the gus gene as a reporter and the blpR gene as a selectable marker, as well as a gene of interest showing herbicide tolerance, both driven by the CaMV 35S promoter. Using a 20-minute infection time and a 5-day cultivation period, factors that optimized and demonstrated a high transformation efficiency were achieved. With these conditions, approximately 22-27% efficiency was observed for Agrobacterium-mediated transformation in lilies. After transformation with Agrobacterium, scales of lilies were transferred to MS medium without selective agents for 2 weeks. They were then placed on selection MS medium containing 5 mg/L PPT for a month of further selection and then cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets after transferring into hormone-free MS medium. Also, most survived putatively transformed plantlets indicated the presence of the blpR gene by PCR analysis and showed a blue color indicating expression of the gus gene. In conclusion, when 100 scales of lily cv. "red flame" are transformed with Agrobacterium, approximately 22-27 transgenic plantlets can be produced following an optimized protocol. Therefore, this protocol can contribute to the lily breeding program in the future.

• The Journal of the Convergence on Culture Technology
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• v.10 no.3
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• pp.421-426
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• 2024

The Analytic Hierarchy Process (AHP) converts people's judgment criteria into objective numerical values using pairwise comparisons. However, the need for an excessive number of pairwise comparisons poses a problem. To mitigate this issue, most existing studies have utilized the process separation approach. The method of process separation devised in this study is a "separation and integration approach," where 1) the standard AHP process is used for evaluating judgment criteria, and 2) the Multi-Attributive Utility Technique (MAUT) is applied for comparing alternatives. This AHP-MAUT Hybrid model was applied to a real analysis case, specifically analyzing the transportation choices of commuters between Bundang and Gangnam Station in Gyeonggi Province. The results showed that the computational process was reduced by 42.03% when applying the Hybrid model compared to using the AHP model alone. Furthermore, the choice results of residents using the Hybrid model were compared with those using the standard AHP. The consistency between the two models' choices was 82.1%, indicating a significant level of consistency. In conclusion, this study contributes by presenting a simpler, more convenient, yet equally effective Hybrid model as a new decision-support system alternative to AHP.

• Journal of the Korean Society of School Health
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• v.37 no.1
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• pp.22-32
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• 2024

Purpose: The purpose of this study is to compare sociocultural attitudes toward appearance, appearance satisfaction, and peer relationships between male and female middle school students, and to compare the effects of these variables on self-esteem in students of different genders. Methods: Data were collected in August and September of 2023 and analyzed using SPSS 24.0. Data were collected from 289 male and 240 female students through a survey, and descriptive statistics, x2 test, t-test, Scheffé test, and multiple regression analysis were conducted. Results: Female students showed significantly higher scores for sociocultural attitudes toward appearance (3.06±0.77 vs. 2.82±0.74), significantly lower scores for appearance satisfaction (2.55±0.46 vs. 2.71±0.40), and significantly higher scores for peer relationships (3.46±0.69 vs. 3.00±0.78) than male students. Additionally, self-esteem was significantly lower in female students (2.84±0.59) than in male students (2.95±0.51). As a result of adjusting for weekly allowance, subjective academic achievement, family economic status, stress, and experience of sadness or despair, which were significant in the univariate analysis, self-esteem increased as appearance satisfaction (p for all <.001) and peer relationships increased in both male (p=.009) and female (p<.001) students. In addition, sociocultural attitudes toward appearance did not have a significant impact on self-esteem in both male and female students. Among general characteristics, weekly allowance, subjective academic achievement, and stress were found to have a significant effect on self-esteem in both genders. Relationships with parents had a significant effect on self-esteem only in male students, and experience of sadness or despair had a significant effect only in female students. Conclusion: In order to improve self-esteem in both boys and girls, appearance satisfaction and peer relationships should be improved. As boys with poor relationships with their parents and girls with high levels of sadness or despair are particularly likely to have low self-esteem, they are to be considered as a high-risk group.

• Journal of the Korean Institute of Landscape Architecture
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• v.39 no.6
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• pp.57-66
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• 2011

This study researched those universities for which fence opening and greening projects are being conducted by Seoul city. The forms of opened fences at 24 universities which have accomplished this project were classified into several types for each type of university, representative cases with many diverse facilities and active users were selected and investigated. The study was carried out using methods of field observations, literature review, and surveys. To maintain the confidentiality of the collected questionnaire analysis, the analysis of each type's usage frequency, overall satisfaction and a regression analysis with space environment and facilities, a one-way ANOVA for was used to validate the difference between types regarding satisfaction with the project. The results of usage type analysis were found to agree with the 3 analysis criteria-- installation location, user characteristics, and usage purpose--which were the legislative concepts. In overall satisfaction with facilities, it appeared that except for Seoul Women's College of Nursing with its rural district neighborhood type park, users were satisfied: with the small urban neighborhood park of Methodist Theological College, Konkuk University's small urban square park, and Sejong University's green space small city park. In general, users appeared to not have satisfaction with such features as fountains / hydroponic facilities, fitness facilities, and square facilities, which should be taken into consideration when pursuing further opening and greening projects. Regarding full satisfaction with the space environment, it was found that users were not satisfied with Seoul Women's College of Nursing's rural district neighborhood-style park, whereas they were satisfied with Methodist Theological College's small urban neighborhood park, Konkuk University's small urban square-style park, and Sejong University's green space small city park. In addition, it was shown that facilities use, convenience and privacy of the four parks were largely unsatisfactory for users, and that the small city parks located at roadsides were unsatisfactory regarding noise level, both of which should be most highly considered when conducting similar projects in the future.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.542-549
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• 2015

This work was carried out to evaluate the effects of preharvest 1-methylcyclopropene (1-MCP, $Harvista^{TM}$) and postharvest 1-MCP ($SmartFresh^{TM}$) treatments on the fruit quality attributes of cold-stored 'Fuji' ( Malus domestica Borkh.) apples. Fruits were exposed to 0, 95, 125, or $250 mg{\cdot}L^{-1}$ $Harvista^{TM}$ at 3, 2, 1 weeks before harvest (WBH), and treated with 0 or $1{\mu}{\cdot}L^{-1}$ $SmartFresh^{TM}$ at harvest. Fruit was then stored for up to 180 days at $0{\pm}1^{\circ}C$. Fruit fresh weight, Hunter's value a, internal ethylene concentration (IEC), flesh firmness, titratable acidity (TA), and soluble solids content (SSC) in fruit treated with $Harvista^{TM}$ were not different from those of control fruit at harvest. During cold storage, flesh firmness and TA were higher in fruit treated with $250mg{\cdot}L^{-1}$ $Harvista^{TM}$ at 2 and 3 WBH than in control fruit. IEC was 5.5-10.0% lower in fruit treated with $250mg{\cdot}L^{-1}$ $Harvista^{TM}$ at 2 and 3 WBH compared with control fruit as storage duration progressed, while SSC was not affected. Furthermore, flesh firmness, TA, and IEC were affected neither by $Harvista^{TM}$ nor $Harvista^{TM}+SmartFresh^{TM}$ treatments, compared with those fruit quality attributes at harvest. The correlation maps indicated that IEC was negatively correlated with firmness and TA, regardless of $Harvista^{TM}$ application levels. In addition, positive correlations between fruit quality attributes were detected in treatments with $250mg{\cdot}L^{-1}$ $Harvista^{TM}+SmartFresh^{TM}$. Therefore, the results suggest that with a single application of $SmartFresh^{TM}$, a higher level of $Harvista^{TM}$ application would help in retention of fruit quality attributes during cold storage.

• Journal of Korean Library and Information Science Society
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• v.5
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• pp.119-168
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• 1978

This is a survey of the reserved book system in the pilot universities in Korea. We have surveyed only 22 university libraries among 29 pilot schools as of 1977, because of the differences in the library users, library organization, library facilities, and library materials between universities and colleges. In 1972, the Korean Ministry of Education developed a reformation plan for their higher education based on the teaching method of curriculum-oriented faculty instead of that of the faculty-oriented curriculum. The former puts emphasis on the cultivation of a student's thinking, creativity, and judgement through self-teaching to do a given assignment. The reserved book system in a college or university library is one of the most important methods necessary to accomplish the above educational aim. The survey used a questionnaire with 50 question on 28 items concerning the various aspects of the reserved book system in 22 pilot universities. the survey result discovered many problems needing correction. The following list describes the measures needed to correct the problems found in the pilot universities. 1. The management of a centralized reserved book system is much more effective and economical than the decentralized reserved book system when a university is located on the same campus. 2. In the university library, an independent reserved book department requires to gain the desired educational aims as compared with the reserved book room controlled by any other department in the library. 3. The reserved book system should not be adopted by all the departments at once but enlarged gradually, for it needs the understanding and support of faculty members and the university itself. 4. As competence is essential to the effective operation of the reserved book room, the university library should not place an unqualified person in charge of the reserved book department. 5. The librarian in charge of the reserved book department is required to do more professional works such as analysis of users, collection and analysis of syllabuses, maintenance of faculty member cooperation, establishment of measures to acquire unavailable materials, and drawing up an effective management plan. However, he is spending most of his time in clerical works, that is, non-professional works. 6. Three to five titles of each reserved book are considered reasonable and required materials should be shelved in proportion to the number of students, that is, one copy per eight or ten students if the materials are allowed to lend for two hours at a time. For the supplementary materials, the library needs to place two or three copies per subject. 7. Professors must select reserved books with care so that they can be used year after year. 8. Few universities are asking professors the number of class students and the date when the reserved material will no longer be needed on reserve. 9. The library should gather all the lists of reserved books from every professor at least three to five months before the courses open, because it takes a long time to obtain foreign materials. 10. It is desirable that the reserved book department should collect the lists and prepare the materials with promptness and consistency. 11. Instead of block buying, it is desirable to purchase reserved books at the time the library gets the reserved book list from the professors. The library should also inform faculty members whether it obtained each reserved book or not before the course open. 12. The library should make a copy of materials if a professor requires to reserve an out-of-print book or partial contents of a book, journal, and thesis. 13. An independent budger for reserved books from the budget for general materials is desired. 14. The shelf arrangement of reserved books by courses or professors under the same department is much more preferable than a classified arrangement. 15. While most of the universities adopted the open shelves system for all the reserved books, it is more effective and economical to take a compromise system, that is, closed shelves for requires materials and open shelves for supplementary materials. 18. Circulation of reserved books needs a different system between required materials and supplementary materials: two or three hours and/or overnight loan for the former and two and/or three days loan for the latter. 17. A reserved book room should be open a long time after class so that students can have sufficient time to use the room. 18. The library must take daily and monthly statistic as well as statistics on every aspect of the reserved book system in order that the library ma decide on policy and management of the reserved book room in collaboration with the university. Furthermore, regular reports on the use of the reserved book room should be made to the president and the executive council by the library to acquire their understanding and cooperation for the reserved book system. 19. Cooperation of faculty members is indispensable to the effective management of the reserved book department and it is desirable to make a committee which will fix various decisions about the system. Whenever the director of the library make his decision, he must consult with his staff in order to involve them earnestly in the operation of the system.

• Journal of Plant Biotechnology
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• v.48 no.3
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• pp.179-185
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• 2021

Indoor gardening includes wall greening, terrariums, and flower arrangements. Among these types of indoor gardens, the terrarium is easy to access for the general public, but in Korea, because of the focus on esthetics, the original purpose of creating terrariums, which was to grow plants sustainably in an enclosed space, has been lost. In addition, miniaturization of plants is required to grow plants in an enclosed space. Since the available plant species suitable for a terrarium are limited, only plants such as succulents, cacti, and moss have been used. In this study, Bronze (X Graptosedum) was used, and these problems were solved using the following three methods: placement and growth of virus-free plants in the terrarium; extending the diversity of plants with minimal size that can be planted in terrariums; and reducing the price of in vitro plants with minimal size by achieving large-scale production. In particular, tissue-cultured succulents were developed into a Vivorium by replacing the tissue culture container and renewing the composition of the plant. This paper suggests a new indoor horticultural field, Vivorium, that can improve the current limitations of terrariums and make them more accessible to the general public. The introduction and popularization of new indoor gardening fields with the increase in single-person households and indoor activities in the Pandemic era can also improve psychological stability among people and in the society.

• Journal of Life Science
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• v.32 no.4
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• pp.271-278
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• 2022

The detailed mechanism by which cancer upregulated gene 2 (CUG2) overexpression induces cancer stem cell-like phenotypes is not fully understood. The downregulation of FBXW7 E3 ligase, a tumor suppressor known for its proteolytic regulation of oncogenic proteins such as cyclin E, c-Myc, Notch, and Yap1, has been frequently reported in several types of tumor tissues, including those in the large intestine, cervix, and stomach. Therefore, we investigated whether FBXW7 is involved in CUG2-induced oncogenesis. In this study, the decreased expression of FBXW7 was examined in human lung adenocarcinoma A549 (A549-CUG2) and human bronchial BEAS-2B cells (BEAS-CUG2) overexpressing CUG2 and compared with control cells stably expressing an empty vector (A549-Vec or BEAS-Vec). Treatment with MG132 (a proteosome inhibitor) prevented the degradation of FBXW7 and Yap1 proteins, which are substrates of the FBXW7 E3 ligase. To address the role of Fbxw7 in the development of cancer stem cell (CSC) phenotypes, we suppressed Fbxw7 protein levels using its siRNA. We observed that decreased levels of FBXW7 enhanced cell migration, invasion, and spheroid size and number in A549-Vec and BEAS-Vec cells. The enforced expression of FBXW7 produced the opposite results in A549-CUG2 and BEAS-CUG2 cells. Furthermore, the downregulation of FBXW7 elevated the activities of EGFR, Akt, and ERK1/2 and upregulated β-catenin, Yap1, and NEK2, while the enforced expression of FBXW7 generated the opposite results. We thus propose that FBXW7 downregulation induced by CUG2 confers CSC-like phenotypes through the upregulation of both the EGFR-ERK1/2 and β-catenin-Yap1-NEK2 signaling pathways.