• BMB Reports
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• 제45권8호
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• pp.470-475
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• 2012

Protein arginine methyltransferase 1 (PRMT1), a type-I arginine methyltransferase, has been implicated in diverse cellular events. We have focused on the role of PRMT1 in gliomagenesis. In this study, we showed that PRMT1 expression was up-regulated in glioma tissues and cell lines compared with normal brain tissues. The knock-down of PRMT1 resulted in an arrest in the G1-S phase of the cell cycle, proliferation inhibition and apoptosis induction in four glioma cell lines (T98G, U87MG, U251, and A172). Moreover, an in vivo study confirmed that the tumor growth in nude mouse xenografts was significantly decreased in the RNAi-PRMT1 group. Additionally, we found that the level of the asymmetric dimethylated modification of H4R3, a substrate of PRMT1, was higher in glioma cells than in normal brain tissues and decreased after PRMT1 knock-down. Our data suggest a potential role for PRMT1 as a novel biomarker of and therapeutic target in gliomas.

• Journal of Microbiology and Biotechnology
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• 제25권6호
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• pp.936-940
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• 2015

Human papillomavirus (HPV) type 52 is a high-risk HPV responsible for cervical cancer. HPV type 52 is common around the world and is the most common in some Asian regions. The available prophylactic HPV vaccines protect only from HPV types 16 and 18. Supplementing economical vaccines that target HPV type 52 may satisfactorily complement available prophylactic vaccines. A codon-adapted HPV 52 L1 gene was expressed in the methylotrophic yeast Hansenula polymorpha, which is used as an industrial platform for economical hepatitis B surface antigen particle production in China. We found that the recombinant proteins produced in this expression system could form virus-like particles (VLPs) with diameters of approximately 50 nm. This study suggests that the HPV 52 VLPs produced in this platform may satisfactorily complement available prophylactic vaccines in fighting against HPVs prevalent in Asia.

• Asian Pacific Journal of Cancer Prevention
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• 제13권4호
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• pp.1253-1259
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• 2012

The 14-3-3 proteins are highly conserved, ubiquitous molecules involved in a variety of biologic phenomena, such as cell cycle control, and apoptosis. However, their expression in cholangiocarcinoma has not been previously characterized. In this paper, immunohistochemistry using specific anti-14-3-3 monoclonal antibodies was performed on formalin-fixed;, paraffin embedded archival tissue from 86 patients of cholangiocarcinoma. We also examined the correlation between expression and survival rate and clinicopathologic factors such as tumor location, tumor size, pathologic differentiation, lymphatic permeation, lymph node metastasis, and tumor stage. Positive 14-3-3 proteins expression was observed for 6 isoforms (${\beta}$, ${\sigma}$, ${\gamma}$, ${\theta}$, ${\delta}$, ${\eta}$) of these proteins in 86 patients of cholangiocarcinoma. ${\beta}$ and ${\sigma}$ isoform immunoreactivity was correlated with lymph node metastasis, tumor stage and patients' survival rate. In addition, ${\delta}$ isoform immunoreactivity showed trends with tumor location, tumor size, pathologic differentiation and tumor stage, while the ${\theta}$ isoform was correlated with pathologic differentiation. These results indicated that upregulated expression of some isoforms of 14-3-3 may be a common mechanism for evading apoptosis in cholangiocarcinoma, so that targeting 14-3-3 may be a novel promising strategy for the treatment of this tumor.

• 노동경제논집
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• 제39권3호
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• pp.99-124
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• 2016

본 연구는 한국노동패널 자료를 사용하여 노동조합이 우리나라 여성근로자들의 임금에 미치는 영향을 실증분석하였다. 분석에는 여성근로자들의 임금함수 추정과정에서 경제활동참여에 의한 선택편의를 고려하였고, 종업원 수가 무응답인 경우 그 값을 보정하여 분석하였으며, 직장에 노동조합이 있고 노동조합에 가입한 조합원 근로자와 가입하지 않은 비조합원 근로자를 직장에 노동조합이 없는 근로자와 비교하여 분석하였다. 그 결과, 고정효과 모형 추정에서 조합원 및 비조합원 더미변수 모두 통계적으로 유의하지 않은 것으로 나타났다. 이로부터 OLS 모형 추정에서 나타난 노동조합의 임금효과는 능력이 높은 여성근로자들이 유노조 사업체에 취업하는 것을 더 선호한 결과이며, 비조합원의 무임승차 효과는 없다고 보는 것이 타당하다는 결론을 도출하였다.

• 한국콘텐츠학회논문지
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• 제10권4호
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• pp.386-396
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• 2010

본 연구의 목적은 최근 노사 간 이슈가 되고 있는 비정규직 이슈에 주목하여 비정규직 노사분규의 사례 중 대표적인 하이닉스 사내하청 노사분규사례의 발생원인 및 해결과정 등 전 과정을 살펴봄으로써 향후 유사한 노사분규에 대한 시사점을 주고자 함에 있다. 사례분석결과 하청노조는 교섭상대방인 하청회사가 존재 할 때까지는 하청회사들과 교섭하지만, 이후 하청회사는 폐업을 하게 되고 이에 따라 하청노조원들은 직장을 잃게 되고 교섭상대방은 원청회사로 바꾸어 직접교섭과 고용을 주장한다. 원청회사의 교섭거부와 하청노조의 불법을 포함한 전 방위적 공세 속에서 지역사회 등의 노력으로 중재가 시도되고 이후 사적 조정 그리고 최종 직접교섭으로 동 분규는 해결된다. 해결에 있어서는 고용을 배제되고 위로금 등으로 해결되어 불법과 무원칙이 난무하는 한국의 노사관계에서 나름대로 법과 원칙에 입각한 해결이라는 의미 있는 결과를 나타냈다. 마지막으로, 이상의 연구결과를 토대로 시사점을 제시하였다.

• 기록학연구
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• 제10호
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• pp.324-370
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• 2004

This paper aims to research the historical development of the archival systems in the Soviet Union from 1917 to the 1920's. The Russian revolution was the turning point not only for the political and social changes but for the archival administration in Russia, as it provided the new Soviet regime with the chances to reorganize the archival institutions under Bolshevik rule. However, we must not forget the fact that the Russian Archival Workers' Union had taken part in the organization of the archival systems together with the Bolshevik revolutionaries. The Soviet government intended to transform the decentralized and poorly organized archival systems in the prerevolutionary years into the centralized and bureaucratized ones. In this meaning, the decree signed be V. I. Lenin on 1 June 1918 was estimated as the real basis for the Soviet archival centralization. Lenin's archival decree of 1918 encompassed the entire national documentary legacy, which was gradually extended to all types and categories of manuscripts and archival records by successive legislation. The concept of state proprietorship of all documentary records was designated "Single State Archival Fond"(Edinyi Gosudarstvennyi arkhivnyi fond), which was renamed as "Archival Fond of the Russian Federation" after the collapse of the USSR. The independent state administrative agency, that is, "Main Administration of Archival Affairs"(Glavnoe upravlenie arkhivnym delom) was charged with the management of the entire "Single State Archival Fond". While the Soviet Union reorganized its archival systems in the twenties, the archival institutions became under the severe control of the government. For example, M. N. Pokrovskii, the well-known Marxist historian and the political leader, headed the organizational work of archives in the Soviet Union, which resulted in the exclusion of the prerevolutionary specialists from the archival field in the Soviet Union. However, the discussions over the concept of "the Archival Fond" by B. I. Anfilov helped to develop the archival theories in the USSR in the twenties. In conclusion, the Soviet Union, having emphasized the centralization of the archives, developed its archival systems from the early period, which were the basis of the systematic archival institutions in Russia.

• 디지털융복합연구
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• 제17권9호
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• pp.31-37
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• 2019

본 연구결과는 선행연구와 같이 피해자의 작업동기 상실, 조직 불신, 신경과민, 이직의도 증가, 좌절감, 스트레스와 감응저항, 무력감과 소외감을 나타냈다. 노동조합이라는 집단의 부정적인 힘에 눌려 일개 개인이 대항하거나 해결책을 찾을 수 없는 현실에 분노를 경험하였다. 또한 노조의 집단 따돌림에 대한 조합원의 두려움이라는 심리적 요인들이 파업에 합류하지 않은 노조원, 노조에 미가입한 근로자들에게 압력요소로 작용하여 기존 노동조합 노조원의 이탈을 방지하는데 영향을 미치는 것으로 나타났다. 노동조합의 집단따돌림 행동이 비록 집단의 목적을 달성하는데 효과가 있다고 하더라도 윤리적이어야 할 조직이 윤리적으로 벗어나는 모습을 보인다는 점에서 집단따돌림 행동이 타당한가에 대한 문제를 고려해 볼 필요가 있다.

• Journal of Ginseng Research
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• 제47권4호
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• pp.493-505
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• 2023

In recent years, an increasing number of reports have explored the wound healing mechanism of these two traditional Chinese herbal medicines- Panax ginseng and Panax notoginseng, but there is no systematic research on the related core functions and different mechanisms in the treatment of wound healing up to now. Based on network pharmacology and meta-analysis, the present work aimed to comprehensively review the commonality and diversity of P. ginseng and P. notoginseng in wound healing. In this study, a wound healing-related "ingredients-targets" network of two herbs was constructed. Thereafter, meta-analysis of the multiple target lists by Metascape showed that these two medicines significantly regulated blood vessel development, responses to cytokines and growth factors and oxygen levels, cell death, cell proliferation and differentiation, and cell adhesion. To better understand the discrepancy between these two herbs, it was found that common signaling pathways including Rap1, PI3K/AKT, MAPK, HIF-1 and Focal adhesion regulated the functions listed above. In parallel, the different pathways including renin-angiotensin system, RNA transport and circadian rhythm, autophagy, and the different metabolic pathways may also explained the discrepancies in the regulation of the above-mentioned functions, consistent with the Traditional Chinese Medicine theory about the effects of P. ginseng and P. notoginseng.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 추계학술대회
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• pp.445-446
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• 2021

유니온 파일 시스템은 다양한 파일과 디렉토리를 통합하여 단일 파일 시스템으로 사용할 수 있는 기술로, 통합에 사용되는 Source File/Directory를 그대로 유지할 수 있는 장점이 있어 컨테이너 플랫폼 등의 많은 어플리케이션에서 사용되고 있다. 유니온 파일 시스템을 사용할 때, 사용자는 Write-able Layer에 접근하며, 여기에는 운영체제에서 제공하는 보안 기술을 적용할 수 있다. 그러나 유니온 파일 시스템 생성에 사용되는 Source File과 Directory에는 별도의 보안 기술을 적용하기 어려운 단점이 있다. 본 연구에서는 유니온 파일 시스템 사용 시 발생될 수 있는 Source File/Directory에 대한 보안 위협을 차단하기 위한 접근통제 메커니즘을 제안하고자 한다. 접근통제 메커니즘의 실효성을 검증하기 위해 실증 구현한 후 기능 테스트를 수행한 결과, 본 연구에서 제안한 접근통제 메커니즘은 유니온 파일 시스템의 장점을 유지하면서 Source File/Directory를 보호할 수 있는 것으로 확인되었다.

• Korean Journal of Radiology
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• 제21권4호
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• pp.483-493
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• 2020

Objective: To evaluate the distribution and characteristics of peripheral nerve abnormalities in chronic inflammatory demyelinating polyneuropathy (CIDP) using magnetic resonance neurography (MRN) and to examine the diagnostic efficiency. Materials and Methods: Thirty-one CIDP patients and 21 controls underwent MR scans. Three-dimensional sampling perfections with application-optimized contrasts using different flip-angle evolutions and T1-/T2- weighted turbo spin-echo sequences were performed for neurography of the brachial and lumbosacral (LS) plexus and cauda equina, respectively. Clinical data and scores of the inflammatory Rasch-built overall disability scale (I-RODS) in CIDP were obtained. Results: The bilateral extracranial vagus (n = 11), trigeminal (n = 12), and intercostal nerves (n = 10) were hypertrophic. Plexus hypertrophies were observed in the brachial plexus of 19 patients (61.3%) and in the LS plexus of 25 patients (80.6%). Patterns of hypertrophy included uniform hypertrophy (17 [54.8%] brachial plexuses and 21 [67.7%] LS plexuses), and multifocal fusiform hypertrophy (2 [6.5%] brachial plexuses and 4 [12.9%] LS plexuses) was present. Enlarged and/or contrast-enhanced cauda equina was found in 3 (9.7%) and 13 (41.9%) patients, respectively. Diameters of the brachial and LS nerve roots were significantly larger in CIDP than in controls (p < 0.001). The largest AUC was obtained for the L5 nerve. There were no significant differences in the course duration, I-RODS score, or diameter between patients with and without hypertrophy. Conclusion: MRN is useful for the assessment of distribution and characteristics of the peripheral nerves in CIDP. Compared to other regions, LS plexus neurography is more sensitive for CIDP.