• The Journal of the Acoustical Society of Korea
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• v.36 no.3
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• pp.172-178
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• 2017

Ultrasound has been widely used for biological and medical applications including induction of cell death, but a precise mechanism of induced cell death by ultrasound is controversial. In this study, an irradiation system with 40 kHz ultrasound was developed for a suitable cell death test of a representative unicellular organism, yeast, and used to study the biological effect of ultrasound on inducing cell death. Potassium Iodide (KI) dosimetry was used to devise an optimal system that successfully delivers 40 kHz ultrasound and produces reactive oxygen species in a 1.5 ml Eppendorf tube. Cell death was observed in an ultrasound transmission time-dependent fashion in this system. Thermal effect during irradiation was not observable in ultrasound induced cell death. Co-treatment of 40 kHz ultrasound and hydrogen peroxide showed a synergistic effect in inducing cell death. This finding suggests that 40 kHz ultrasound is related to reactive oxygen species formation. However, NAC (N-acetyl-L-cysteine) oxygen scavenger slightly inhibited the cell death by 40 kHz ultrasound. It was also found that 40 kHz ultrasound induced cell death was slightly inhibited by inhibitors of necrosis or apoptosis (glycyrrhizin or zVAD-fmk). This study suggests that cell death induced by 40 kHz ultrasound may not be exclusively related to reactive oxygen species formation and thermal effects in irradiated yeast cells.

• Journal of Biomedical Engineering Research
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• v.33 no.1
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• pp.32-38
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• 2012

Recently, several ultrasound imaging techniques for tissue characterization have been developed. Among them, ultrasound elastography is regarded as the most promising modality and has been rapidly developed. One of ultrasound elastography techniques is shear modulus imaging. Normal and cancerous tissues show big difference of shear moduli and they have good image contrast. However shear wave elastography requires more complicated hardware and more computations for image reconstruction algorithm. Therefore new efficient techniques are being developed. In this paper, we have developed a very flexible ultrasound system for elastography experiments. The developed system has capabilities to acquire ultrasound RF data of all channels and generate arbitrary ultrasound pulse sequences. It has a huge amount of memories for RF data acquisition and a simple and flexible pulse generator. We have verified the performance of the system showing conventional B-mode images and preliminary results of elastography. The developed system will be used to verify our own reconstruction algorithm and to develop more efficient elastography techniques.

• Journal of the Korean Society of Physical Medicine
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• v.6 no.3
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• pp.303-310
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• 2011

Purpose : This study examined the feedback effect of real-time ultrasound imaging on the thickness of transversus abdominis(TrA), internal abdominal oblique(IO) and external abdominal oblique(EO) during abdominal hollowing exercise(AHE) in crook lying. Methods : We performed this study on 30 healthy men who voluntarily consented to participate in this study after listening to its purpose and method. All subject were divided into an experimental group(n=15) with using the real-time ultrasound imaging feedback(RUIF) and a control group(n=15) without the RUIF The thickness changes between rest and AHE were compared between the two groups in crook lying. Results : The difference in TrA and EO thickness changes between the groups were significant in crook lying (p<0.05). Conclusion : The group with using real-time ultrasound imaging feedback showed a higher increase in the thickness of TrA than the other group without real-time ultrasound imaging feedback. And the thickness of EO in the group with using real-time ultrasound imaging feedback decreased than the other group without real-time ultrasound imaging feedback. If the muscle thickness can be regarded as an indicator of muscle activity, RUIF will be helpful for inducing the independent activity of TrA by reducing the activities of abdominal muscles such as EO.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.2
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• pp.493-497
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• 2012

This study was conducted to retrospectively evaluate the pattern of contrast enhancement with SonoVue on gray-scale ultrasonography of hepatic angiomyolipoma (HAML). Imaging features of 33 pathologically proven HAML lesions in 33 patients who underwent baseline ultrasound and contrast-enhanced ultrasonography (CEUS) were assessed retrospectively. All lesions were enhanced in the arterial phase and showed whole-tumor filling in. Thirty-two of 33 (97%) lesions showed early positive enhancement in the arterial phase. Twenty-three of these exhibited isoechoic or hyperechoic features in the portal phase. HAML demonstrate characteristic manifestations with SonoVue-enhanced real-time gray-scale ultrasonography.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.27 no.2
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• pp.103-110
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• 2001

This study was performed to observe the effect of ultrasound(1.0MHz, $0.75W/cm^2\;and\;1.0W/cm^2$) irradiation on cultured MC3T3-E1 cell, osteoblastic like cell with respect to the proliferation, protein synthesis, and alkaline phosphatase activity of the cells. The results were as follows: 1. The proliferation of MC3T3-E1 cells was increased on ultrasound irradiated group compared with control group. 2. The protein synthesis was not apparently increased on ultrasound irradiated group compared with control group. 3. The alkaline phosphatase activity level was not apparently increased on ultrasound irradiated group compared with control group. From the above results and other literatures, we could suggest that the ultrasound with the appropriate intensity and frequency may have important roles in stimulation of cell proliferation. Therefore the ultrasound may be used in the acceleration of the bone regeneration and bone fracture healing.

• The Journal of Korean Physical Therapy
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• v.14 no.1
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• pp.55-62
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• 2002

The purpose of this study was to determine the effects of the ultrasound on treatment of chronic sinusitis. 24 persons of men and women with chronic sinusitis divided into three groups of each 8 persons randomly and when were they were treated with the medication, the ultrasound(1MHz continuous wave, 1W/$cm^2$ intensity, three times per a week and once of three minutes circular stroke method) and the medication+ultrasound for 2, 4, 6 and 8 weeks. The results of this study were ad follows. 1. There was not statistical significant differences between the effects of treatment until two weeks(P>.05). 2. There was statistical difference between the effects of treatment after 4 weeks, 6 weeks and 8 weeks(P<.05). There was statistical significant difference between the group with ultrasound treatment and the group with ultrasound+medication treatment and there was statistical significant difference between the group with medication treatment and the group with ultrasound+medication treatment but there was not statistical significant between the group with ultrasound treatment and the group with medication treatment. 3. After 4 weeks, the highest effect for chronic sinusitis was group

• Journal of Biomedical Engineering Research
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• v.36 no.5
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• pp.162-168
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• 2015

In this study, we suggested the performance and safety testing guideline for low intensity pulsed ultrasound (LIPUS) represented by the ultrasound fracture treatment device and cartilage treatment device and low intensity focused ultrasound (LIFU) represented by ultrasonic face lifting device. For these study, the international standards and management regulations of Korea, Japan and United State were analyzed. And the usefulness and applicability were evaluated by testing with commercial equipment and reflecting the views of the industry and experts. As a result of this study, the safety and performance test guidelines for low intensity therapeutic ultrasound device were proposed by presenting the 10 items for LIPUS and 12 items for LIFU. The suggested guidelines are considered a high utilization in the domestic testing and approval authorities. And they are also thought to be useful to new technology development.

• The Journal of Korean Orthopaedic Ultrasound Society
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• v.6 no.1
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• pp.38-44
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• 2013

The ultrasound can be used primarily to guide exact needle placement for aspirations of fluids, injections of steroid, and biopsies in musculoskeletal field. Recently, ultrasound-guided intervention is widely used because of several advantages such as real-time performance, relatively inexpensiveness, and getting multiple images without additional radiations. However, the modality is operator dependent and requires detailed knowledge of the relevant anatomy and there have been also reported serious complications related to the procedure. So, authors will discuss about the basic techniques, principles and cautions for the use of ultrasound-guided intervention in musculoskeletal field.

• Journal of Korean Physical Therapy Science
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• v.10 no.1
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• pp.170-179
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• 2003

This study was performed that how phonophoresis using ultrasound for piroxicam affects transdermal permeation and anti-inflammative effects. Transdermal permeation study conducted by using hairless mouse had two categories: control group and ultrasound group. Transdermal permeation was observed according to duty cycle and intensity. Anti-inflammatory effects were determined using in Sprague-Dawley rat. The subjects were divided into three groups of six SD rat each 24 hour, 48 hour, 72 hour. The results of this study were as follows: 1. Transdermal permeation of piroxicam was measured according to ultrasound duty cycle. This research demonstrates that ultrasound group retains more transdermal permeation than control group, and that pulsed ultrasound group holds a little more transdermal permeation than continuous ultrasound group. 2. The transdermal permeation of piroxicam is closely related with ultrasound intensity. Effect of each group of transdermal permeation was significant rises in proportion to ultrasound intensity. 3. By observing inflammation of the tissue caused by trauma, phonophoresis group showed more significant of anti-inflammatory effect. The conclusion of phonophoresis was found to improve significantly the transdermal permeation and the anti-inflammatory effect.

• The Journal of Korean Orthopaedic Ultrasound Society
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• v.6 no.2
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• pp.81-93
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• 2013

This review was described to investigate the feasibility of using ultrasound as an image tool for interventions of lumbar spine. This article will first provide an overview of lumbosacral spine surface anatomy and sonoanatomy. A detailed understanding of anatomy is critical for interpretation of ultrasound and procedural performance at spine. Fluoroscopy is most commonly used in interventional spine procedures, but radiation exposure is the major concern when obtaining fluoroscopic images. Ultrasound is radiation-free, is easy to use, and can provide real-time images with high accuracy. Also this device can be used in virtually any clinical setting. Ultrasound guidance offers a reliable alternative to fluoroscopy or computed tomography for lumbar interventions and can be safely performed without radiation exposure.