• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1125-1129
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• 2005

Cloning of a 6.6-kb BamHI digested chromosomal DNA from S. griseus IFO13350 revealed the presence of an additional gene encoding a novel trypsin-like enzyme, named SprU. The SprU protein shows a high homology ($79\%$ identity, $88\%$ similarity) with the SGT protease, which has been reported as a bacterial trypsin in the same strain. The amino acid sequence deduced from the nucleotide sequence of the sprU gene suggests that SprU is produced as a precursor consisting of an amino-terminal presequence (29 amino acid residues), prosequence (4 residues), and mature trypsin consisting of 222 amino acids with a molecular weight of 22.94 kDa and a calculated pI of 4.13. The serine, histidine, and aspartic acid residues composing the catalytic triad of typical serine proteases are also well conserved. When the trypsin activity of the SprU was spectrophotometrically measured by the enzymatic hydrolysis of the artificial chromogenic substrate, N-${alpha}$-benzoyl-DL-arginine-p-nitroanilide, the S. lividans transformant with pWHM3-U gave 3 times higher activity than that of control. When the same recombinant plasmid was introduced into S. griseus, however, the gene dosage effect was not so significant, as in the cases of other genes encoding serine proteases, such as sprA, sprB, and sprD. Although two trypsins, SprU and SGT, have a high degree of homology, the pI values, the gene dosage effect in S. griseus, and the gene arrangement adjacent to the two genes are very different, suggesting that the biochemical and biological function of the SprU might be quite different from that of the SGT.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.76-76
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• 1995

Virus-encoded HIV-1 reverse transcriptase (RTase) is one of the major targets for the development of drugs for HIV-1 since it is an essential enzyme-for the replication cycle of HIV-1. We cloned the entire reverse trancriptase gene into an inducible expression vector with tac promotor= RTase was stably overexpressed and induced by IPTG and the highly-expressed RTase was purified partially by use of DEAE cellulose and Mono Q column. The partially purified enzyme (663kDa, 51kDa) as exhibited by SDS-PAGE showed the high specific activity (16,570U/mg) when the assay for the RTase activity was carried out using $^3$H-dTTP and poly(rA): oligo(dT)12-18 as the substrate.

• Journal of Korean Theatre Studies Association
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• no.48
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• pp.171-196
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• 2012

In der vorliegende Arbeit wird die neue politische $M{\ddot{o}}glichkeit$ des Theaters untersucht. Wie die politische Propaganda, die Volk mit den absoluten Dogmen zu ${\ddot{u}}berzeugen$, immer seltner geworden ist, haben die alten Versuche des Theaters, ein Publikum mit einem Botschaft $aufzukl{\ddot{a}}ren$ und zu ${\ddot{u}}berreden$, ihre ehemalige ${\ddot{U}}berzeugungskraft$ verloren. In der heutigen Welt kann Theater leider nicht als eine moralische Anstalt mehr funktioniert. An diesem Endpunkt, wo Theater heute anzukommen scheint, gibt es aber auch Versuche, Theater auf neue Weise politisch zu machen. Es handelt sich hier $nat{\ddot{u}}rlich$ nicht um Wiedergabe einer ideologischen Diskurs mehr, sondern um die $St{\ddot{o}}rung$ der Wahrnehmung der Zuschauer durch das $Enth{\ddot{u}}llung$ des Anderen, den die System des Neo-liberalismus $verh{\ddot{u}}llt$. Damit der Anderen aufgezeigt wird, machen die verschiedene theatralische Experimente die $B{\ddot{u}}hne$ zu einem leeren Raum, indem sie den $gew{\ddot{o}}hnlichen$ dramatischen Rahmen zu $zerst{\ddot{o}}ren$ versucht. In diesem Raum wird die Vorstellungskraft der Zuschauer erst aktiviert, dadurch die Zuschauer selbst entscheiden $k{\ddot{o}}nnenn$, was sie verstehen $m{\ddot{o}}chten$ und wie sie sich dazu verhalten sollen. All diese Prozesse $m{\ddot{u}}ssen$ die Zuschauer auch als Individuum konstruieren. Die von tradtionellen Theater $getr{\ddot{a}}umte$ Zuschauergemeinschaft, die der Botschaft aus der $B{\ddot{u}}hne$ $gleichm{\ddot{a}}{\ss}ig$ zugestimmt hat, ist daher nicht mehr da. Im heutigen Theater handelt es sich um jeden Zuschauer, der selbst aktiv handelt. Solche Subjekt ist die echte demokratische, die in der heutigen politischen Wissenschaft mehrmals betont wird.

• Structural Engineering and Mechanics
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• v.85 no.5
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• pp.679-691
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• 2023

Many codes allow designers to use the bending moment diagram computed by elastic analysis and modify it by a certain amount of moment redistribution (MR) to account for plastic behaviour of continuous beams. However, several researchers indicated that the MR at the ultimate limit state (𝛽_u) for some beams deviate significantly from the specified values of various codes. This paper examines the applicability of the provisions on 𝛽_u in ACI 318-19 and Eurocode 2 through numerical investigations and comprehensively explores the influencing factors. The results show that some parameters not considered in those codes influence 𝛽_u to a certain extent, where the ratio of tensile reinforcement ratio at intermediate support to tensile reinforcement ratio at midspan (𝜌_s1/𝜌_s2) and load type are crucial parameters to consider. The specific combination of these two parameters may make the codes overestimate or significantly underestimate the 𝛽_u. On the other hand, the yield state of both critical sections is found to have an important influence on the influence degree of each parameter on 𝛽_u. The yield conditions are investigated, and an empirical judgment equation is proposed. In addition, the influence laws of the critical parameters on 𝛽_u have been further proved by theoretical derivation. Finally, due to 𝜀_t is found to have a better linear correlation with 𝛽_u than x_u/d, equations as a function of 𝜀_t for predicting the 𝛽_u of continuous beams under the two loads are proposed, respectively.

• Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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• v.2
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• pp.285-306
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• 2000

In der vorliegenden Arbeit wurde die Auffassung motiviert, dass es sich bei der deutschen $K\"{o}rpersprache$ in semiotischer Hinsicht um kommunikative Geste handelt. Die semio-syntaktischen, -semantischen und -pragmatischen Charakteristika dieser Geste wurden erortert. Insbesondere die ermittelten syntaktischen und semantischen Eigenschaften der $K\"{o}rpersprache\;sprechen\;f\"{u}r$ die Auffassung, dass sie in semio-syntaktischer, -semantischer und -pragmatischer Hinsicht mit Interjektionen $verkn\"{u}pfen\;k\"{o}nnen$. Beispielsweise wurde eine deutsche Geste 'Telefone handhaben' $angef\"{u}hrt$. Bei dieser Geste tritt eine deutsche vokalische Interjektion 'Klingeling' auf. Diese Geste stellt drei Ausdrucksvariationen(1- 3) und vier Bedeutungsvaritionen(A-D) dar: 1) Die Faust neben das Ohr halten, 2) Daumen neben das Ohr und Kleinfinger neb en den Mund halten und 3) Mit dem Zeigefinger var dem $K\"{o}rper$ frontal kreisen; A) Telefonieren thematisieren, B) Einen Telefonanruf aufforden, C) Zu einen Telefonanruf aufforden und D) Den Eingang eines Anrufs an zeigen. Mit Hilfe der semiotischen Gestenanalyse $k\"{o}nnen$ die deutschen $K\"{o}rpersprache$ mit Interjektionen alphabetisch geordnet werden, und die Gestenliste soll $f\"{u}r$ den DaF-Unterricht von Nutzen sein.

• Journal of Microbiology and Biotechnology
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• v.28 no.6
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• pp.1030-1036
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• 2018

Bacillus strains produce various types of antibiotics, and random mutagenesis has traditionally been used to overproduce these natural metabolites. However, this method leads to the accumulation of unwanted mutations in the genome. Here, we rationally designed a single nucleotide substitution in the degU gene to generate a B. subtilis strain displaying increased plipastatin production in a foreign DNA-free manner. The mutant strain (BS1028u) showed improved antifungal activity against Pythium ultimum. Notably, pps operon deletion in BS1028u resulted in complete loss of antifungal activity, suggesting that the antifungal activity strongly depends on the expression of the pps operon. Quantitative real-time PCR and lacZ assays showed that the point mutation resulted in 2-fold increased pps operon expression, which caused the increase in antifungal activity. Likewise, commercial Bacillus strains can be improved to display higher antifungal activity by rationally designed simple modifications of their genome, rendering them more efficient biocontrol agents.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.3
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• pp.634-639
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• 2007

Despite continuous changes these days, exhibit management methods have not changed that much. To solve th problem, some systems like bar code, color code, IrDA, etc. are being used, but still many problems remain. For the problem-solving, "Exhibt Management System with RFID" is designed and its prototype is developed, which can reduce resources and efforts to manage exhibits through that an operator confirms exhibit information and inputs any damage on the exhibit on the site by using RFID system. The system purposes to improve efficiency of exhibit management by allowing an operator to check all information of exhibitions in real time using a portable reader that recognizes tags of 900MHz band manually attached to each exhibit.

• Journal of The Korean Association of Information Education
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• v.13 no.1
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• pp.41-49
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• 2009

It's going to be ubiquitous environment which is able to use web pages independent of time and a place by recent development of mobile techniques. On this, we improved the leveled reading supporting system according to U-learning environment and make student to read on both Off-line and On-line through connecting to E-book service. So we developed the reading supporting system which can improve the interests in reading and reading skill and proved the effects. U-learning based leveled reading education support system could be helped develop the reading ability by raising the interest in the activities reading and after reading.

• Korean Journal of Food Science and Technology
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• v.36 no.6
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• pp.1008-1013
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• 2004

Effects of extraction conditions and molecular fractionation on anticoagulant activities of major brown seaweeds in Korea were investigated. Hot water extracts of C. costata, U. pinnatifida (Sporophyte), L. japonica, K. crassifolia, E. stolonifera, E. bicyclis, S. horneri, and E. kurome increaced activated partial thromboplastin time (APTT) over 190 seconds, which may be related to intrinsic pathway of blood coagulation. Hot water extract of E. Kurome (EKJ) was further fractionated by ethanol precipitation. EKJ-eim, ethanol-insoluble material of EKJ, showed higher anticoagulant activity than EKJ. EKJ-eim was further fractioned with ultrafiltration. EKJ-eim 1, (over 100 kDa) fraction showed higher APTT activity than EKJ-eim. A EKJ-eim 1 was sulfated polysaccharide consisting of fucose, xylose, mannose, galactose, glucose and, sulfate at molar ratio of 1 : 0.05 : 0.10 : 0.15 : 0.17 : 1.46. The anticoagulant activity increased as sulfate content and molecular weight increased.

• Journal of Adhesion and Interface
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• v.1 no.1
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• pp.38-46
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• 2000

A novel diamine monomer, containing fluorine and phosphine oxide, bis(3-aminophenyl) 3,5-bis(trifluoromethyl) phenyl phosphine oxide (mDA6FPPO), was prepared via Grignard reaction, and utilized to prepare polyimides with dianhydrides such as PMDA, 6FDA, BTDA or ODPA, by the conventional two-step route; preparation of poly(amic acid), followed by solution imidization. The polyimides were characterized by FT-lR, NMR, DSC and DMA, with intrinsic viscosity, and adhesive properties were also evaluated. A phosphine oxide containing monomer, bis(3-aminophenyl) phenyl phosphine oxide (mDAPPO) a nd a commercial 3,3'-diamino diphenyl sulfone (mDDS) were also used for comparison. The polyimides with mDA6FPPO exhibited high $T_g$, excellent solubility, and good adhesive properties.