• 약학회지
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• 제45권6호
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• pp.724-729
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• 2001

Melanogenesis is a physiological process resulted in the synthesis of melanin pigments, which has a role in protecting skin front the damaging effect of ultra-violet (UV) radiation. The main aim of the present study was to examine the effect of Ulmus davidiana var. japonica(UL) on Melanogenesis. Cells were cultured in the presence of various concentrations of Ulmus davidiana var. japonica for 48 h, and there were estimated total melanin contents as a final product and activity of tyrosinase, a key enzyme, in Melanogenesis. Among the four solvent extracts tested, EtOAc extract mostly increased tyrosinase activity, And EtOAc extract increased the melanin contents and tyrosinase activity in a dose-dependent manner. Especially It was observed that 100$\mu\textrm{g}$/ml EtOAc extract promotes melanin secretion in B16/F10 melanoma cells by 140% at 48 h treatment and activity of tyrosinase increased by 180% in the presence of same concentration. In conclusion, as for EtOAc extract treatment, there was no effect on the viability of B16/F10 cell, only to stimulate Melanogenesis.

• 한방안이비인후피부과학회지
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• 제14권1호
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• pp.209-225
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• 2001

Recently many efforts were focused to understand the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin biosynthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) or cAMP-elevating agents stimulate melanogenesis and enhance the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Radix Trichosanthis on the basal melanogenic activities of B16/F10 mouse melanoma cells, and on the ${\alpha}$-MSH or forskolin-induced melanogenesis. Radix Trichosanthis alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. Pretreatment of the cells with Radix Trichosanthis also suppressed the increase of ${\alpha}$-MSH (10 nM) or forskolin (20${\mu}M$)-induced melanin content and tyrosinase activity. The decrease in the tyrosinase activity was paralled by a decrease in the abundance of tyrosinase protein and tyrosinase promoter activity. Pretreatment of the cells with Radix Trichosanthis also inhibited the increase of forskolin($20{\mu}M$) induced the amount of tyrosinase protein and tyrosinase promoter activity. The results of DOPA staining revealed that pretreatment of the cells with Radix Trichosanthis showed less intensity than B16 melanoma cells stimulated with ${\alpha}$- MSH or forskolin. These results suggest that Radix Trichosanthis inhibits melanogenesis and abrogates ${\alpha}-MSH and cAMP-induced melanogenesis in B16 melanoma cells.

• 한방안이비인후피부과학회지
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• 제17권1호
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• pp.82-93
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• 2004

Recently many efforts were focused to understanding the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin biosynthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) or cAMP-elevating agents stimulate melanogenesis and enhance the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Radix Trichosanthis on the basal Melanogenic activities of Bl6/F10 mouse melanoma cells, and on the ${\alpha}$-MSH or forskolin-induced melanogenesis. Radix Trichosanthis alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. Pretreatment of the cells with Radix Trichosanthis also suppressed the increase of ${\alpha}$-MSH(10 nM) or forskolin(20 ${\mu}$M)-induced melanin content and tyrosinase activity. The decrease in the tyrosinase activity was paralled by a decrease in the abundance of tyrosinase protein and tyrosinase promoter activity. Pretreatment of the cells with Radix Trichosanthis also inhibited the increase of forskolin(20 ${\mu}$M) induced the amount of tyrosinase protein and tyrosinase promoter activity. The results of DOPA staining revealed that pretreatment of the cells with Radix Trichosanthis showed less intensity than B16 melanoma cells stimulated with ${\alpha}$-MSH or forskolin. These results suggest that Radix Trichosanthis inhibits melanogenesis and abrogates ${\alpha}$-MSH and cAMP-induced melanogenesis in B16 melanoma cells.

• 한방안이비인후피부과학회지
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• 제16권3호
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• pp.129-144
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• 2003

Recently many efforts were focused to understand the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin biosynthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) or cAMP-elevating agents stimulate melanogenesis and enhance the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Rhizoma Bletillae on the basal melanogenic activities of B16／F10 mouse melanoma cells, and on the ${\alpha}$-MSH or forskolin-induced melanogenesis. Rhizoma Bletillae alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. Pretreatment of the cells with Rhizoma Bletillae also suppressed the increase of ${\alpha}$-MSH (100 nM) or forskolin (20 ${\mu}M$)-induced melanin content and tyrosinase activity. The decrease in the tyrosinase activity was paralled by a decrease in the abundance of tyrosinase protein and tyrosinase promoter activity. Pretreatment of the cells with Rhizoma Bletillae also inhibited the increase of forskolin(20${\mu}M$) induced the amount of tyrosinase protein and tyrosinase promoter activity. The results of DOPA staining revealed that pretreatment of the cells with Rhizoma Bletillae showed less intensity than B16 melanoma cells stimulated with ${\alpha}$-MSH or forskolin. These results suggest that Rhizoma Bletillae inhibits melanogenesis and abrogates ${\alpha}$-MSH and cAMP-induced melanogenesis in B16 melanoma cells.

• 한국자원식물학회지
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• 제34권4호
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• pp.368-376
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• 2021

흰민들레 추출물에서 분리한 taraxinic acid, dihydrotaraxinic acid 및 luteolin를 대상으로 화장품의 미백 소재로서의 이용 가능성을 조사하였다. In vitro tyrosinase 저해 활성은 100 ㎍/mL에서 대조군인 ascorbic acid는 42% 저해 활성을 보였으며, luteolin은 48%, taraxinic acid는 29%, 그리고 dihydrotaraxinic acid는 18%를 보여 luteolin이 가장 높게 나타냈다. Taraxinic acid와 dihydrotaraxinic acid는 100 ㎍/mL 이하의 조건에서 B16BL6 멜라닌 형성 세포의 생존율에 영향을 미치지 않았으나, luteolin은 강한 세포 독성을 나타냈다. Taraxinic acid와 dihydrotaraxinic acid가 세포 생존율에 영향을 미치지 않는 농도 범위(10, 50, 100 ㎍/mL)에서 B16BL6 세포 내 멜라닌 생합성 및 tyrosinase 활성을 확인한 결과 농도 의존적으로 억제하는 것으로 확인되었다. 특히 taraxinic acid는 tyrosinase inhibitor로 알려진 arbutin보다 뛰어난 저해 활성을 나타내어, 천연 기능성 미백 화장품 원료로 사용 가능성이 기대된다.

• 환경위생공학
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• 제13권1호
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• pp.157-165
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• 1998

The purpose of this study is to explain the relations between pine needle tyrosinase's activity and quantity of minerals in the Waters' mineral water. Pine needle tyrosinase's activity was measured by metal ions' concentration like Ca, Mg, Na, K, and Fe in different kinds of drinking water. 1. Pine needle tyrosinase has the highest activity when Ca's concentration is 14.40mg/L while the activity decreases by 92% when it is 108.10 mg/L. Therefore, the resonable range of Ca concentration for drinking water is 10-100.0 mg/L. 2. Mg has higher Pine needle tyrosinase's activity than Ca by three times. The reasonable range of Mg concentration for drinking water is 3.0-10.0 mg/L. 3. Pine needle tyrosinase has the highest activity when Na's concentration is 15.70 mg/L. The reasonable range of Na concentration for drinking water is less than 15 mg/L. 4. The activity increases as K concentration rises. In normal kinds of drinking water, K concentration is less than 10 mg/L. Since K has impacts on the activity only when its concentration is more than 10 mg/L, no problem in expected. 5. Fe has some impacts on the activity when its concentration is more than 10mg/L. As most kinds of drinking water contain less than 0.3 mg/L, no problem is expected. With above-mentioned observations, it is concluded that Water's mineral water contains reasonable levels of minerals like Ca, Mg, K and Na.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.151.2-151.2
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• 2003

For the purpose of the development of a skin-whitening agent, Sophora flavescens was evaluated for tyrosinase inhibitory activity and its active principles were identified followed activity-guided isolation. The ethanol extract and dichloromethane fraction from S. flavescens showed significant inhibition of mushroom tyrosinase. From the dichloromethane fraction, three known prenylated flavonoids, sophoraflavanone G, kuraridin, and kurarinone, were isolated. Compared with kojic acid ($IC_50$=20.5 $\mu$M), these compounds possessed more potent tyrosinase inhibitory activity. (omitted)

• 생약학회지
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• 제30권4호
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• pp.397-403
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• 1999

Tyrosinase plays an important role in the process of melanin polymer biosynthesis. Therefore, the enzyme inhibitors have been of great concern as cosmetics to have skin-whitening effects on the local hyperpigmentation. During the search for new inhibitory compounds on melanin polymer biosynthesis from natural sources, MeOH extracts of 589 higher plants were tested for the inhibitory effect on tyrosinase activity by the muschroom tyrosinase assay in vitro. Among plants tested, the leaves of Cercis chinensis exhibited potent inhibitory effect on mushroom tyrosinase activity. Subsequently seven active compounds were isolated from the ethyl acetate soluble part of acetone extract of the leaves of C. chinensis by the activity guided fractionation monitoring the inhibitory effect on tyrosinase activity. Their chemical structures were identified as $kaempferol-3-0-{\alpha}-L-rhamnoside$, quercitrin, $myricetin-3-0-{\alpha}-L-rhamnoside$, myricetin-3-0-(2'-O-galloyl)- ${\alpha}$ -L-rhamopyranoside (desmanthin), (-)-epicatechin-3-0-gallate, (-)-epigallocatechin-3-0-gallate, and methyl gallate on the basis of the speculation of spectral data and chemical reaction. Among the flavonol rhamnosides, myricetin-3-0-(2'-O-galloyl)- -L-rhamnoside(desmanthin) showed most potent inhibitory effect on tyrosinase activity and the structure of B-ring in flavonol moiety was related to the activity. (-)-Epigallocatechin-3-O-gallate having pyrogallol group in flavan-3-ol moiety exhibited more potent inhibitory effect than (-)-epicatechin-3-0-gallate having catechol group in flavan-3-ol moiety on mushroom tyrosinase activity.

• 한국식품영양과학회지
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• 제38권3호
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• pp.292-296
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• 2009

Tyrosinase는 피부의 melanocytes에서 멜라닌 생성을 촉진한다. 목단피의 미백효과를 알아보기 위하여 목단피 물추출물의 항산화 능력과 tyrosinase 활성 억제에 미치는 영향을 세포 모델계와 a cell-free 모델계에서 알아보았다. MDP의 라디칼 소거작용은 DPPH assay로 알아보았고, MDP는 높은 DPPH 라디칼 소거작용을 보여주었다. 세포내 tyrosinase 활성은 RT-PCR, 효소활성을 마우스 B16 세포내에서 측정함으로써 알아보았다. 24시간 목단피 물 추출물을 투여한 세포내에서 tyrosinase mRNA와 효소활성이 무처리군과 비교하여 현저히 감소하였다. 이와 같은 결과들은 항산화력이 높고, tyrosinase를 효과적으로 억제할 수 있는 목단피 물 추출물을 이용하여 melanin 생합성을 억제할 수 있는 미백 화장품 개발에 활용할 수 있는 가능성을 제시하고 있다.

• 한국작물학회지
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• 제60권3호
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• pp.366-373
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• 2015

This study was conducted to clarify the antimicrobial effect, antioxidant and tyrosinase inhibitory activities of the biological composition having the Phytolacca americana, and to enhance the natural materials utilization of foods and cosmetics. The antimicrobial activities of the different parts of P. americana were evaluated using the agar diffusion test. The antimicrobial activity of P. americana was relatively high in Malassezia furfur known as a skin fungi and Vibrio parahaemolyticus compared to Escherichia coli and Staphy-lococcus epidermidis. However, the antimicrobial activity in Vibrio parahaemolyticus did not show at all parts of P. americana. Both the DPPH radical scavenging activity and ABTS radical scavenging activity have been increased with the higher concentration of methanol extract. In particular, leaf extract of P. americana exhibited the highest activity both ABTS radical scavenging activity and DPPH radical scavenging activity. The nitrite scavenging activity was decreased when the pH was changed from pH 1.2 to pH 6.0. The highest nitrite scavenging activity was exhibited from the methanol extract of fruit, followed by root, stem, and leaf at pH 1.2. However, the nitrite scavenging activity at pH of 6.0 was not almost detected. All plant parts of P. americana showed tyrosinase inhibitory activity. The highest activity was found in the stem, and followed by root, leaf, and fruit in order. These tyrosinase inhibitory activity was progressively increased in a concentration-dependent manner. In this experiment on the methanol extracts of different organ from P. americana, we confirmed that the extract of P. americana showed potent tyrosinase inhibitory activity. Taken together, we conjectured that the P. americana had the potent biological activities, therefore this plant having various functional components could be a good material for development into source of natural food additives and cosmetics.