• Title/Summary/Keyword: two-scale grid

Search Result 130, Processing Time 0.031 seconds

Expression and Localization of Heat Shock Protein 70 in Frozen-Thawed IVF and Nuclear Transfrred Bovine Embryos

  • Park, Y.J;S.J Song;J.T Do;B.S Yoon;Kim, A.J;K.S Chung;Lee, H.T
    • Proceedings of the Korean Society of Embryo Transfer Conference
    • /
    • 2002.11a
    • /
    • pp.78-78
    • /
    • 2002
  • The role of heat shock proteins in shielding organism from environmental stress is illustrated by the large-scale synthesis of these protein by the organism studied to date. However, recent evidence also suggests an important role for heat shock protein in fertilization and early development of mammalian embryos. Effects of elevated in vitro temperature on in vitro produced bovine embryos were analysed in order to determine its impact on the expression of heat shock protein 70 (HSP70) by control and frozen-thawed after in vitro fertilization (IVF) or nuclear transfer (NT). The objective of this study was to assess the developmental potential in vitro produced embryos with using of the various containers and examined expression and localization of heat shock protein 70 after it's frozen -thawed. For the vitrification, in vitro produced embryos at 2 cell, 8 cell and blastocysts stage after IVF and NT were exposed the ethylene glycol 5.5 M freezing solution (EG 5.5) for 30 sec, loaded on each containers such EM grid, straw and cryo-loop and then immediately plunged into liquid nitrogen. Thawed embryos were serially diluted in sucrose solution, each for 1 min, and cultured in CRI-aa medium. Survival rates of the vitrification production were assessed by re-expanded, hatched blastocysts. There were no differences in the survival rates of IVF using EM grid, cryo-loop. However, survival rates by straw were relatively lower than other containers. Only, nuclear transferred embryos survived by using cryo-loop. After IVF or NT, in vitro matured bovine embryos 2 cell, 8 cell and blastocysts subjected to control and thawed conditions were analysed by semiquantitive reverse transcription polymerase chain reaction methods for hsp 70 mRNA expression. Results revealed the expression of hsp 70 mRNA were higher thawed embryos than control embryos. Immunocytochemistry used to localization the hsp70 protein in embryos. Two, 8-cell embryos derived under control condition was evenly distributed in the cytoplasm but appeared as aggregates in some embryos exposed frozen-thawed. However, under control condition, blastocysts displayed aggregate signal while Hsp70 in frozen-thawed blastocysts appeared to be more uniform in distribution.

  • PDF

A Comparison of 3D Reconstruction through the Passive and Pseudo-Active Acquisition of Images (수동 및 반자동 영상획득을 통한 3차원 공간복원의 비교)

  • Jeona, MiJeong;Kim, DuBeom;Chai, YoungHo
    • Journal of Broadcast Engineering
    • /
    • v.21 no.1
    • /
    • pp.3-10
    • /
    • 2016
  • In this paper, two reconstructed point cloud sets with the information of 3D features are analyzed. For a certain 3D reconstruction of the interior of a building, the first image set is taken from the sequential passive camera movement along the regular grid path and the second set is from the application of the laser scanning process. Matched key points over all images are obtained by the SIFT(Scale Invariant Feature Transformation) algorithm and are used for the registration of the point cloud data. The obtained results are point cloud number, average density of point cloud and the generating time for point cloud. Experimental results show the necessity of images from the additional sensors as well as the images from the camera for the more accurate 3D reconstruction of the interior of a building.

A Study on the Effect of Cumulus Parameterization and Microphysics on Ozone Simulations during Long-range Transport Process over Northeast Asia (동북아 장거리 수송 과정에서 적운 모수화 및 미세물리과정이 오존 모사농도에 미치는 영향 연구)

  • Kang, Jeong-Eon;Kim, Cheol-Hee
    • Journal of Korean Society for Atmospheric Environment
    • /
    • v.29 no.2
    • /
    • pp.135-151
    • /
    • 2013
  • This study has been carried out to analyze the sensitivity of ozone concentrations by employing different options of cumulus parameterization schemes (CPSs) and microphysics schemes in MM5 models. These sensitivity tests were applied to long-range transport case of higher ozone over Northeast Asia. Employed CPS schemes are Betts-Miller (BM), Grell (GR), Kain-Fritsch2 (KF2), Anthes-Kuo (AK), None scheme (grid scale physics only), and four microphysics used here are Simple ice, Reisner1, Reisner2, Schultz scheme in MM5. We chose two cases of high ozone long range transport case by employing both concentrations ozone level and backward trajectory model. The results showed that modeled ozone concentrations indicated about 10% differences among CPSs. Of the all options, GR and KF2 (for CPS), and Rersiner-1 and Resiner-2 (for microphysics) showed relatively good and stable variations against ensemble mean values. For both CPS and microphysics schemes, the difference of precipitation arising from different parameterization schemes was significant by itself, but the resultant ozone variations showed only marginal. But the cloud fraction differences arising from different parameterization schemes showed better correlation with ozone variations than precipitation differences, indicating that the photochemical ozone generation variations is more dominant by cloud fraction than wet removal process for high and long-ranged transported ozone cases over Northeast Asia.

The Characteristics of Seasonal Wind Fields around the Pohang Using Cluster Analysis and Detailed Meteorological Model (군집분석과 상세기상모델을 통한 포항지역 계절별 바람장 특성)

  • Jeong, Ju-Hee;Oh, In-Bo;Ko, Dae-Kwun;Kim, Yoo-Keun
    • Journal of Environmental Science International
    • /
    • v.20 no.6
    • /
    • pp.737-753
    • /
    • 2011
  • The typical characteristics of seasonal winds were studied around the Pohang using two-stage (average linkage then k-means) clustering technique based on u- and v-component wind at 850 hpa from 2004 to 2006 (obtained the Pohang station) and a high-resolution (0.5 km grid for the finest domain) WRF-UCM model along with an up-to-date detailed land use data during the most predominant pattern in each season. The clustering analysis identified statistically distinct wind patterns (7, 4, 5, and 3 clusters) representing each spring, summer, fall, and winter. During the spring, the prevailed pattern (80 days) showed weak upper northwesterly flow and late sea-breeze. Especially at night, land-breeze developed along the shoreline was converged around Yeongil Bay. The representative pattern (92 days) in summer was weak upper southerly flow and intensified sea-breeze combined with sea surface wind. In addition, convergence zone between the large scale background flow and well-developed land-breeze was transported around inland (industrial and residential areas). The predominant wind distribution (94 days) in fall was similar to that of spring showing weak upper-level flow and distinct sea-land breeze circulation. On the other hand, the wind pattern (117 days) of high frequency in winter showed upper northwesterly and surface westerly flows, which was no change in daily wind direction.

Accuracy Assessment of Ground Information Extracting Method from LiDAR Data (LiDAR자료의 지면정보 추출기법의 정확도 평가)

  • Choi, Yun-Woong;Choi, Nei-In;Lee, Joon-Whoan;Cho, Gi-Sung
    • Journal of Korean Society for Geospatial Information Science
    • /
    • v.14 no.4 s.38
    • /
    • pp.19-26
    • /
    • 2006
  • This study assessed the accuracies of the ground information extracting methods from the LiDAR data. Especially, it compared two kinds of method, one of them is using directly the raw LiDAR data which is point type vector data and the other is using changed data to DSM type as the normal grid type. The methods using Local Maxima and Entropy methods are applied as a former case, and for the other case, this study applies the method using edge detection with filtering and the generated reference surface by the mean filtering. Then, the accuracy assessment are performed with these results, DEM constructed manually and the error permitted limit in scale of digital map. As a results, each DEM mean errors of methods using edge detection with filtering, reference surface, Local Maxima and Entropy are 0.27m, 2.43m, 0.13m and 0.10m respectively. Hence, the method using entropy presented the highest accuracy. And an accuracy from a method directly using the raw LiDAR data has higher accuracy than the method using changed data to DSM type relatively.

  • PDF

Case Studies on Freezing Rain over the Korean Peninsula Using KLAPS (KLAPS를 이용한 한반도 어는비 사례 연구)

  • Kwon, Hui-Nae;Byun, Hi-Ryong;Park, Chang-Kyun
    • Atmosphere
    • /
    • v.25 no.3
    • /
    • pp.389-405
    • /
    • 2015
  • In this study, the occurrence circumstances of 3 cases (12 Jan 2006, 11 Jan 2008, 22 Feb 2009) when the freezing rain was observed at more than two observatories in a day with more than three times each observatory, were investigated. Following the advanced study about the same cases, we have tried to find more delicate differences in using the Korea Local Analysis and Prediction System (KLAPS; 5 km reanalysis data) that has the smallest grid scale at current situation. As results, three common characteristics are found: (1) Just before the occurrence of the freezing rain, the wind direction was consistently continuous and the wind speed was constant or gradually increased for at least 3 hr more. (2) Surface air temperature (Relative humidity) was respectively $3.08^{\circ}C$ (28.76%), $0.47^{\circ}C$ (50.07%) and $-3.60^{\circ}C$ (71.07%) 3 hr ago to break out the freezing rain. It means the freezing rain occurs in a wide range of atmospheric environments. However, the closer it got to the occurrence time of the freezing rain, the closer the surface air temperature was to $0^{\circ}C$, and the bigger the humidity of the surface air was. (3) The liquid precipitation formed in the upper atmosphere, met a cold advection bellower than 950 hPa level and suspected to be changed to the super-cooled condition.

Effects of Facing Types and Construction Procedures on the Stability of Reinforced Earth Wall (전면벽 및 축조순서가 보강토옹벽의 안정성에 미치는 영향)

  • Lim Yu-Jin;Jung Jong-Hong;Park Young-Ho
    • Journal of the Korean Geotechnical Society
    • /
    • v.20 no.7
    • /
    • pp.119-126
    • /
    • 2004
  • A small-scale reinforced earth wall was constructed in a laboratory to investigate the effect of wall rigidity and of construction sequence on the wall. A full continuous wall facing and a discrete wall facing were designed and constructed for tests. These two different facing systems should adapt different construction procedures due to their different facing shapes. The model wall was built with geo-grid reinforcement, sand, and facings on rigid surface. The model wall was instrumented with earth pressure gages, LVDTs, and strain gages. The experimental results have shown differences in wall behavior related to construction sequence and types of wall facing. It is found in this study that the reinforced earth wall built with full continuous facing is safer than the reinforced earth wall built with the discrete wall facing.

Effects of diffraction in regular head waves on added resistance and wake using CFD

  • Lee, Cheol-Min;Park, Sung-Chul;Yu, Jin-Won;Choi, Jung-Eun;Lee, Inwon
    • International Journal of Naval Architecture and Ocean Engineering
    • /
    • v.11 no.2
    • /
    • pp.736-749
    • /
    • 2019
  • This paper employs computational tools to investigate the diffraction effects in regular head waves on the added resistance and wake on the propeller plane. The objective ships are a 66,000 DWT bulk carrier and a 3,600 TEU container ship. Fixed and free to heave and pitch conditions at design speed have been taken into account. Two-phase unsteady Reynolds averaged Navier-Stokes equations have been solved using the finite volume method; and a realizable k-ε model has been applied for the turbulent closure. The free surface is obtained by solving a VOF equation. The computations are carried out at the same scale of the model tests. Grid and numerical wave damping zones are applied to remove unwanted wave reflection at the boundaries. The computational results are analyzed using the Fourier series. The added resistances in waves at the free condition are higher than those at the fixed condition, which are nearly constant for all wavelengths. The wake velocity in waves is higher than that in calm water, and is accelerated where the wave crest locates on the propeller plane. When the vertical motion at the stern goes upward, the wake velocity also accelerated.

Assessment of performance for Output Power Control of Wind Turbine using Energy Storage System (에너지저장장치를 이용한 풍력발전 출력 제어 성능 평가)

  • Hong, Jong-Seok;Choi, Chang-Ho;Lee, Joo-Yeon;Kim, Jae-Chul
    • The Transactions of the Korean Institute of Electrical Engineers P
    • /
    • v.63 no.4
    • /
    • pp.254-259
    • /
    • 2014
  • In this paper, we describe construction of a wind stabilization demo-site and effects of output power control of wind turbines for suppression of ramp rate using ESS (Energy Storage System). It is difficult to control the output power of distributed generator such as wind turbine which of variation is very large. If the large capacity wind farm be interconnected into power system may cause blackout due to Power Quality. For these reasons, the international standards such as Grid-Code is limited to less than 10 [%/min] of renewable energy ramp rate. The case of Korea, government actively conducts propagating large-scale renewable energy for green growth policy, to interconnecting more renewable energy into power system is necessary for stabilization technology. For these reasons, the POSCO consortium has constructed a wind stabilization demo-site that is configured as 500 [kWh] battery energy storage systems can output up to 3 [C-Rate] and two wind turbines rated 750 [kW]. In POSCO consortium, which implements various methods stabilizing output power of wind turbine such as smoothing, section firming and ramp control, we derive the results of long-term demonstration that can be controlled to satisfy to the international standard about ramp rate [%/kW] of wind turbine output power.

NUMERICAL APPROACH FOR QUANTIFICATION OF SELFWASTAGE PHENOMENA IN SODIUM-COOLED FAST REACTOR

  • JANG, SUNGHYON;TAKATA, TAKASHI;YAMAGUCHI, AKIRA;UCHIBORI, AKIHIRO;KURIHARA, AKIKAZU;OHSHIMA, HIROYUKI
    • Nuclear Engineering and Technology
    • /
    • v.47 no.6
    • /
    • pp.700-711
    • /
    • 2015
  • Sodium-cooled fast breeder reactors use liquid sodium as a moderator and coolant to transfer heat from the reactor core. The main hazard associated with sodium is its rapid reaction with water. Sodium-water reaction (SWR) takes place when water or vapor leak into the sodium side through a crack on a heat-transfer tube in a steam generator. If the SWR continues for some time, the SWR will damage the surface of the defective area, causing it to enlarge. This self-enlargement of the crack is called "self-wastage phenomena." A stepwise numerical evaluation model of the self-wastage phenomena was devised using a computational code of multicomponent multiphase flow involving a sodium-water chemical reaction: sodiumwater reaction analysis physics of interdisciplinary multiphase flow (SERAPHIM). The temperature of gas mixture and the concentration of NaOH at the surface of the tube wall are obtained by a numerical calculation using SERAPHIM. Averaged thermophysical properties are used to assess the local wastage depth at the tube surface. By reflecting the wastage depth to the computational grid, the self-wastage phenomena are evaluated. A two-dimensional benchmark analysis of an SWAT (Sodium-Water reAction Test rig) experiment is carried out to evaluate the feasibility of the numerical model. Numerical results show that the geometry and scale of enlarged cracks show good agreement with the experimental result. Enlarged cracks appear to taper inward to a significantly smaller opening on the inside of the tube wall. The enlarged outer diameter of the crack is 4.72 mm, which shows good agreement with the experimental data (4.96 mm).