• Tuberculosis and Respiratory Diseases
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• 제45권4호
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• pp.714-722
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• 1998

연구배경: Rifampicin은 항결핵 단기요법의 근간이 되는 약제로 rifampicin 내성용 다제내성의 지표이기도 하다. rpoB유전자는 rifampicin이 결합하여 약리작용을 나타내는 RNA polymerase의 $\beta$-subunit을 coding하는 유전자이며 rifampicin내성 결핵균의 약 96%에서 돌연변이가 관찰된다. 그러므로 rifampicin내성결핵을 빠르게 진단할 수 있는 유용한 방법을 확인하기 위하여 PCR-line probe법과 PCR-SSCP법을 이용하여 다음의 연구를 시행하였다. 방 법: 대한 결핵연구원의 약제감수성검사상 rifampicin 내성인 결핵균주 33예와 감수성인 결핵균주 12예를 대상으로 하였다. 각각 배양균 집락에서 DNA를 분리한 후, PCR-line probe법과 PCR-SSCP법을 이용하여 rifampicin 내성 여부를 단일맹검적으로 검사하였고, 이를 약제감수성검사 결과와 비교하였다. 결 과: PCR-line probe법으로는 내성균주 33예중 27예(81.8%)에서 rpoB 유전자의 돌연변이를 확인할 수 있었고, 감수성균주 12예는 모두 rpoB 유전자의 돌연변이는 없었다. PCR-SSCP 법으로는 내성균주 33예중 23예(69.7%)에서 rpoB 유전자의 돌연변이를 확인할 수 있었고, 감수성균주 12예는 모두 rpoB 유전자의 돌연변이는 없었다. Rifampicin내성균주에서 rpoB 유전자 돌연변이 검출율의 PCR-line probe 법과 PCR-SSCP법간의 차이는 없었다 (p=0.25). 결 론: PCR-line probe법은 PCR-SSCP법과 더불어 rifampicin 내성을 조기에 진단할 수 있는 좋은 방법으로 사료되며, 전통적인 약제감수성검사 결과를 기다릴 수 없는 국한된 환자에게 유용한 진단법으로 생각된다.

• 대한전자공학회논문지
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• 제8권6호
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• pp.1-7
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• 1971

본 연구는 액체내의 초음파강도측정법에 관한 것으로 반사판의 양측에 흡음특질로 포위된 thermocouple의 감온점을 첨부한 구조를 가진 probe에 직접 받은 음파와 반사판을 투과한 음파에 의하여 상승된 감온점의 온도차를 검출하여 초음파강도를 온정하였다. 이것은 액온의 영향과 time constant를 적게하고 초음파 진행을 고려한 국소 강도를 간편하게 측정할 수 있는 공업용 계기제작을 위한 기초연구로서 시도한 것이다.

• Archives of Pharmacal Research
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• 제9권3호
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• pp.139-144
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• 1986

The solubilization sites of aniline and its derivatives in micelles were investigated with fluorescence probe technique. The fluorescence probes employed in this study are 12-(9-anthroyl) stearic acid (AS) and 2-p-toluidinylnaphthalene-6-sulfonate (TNS) which are incorporated in the interior of the micelle and attaced to its surface, respectively. As these two probes were effectively quenched by aniline and its surface, respectively. As these two probes were effectively quenched by aniline and its derivatives, the modified Stern-Volmer relationship in micellar system could be applicable to estimate the partition coefficient, $K_{p}$ of the solubilizate between aqueous and micellar phase. Because $K_{p}$ derived by this method reflects the relative proximity of the fluorophore to the quencher, the ratio of $K_{p}$ in the surface area to that in the interior of the micelle is interpreted in terms of the relative location of the solubilizate in micellar aggregate. The results show that the solubilizates are not located in a definite position but distributed in the multiple-sites of the micelle. The solubilization sites of the solubilizates in the icelle are dependent on their structures. As the solubilizate has more numbers of N-substituents of aniline and more numbers, of carbon in the substituent, it tends to incorporate in the interior of the micelle more effectively.

• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.248-255
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• 2012

In order to develop a protocol to quantify cyanobacteria and Microcystis simultaneously, the primers and probe were designed from the conserved regions of 16S rRNA gene sequences of cyanobacteria and Microcystis, respectively. Probe match analysis of the Ribosomal Database Project showed that the primers matched with over 97% of cyanobacterial 16S rRNA genes, indicating these can be used to amplify cyanobacteria specifically. The TaqMan probe, which is located between two primers, matched with 98.2% of sequences in genus GpXI, in which most Microcystis strains are included. The numbers of cyanobacterial genes were estimated with the emission of SYBR Green from the amplicons with two primers, whereas those of Microcystis spp. were measured from the fluorescence of CAL Fluor Gold 540 emitted by exonuclease activity of Taq DNA polymerase in amplification. It is expected that this method enhances the accuracy and reduces the time to count cyanobacteria and potential toxigenic Microcystis spp. in aquatic environmental samples.

• 한국인터넷방송통신학회논문지
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• 제19권3호
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• pp.59-65
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• 2019

마그네틱 프로브와 노이즈 소스의 근거리장에 금속 차폐막이 놓여있을 때 차폐막 두께에 따른 EMI 차폐효과를 분석하였다. 노이즈 소스로는 마이크로스트립 라인을 이용하였으며, 금속 차폐막 재료로는 그래파이트와 페라이트를 선정하였다. 마그네틱 프로브는 IEC 61967-6 방식을 적용하여 마그네틱 프로브를 이용한 전자기 방사 측정 방법을 사용하였다. 마이크로스트립 라인과 마그네틱 프로브 사이의 투과계수를 분석하였고, 둘 사이의 거리는 단일 차폐막일 경우 1 mm, 다중 차폐막일 경우 5 mm로 수행하였다. 차폐막의 두께는 5 um, 10 um, 30 um, 50 um 으로 변화시켰다. 주파수를 150 kHz에서 1 GHz까지 변화시켰을 경우 최대 44.9 dB의 SE(Shielding Effectiveness)를 얻었다.

• International Journal of Precision Engineering and Manufacturing
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• 제6권3호
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• pp.45-50
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• 2005

This paper proposed an efficient manufacturing system using the OMM (on-machine measurement) system. The OMM system is software-based 3D modeler for inspection on machine, and it is interfaced with machine tools via RS232C. The software is composed of two inspection modules; one is touch probe operating module, and the other is laser displacement sensor operating module. The module for touch probe needs the inspection feature extracted from CAD data. The touch probe moves to workpiece by three operating modes as follows: manual, general and automatic mode. The operating module of the laser displacement sensor is used to inspect profiles and very small holes. An advantage of this inspection method is the ability to execute on-line inspection during machining or afterward. The efficiency of proposed system which can predict and define the machining errors of each process was verified, so the developed system was applied to inspect a mold-base (cavity, core).

• International Journal of Naval Architecture and Ocean Engineering
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• 제12권1호
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• pp.578-595
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• 2020

Submarine pressure hulls must withstand high hydraulic pressure and be free of defects. To improve the precision of defect detection, we herein examined different probes for optimal defect assessment by applying the Phased Array Ultrasonic Testing (PAUT) method. Two sets of probe design parameters were selected by considering pressure hull characteristics and analyzed through modeling. PAUT probes were applied, and defect assessment results were compared based on ultrasonic signals of various simulated defects in specimens designed to be the same as actual pressure hulls. The final selected design parameters for the submarine probe, which were designed to minimize the grating lobe of wave interference effect and improve the ultrasonic resolution of pressure hull welds, were identified through the experiment. The improvement in the probe's ability to detect defects in a pressure hull was verified. Furthermore, the accuracy of defect length measurement was improved, enhancing the applicability of the technique.

• Interdisciplinary Bio Central
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• 제4권2호
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• pp.5.1-5.9
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• 2012

Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.

• 한국정밀공학회지
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• 제20권2호
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• pp.176-183
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• 2003

The paper presents a feature recognition and segmentation method for surface approximation in reverse engineering. Efficient digitizing plays an important role in constructing a computational surface model from a physical part-surface without its CAD model on hand. Depending on its measuring source (e.g., touch probe or structured light), each digitizing method has its own strengths and weaknesses in terms of speed and accuracy. The final goal of the research focuses on an integration of two different digitizing methods: measuring by the structured light and that by the touch probe. Gathering bulk of digitized points (j.e., cloud-of-points) by use of a laser scanning system, we construct a coarse surface model directly from the cloud-of-points, followed by the segmentation process where we utilize the z-map filleting & differencing to trace out feature boundary curves. The feature boundary curves and the approximate surface model could be inputs to further digitizing by a scanning touch probe. Finally, more accurate measuring points within the boundary curves can be obtained to construct a finer surface model.

• 전자공학회논문지D
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• 제35D권10호
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• pp.7-12
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• 1998

본 논문에서는 다양한 물질에 접촉해 있는 여러 형태의 동축선 끝단에서의 전파반사를 FDTD 방법으로 해석하였다. 3차원의 해석 구조를 2차원 원통 좌표계로 변환하여 매우 효율적으로 계산할 수 있었다. FDTD 계산결과를 통하여, 주파수가 높아지거나 또는 동축선로의 직경이 증가할 때 동축선 끝단에서의 반사크기가 줄어든다는 것을 알았다. 이 방법을 적용하여 다층 구조에서의 전파반사도 해석하였으며, 측정결과와 이론식을 이용하여 본 해석 방법을 검증하였다.