• Tuberculosis and Respiratory Diseases
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• v.45 no.4
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• pp.714-722
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• 1998

Background: Rifampicin (RFP) is a key component of the antituberculous short-course chemotherapy and the RFP resistance is a marker of multi-drug resistant (MDR) tuberculosis. RPoB gene encodes the $\beta$-subunit of RNA polymerase of M. tuberculosis which is the target of RFP. And the mutations of rpoB gene have been found in about 96% of rifampicin resistant clinical isolates of M. tuberculosis. So in order to find a rapid and clinically useful diagnostic method in identifying the RFP resistance, we compared the PCR -line probe method with PCR-SSCP for the detection of the rpoB gene mutation in cultured M. tuberculosis. Methods: 45 clinical isolates were collected from patients who visited Sung Kyun Kwan University Hospital. The RFP susceptibility test was referred to the referral laboratory of the Korean Tuberculosis Institute. 33 were rifampicin resistant and 12 were rifampicin susceptible. The susceptibility results were compared with the results of the PCR-BSCP and PCR-line probe method. Results: We could find rpoB mutations in 27/33(81.8%) RFP-resistant strains by PCR-line probe method, and in 23/33 (69.7%) by PCR-SSCP and there was no significant difference between two methods. There was no mutation in rifampicinn susceptible strains by both methods. Conclusion: PCR-line probe method would be a rapid, sensitive and specific method for the detection of rifampicin resistant Mycobacterium tuberculosis.

• Journal of the Korean Institute of Telematics and Electronics
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• v.8 no.6
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• pp.1-7
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• 1971

This paper describes concerning the method of measurement for the intensity of ultrasonics in liquid. The probe which is added two sensive point of thermocople which is surrounded by the absorbed material of sonic wave on the both side of the refleftite plate have measured the intensity of ultrasonics indirectly by the difference of the temperature of two sensive point which had raised the temperature by the sonic wave and its reflective wave which transmitted the ultrasonic energy. This minify the influence from the temparature of liquid and time constant. Consequently, this is the basic industrial method which is with in the bounds of possibility on the simple measurement of the intensity of the local ultrasonics concerning direction of its propagation.

• Archives of Pharmacal Research
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• v.9 no.3
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• pp.139-144
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• 1986

The solubilization sites of aniline and its derivatives in micelles were investigated with fluorescence probe technique. The fluorescence probes employed in this study are 12-(9-anthroyl) stearic acid (AS) and 2-p-toluidinylnaphthalene-6-sulfonate (TNS) which are incorporated in the interior of the micelle and attaced to its surface, respectively. As these two probes were effectively quenched by aniline and its surface, respectively. As these two probes were effectively quenched by aniline and its derivatives, the modified Stern-Volmer relationship in micellar system could be applicable to estimate the partition coefficient, $K_{p}$ of the solubilizate between aqueous and micellar phase. Because $K_{p}$ derived by this method reflects the relative proximity of the fluorophore to the quencher, the ratio of $K_{p}$ in the surface area to that in the interior of the micelle is interpreted in terms of the relative location of the solubilizate in micellar aggregate. The results show that the solubilizates are not located in a definite position but distributed in the multiple-sites of the micelle. The solubilization sites of the solubilizates in the icelle are dependent on their structures. As the solubilizate has more numbers of N-substituents of aniline and more numbers, of carbon in the substituent, it tends to incorporate in the interior of the micelle more effectively.

• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.248-255
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• 2012

In order to develop a protocol to quantify cyanobacteria and Microcystis simultaneously, the primers and probe were designed from the conserved regions of 16S rRNA gene sequences of cyanobacteria and Microcystis, respectively. Probe match analysis of the Ribosomal Database Project showed that the primers matched with over 97% of cyanobacterial 16S rRNA genes, indicating these can be used to amplify cyanobacteria specifically. The TaqMan probe, which is located between two primers, matched with 98.2% of sequences in genus GpXI, in which most Microcystis strains are included. The numbers of cyanobacterial genes were estimated with the emission of SYBR Green from the amplicons with two primers, whereas those of Microcystis spp. were measured from the fluorescence of CAL Fluor Gold 540 emitted by exonuclease activity of Taq DNA polymerase in amplification. It is expected that this method enhances the accuracy and reduces the time to count cyanobacteria and potential toxigenic Microcystis spp. in aquatic environmental samples.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.19 no.3
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• pp.59-65
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• 2019

The EMI shielding effectiveness of the shielding layer thickness was analyzed when the metal shielding layer was placed in the near field of the magnetic probe and the noise source. Microstrip lines were used as noise source, and graphite and ferrite were selected as metal shielding materials. The magnetic probe uses the electromagnetic radiation measurement method using the magnetic probe by applying the IEC 61967-6 method. The transmission coefficient between the microstrip line and the magnetic probe was analyzed. The distance between the two was 1 mm for a single shielding layer and 5 mm for a multiple shielding layer. The thickness of the shielding layer was changed to 5 um, 10 um, 30 um, and 50 um. When the frequency was changed from 150 kHz to 1 GHz, a maximum shielding effectiveness (SE) of 44.9 dB was obtained.

• International Journal of Precision Engineering and Manufacturing
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• v.6 no.3
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• pp.45-50
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• 2005

This paper proposed an efficient manufacturing system using the OMM (on-machine measurement) system. The OMM system is software-based 3D modeler for inspection on machine, and it is interfaced with machine tools via RS232C. The software is composed of two inspection modules; one is touch probe operating module, and the other is laser displacement sensor operating module. The module for touch probe needs the inspection feature extracted from CAD data. The touch probe moves to workpiece by three operating modes as follows: manual, general and automatic mode. The operating module of the laser displacement sensor is used to inspect profiles and very small holes. An advantage of this inspection method is the ability to execute on-line inspection during machining or afterward. The efficiency of proposed system which can predict and define the machining errors of each process was verified, so the developed system was applied to inspect a mold-base (cavity, core).

• International Journal of Naval Architecture and Ocean Engineering
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• v.12 no.1
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• pp.578-595
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• 2020

Submarine pressure hulls must withstand high hydraulic pressure and be free of defects. To improve the precision of defect detection, we herein examined different probes for optimal defect assessment by applying the Phased Array Ultrasonic Testing (PAUT) method. Two sets of probe design parameters were selected by considering pressure hull characteristics and analyzed through modeling. PAUT probes were applied, and defect assessment results were compared based on ultrasonic signals of various simulated defects in specimens designed to be the same as actual pressure hulls. The final selected design parameters for the submarine probe, which were designed to minimize the grating lobe of wave interference effect and improve the ultrasonic resolution of pressure hull welds, were identified through the experiment. The improvement in the probe's ability to detect defects in a pressure hull was verified. Furthermore, the accuracy of defect length measurement was improved, enhancing the applicability of the technique.

• Interdisciplinary Bio Central
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• v.4 no.2
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• pp.5.1-5.9
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• 2012

Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.2
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• pp.176-183
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• 2003

The paper presents a feature recognition and segmentation method for surface approximation in reverse engineering. Efficient digitizing plays an important role in constructing a computational surface model from a physical part-surface without its CAD model on hand. Depending on its measuring source (e.g., touch probe or structured light), each digitizing method has its own strengths and weaknesses in terms of speed and accuracy. The final goal of the research focuses on an integration of two different digitizing methods: measuring by the structured light and that by the touch probe. Gathering bulk of digitized points (j.e., cloud-of-points) by use of a laser scanning system, we construct a coarse surface model directly from the cloud-of-points, followed by the segmentation process where we utilize the z-map filleting & differencing to trace out feature boundary curves. The feature boundary curves and the approximate surface model could be inputs to further digitizing by a scanning touch probe. Finally, more accurate measuring points within the boundary curves can be obtained to construct a finer surface model.

• Journal of the Korean Institute of Telematics and Electronics D
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• v.35D no.10
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• pp.7-12
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• 1998

The wave reflections from several types of open-ended coaxial probes contacted on the various materials have been analyzed precisely by using the finite-difference time-domain(FDTD) technique in this paper. Due to the coordinate transformation from three-dimension to two-dimension, the computation was performed very efficiently. It was found that the reflection from an open-ended coaxial probe reduces as frequency or diameter of a coaxial line increases. The reflections from multi-layered media were also analyzed by the FDTD method. This analysis technique was verified by comparison with measurements and theoretical computations.