• Title/Summary/Keyword: two-hybrid

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Characteristics of Settlement and Scour of Square-Shaped Hybrid Reef according to Placement Types (배치 형태에 따른 사각형 복합 인공어초의 침하 및 세굴 특성)

  • Yun, Daeho;Kim, Yuntae
    • Journal of the Korean Geosynthetics Society
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    • v.16 no.2
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    • pp.1-10
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    • 2017
  • This study carried out several laboratory tests such as water tank settlement test and two-dimensional water flow test to investigate settlement and scour characteristics of a square-shaped hybrid reef which was reinforced with geogrid. In addition, numerical analysis was also performed to find out scour characteristics of the square-shaped hybrid reef with different placement types (type A; straight placement type, type B; zigzag placement type). Laboratory test results indicated that settlement and scouring depth significantly reduced in hybrid reefs reinforced with geogrid, compared with those without reinforcement. Two-dimentional water flow tests and numerical test results also showed that scouring depth and flow velocity of placement type A more decreased than those of type B.

Architectures and Connection Probabilities forWireless Ad Hoc and Hybrid Communication Networks

  • Chen, Jeng-Hong;Lindsey, William C.
    • Journal of Communications and Networks
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    • v.4 no.3
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    • pp.161-169
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    • 2002
  • Ad hoc wireless networks involving large populations of scattered communication nodes will play a key role in the development of low power, high capacity, interactive, multimedia communication networks. Such networks must support arbitrary network connections and provide coverage anywhere and anytime. This paper partitions such arbitrarily connected network architectures into three distinct groups, identifies the associated dual network architectures and counts the number of network architectures assuming there exist N network nodes. Connectivity between network nodes is characterized as a random event. Defining the link availability P as the probability that two arbitrary network nodes in an ad hoc network are directly connected, the network connection probability $ \integral_n$(p) that any two network nodes will be directly or indirectly connected is derived. The network connection probability $ \integral_n$(p) is evaluated and graphically demonstrated as a function of p and N. It is shown that ad hoc wireless networks containing a large number of network nodes possesses the same network connectivity performance as does a fixed network, i.e., for p>0, $lim_{N\to\infty} Integral_n(p)$ = 1. Furthermore, by cooperating with fixed networks, the ad hoc network connection probability is used to derive the global network connection probability for hybrid networks. These probabilities serve to characterize network connectivity performance for users of wireless ad hoc and hybrid networks, e.g., IEEE 802.11, IEEE 802.15, IEEE 1394-95, ETSI BRAN HIPERLAN, Bluetooth, wireless ATM and the world wide web (WWW).

Hydrogen Evolution from Biological Protein Photosystem I and Semiconductor BiVO4 Driven by Z-Schematic Electron Transfer

  • Shin, Seonae;Kim, Younghye;Nam, Ki Tae
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.251.2-251.2
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    • 2013
  • Natural photosynthesis utilizes two proteins, photosystem I and photosystem II, to efficiently oxidize water and reduce NADP+ to NADPH. Artificial photosynthesis which mimics this process achieve water splitting through a two-step Z-schematic water splitting process using man-made synthetic materials for hydrogen fuel production. In this study, Z-scheme system was achieved from the hybrid materials which composed of hydrogen production part as photosystem I protein and water oxidizing part as semiconductor BiVO4. Utilizing photosystem I as the hydrogen evolving part overcomes the problems of existing hydrogen evolving p-type semiconductors such as water instability, expensive cost, few available choices and poor red light (>600 nm) absorbance. Some problems of photosystem II, oxygen evolving part of natural photosynthesis, such as demanding isolation process and D1 photo-damage can also be solved by utilizing BiVO4 as the oxygen evolving part. Preceding research has not suggested any protein-inorganic-hybrid Z-scheme composed of both materials from natural photosynthesis and artificial photosynthesis. In this study, to realize this Z-schematic electron transfer, diffusion step of electron carrier, which usually degrades natural photosynthesis efficiency, was eliminated. Instead, BiVO4 and Pt-photosystem I were all linked together by the mediator gold. Synthesized all-solid-state hybrid materials show enhanced hydrogen evolution ability directly from water when illuminated with visible light.

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A hybrid neutronics method with novel fission diffusion synthetic acceleration for criticality calculations

  • Jiahao Chen;Jason Hou;Kostadin Ivanov
    • Nuclear Engineering and Technology
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    • v.55 no.4
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    • pp.1428-1438
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    • 2023
  • A novel Fission Diffusion Synthetic Acceleration (FDSA) method is developed and implemented as a part of a hybrid neutronics method for source convergence acceleration and variance reduction in Monte Carlo (MC) criticality calculations. The acceleration of the MC calculation stems from constructing a synthetic operator and solving a low-order problem using information obtained from previous MC calculations. By applying the P1 approximation, two correction terms, one for the scalar flux and the other for the current, can be solved in the low-order problem and applied to the transport solution. A variety of one-dimensional (1-D) and two-dimensional (2-D) numerical tests are constructed to demonstrate the performance of FDSA in comparison with the standalone MC method and the coupled MC and Coarse Mesh Finite Difference (MC-CMFD) method on both intended purposes. The comparison results show that the acceleration by a factor of 3-10 can be expected for source convergence and the reduction in MC variance is comparable to CMFD in both slab and full core geometries, although the effectiveness of such hybrid methods is limited to systems with small dominance ratios.

Natural Hybrids between Pungitius sinensis sinensis and P. sinensis kaibarae (가시고기(Pungitius sinensis sinensis)와 잔가시고기(P. sinensis kaibarae) 사이의 자연잡종(自然雜種))

  • Chae, Byung-Soo;Yang, Hong-Jun
    • Korean Journal of Ichthyology
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    • v.2 no.1
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    • pp.88-94
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    • 1990
  • Natural hybrids between Pungitius sisensis sinensis and P. sinensis kaibarae were found in their sympatric area, a streamlet below Chibyon Reservoir in Kangnung city, Kangwondo, Korea. P. sinensis sinensis and P. sinensis kaibarae showed the same electropherograms as reported previously. However, about 10% of the specimens had peculiar banding patterns and they seemed to be hybrid between the two subspecies. They are divided further into two groups: sinensis-type and kaibarae-type hybrid. Two morphometric characters, snout length and depth of caudal peduncle, indicated the hybrid's property well. The color of testis of P. sinensis sinensis and P. sinensis kaibarae was grey and black, respectively, but that of hybrid was intermediate. The problem of reproductive isolation between the two groups raised by the finding of their hybrids was discussed.

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An Effcient Two-Level Hybrid Signature File Method for Large Text Databases (대용량 텍스트 데이터베이스를 위한 효율적인 2단계 합성 요약 화일 방법)

  • Yoo, Jae-Soo;Gang, Hyeong-Il
    • The Transactions of the Korea Information Processing Society
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    • v.4 no.4
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    • pp.923-932
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    • 1997
  • In this paper, we propose a two-level hybrid signature file method(THM) to dffciently deal with large txt databases that use a term discrimination concept.In addition, we apply Yoo's clustering scheme to the two-level hybeid signature file method. The clustering schme groups similar signatures together according to the similarity of the highly discriminatiory tems so that we may achive better performance on retrival. The space-time ana-lyhtical model of the proposed two-level hybrid method is provided. Based on the analytical model and experiments, we compare it with the exsting methods, i.e. the bit-sliced method(BM), the-level method(TM), and the hybrid method(HM). As a result, we show that THM achives the best retrival performance in a large database with 100,000 records when the mumber fo matching records is less than 160.

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Construction of the Detection System of Endocrine Disrupters using Yeast Two-Hybrid System with Human Estrogen Receptor ligand Binding Domain and Co-activators (Human Estrogen Receptor Ligand Binding Domain (hER LBD)과 Co-activator로 구성된 효모 Two-Hybrid System을 이용한 내분비계장애물질 검출계의 구축)

  • 이행석;조은민;류재천
    • Environmental Mutagens and Carcinogens
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    • v.22 no.3
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    • pp.175-182
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    • 2002
  • Endocrine disruptors (EDs) are the chemicals that affect endocrine systems through activation or inhibition of steroid hormone response. It is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. The key targets of EDs are nuclear hormone receptors, which bind to steroid hormones and regulate their gene transcription. We constructed a co-expression system of Gal4p DNA binding domain (DBD)- ligand binding domain of human estrogen receptor $\alpha$ or $\beta$, and Gal4p transactivation domain (TAD)-co-activator AIB-1, SRC-1 or TIF-2 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and Gal4p binding site (GAL4 upstream activating sequence, GAL4$_{UAS}$). Expression of this reporter gene was dependent on the presence of estrogen or EDs in the culture medium. We found that the two-hybrid system with combination of the hER$\beta$ LBD and co-activator SRC-1 was most effective in the xenoestrogen-dependent induction of reporter activity. The extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. The data in this research demonstrated that the yeast detection system using steroid hormone receptor and co-activator is a useful tool for identifying chemicals that interact with steroid receptors.s.

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Direct Interaction of KIF5s and Actin-Based Transport Motor, Myo9s (KIF5s와 직접 결합하는 액틴 결합 운동단백질 Myo9s의 규명)

  • Seog, Dae-Hyun
    • Journal of Life Science
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    • v.21 no.8
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    • pp.1076-1082
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    • 2011
  • Microtubule-based kinesin motor proteins are used for long-range vesicular transport. KIF5s (KIF5A, KIF5B and KIF5C) mediate the transport of various membranous vesicles along microtubules, but the mechanism behind how they recognize and bind to a specific cargo has not yet been completely elucidated. To identify the interaction protein for KIF5B, yeast two-hybrid screening was performed and a specific interaction with the unconventional myosin Myo9b, an actin-based vesicle transport motor, was found. The GTPase-activating protein (GAP) domain of Myo9s was essential for interaction with KIF5B in the yeast two-hybrid assay. Myo9b bound to the carboxyl-terminal region of KIF5B and to other KIF5 members. In addition, glutathione S-transferase (GST) pull-downs showed that Myo9s specifically interact to the complete Kinesin-I complex. An antibody to KIF5B specifically co-immunoprecipitated KIF5B associated with Myo9s from mouse brain extracts. These results suggest that kinesin-I motor protein interacts directly with actin-based motor proteins in the cell.

Interaction Study of Soybean mosaic virus Proteins with Soybean Proteins using the Yeast-Two Hybrid System

  • Seo, Jang-Kyun;Hwang, Sung-Hyun;Kang, Sung-Hwan;Choi, Hong-Soo;Lee, Su-Heon;Sohn, Seong-Han;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • v.23 no.4
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    • pp.281-286
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    • 2007
  • Interactions between viral proteins and host proteins are essential for virus replication. Especially, translation of viral genes completely depends on the host machinery. In potyviruses, interactions of genome-linked viral protein (VPg) with host translation factors including eIF4E, eIF(iso)4E, and poly(A)-binding protein (PABP) has previously been characterized. In this study, we investigated interactions between Soybean mosaic virus (SMV) viral proteins and host translation factors by yeast two-hybrid system. SMV VPg interacted with eIF4E, eIF(iso)4E, and PABP in yeast two-hybrid system, while SMV helper component proteinase (HC-pro) interacted with neither of those proteins. The interaction between SMV NIb and PABP was also detected. These results are consistent with those reported previously in other potyviruses. Interestingly, we found reproducible and specific interactions between SMV coat protein (CP) and PABP. Deletion analysis showed that the region of CP comprising amino acids 116 to 206 and the region of PABP comprising amino acids 520 to 580 are involved in CP/PABP interactions. Soybean library screening with SMV NIb by yeast two-hybrid assay also identified several soybean proteins including chlorophyll a/b binding preprotein, photo-system I-N subunit, ribulose 1,5-biphosphate carboxylase, ST-LSI protein, translation initiation factor 1, TIR-NBS type R protein, RNA binding protein, ubiquitin, and LRR protein kinase. Altogether, these results suggest that potyviral replicase may comprise a multi-protein complex with PABP, CP, and other host factors.

Comparing the performance of two hybrid deterministic/Monte Carlo transport codes in shielding calculations of a spent fuel storage cask

  • Lai, Po-Chen;Huang, Yu-Shiang;Sheu, Rong-Jiun
    • Nuclear Engineering and Technology
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    • v.51 no.8
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    • pp.2018-2025
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    • 2019
  • This study systematically compared two hybrid deterministic/Monte Carlo transport codes, ADVANTG/MCNP and MAVRIC, in solving a difficult shielding problem for a real-world spent fuel storage cask. Both hybrid codes were developed based on the consistent adjoint driven importance sampling (CADIS) methodology but with different implementations. The dose rate distributions on the cask surface were of primary interest and their predicted results were compared with each other and with a straightforward MCNP calculation as a baseline case. Forward-Weighted CADIS was applied for optimization toward uniform statistical uncertainties for all tallies on the cask surface. Both ADVANTG/MCNP and MAVRIC achieved substantial improvements in overall computational efficiencies, especially for gamma-ray transport. Compared with the continuous-energy ADVANTG/MCNP calculations, the coarse-group MAVRIC calculations underestimated the neutron dose rates on the cask's side surface by an approximate factor of two and slightly overestimated the dose rates on the cask's top and side surfaces for fuel gamma and hardware gamma sources because of the impact of multigroup approximation. The fine-group MAVRIC calculations improved to a certain extent and the addition of continuous-energy treatment to the Monte Carlo code in the latest MAVRIC sequence greatly reduced these discrepancies. For the two continuous-energy calculations of ADVANTG/MCNP and MAVRIC, a remaining difference of approximately 30% between the neutron dose rates on the cask's side surface resulted from inconsistent use of thermal scattering treatment of hydrogen in concrete.