• Proceedings of the Korean Society of Precision Engineering Conference
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• 2008.06a
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• pp.571-572
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• 2008

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.149.1-149.1
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• 2008

• Bulletin of the Korean Chemical Society
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• v.27 no.9
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• pp.1445-1449
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• 2006

Molecular tweezers based on chenodeoxycholic acid bearing different lengths of arm were synthesized andtheir anion binding affinities were evaluated by $^1H$ NMR, isothermal calorimetric titration, and ESI mass spectrometry. Molecular tweezer 6 showed a high selectivity toward $H_2PO_4\;^-$ over $Cl^-,\;Br^-,\;I^-, $ and $CH_3CO_2\;^-$ by $^1H$ NMR titration, whereas the association constant for $F^-$ revealed the largest value as determined by ITC. The selectivity of 6 towards $F^-$ was about 103 times higher than that of $Cl^-,\;H_2PO_4\;^- $, and $CH_3CO_2\;^-$. ITCexperiment of 6 with $F^-$ in a DMSO showed two binding modes; two sequential association constants $K_1\;=\;2.77\;{\times}\;10^5\;M^{-1}$ and $K_2\;=\;8.68\;{\times}\;10^6\;M^{-1}$ were found. These sequential bindings were confirmed by ESI massspectrometry. 1 : 1 and 1 : 2 complexes of 6 and $F^-$ were found at m/z 868.08 and 884.04.

• Journal of the Optical Society of Korea
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• v.11 no.4
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• pp.162-172
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• 2007

Scanning Laser Optical Tweezers(SLOT) is an optical instrument frequently employed on a microscope with laser being delivered through its various ports. In most SLOT systems, a mechanical tilt stage with a mirror on top is used to dynamically move the laser focal point in two-dimensions. The focal point acts as a tweezing spot, trapping nearby microscopic objects. By adding a mechanical translational stage with a lens, SLOT can be expanded to work in three-dimensions. When two mechanical stages operate together, the focal point can address a closed three-dimensional volume that we call a workspace. It would be advantageous to have a large workspace since it means one can trap and work on multiple objects without interruptions, such as translating the microscope stage. However, previous studies have paid less consideration of the volumetric size of the workspace. In this paper, we propose a new method for designing a SLOT such that its workspace is maximized through optimization. The proposed method utilizes a matrix based ray tracing method and genetic algorithm(GA). To demonstrate the performance of the proposed method, experimental results are shown.

• Proceedings of the Optical Society of Korea Conference
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• 2002.07a
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• pp.108-109
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• 2002

Optical tweezers는 광압(radiation pressure)을 사용하여 입자들을 포획하거나 조절할 수 있다는 점에서 마이크로스케일의 유전체구뿐만 아니라 세포에서도 널리 사용되고 있다. 일반적으로 빛이라는 것은 광자들의 집합체로서 광자의 입자성으로 인하여 외부의 물체와 충돌시 운동량을 전달하게 되고 이것을 광압(radiation pressure)이라고 하며 optical tweezers [1]는 이 광압을 이용한 방법중 하나이다. 레이저빔을 입자에 집속 시켜 주게 되면 입자는 광압에 의해서 gradient force와 scattering force의 힘을 받게 된다. (중략)

• Proceedings of the Optical Society of Korea Conference
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• 2003.02a
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• pp.16-17
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• 2003

수 십 ∼ 수 마이크로 크기의 미세 입자에 강하게 집속된 빔을 산란시키게 되면 입자들은 운동량의 변화에 따라 광의 초점부근에서 포획되는 힘을 받게 된다. 이런 힘은 scattering force와 gradient force로 구분할 수 있고, Optical tweezers는 광의 gradient force를 이용하여 미세입자를 포획하고 조작하는 기술이다. 광에 의해 물리적인 접촉 없이 입자를 포획할 수 있다는 사실로부터 optical tweezers는 생물학을 비롯한 많은 분야에서 유용한 도구로 사용되어지고 있다. (중략)

• Journal of the Optical Society of Korea
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• v.20 no.1
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• pp.78-87
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• 2016

Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationary in interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innate morphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laser beam is an alternative modality to overcome contact-induced perturbation but at the same time a steeply focused laser beam might cause photo-damage. In order to address both the photo-damage and substrate adherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beam of ‘arbitrary intensity profile’ generated by a source having cavity imperfections per se. Thus the immobilized RBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robust Michelson interferometer which was designed from a cubic beam splitter having one surface coated with reflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferograms from bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limited to work in reflection configuration only because of the availability of an upright microscope. Due to RBC’s membrane being poorly reflective for visible wavelengths, quantitative information in the signal is weak and therefore, the quality of experimental results is limited in comparison to results obtained in transmission mode by various holographic techniques reported elsewhere.

• Korean Chemical Engineering Research
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• v.51 no.4
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• pp.527-530
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• 2013

I present the behavior of colloidal adsorption to an oil-water interface in the presence of electrolyte in an aqueous subphase. The optical laser tweezers and the piezo controller are used to trap an individual polystyrene microsphere in water and forcibly transfer it to the interface in the vertical direction. Addition of an electrolyte (i.e., NaCl) in the aqueous subphase enables the particle to attach to the interface, whereas the particle escapes from the trap without the adsorption in the absence of the electrolyte. Based on the analytical calculations of the optical trapping force and the electrostatic disjoining pressure between the particle and the oil-water interface, it is found that a critical energy barrier between them should exist. This study will provide a fundamental understanding for applications of colloidal particles as solid surfactants that can stabilize the immiscible fluid-fluid interfaces, such as emulsions (i.e., Pickering emulsions) and foams.

• International Journal of Control, Automation, and Systems
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• v.2 no.2
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• pp.135-143
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• 2004

Recently, high throughput screening for microorganisms with desired characteristics from a large heterogeneous population has become possible. Single cell separation has taken on increasing significance in recent years, and several different methods have been proposed so far. In this paper, we introduce several cell manipulation methods aiming at single cell separation and investigation. At first, methods for the separation of microorganisms are classified. Then, we introduce two different approaches, that is, (1) indirect manipulation using laser trapped microtools and (2) thermal gelation.

• Proceedings of the Optical Society of Korea Conference
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• 2001.02a
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• pp.272-273
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• 2001

We demonstrate the first separation of neutral molecules using optical forces. Unlike laser atomic cooling or optical tweezers, optical separation technique requires the manipulation of only one component of the molecular motion. Thus the mixtures can be separated, in principle, with less complex schemes. When an Intense nonresonant laser beam is focused onto a beam of molecules, the interaction between the laser electric field and the induced dipole moment of a molecule invokes a mechanical force on the molecule proportional to the field gradient and the molecular polarizability ($\alpha$) to mass (m) ratio $\alpha$/m. (omitted)