• Journal of Microbiology
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• v.45 no.3
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• pp.193-198
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• 2007

A marine bacterium was isolated from Mai Po Nature Reserve of Hong Kong and identified as Vibrio cholerae MP-1. It contains a small plasmid designated as pVC of 3.8 kb. Four open reading frames (ORFs) are identified on the plasmid, but none of them shows homology to any known protein. Database search indicated that a 440 bp fragment is 96% identical to a fragment found in a small plasmid of another V. cholerae. Further experiments demonstrated that a 2.3 kb EcoRI fragment containing the complete ORF1, partial ORF4 and their intergenic region could self-replicate. Additional analyses revealed that sequence upstream of ORF1 showed the features characteristic of theta type replicons. Protein encoded by ORF1 has two characteristic motifs existed in most replication initiator proteins (Rep): the leucine zipper (LZ) motif located at the N-terminal region and the alpha helix-turn-alpha helix motif (HTH) located at the C-terminal end. The results suggest that pVC replicates via the theta type mechanism and is likely a novel type of theta replicon.

• Journal of Microbiology and Biotechnology
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• v.7 no.2
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• pp.132-137
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• 1997

A novel protein (HEAAE, High Essential Amino Acid Encoding Protein), rich in essential amino acids ($75{\%}$ of total), was designed and constructed in our laboratory. The designed peptides were analyzed by SYBLE and stable secondary and tertiary structures were predicted. The monomeric form (HEAAE-1) of the protein consists of 20 amino acid residues with four additional amino acids comprising a potential ${\beta}$-turn (HEAAE-4). Size exclusion analysis demonstrated that the monomer is self-aggregates in aqueous solution to form higher ordered multimeric structures, which are very reminiscent of natural plant storage proteins. The DNA encoding this amino acid sequence was synthesized, and from this monomeric gene fragment (heaae-1), the stable tetrameric form of the gene (heaae-4) was generated by subcloning into the E. coli expression vector pKK223-3. A clear 6 kDa polypeptide band corresponding to the molecular weight of the dimeric form (HEAAE-2) was detected. The smeared band which appeared around the molecular weight corresponding to HEAAE-4 of 11 kDa suggested that the tetramer form of this protein might be processed into smaller size products.

• Journal of Microbiology and Biotechnology
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• v.8 no.3
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• pp.280-283
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• 1998

We have cloned the RNA polymerase sigma-like factors from a wide range of actinomycetes by using specific primers with the polymerase chain reaction (PCR). The specific oligonucleotide primers were designed on the basis of amino acid sequences of conserved regions from HrdA, B, D of Streptomyces griseus as well as from the rpoD box of many eubacteria. The consensus sequences were from the rpoD box and helix-turn-helix motif involved in -35 recognition. The designed primers were successfully applied to amplify the DNA fragments of the hrd homolog genes from 8 different strains of actinomycetes which produce a wide variety of important antibiotics. The 480 bp of the DNA fragment was amplified from all 8 strains, and it was identified as a part of hrdA and hrdB as we designed. The deduced amino acid sequence of PCR-amplified DNA fragments were highly homologous to those of other known RNA polymerase sigma factors of S. griseus and Streptomyces aureofaciens. Therefore, this study with specifically designed primers will support rapid cloning of the RNA polymerase sigma factors which recognize different classes of promoters from actinomycetes, and it will also be helpful in understanding the relationship of promoters and sigma factors leading to heterogeneity of RNA polymerases in actinomycetes.

• Journal of the Korean Magnetic Resonance Society
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• v.10 no.2
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• pp.163-174
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• 2006

Luteinizing Hormone Releasing Hormone(LHRH) is a decapeptide neurotransmitter known to be regulated by metal ions in the hyperthalamus. Zn-binding LHRH complex was systhesized, and zinc-LHRH complex was studied to understand what kinds of structural modifications would be critical in the LHRH releasing mechanism. Both nonexchangeable and exchangeable $^1H-NMR$ signal assignments were accomplished by pH-dependent and COSY NMR experiments. In addition, $^1H-NMR$ chemical shift changes of a-proton and peptide NH NMR signals at different pH condition, and $^1H-NMR$ signal differences between metal free and metallo-LHRH complex was monitored. NMR signals exhibit that primary metal-binding sites are nitrogens donor of imidazole ring and Arg, and peptide oxygen of Pro-His in the sequence. Structure obtained in this study has a cyclic conformation which is similar to that of energy minimized, and exhibits a specific a-helical turn with residue numbers $(2{\sim}7)$ out of 10 amino acids.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.5 no.2
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• pp.287-312
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• 2011

Internet chat programs and instant messaging services are becoming increasingly popular among Internet users. One of the crucial issues with Internet chat is how to manage the corresponding pairs of questions and answers in a sequence of conversations. Although many novel methodologies have been introduced to cope with this problem, most are poor in managing interruptions, organizing turn-taking, and conveying comprehension. The Internet environment is recently evolving into a 3D environment, but the problems with managing chat dialogues with the standard 2D text-based chat have remained. Therefore, we propose a more realistic communication model for chat agents in 3D virtual space in this paper. First, we propose a new method to measure the capacity of communication between chat agents and a novel visualization method to depict the hierarchical structure of chat dialogues. In addition, we are concerned with communication networks for virtual people (avatars) living in virtual worlds. In this paper we consider a microscopic aspect of a social network in a relatively short period of time. Our experiments show that our model is highly effective in a virtual chat environment, and the communication network based on our model greatly facilitates investigation of a very large and complicated communication network.

• Journal of Power Electronics
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• v.19 no.4
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• pp.968-979
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• 2019

Recently, there has been increased interest in the study of multiphase machines due to their higher fault-tolerant capability when compared to their conventional three-phase counterparts. For six-phase machines, stator windings configured with a single isolated neutral (1N) provide significantly more post-fault torque/power than two isolated neutrals (2N). Hence, this configuration is preferred in applications where post-fault performance is critical. It is well known that min-max injection has been commonly used for three-phase and multiphase machines in healthy condition to maximize the modulation limit. However, there is a lack of discussion on min-max injection for post-fault condition. Furthermore, the effects in terms of the common-mode voltage (CMV) in modulating signals has not been discussed. This paper investigates the effect of min-max injection in post fault-tolerant control on the voltage and speed limit of a symmetrical six-phase induction machine with single isolated neutral. It is shown that the min-max injection can minimize the amplitude of reference voltage, which maximizes the modulation index and post-fault speed of the machine. This in turn results in a higher post-fault power.

• The Transactions of the Korean Institute of Power Electronics
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• v.27 no.2
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• pp.165-173
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• 2022

The electrification trend of mobility increases every year due to the development of power semiconductor and battery technology. Accordingly, the development and distribution of fast chargers for electric vehicles (EVs) are in demand. In this study, we propose a design and implementation method of an LLC converter for fast chargers. Two 15 kW LLC converters are configured in parallel to have 30 kW rated output power, and the control algorithm and driving sequence are designed accordingly and verified. In addition, the improved power conversion efficiency is confirmed through zero-voltage switching (ZVS) of the LLC converter and reduction of turn-off loss through snubber capacitors. The implemented 30 kW LLC converters show a wide output voltage range of 200-950 V. Experiments applying various load conditions verify the converter performance.

• Journal of Intelligence and Information Systems
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• v.14 no.4
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• pp.103-116
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• 2008

This paper proposes quay crane (QC) scheduling algorithms that determine the working sequence of QCs over ship bays in a container vessel in automated container terminals. We propose two scheduling algorithms that examine the distribution of export containers in the stacking yard and determine the sequence of ship bays to balance the workload distribution among the yard blocks. One of the algorithms is a simple heuristic algorithm which dynamically selects the next ship bay based on the entropy of workloads among yard blocks whenever a QC finishes loading containers at a ship bay and the other uses genetic algorithm to search the optimal sequence of ship bays. To evaluate the fitness of each chromosome in the genetic algorithm, we have devised a method that is able to calculate an approximation of loading time of container vessels considering the workloads among yard blocks. Simulation experiments have been carried out to compare the efficiency of the proposed algorithms. The results show that our QC scheduling algorithms are efficient in reducing the turn-around time of container vessels.

• Korean Institute of Interior Design Journal
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• v.23 no.1
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• pp.61-68
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• 2014

Recently, there have been increasing attempts to pursue and express feelings such as sensibilities, emotions, and impressiveness in commercial spaces. One of such methods is to apply 'storytelling' to space designs. Applicability of storytelling to a space suggests that the contents of a space can be expressed through various mediums. Portraying events and situations through a single time continuity of a story is referred to as 'narrative'. The movement of users and sequence of contents are determined by a narrative. It provides different storytelling and a sense of place to each space through various roles, such as wide association, engraving, and image formation. A narrative can lead users to engage in different perceptions and behaviors even in spaces with the same content. Thus, this study is intended to examine the impact of space marketing in line with design narratives, assuming that narratives of commercial space designs will influence the formation of brand identity. The research methods are as follows. First, the definition of narratives in space design was established by examining narrative architectures. Second, design analysis tools for commercial spaces were established from the perspective of narratives through preceding studies. Third, the design narratives of different shops under the same brand were comparatively analyzed through a case study. To carry out a case study, a commercial space of 'O'sulloc' was selected, and its brand identity was studied from the narrative standpoint. The case study involved interior designs of 7 road shops of 'O'sulloc.' Among the 7 road shops, two of them with the biggest difference in design narratives were selected, and an observation survey was done on the users as a second analysis. Through the observation survey, actual design narrative experience was analyzed in 4 steps of introduction, development, turn, and conclusion. The findings are as follows. The design method of each shop varied, and different design elements were emphasized. Among various elements, the ones that reflect the brand identity of 'O'sulloc' the best were logo, product, and shape. During the process of narratives, the characteristics of each shop and user recognition and behavior varied depending on the degree of emphasis on a particular element. It suggests that space design narratives can influence the formation of brand identity. This study provides ideal directions of developing space designs necessary for forming brand identity from the standpoint of Korean traditional culture modernization. Future studies could discuss the economic feasibility of such designs.

• Reproductive and Developmental Biology
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• v.30 no.3
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• pp.157-162
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• 2006

Endogenous 84 amino acid parathyroid hormone(PTH) is synthesized as a pre-pro hormone by the chief cells of the parathyroid glands. Physiological actions of PTH include regulation of bone metabolism, renal tubular reabsorption of calcium and phosphate, and intestinal calcium absorption. In addition, PTH stimulates new bone formation by extraordinary stimulation of osteoblastic activity and decreasing calcium excretion by the kidney. In this study, we constructed and tested retrovirus vectors designed to express the human parathyroid hormone(hPTH) gene under the control of the tetracycline-inducible promoters. To increase the hPTH gene expression at turn-on state, woodchuck hepatitis virus posttranscriptional regulatory element(WPRE) sequence was also introduced into retrovirus vector at downstream region of either the hPTH gene or the sequence encoding reverse tetracycline-controlled transactivator(rtTA). Transformed primary culture cells(porcine fetal fibroblast, PFF, chicken embryonic fibroblast, CEF) were cultured in the medium supplemented with or without doxycycline(tetracycline derivative) for 48 hours, and induction efficiency was measured by comparing the hPTH gene expression level using two step RT-PCR and ELISA Higher hPTH expression($3{\tims}10^4\;pg/ml,\;5.3{\times}10^4\;pg/ml$) and tighter expression control(up to 8 fold) were observed from the vector in which the WPRE sequence was placed at downstream of the hPTH gene. The resulting tetracycline inducible vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals.