Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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pVC, a Small Cryptic Plasmid from the Environmental Isolate of Vibrio cholerae MP-1

  • Zhang, Ruifu (Laboratory of Environmental Microbiology and Toxicology, Department of Ecology & Biodiversity, The University of Hong Kong) ;
  • Wang, Yanling (Laboratory of Environmental Microbiology and Toxicology, Department of Ecology & Biodiversity, The University of Hong Kong) ;
  • Leung, Pak Chow (Biomolecular Interaction Laboratory, Department of Zoology, The University of Hong Kong) ;
  • Gu, Ji-Dong (Laboratory of Environmental Microbiology and Toxicology, Department of Ecology & Biodiversity, The University of Hong Kong)
  • Published : 2007.06.30
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Abstract

A marine bacterium was isolated from Mai Po Nature Reserve of Hong Kong and identified as Vibrio cholerae MP-1. It contains a small plasmid designated as pVC of 3.8 kb. Four open reading frames (ORFs) are identified on the plasmid, but none of them shows homology to any known protein. Database search indicated that a 440 bp fragment is 96% identical to a fragment found in a small plasmid of another V. cholerae. Further experiments demonstrated that a 2.3 kb EcoRI fragment containing the complete ORF1, partial ORF4 and their intergenic region could self-replicate. Additional analyses revealed that sequence upstream of ORF1 showed the features characteristic of theta type replicons. Protein encoded by ORF1 has two characteristic motifs existed in most replication initiator proteins (Rep): the leucine zipper (LZ) motif located at the N-terminal region and the alpha helix-turn-alpha helix motif (HTH) located at the C-terminal end. The results suggest that pVC replicates via the theta type mechanism and is likely a novel type of theta replicon.

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References

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