• Journal of the Korean Magnetic Resonance Society
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• v.8 no.1
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• pp.19-27
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• 2004

C-terminal fragments of APP (APP-CTs), that contain complete Abeta sequence, are found in neuritic plaques, neurofibrillary tangles and the cytosol of lymphoblastoid cells obtained from AD patients. CT16, Lys649-Asp664 (KKQYTSIHHGVVEVD) has been known as the most toxic part in the C-terminal fragment of amyloid precursor protein (APP). The solution structure of CT16 was investigated using NMR spectroscopy in various membrane-mimicking environments. According to Circular Dichroim (CD) spectra, CT16 has a random structure in aqueous solution, while conformational change was induced by addition of TFE and SDS micelle. Tertiary structure as determined by NMR spectroscopy shows that CT16 has a ${\beta}$-turn conformation in trifluoroethanol-containing aqueous solution.

• Journal of Hydrospace Technology
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• v.1 no.1
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• pp.1-13
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• 1995

Vortex shedding from a circular cylinder is simulated by means of the discrete vortex method. The shear layer emanating from the separation point is approximated as a sheet vortex which is in turn represented by a sequence of discrete vortices. The strength of these vortices is calculated from the vorticity shedding rate and introduced at a small distance off the side ($\Theta=\pm\pi/2$) of the cylinder surface in regular time step. Sheet vortex cutting, rediscretization and replacement of vortex by vortex segment are put to use to enhance stability of the sheet vortex evolution. The simulated vortex distribution pattern very well reproduces structure like the Karman vortex street. However, as for the force coefficients, the qualitative properties are correctly predicted but some more improvements are needed for the quantitative accuracy.

• Journal of the Korean earth science society
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• v.40 no.2
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• pp.163-176
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• 2019

The Potosinian geological basement in central Mexico is comprised of the Upper Paleozoic metamorphic rocks, which crop out on the Sierra de Catorce nucleus located in the northeastern part of the state. The sedimentary sequence that covers unconformably the Paelozoic basement is represented by an Upper Triassic marine sedimentary sequence, correlating to the Zacatecas Formation and the Upper Triassic continental Huizachal Formation red beds, which in turn are covered either by La Joja Formation Jurassic red beds or by Upper Jurassic marine sediments. This sequence is overlain by the conformable Cretaceous calcareous marine sedimentary rocks in all the state of San Luis Potosi. The Cenozoic sequence unconformably covers some of the aforementioned rocks and is represented by undifferentiated volcanic rocks as well as by marine clastic rocks. The existing intrusive igneous rocks are felsic to intermediate composition, and they intrude the metamorphic basement and sedimentary rocks. Conglomerates with evaporitic sediments were deposited during the Pleistocene. The Quaternary sequence includes basalt flows, piedmont deposits, alluvium, and occasionally evaporites and caliche layers. In the state of San Luis Potosi, a great diversity of mineral deposit types is known as both metallic and nonmetallic. The host rocks of these deposits vary from one another including formations that represent from Paleozoic up to Tertiary. The mineralization age corresponds approximately to Tertiary (75%), and is mainly epigenetic. Conclusively, the data on geology and mineralization in San Luis Potosi, Mexico are helpful to predict a hidden ore body and select promising mineralized zone(s) when the domestic company makes inroads in the mining sector of Mexico.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.31 no.2
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• pp.190-198
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• 1995

Many industrial servo amplifiers employ power transister as output device. Thyristor converters are not adopted to drive servo motor, although thyristor is superior to power TR in power rating, noise immunity, price, and size. The reason is, thyristor has no ability of self turn - off. Here in this paper line commutation, in which thyristor is turned off naturally since cathode voltage is higher than anode as time goes by, is employed to turn on thyristor with a delicate sequence. We developed thyristor servo amplifier which does not cause any damage on thyristor because it is designed to prevent triggering the two SCRs in the same arm simultaneously. And it was made clearly how to trigger SCR without any power line shorting and also harmonic analysis is carried out with the aid of FFT analyzer and proved that it can be used even severe reactive load. The designed circuit operated as a good DC amplifier in conventinal servomotor and the results can be use as a position control system application.

• Journal of Korean Society of Transportation
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• v.22 no.2 s.73
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• pp.131-143
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• 2004

Given a path represented by a sequence of link numbers in a graph, the vine is differentiated from the loop in a sense that any link number can be visited in the path no more than once, while more than once in the loop. The vine provides a proper idea on complicated travel patterns such as U-turn and P-turn witnessed near intersections in urban transportation networks. Application of the link label method(LLM) to the shortest Path algorithms(SPA) enables to take into account these vine travel features. This study aims at expanding the LLM to a K-th path search algorithm (KPSA), which adopts the node-based-label correcting method to find a group of K number of paths. The paths including the vine type of travels are conceptualized as drivers reasonable route choice behaviors(RRCB) based on non-repetition of the same link in the paths, and the link-label-based MPSA is proposed on the basis of the RRCB. The small-scaled network test shows that the algorithm sequence works correctly producing multiple paths satisfying the RRCB. The large-scaled network study detects the solution degeneration (SD) problem in case the number of paths (K) is not sufficient enough, and the (K-1) dimension algorithm is developed to prevent the SD from the 1st path of each link, so that it may be applied as reasonable alternative route information tool, an important requirement of which is if it can generate small number of distinct alternative paths.

• Cartoon and Animation Studies
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• s.39
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• pp.183-214
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• 2015

For a long time, Disney has made animations including world views such as family-focused, conservative ideology, happy ending, etc. for families. Periodical situation and spectators' demands have been changed little by little, but Disney is still a company who is the most successful both nominally and virtually in animation industry. For the secrets of their success, there is fantasy world of fabricated animation, thoroughly organized story and image. When spectators watch animations, their sufficient immersion in the fantasy is to let them forget realities. In the introduction of Disney animations, spectators turn off the switch of realities and turn on the switch of fantasy world through the procedure to recognize and confirm virtual world sufficiently. In the switch of the introduction used frequently by Disney, there are four methods. First is to start while opening the cover of storybook. Second is to start while storyteller gives a talk directly. Third is to start with live action film and continue to animation. Fourth is musical method. Those four switches play a role of the gate which distinguishes spectators' realities from Disney's fantasy clearly. The process makes an opportunity to set spectators who feel uneasy due to the vague boundary between realities and fantasy at ease and let them immerse in animation sufficiently with mind at ease.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.1
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• pp.116-121
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• 2010

Long-chain polyunsaturated fatty acids (LC-PUFA) promote the development of brain and vision of the fetus, relieve inflammation, inhibit oral dysplasia of rumor cell, decrease the incidence of cardiovascular disease and regulate arrhythmia. ${\Delta}-6$ desaturase is the rate-limited enzyme in the desaturation process. This study reports the cloning, characterization and tissue expression of a ${\Delta}-6$ desaturase gene in the chicken. PCR primers were designed based on the predicted sequence of chicken ${\Delta}-6$ desaturase (accession number: XM421053) and used to isolate a cDNA fragment of 1,323 bp from chicken liver. Based on the 1,323 bp fragment an EST (BI390105) was obtained by BLAST. The EST and 5'nd of the 1,323 bp fragment were partially overlapped. Gene specific primers derived from the EST were used for amplification of the 5'nd. Another gene-specific primer derived from the 1,323 bp fragment was used for amplification of the 3'nd by 3'ACE. Then the three overlapping cDNA sequences obtained were assembled with DNAMAN software and a full-length ${\Delta}-6$ desaturase of 2,153 bp was obtained. The full-length cDNA contained an ORF of 1,335 bp with a 5'ntranslated region of 147 nucleotides followed by an ATG initiation codon. Stop codon TGA was at position 1,481-1,483 bp. The deduced amino acids shared an homology above 77% with bovine, mice, orangutan, rat and human. The protein sequence had three histidine-rich regions HDFGH (HisI region), HFQHH (HisII region) and HH (HisIII region), a cytochrome $b_{5}$-like domain containing a heme-binding motif and two transmembrane domains. Sequence analysis of the chicken genomic DNA revealed that the coding sequence of chicken ${\Delta}-6$ desaturase included 12 exons and 11 introns. Semi-quantitative RT-PCR showed that the ${\Delta}-6$ desaturase expression levels were in turn liver, spleen, pancreas, lung, breast muscle, heart, and abdominal fat. The expression of ${\Delta}-6$ desaturase in liver was significantly higher than that in breast muscle (p<0.01). The expression of ${\Delta}-6$ desaturase in lung was significantly higher than that in abdominal fat (p<0.01). This is the first clone of chicken ${\Delta}-6$ desaturase.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.2
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• pp.44.1-44.1
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• 2016

The Sagittarius-Carina spiral arm in the Galaxy contains several massive young open clusters. We present a deep optical photometric study on the massive young open clusters in the Sagittarius-Carina arm, Westerlund 2 and the young open clusters in the ${\eta}$ Carina nebula. Westerlund 2 is a less studied starburst-type cluster in the Galaxy. An abnormal reddening law for the intracluster medium of the young starburst-type cluster Westerlund 2 is determined to be $R_{V,cl}=4.14{\pm}0.08$. The distance modulus is determined from zero-age main-sequence fitting to the reddening-corrected color-magnitude diagrams of the early-type members to be $V_0-M_V=13.9{\pm}0.14mag$. The pre-main sequence (PMS) members of Westerlund 2 are selected by identifying the optical counterparts of X-ray emission sources from the Chandra X-ray observation and mid-infrared emission sources from the Spitzer/IRAC (the Infrared Array Camera) observation. The initial mass function (IMF) shows a slightly flat slope of ${\Gamma}=-1.1{\pm}0.1$ down to $5M_{\odot}$. The age of Westerlund 2 is estimated to be. 1.5 Myr from the main-sequence turn-on luminosity and the age distribution of PMS stars. The ${\eta}$ Carina nebula is the best laboratory for the investigation of the Galactic massive stars and low-mass star formation under the influence of numerous massive stars. We have performed deep wide-field CCD photometry of stars in the ${\eta}$ Carina nebula to determine the reddening law, distance, and the IMF of the clusters in the nebula. We present VRI and $H{\alpha}$ photometry of 130,571 stars from the images obtained with the 4m telescope at Cerro Tololo Inter-American Observatory (CTIO). RV,cl in the η Carina nebula gradually decreases from the southern part (~4.5, around Trumpler 14 and Trumpler 16) to the northern part around Trumpler 15 (~3.5). Distance to the young open clusters in the ${\eta}$ Carina nebula is partly revised based on the zero-age main-sequence fitting to the reddening-corrected color-magnitude diagrams (CMDs) and the (semi-) reddening-independent CMDs. We select the PMS members and candidates by identifying the optical counterparts of X-ray sources from the Chandra Carina Complex Survey and mid-infrared excess emission stars from the Spitzer Vela-Carina survey. From the evolutionary stage of massive stars and PMS stars, we obtain that the northern young open cluster Trumpler 15 is distinctively older than the southern young open clusters, Trumpler 14 (${\leq}2.5 Myr$) and Trumpler 16 (2.5-3.5 Myr). The slopes of the IMF of Trumpler 14, Trumpler 15, and Trumpler 16 are determined to be $-1.2{\pm}0.1$, $-1.5{\pm}0.3$, and $-1.1{\pm}0.1$, respectively. Based on the RV,cl of several young open clusters determined in this work and the previous studies of our group, We suggest that higher RV,cl values are commonly found for very young open clusters with the age of < 4 Myr. We also confirm the correlation between the slope of the IMF and the surface mass density of massive stars.

• Reproductive and Developmental Biology
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• v.29 no.1
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• pp.49-55
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• 2005

We cloned cDNA of the PGH(porcine growth hormone) gene and constructed retrovirus vector designed to express PGH gene under the regulation of CMV (cytomegalovirus) promoter. To maximize the expression, WPRE(woodchuck hepatitis virus posttranscriptional regulatory element) sequence was placed at the downstream of the PGH gene. After infection with recombinant viruses, approximately 1×10/sup 6/ PFF(porcine fetal fibroblast) cells released PGH protein into the media as much as 1,400 ng. In a subsequent experiment, a modifications of the retrovirus vector was made to express the PGH gene in a teracycline-inducible manner. In PFF cells carrying these viral vector sequences, addition of doxycycline to the media resulted in 2∼6 fold increase in PGH synthesis. In the modified retrovirus vectors, the WPRE sequence also played a role in boosting the effect of the tetracycline induction. This result indicates that our tetracycline-inducible expression system might be a promising candidate in alleviating the complicate physiological problems caused by constitutive expression of the exogenous genes in the transgenic animals.

• Journal of the Korea Society of Computer and Information
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• v.16 no.9
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• pp.27-33
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• 2011

In this paper, we propose an efficient on-line handwritten digit recognition base on Convex-Concave curves feature which is extracted by a chain code sequence using Smith-Waterman alignment algorithm. The time sequential signal from mouse movement on the writing pad is described as a sequence of consecutive points on the x-y plane. So, we can create data-set which are successive and time-sequential pixel position data by preprocessing. Data preprocessed is used for Convex-Concave curves feature extraction. This feature is scale-, translation-, and rotation-invariant. The extracted specific feature is fed to a Smith-Waterman alignment algorithm, which in turn classifies it as one of the nine digits. In comparison with backpropagation neural network, Smith-Waterman alignment has the more outstanding performance.