• Korean Journal of Weed Science
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• v.8 no.2
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• pp.187-198
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• 1988

The second leaves from 30 days old seedlings of two rice cultivars which were selected as tolerant (cv. Chokoto) and susceptible (cv. Weld pally) cultivar were soaked in the concentration of $10^{-6}$, $10^{-5}$, $10^{-4}$ and $10^{-3}M$ of oxyfluorfen for 10, 15 and 22hrs and anatomical characteristics were abserved. Dipping to the solutions were carried out either directly to the attached leaves or to the seperated leaves. Development of any symptoms in epidermis, bundle sheath, mesophyll cells and bulliform cells were microscopically inspected. Both cultivars showed reductionin leaf thickness, but the susceptible ones was more sensitive than the tolerant. The degradation and disappearance of epidermal cell layer, breakage of bundle sheath cells, shrinkage of mesophyll cells and disappearance of bulliform cells were general response as affected by oxyfluorfen treatment. The susceptible cultivars showed such responses at the concentration of $10^{-5}M$ for 10 hrs while tolerant ones $10^{-3}M$, for 10 hrs. Those treatments were more effective in seperated leaves than in attached ones. The epicuticular wax layer of leaves treated as above for 20 hrs was inspected by SEM. Weld pally, the susceptible cultivar (Weld pally) showed rapid cleavage of wax layer under $10^{-5}M$ concentration while the tolerant (Chokoto) showed only minor damage on wax layer at the concentration of $10^{-3}M$.

• Korean Journal of Plant Tissue Culture
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• v.28 no.5
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• pp.277-281
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• 2001

Embryogenic callus induction and plant regeneration methods were developed for native Kentucky bluegrass (Poa pratenes L.) ecotypes. Mature caryopses and immature inflorescences (20 mm in length) of 4 native ecotypes and 5 foreign cultivars were plated on MS medium (30 g/L sucrose, 3 g/L Phytagel) supplemented with 1 mg/L 2,4-D, and cultured in the dark at 24$^{\circ}C$. Most explants formed calli, but more embryogenic calli were induced from the explants of immature inflorescences than caryopses which produced mostly non-embryogenic rooty calli. In P77 ecotypes, immature inflorescence explants formed embryogenic calli with the rate of 62~95%, and those of field-grown plants were more efficient than greenhouse-grown ones in embryogenic callus induction. Plantlets were regenerated from the embryogenic calli when they were transferred to hormone-free MS medium, and grew to maturity without morphological variations in greenhouse.

• Korean Journal of Weed Science
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• v.31 no.3
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• pp.308-312
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• 2011

A new paddy field herbicide, metazosulfuron granule is an herbicide for controlling annual and perennial weeds occurring in machinery transplanting paddy field. It is recommended to apply $30kg\;ha^{-1}$ 15 days after transplanting. As a result of preliminary tests, it could control seven annual species such as Echinochloa crus-galli, Bidens tripartita, Ludwigia prostrata, Monochoria vaginalis var. plantaginea, etc. and four perennial species including Cyperus serotinus, Sagittaria trifolia, Eleocharis kuroguwai, and Scirpus juncoides. Monochoria vaginalis var. plantaginea, S. trifolia and S. juncoides resistant to sulfonylurea- herbicides were also controlled. Because application timing of the herbicide is wide, it is very efficient to control the spraying timing by users. Phytotoxicity of rice cultivars was not observed, and the impact on the environment is very limited.

• Korean Journal of Weed Science
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• v.9 no.2
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• pp.103-107
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• 1989

Five rice cultivars were treated by four dose of butachlor at transplanting seedling stage. Visual injury rate by butachlor was lowest in Zhy-Lian-Ai-dun-Nam and highest in Samseungbyeo, but reduction of yield was higher in Zhy-Lian-Ai-Yun-Nam and Hangangchalbyeo than in Samseungbyeo and Cheungcheungbyeo. Reduction of panicle number by butachlor was most effective factor to yield reduction.

• Horticultural Science & Technology
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• v.34 no.2
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• pp.342-353
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• 2016

This study was initiated to evaluate green color retention under three different soil systems. Several turfgrasses were evaluated in multi-layer, USGA, and mono-layer systems. Turfgrass entries were comprised of three cultivars of Korean lawngrass (Zoysia japonica Steud.) as warm-season grass (WSG) and three blends and three mixtures of Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), and tall fescue (TF, Festuca arundinacea Schreb.) as cool-season grass (CSG). Significant differences were observed in visual turf color and green color retention among soil systems and turfgrasses. Both the multi-layer and USGA systems were highly associated with better color ratings and longer color retention, as compared with the mono-layer system. Seasonal variation of visual turf color greatly occurred from late December to early spring. CSG exhibited longer color retention than did WSG. The latter maintained green color for approximately 6 months, regardless of the soil system. Spring green-up of Korean lawngrass occurred from early to middle May, while it underwent discoloration from late October to early November. Among the CSGs green-up occurred between early March and early April and leaf color was maintained until middle December to early February. Therefore, the CSGs were green for 8.5 to 11 months, depending on turfgrass and soil system. The mean period of green color duration across all soil systems was approximately 10-11, 9-10 and 8.5-9.0 months for PR, KB and TF, respectively. As for the CSG mixtures, the greater the proportion of PR, the longer the green color retention, while the higher the proportion of TF, the shorter the color retention. There was greater variation in green color duration among the CSGs than the WSGs. Across soil systems, color retention differences of 2 to 6 days were observed for the Korean lawngrass, but 7 to 36 days for the CSGs. These results demonstrate that green color retention varied greatly according to soil systems and also among turfgrasses. Selections of turfgrass and soil system should be made using a concept-oriented approach, when establishing garden, park, soccer field, golf course and other sports field. Information obtained in this study can be used to select soil systems and turfgrasses based on the expected degree of leaf color retention.

• Korean Journal of Weed Science
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• v.17 no.1
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• pp.31-43
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• 1997

This study was carried out to investigate classfication of weedy rice (Oryza sativa) based on isozymes esterase and peroxidase, growth and developmental difference of weedy rices and rices grown under dry and water condition, and weedy rice control and tolerant difference of weedy rices in various herbicides using weedy rices collected from thirteen strains of Chonnam, one Chonbuk, two Kyeongki and two rice cultivars. 1. The collected weedy rices were classified into three groups based on isozyme esterase and peroxidase using polyacrylamide gel electrophoresis(PAGE) method. The classified groups were not same each other. 2. Plant height was taller in collected weedy rices than rice cultivars at 18 days after seeding under dry and water conditions, but number of leaves, shoot fresh weight, root fresh weight and root length were not significantly different between collected weedy rices and rice cultivars. In addition, growths of collected weedy rices were greater in dry- than water-condition. 3. After thiobencarb(S-4-chlorobenzyl diethythiocarbamate), molinate(S-ethyl hexahydro-1H-azepine-1-carbothioate) and oxadiazon(5-tert-butyl-3(2,4-dichloro-5-isopropoxyphenyl)-1,3,4-oxadiazol-2-one) were applied at 6 days before seeding, the weedy rices controlled 100% by thiobencarb at 2.1kg ai/ha and 024kg ai/ha oxadiazon treatment but controlled 26% to 67% by molinate at 6.5kg ai/ha. Rice due to the herbicides was injured severely(25% to 100%) in flood condition at time of rice seeding after oxadiazon at 0.48kg ai/ha and 2.1kg ai/ha thiobencarb application, except for molinate which injured rice slightly(4% to 13%) in drain condition. The collected weedy rices to all experimented herbicides showed slight intraspecific variations. The intraspecific variations of weedy rices decreased in the order of thiobencarb>molinate>oxadiazon.

• Korean Journal of Weed Science
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• v.11 no.1
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• pp.11-18
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• 1991

Seven formulations of oxyfluorfen selected from the previous studies(4. 5) were tesed to evaluate crop injury and herbicidal efficacy on two rice cultivars and several annual and perennial weeds in a greenhouse. Each formulation at two different rates was applied to rice transplanted with 8-, 22- and 32-day old seedlings and to direct-seeded rice. Among the formulations, Elvan, Bentonite B. Chitosan and Coal Slag gave lower injury than a control formulation, Sand-coated oxyluorfen, and they did not have a problem with excessive release if active ingredient at once. Especially, the formulations of Elvan, Chitosan and Bentonite B controlled annual weeds (Echinochloa crus-galli, Monochoria vaginalis, Cyperus difformis., and Scirpus juncoides) and perennial weeds (Sagittaria pygmaea, and Cyperus serotinus). The surface structure of the formulations indicate the different possibilities of releasing of oxyfluorfen by different cracking and hole sizes, namely retention capacity.

• Korean Journal of Weed Science
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• v.10 no.4
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• pp.285-293
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• 1990

The response of callus growth of rice (Oryza sativa L.) cultivars which showed different responses to herbicides as seedlings was investigated to select resistant or tolerant calli. Callus growth of IR28 which was susceptible to thiobencarb (S-[(4-chlorophenyl)methyl]diethylcarbamothioate) during callus induction was not inhibited by $10^{-5}$ M and $10^{-6}$ M thiobencarb, indicating that there was a difference in tolerance among callus induction and growth, and the intact plant level. A similar result was obtained with IR31917-45-3-2-2 to butachlor [N - (buthoxymethyl) -2-chloro-N- (2, 6-diethylphenyl) acetamide]. The fresh weight of IR28 callus transferred into $10^{-5}$ M thiobencarb after treatment at $10^{-6}$ M for 30 days was not affected by the herbicide, indicating that transferring callus into gradually higher herbicide concentrations can be a useful method for selection of herbicide-tolerant cell lines.

• Korean Journal of Weed Science
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• v.8 no.3
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• pp.244-249
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• 1988

Some experiments were conducted to utilize the raphanus test as a bioassay method for plant growth regulaters and hormonal herbicides. Among seven radish cultivars, the response of Jangbaegminong was the most sensitive and quantitative to ${\alpha}$-NAA and its coefficient of variation value was the lowest. The optimum application time of test compounds was estimated seven to nine days after seeding. After ${\alpha}$-NAA treatment the curvature angle of radish cotyledonary petiols was sharply increased to the maximum value at 24 hours after and was changed by light and temperature conditions. The curvature response was observed by application of ${\alpha}$-NAA, phenoxy and indole compounds but often plant growth regulators, $GA_3$ benzyladenine, ABA and homobrassinolide was not detected by this method. So this bioassay was useful to evaluate the activity of a compound as an auxin or a hormonal herbicide.

• Korean Journal of Weed Science
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• v.6 no.2
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• pp.191-200
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• 1986

The study was conducted to screen paraquat-tolerant plant species among crops and weeds, using the response of plant like leaf disc discoloration, visual injury and dry weight in the presence of paraquat. Mechanism of paraquat-tolerance was investigated in strains of soybean through evaluating activities of superoxide dismutase and peroxidase and the multiplication of callus derived from soybean cotyledon. In crops, Kwanggyo has been selected as a paraquat-tolerant variety among soybean cultivars tested, and Hood as a susceptible one. In weeds, Polygonum aviculare, Chenopodium album and Pinellia ternata were evaluated as the paraquat resistant species, providing the possibility for the donor plant species for paraquat resistance. Activity of superoxide dismutase known to detoxify paraquat was markedly greater in Kwanggyo, a paraquat-tolerant cultivar than in Hood, a susceptible one. In addition, the similar response like superoxide dismutase was observed in peroxidese activity. The greater inhibition of callus multiplication was determined in Hood, a susceptible one than a tolerant one, Kwnggyo. Based on all the informations, it is strongly proposed that paraquat tolerance in soybean is due to destruction of $O_2^-$ by elevated concentration of superoxide dismutase in the tolerant cultivar.