• Weed & Turfgrass Science
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• v.6 no.1
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• pp.67-76
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• 2017

The study was initiated to evaluate the effects of overseeding warm-season grass (Zoysia japonica Steud.) with cool-season grasses (CSG) on turfgrass density, uniformity and tiller appearance and to determine turfgrass species and seeding rate applicable for a practical use. Treatments were comprised of Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), tall fescue (TF, Festuca arundinacea Schreb.) and their mixtures. Overall turfgrass density and uniformity were much better with the overseeded treatments over the control. In early stage after overseeding, the greater the PR in treatments, the greater the turfgrass density and uniformity. But the higher the KB, the lower the density and uniformity. From the middle-stage evaluation, however, we observed the opposite results as compared with early-stage findings. Accordingly, the KB was highest in turfgrass density and uniformity, while the PR lowest. In regards of mixtures, both turfgrass density and uniformity were better with increased KB and decreased PR in overseeding rates. As for a medium-quality mixtures of Korean lawngrass with CSG, it would be the best choice to apply with KB at $50g\;m^{-2}$ and equal combination of KB, PR and TF by 1/3 in mixing at $75g\;m^{-2}$ in terms of sustainable density and uniformity.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.191-197
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• 2013

Two medium-leaf ecotypes (CY6069, CY6097) belonging to one species (Zoysia japonica) of Korean lawngrasses were selected in sod production fields in Jang Seong, Korea. They were reported to have distinct morphological and growth rate characteristics different from the preferred medium-leaf type zoysiagrass in Korea. This study was conducted to define further the genotypic difference at the molecular level and to develop DNA marker based on the specific DNA fragment. Polymorphic DNA fragments were first explored by using randomly amplified polymorphic DNA (RAPD) primers, which were then converted into PCR-based sequence characterized amplified region (SCAR) markers. The CY6069-specific primer set amplified about 550 bp successfully, while the CY6097 marker produced the expected 690 bp band, by which those markers were nominated by CY6069_550 and CY6069_690 SCARs, respectively. Together with the reported morphological and other phenotypic features, the SCAR markers confirmed in this study will be useful to identify those medium-leaf zoysiagrass genotypes when they are cultivated with other vegetatively propagated warm-season turfgrasses in sod farms.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.184-190
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• 2013

It is important for genetic resources to collect and identify in native medium-leaf type zoysiagrasses species distributed in Korea. This study was conducted to investigate morphological variation and characteristics of native medium-leaf type zoysiagrasses from coastal, island and inland regions in Korea. Among them, 75 collected lines was confirmed to have various morphological variations, accessions were classified into 2 main based group coastal and inland regions by morphological characteristics and site environment. Group I included Z. sinica type, this group showed 3.7 mm in leaf width, 29 in number of seed per spikelet and 5.0 mm in seed length. Group II included Z. japonica type, this group showed 4.4 mm in leaf width, 42 in number of seed per spikelet and 3.5 mm in seed length. There is a need for additional research on growth characteristics and the molecular level for the introgressive hybridization between species which confirmed that cross-pollination is possible due to protogyny. The individuals showing variations should be preserved as valuable genetic resources for the expansion of variations in zoysiagrasses, and the results of this investigation on the genetic resources collected will be highly valuable in breeding high quality turfgrass.

• The Korean Journal of Mycology
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• v.23 no.3 s.74
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• pp.257-265
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• 1995

Morphological characteristics and pathogenicity of Rhizoctonia species causing blight diseases of turfgrasses were studied. The species were identified as Rhizoctonia cerealis Van der Hoeven, R. oryzae Ryker et Gooch, and R. solani $K{\ddot{u}hn}$ based on their morphological and cultural characteristics. Isolates of R. solani were assigned to anastomosis groups (AG) with cultural type 1 (1A), 2-2 (IIIB), and 2-2 (IV). R. cerealis, R. oryzae and R. solani induced sheath rot and foliar blight symptoms on creeping bentgrass (Agrostis palustris) and zoysiagrass (Zoysia japonica). Inoculation tests showed that disease severity with isolates of R. cerealis and R. oryzae were more serious to creeping bentgrass than zoysiagrass. AG 1(1A) isolates of R. solani were strongly pathogenic on creeping bentgrass, but moderate to zoysiagrass. AG 2-2 (III) isolates were moderately pathogenic to zoysiagrass, but weakly to creeping bentgrass. AG 2-2 (IV) isolates from zoysiagrass were moderately pathogenic to zoysiagrass, but weakly to creeping bentgrass.

• Korean Journal of Plant Resources
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• v.24 no.2
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• pp.260-266
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• 2011

This study was carried out to control biennial weed which gives a lot of damage to landscaped and early growth of lawns in early spring. The results obtained from the mowing experiment are as follows. (1) Average of occurrence rate of weed species of the total treatments was 17.9%, (2) Occurrence rate of weed species was as 24.7% at 12.5 mm low mowing section. The rate indicates that the lowest weed depression effect was appeared at the lowest mowing among 4 mowing sections. (3) Occurrence rate of weed species was 10.6% at 22.5 mm mowing section. The highest weed depression effect among the 4 mowing sections was observed at the 22.5 mm mowing section. (4) Occurrence rates of weed species were 18.2% and 18.3% at 32.5 mm and 42.5 mm high mowing section, respectively.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.2
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• pp.21-32
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• 2011

The effects of high polymer on the seedling survival were investigated in three major turfgrasses. Twelve treatments were used in the study with different rates of sand, soil organic amendment (SOA), and water-swelling polymer (WSP). Turfgrass seedling survival rate was evaluated in creeping bentgrass (CB), Kentucky bluegrass (KB), and zoysiagrass (Zoy) grown under greenhouse conditions. Significant differences were observed among the treatments. Seedling survival rates were variable in CB, KB, and Zoy according to mixing rates of SOA and WSP, being maximum 20.2% in differences. At 6 weeks after seeding, the survival rates ranged from 0.6 to 61.9% in CB, 4.2 to 75.3% in KB and 1.7 to 82.1% in Zoy. A pattern of seedling emergence varied with time among treatments influenced by WSP rates. A proper mixing rate of WSP is considered to be 5% for CB and 5 to 10% for KB and Zoy. In general, overall effect of WSP on seedling survival was clearly observed in the mixtures of sand 80% and SOA 20% in CB. The best result, however, was found from the mixture of sand 85% and SOA 15% in both KB and Zoy. When mixing sand with WSP, a proper rate of SOA is considered to be 20% for CB and 15 to 20% for KB, while 10 to 15% for Zoy. A further research is needed to investigate the effects of WSP on the turf quality in mixtures of sand, SOA, and WSP before a field application.

• Korean Journal Plant Pathology
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• v.10 no.1
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• pp.54-60
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• 1994

Incidence of Rhizoctonia blight ranged from 22.2% to 100% in the golf courses at six geographical locations in Korea from 1989 to 1993. Rhizoctonia blight occurred more severly in southern area than in northern area. Fifty seven isolates of Rhizoctonia solani obtained from diseased parts of zoysiagrasses were grouped to AG2-2 by anastomosis test. Pathogenicity testes revealed that this pathogen was strongly pathogenic to Korean lawngrasses(Zoysia japonica, Z. matrella, Z. tenuifolia), but not pathogenic to creeping bentgrass(Agrostis palustris), bermuldagrass(Cynodon dactylon), Kentucky bluegrass(Poa pratensis), perennial ryegrass(Lolium prenne), and creeping red fescue(Festuca rubra subsp. rubra L.). The isolation frequency of R. solani AG2-2 fro sheaths of the infected plants was the highest by 91.67%, and that from stolons and roots was 11.13% and 5.63% respectively. The pathogen was not isolated from the leaves. Population density of R. solani in the lawn of large circular patch was highest on surface soils down to 1 cm deep with the value of 4.9$\times$104 (CFU/g soil), but below 1 cm population density decreased sharply down to 0.8~9.8$\times$103 (CFU/g soil). Horizontal distribution of propagules in turfgrass soil was higher in the margin than in center of patch, where the number of propagules was similar to these of healthy looking soils close to the margin of diseased patch. The meteorological factors influencing the outbreak of the disease were temperature, the number of rainy days and precipitation. Optimum temperature for disease development of Rhizoctonia blight in field was 20~22$^{\circ}C$, and that for hyphal growth of R. solani AG2-2 in vitro was 25~3$0^{\circ}C$. In Pusan area, Rhizoctonia blight first occurred in late April and rapidly developed in late June. The disease slightly decreased during July to August and developed again in late September in 1993. The monthly disease progress in Pusan area was similar to that in Kyeonggi province.

• The Plant Pathology Journal
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• v.15 no.2
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• pp.112-118
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• 1999

Eleven species of Pythium were isolated from leaf blight symptoms on creeping bentgrass (Agrostis palustirs Huds.), Kentucky bluegrass (Poa pratenisis L.) and zoysiagrasses (Zoysia japonica Steud., and Z. matrella (L.) Merr.) planted on golf courses in Korea. Mycelial growth on potato carrot agar medium under various temperature conditions indicated that Pythium species obtained in this study could be divided into four groups based on their responses to temperature conditions. P. vanterpoolii was found to favor low temperature conditions with the optimum temperature of $25^{\circ}$, whereas P. aphanidermatum and P. myriotylum favored relatively high temperature conditions with the optimum temperature of $35^{\circ}$. Other species including P. arrhenomanes, P. catenulatum, P. graminicola, P. oligandrum, P. rostratum, P. torulosum, and P. ultimum were the intermediate group with the optimum temperature of 25~$35^{\circ}$. P. periplocum was similar to the intermediate group but the minimum temperature for its mycelial growth was $15^{\circ}$, which was approximately $5^{\circ}$ above that for the intermediate Pythium spp.group. In the pathogenicity tests conducted in the lab using potted plants, P. aphanidermatum, P.a arrhenomanes, P. catenulatum, P. graminicola, P. myriotylum, P. periplocum, P. rostratum, P. torulosum, P. ultimum, and P. vanterpoolii were found to be pathogenic to creeping bentgrass and Kentucky bluegrass. P. aphanidermatum, P. catenulatum, and P. graminicola were frequently isolated from leaf blight symptoms of creeping bentgrass and Kentucky bluegrass in golf courses during the warm and humid periods in July-August. On the other hand, P. vanterpoolii and P. torulosum were frequently isolated during the cool and humid periods in March-May, suggesting both species might be the major causes of leaf blight occurring in the spring time. Zoysiagrass was susceptible to P. arrhenomanes and the heterothallic Pythium sp. (Ht-F), showing stem and crown rot of turf-grasses at poorly drained areas under coool and humid or rainy conditions. P. oligandrum and the heterothallic Pythium sp. (Ht-L) isolated from creeping bentgrass were avirulent to all species of turfgrasses tested in this study.

• Korean Journal of Weed Science
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• v.16 no.3
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• pp.221-229
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• 1996

This study was carried out to determine the effects of growth regulators, carbon sources and silver nitrate on callus formation and plant regeneration of turfgrass. The results were summarized as fallows : Callus from Korean lawngrass (Zoysia japonica Steud.) and pencross creeping bentgrass (Agrostis palustrir Huds.) induced better in MS medium than in N6 medium and by addition of 2,4-D than by that of NAA. Callus formation from Korean lawngrass and penncross creeping bentgrass was very effective at MS medium adding 1mg/L 2,4-D and at the medium adding 2mg/L 2,4-D, repectively. Growth of callus was good at MS medium containing 2mg/L 2,4-D+0.2mg/L NAA. Callus growth of Korean lawngrass and penncross creeping bentgrass was good when kinetin was added 0.2mg/L and 0.3mg/L, individually, to MS medium containg 2mg/L 2,4-D+0.2mg/L NAA. Regeneration rate from leaf and stock callus of Korean lawngrass was 44% at MS medium adding 2,4-D 2mg/L+NAA 0.2mg/L+kinetin 0.3mg/L and 32% at the medium containing 2,4-D 2mg/L+NAA 0.2mg/L+kinetin 0.3mg/L, each and that from leaf and stock callus of penncross creeping bentgrass was 80% and 67%, each, at the medium adding 2,4-D 2mg/l+NAA 0.2mg/L+kinetin 0.3mg/L. Regeneration rate of Korean lawngrass and penneross creeping bentgrass increased by 3 to 4% and by 10 to 16%, respectively, when added $AgNO_3$ 1~2mg/L to the above-mentioned regeneration medium.

• Asian Journal of Turfgrass Science
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• v.20 no.1
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• pp.33-40
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• 2006

Many soil amendments have been used nowadays to improve physical and chmical condition of turf soil, which might ultimately optimize turfgrass growth in golf courses. This study was carried out to Investigate the effects of new organic soil amendment containing pig excreta 50% and sawdust 50% on growth of zoysiagrass (Zoysia japonica L.) and kentucky bluegrass (Poa pratensis L.) in greenhouse. Three applicable treatments with soil mixtures of 10, 20, and 30% (v/v) animal organic soil amendment (AOSA) with sand, were tested for chemical property, physical property, visual quality and root length of zoysiagrass and Kentucky bluegrass. As results, application of $10{\sim}30%$ AOSA mixtures were proper to grow turfgrass in soil nutrition. Especially, the treatment with 20% AOSA mixtures showed 0.7% in organic matter, which meets to green standard of USGA. Also, 30% AOSA mixtures was 1.1% in organic matter, which might be desirable for zoysiagrass-planted golf courses in Korea. It was turned out that addition of AOSA decreased the hydraulic conductivity in soil physical property Because the sand possess high hydraulic conductivity, it is recommended to combine $10{\sim}30%$ AOSA with sand in order to sustain soil balance. The treatment with $10{\sim}30%$ AOSA noticeably increased visual quality of both zoysiagras and Kentucky bluegrass during 90 days. However, treatments with either 20% or 30% AOSA were effective to develop root length of zoysiagrass but treatments with 20% AOSA were more effective than that of 30% AOSA mixtures to promote root length of Kentucky bluegrass at 60 days. In conclusion, considering all vital factors such as visible quality, root growth, organic matter content, and economical efficiency, was taken, it is recommended that a $20{\sim}30%$ mixture of AOSA with sand is good for the growth of zoysiagrass and 20% mixture for Kentucky bluegrass.