• Title/Summary/Keyword: tuberous roots

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In vitro Formation of Tuberous Roots from Root Ends in the Rooted Tuberous stem without shoots in Cyclamen persicum MILL.

  • Lim, Jong-Gu;Junzo, Fujigaki
    • Plant Resources
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    • v.7 no.3
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    • pp.222-225
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    • 2004
  • In Japan, propagation of cyclamen is mainly from seedlings. However, seeds are expensive and germination is slow and non..uniform. Therefore, to achieve genetically uniform propagation, multiplication must be vegetative. The rooted tuberous stems without shoots as sources of explants were cultured on the media containing BA and sucrose. After 30 days cultivation, tuberous roots were produced from the root ends attached to a tuberous stem and its capability was dependent on the type of media. The highest percentage of tuberous root formation was observed in Culture on the medium of 1/3 MS containing 0.05mgL$^{-1}$ NAA, 0.5mg L$^{-1}$ BA and 5% sucrose. Growth rates of the tuberous roots were greatly influenced by the cutting positions of a root in explants. The highest growth of was observed if small amount of root end was cut at initiation of tissue culture.

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Response of Different Seedlings to Growth and Yield in Yacon

  • Ryu, Jeom-Ho;Doo, Hong-Soo;Lee, Kang-Soo;Choi, Sun-Young;Cheong, Young-Keun;Park, Ki-Hun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.5
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    • pp.356-360
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    • 2002
  • The seedlings of yacon (Polymnia sonchifolia Poeppig & Endlicher), which were cuttings, plug seedling (PS), crown bud before sprouting (CBBS), crown bud after sprouting (CBAS), and divided seedling after budding (DSAB) were planted at 70$\times$50 cm planting distance on ridge; that was interow spacingintrarow spacing, and about 28,500$\pm$71 plants/ha. CBBS didn't need work and equipment to raise seedlings. PS and DSAB grew taller to 140.5 and 143.3cm, respectively, than others at 150 days after planting. In the changes of plant height, PS and DSAB showed taller than others during growth period, cuttings, CBBS, and CBAS grew rapidly in middle growth stage. Excepting main stem and petiole length, other characters were significant for seedling. Fresh weights were different among seedlings. Even though the yield of plants grown from CBAS and CBBS were lower with 34.7 and 36.4 ton/ha, respectively, than 3.6 ton/ha of DSAB; its yield index were over 95%, hence, those of plants grown from cutting and PS were lower with 73 and 87%, respectively. The ratio of tuberous roots over 200g to total tuberous roots per plant was the highest from DSAB. Most of tuberous roots were under 200g per tuberous root from cuttings. CBBS, CBAS, and DSAB are suitable to use seedlings for high yield of yacono. Yacon plant by DSAB much produced tuberous root of over 200g.

Changes in Sugar Contents and Storability of Yacon under Different Storage Conditions

  • Doo, Hong-Soo;Li, Hu-Lin;Kwon, Tae-Oh;Ryu, Jeom-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.5
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    • pp.300-304
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    • 2000
  • Tuberous roots of yacon (Polymnia sonchifolia Poeppig & Endlicher) were stored in different temperature and relative humidity conditions after curing for 30 days. Non-decadent percentage, moisture contents, brix degree and sugars were investigated. Whether temperature and relative humidity were high or not, most of the tuberous root decayed during storage, and the decayed percentage was increased as long as the storage period. In final, only 13% of tuberous roots remain intact at 4$^{\circ}C$, 85% relative humidity condition to be appeared best storage condition in this experiment and all of the tuberous root decayed in other storage conditions after 6 months. Moisture contents decreased a little in the high relative humidity. Even though tuberous roots decayed when stored at 1$0^{\circ}C$ and 2$0^{\circ}C$, most of brix degree reached about 17.0. Fructose, glucose and sucrose contents were 1.65, 1.15 and 0.35% at early storage period, and 1.6, 1.1 and 0.5% after 6 months at 4$^{\circ}C$, 85% relative humidity, respectively. In 3 temperature conditions, fructose and glucose increased for a month and then decreased successively afterwards, but sucrose increased gradually during 6 months. Also in 4 relative humidity conditions, fructose, glucose and sucrose contents were changed with similar to that of in temperature. Changes of fructose and glucose were the same except on 4$^{\circ}C$, 85% relative humidity, that was lower level than moisture of tuberous root.

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Effect of Reciprocal Grafting on Growth and Yield of Sweet potato (고구마 품종간 이면접목이 생육과 수량에 미치는 영향)

  • 남상영;정승근;김인재;김민자;이철희;김태수
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.3
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    • pp.176-179
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    • 2001
  • To obtain the basic information of high yielding ability for varietal improvement and cultural practice, reciprocal grafts were made analyzed among five sweet potato cultivars. Five cultivars were characterized with the different in whose source and sink capacities. Grafting decreased main vine length, number of tuberous roots per plant, but its effects were different among grafting combinations. The growth of vines was affected more by scion, while growth and yield of under-ground were influenced more by stock. Grafting effects, interactions between stock and scion for main vine length and number of tuberous roots were highly significant in diallel grafting. The percentage of marketable tuberous roots was higher in the order of stem cutting>self grafting>reciprocal grafting.

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Effect of Plant Density on Growth Responses and Yield in Yacon

  • Ryu, Jeom-Ho;Doo, Hong-Soo;Lee, Kang-Soo;Park, Sun-Young
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.5
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    • pp.407-410
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    • 2001
  • This study was conducted to determine the optimal planting distance in cultivation of yacon (Polymnia sonchifolia Poeppig & Endlicher). Plug seedlings were planted with 6 different plant densities of 80 $\times$ 60, 80 $\times$ 50, 70 $\times$ 50, 70 $\times$ 45, 70 $\times$ 40 and 60 $\times$ 45 cm. The plant height and the petiole length were increased with increasing the planting distance. The tallest plant height of yacon was 165.4cm with the plant density of 80 $\times$ 60 cm. However, branch number per plant, leaf number on main stem and stem diameter were not significantly difference among planting densities. Tuberous root was harvested 31.42 tons/ha in 70 $\times$ 50 cm spacing. The ratio of heavier tuberous roots than 200 g to total tuberous roots decreased significantly according to increase of planting density. Fresh weights of shoot and root, contain the crown bud, were decreased, as planting distance was shorter. Tuberous root number was fewer but its weight was heavier in wide planting than in dense planting. We think that optimal planting density is about 30,000 plants/ha, if it were to be 70 cm row spacing, intrarow spacing should calculate about 47cm.

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Effect of Transplanting Date on Growth and Yield of Yacon (정식시기가 야콘의 생육 및 수량에 미치는 영향)

  • 강영길;고미라
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.3
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    • pp.188-193
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    • 2004
  • Fifty-day-old plug seedlings of yacon (Polymnia sonchiflolia Poeppig & Endlicher) were transplanted on Mar. 23, Apr,8, Apr,23, May 8, May 23, and June 7,2002 at Jeju to evaluate the effect of transplanting date on the growth and yield of yacon in Jeju island. Delaying the transplanting until May 23 greatly decreased plant height stem height, main stem diameter and number of nodes per plant. The number of marketable tuberous roots was about 6.0 per plant with the early transplanting from March 23 to April 231 but decreased to 1.9 per plant with the latest transplanting time, June 7. The yield of marketable tuberous roots of early transplanted (March 23 to April 23) yacon was about 27 MT/ha, and was 32.4 MT/ha at April 23 transplanting, but was remarkably reduced at the three later transplantings, May 8, May 23, and June 7. Dry matter and soluble solid contents of marketable tuberous roots were 14% and 15%, respectively, regardless of transplanting date. The weight of a marketable tuberous root ranged from 153 to 183.9 g at the first three transplantings, but gyeatly reduced with delaying transplanting until May 7. The optimum transplanting date for yacon in Jeju island seems to be from mid-April to late April considering tuberous root yields, the risk of late-spring frost, and cost of seedling raising.

Development of transgenic sweet potato producing human lactoferrin (인체 락토페린 생산 형질전환 고구마 개발)

  • Min, Sung-Ran;Kim, Jae-Wha;Jeong, Won-Joong;Lee, Young-Bok;Liu, Jang R.
    • Journal of Plant Biotechnology
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    • v.36 no.3
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    • pp.224-229
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    • 2009
  • Human lactoferrin is an iron-binding glycoprotein with many biological activities, including the protection against microbial and virus infection and stimulation of the immune system. We introduced a human lactoferrin (hLf) cDNA under the control of 35S promoter into sweet potato by particle bombardment. Transgenic plants were regenerated via somatic embryogenesis. Transgenic plants were produced typical tuberous roots in soil. PCR, Southern and northern analyses confirmed that the hLf cDNA was incorporated into the plant genome and was properly expressed in plants. Western blot analysis showed that the 80 kDa full length hLf protein was produced in transgenic tuberous roots. Overall results indicated that sweet potato would be an excellent host to produce human therapeutic proteins.

A Comparative Study on the Flowery Knotweed Root and Auriculate Swallowwort Root in Medical Texts (하수오(何首烏)와 백수오(白首烏)에 대한 문헌적(文獻的) 비교(比較) 고찰(考察))

  • Kim, Yong-Ul;Keum, Kyung-Soo
    • Journal of the Korean Institute of Oriental Medical Informatics
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    • v.15 no.2
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    • pp.77-91
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    • 2009
  • Flowery Knotweed Root is the dried tuberous root of the knotweed family wheres Auriculate Swallowwort Root is the dried tuberous root of the swallowort family. Flowery Knotweed Root is also called Red Flowery Knotweed Root in China, which is dried root of Polygonum multiflorum THUMB. Chinese Flowery Knotweed Root is better known as the dried tuberous root belonging to the family Apocynaceae whereas White Flowery Knotweed Root in Korea is the dried tuberous root of Cynanchum wilfordii(Maxim) Hemsl. Up to now, while the dried root named Red Flowery Knotweed Root has been widely used in China, Auriculate Swallowwort Root has been widely used in Korea. Both the roots contrast in a striking way with the origin, shape, botanical names, other names, and chemical constituents, so administration and dosage without discrimination of both sides can be open to question. According to the literature, it is recorded that Flowery Knotweed Root and Auriculate Swallowwort Root are similar to the characteristics, properties, and actions. From this study, the result is as follows: 1. Flowery Knotweed Root and Auriculate Swallowwort Root are divided into the family Apocynaceae and the family Polygonaceae, respectively. Accordingly 2. Flowery Knotweed Root is in shape of an irregular spindle and looks reddish- brown or deep reddish-brown and the section is light yellowish-brown, while Auriculate Swallowwort Root looks yellowish-brown and the section is white or yellow. 3. Flowery Knotweed Root is reported by the Journal of the Crude Drug that the medicinals have not the same therapeutic action as Auriculate Swallowwort Root. It is recorded that the properties, channel entry, functions of Flowery Knotweed Root and Auriculate Swallowwort Root in literature have much in common. Many works on the roots of this plant has been used traditionally as a tonic are required of. In view of the results, Flowery Knotweed Root and Auriculate Swallowwort Root varies both in nature and in shape, so that the administration and dosage of the medicinals must be taken a prudent attitude.

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Classification of Sweet Potato Varieties Based on Esterase Isozymes and Protein Patterns (Esterase 동위효소 및 단백질 패턴에 의한 고구마 품종 분류)

  • Kim, Deog-Su;Oh, Sung-Kun;Chin, Moon-Sup;Ryu, Jeom-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.3
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    • pp.332-339
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    • 1996
  • Electrophoretic method was utilized to classify 100 varieties of sweet potato germplasm maintained at the National Crop Experiment Station of Korea in 1993. The esterase isozyme patterns in the leaves were classified into 14 different types. Type Ⅸ included the most of the varieties (46) tested and Ⅶ, I, III, Ⅷ, II and V types of all included 47 varieties in order. The other 7 varieties had different band pattern with each other. Type I having many kind of band pattern included Shinyulmi, Beniastma and High starch which had the dry type of tuberous roots varieties. The esterase isozymes pattern in the tuberous roots were classified with 18 kinds of types. The C type included 22 varieties and B, K, A, E, I and N in order. The proteins pattern in the tuberous roots were classified with 7 kinds of types. I type included 36 varieties, and IV type included 27 varieties and II, III, Ⅶ and Ⅵ types in order.

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Effect of Propagation Method, Planting Density, Amount of Nitrogen Fertilizer and Cropping Years on Growth and Yield of Asparagus Cochinchinensis (Lour.) Merr (번식방법, 재식밀도, 질소시비량 및 재배년수가 천문동의 생육과 수량에 미치는 영향)

  • Kim, Dae-Hyang;Park, Choun-Bong;Kim, Jong-Yeob
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.2
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    • pp.93-97
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    • 2010
  • Cultural practices of Asparagus cochinchinensis in highland area were performed for a potential medicinal crop. These studies were examined to propagation methods, planting densities, nitrogen treatments, and cropping years. The results are summarized as follows. The adequate number of buds per tuberous root was 4 for vegetative propagation because the number of tuberous root harvested was 16.8 and the yield was also the highest, exhibiting 1,060 kg/10a. The suitable planting time for vegetative propagation was later than early April. If the earlier tuberous roots were planted, the less they emerged. The highest emergence rate was obtained from the planting density of $30{\times}20cm$ as 97.2% while the yield was highest in the $30{\times}15cm$ density, exhibiting 1,883 kg/10a with emergence rate at 94.9. It seemed that the higher planting density promoted plant height growth and yield in Asparagus cochinchinensis. The highest fresh weight was recorded at 6 kg/10a of nitrogen fertilizer into the sandy loam soil compared to the level of 0, 3, 9 kg/10a. The yield was increased with cropping years. However, the proper harvesting time was the second year of cultivation because the rate of weight increase was maximized in the 2-year-old tuberous root. The yield in the third year was decreased as compared to that of the second year.