• Title/Summary/Keyword: tuberous root

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A Case Report of Imports Morphological Variation of Pinelliae Tuber Based on the Genetic Analysis (유전자 분석 기반 수입산 형태 변이 반하 유통 사례 보고)

  • Kim, Wook Jin;Choi, Goya;Noh, Sumin;Moon, Byeong Cheol
    • The Korea Journal of Herbology
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    • v.37 no.5
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    • pp.9-16
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    • 2022
  • Objectives : The purpose of this study is to report that applying the genetic discrimination method to Pinelliae Tuber is suitable as a countermeasure for the limitations of morphological identification announced publicly in the Ministry of Food and Drug Safety(MFDS). Methods : Randomly selected fifty samples in Pinelliae Tuber imported from China were used for morphological and genetic identification. The morphological identification was applied method announced publicly by the MFDS. The traits of morphological identification were classified as Pinellia ternata, P. tripartita, Pinellia pedatisecta, and Typhonium flagelliforme, according to the formation of tuberous root and tuber morphology. The genetic identifications were conducted by Sequence Characterized Amplified Region(SCAR) marker and DNA barcoding analysis for cross-validation, respectively. SCAR marker was verified according to the presence or absence of amplicon through PCR amplification using species-specific primers. DNA barcoding analysis used sequence information of the matK region. Results : As a result of the morphological identification, 27 out of 50 samples were identified as original species 'P. ternata' of genuine 'Pinelliae Tuber', and 23 were identified as adulterant species 'P. pedatisecta'. Unlike this, the genetic identification was identified as the original species 'P. ternata' in all 50 samples in the SCAR marker and matK regional sequence analysis. Conclusions : Pinelliae Tuber of morphological mutant that can not be classified by morphological identification is imported from China. The SCAR marker would be used as accurate and efficient assays for species identification of the morphological mutant.

Effects of Varieties, Growth Duration, and Topping for Occurrence of Skin Ridge on the Tuberous Root in Sweet Potato (Ipomoea batatas Lam.) (고구마 품종, 생육기간 및 경엽제거가 피맥발생에 미치는 영향)

  • Choi, Kyu-Hwan;Yu, Young-Jin;Kim, Hyo-Jin;Kang, Chan-Ho;Jeong, Jong-Sung;Song, Young-Ju;Kim, Chung-Kon;Lee, Seung-Yeop
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.2
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    • pp.194-200
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    • 2014
  • Skin ridge (SR) is a irregular line shape that stand out in epidermal layer on sweet potato (Ipomoea batatas Lam.). SR reduced marketable value of storage root in sweet potato. It would be occurred by accumulation of starch in epidermal layer. SR rate was more highly increased in early planting. SR rate was under 3% in 7 varieties 'Geonpoongmi', 'Yeonhwangmi', 'Geonmi' etc and the rate of two varieties 'Healthymi', 'Borami' were up to 30%. SR rate was positively correlated with vine weight. SR rate was the highest in 120 days after planting. As leaf area index was reduced to 3, 4, 5 and 6 (non-topping) by topping, it was hard to fine SR occurrence in topping treated sweet potato. Topping treatment, however, caused decrease in yield overall. Therefore, topping treatment for decreasing of SR was not recommended in agricultural practice of sweet potato.

Antioxidative and Biological Activites of Extracts of Sweetpotato Tips (고구마 끝순 추출물의 항산화 및 생리활성)

  • Lee, Joon-Seol;Park, Yang-Kyun;Ahn, Young-Sup;Kim, Hag-Sin;Chung, Mi-Nam;Jeong, Byeong-Choon;Bang, Jin-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.52 no.2
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    • pp.228-238
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    • 2007
  • This study was conducted to increase sweetpotato utilization and to determine the vegetative value of sweetpotato tips by investigating the phenolic compounds, antioxidative effect in oil, electron donating ability, nitrite scavenging effect and ACE inhibition activities. The phenolic compounds present in sweetpotato tips are the gallic, chlorogenic, gentisic, caffeic, couramic and ferulic acid, which are 16-122 times higher compared to other vegetables such as spinach, soybean sprout, and perilla leaves. In each solvent extract, the total phenolic compounds (175.8mg/g) was composed of 55% EtOAc extraction and 39% BuOH extract, respectively. The results of induction period using the Rancimat method showed that the antioxidant activity of SP tips was higher than the tocopherol or BHT. The relative levels of each solvent extract in SP tips were as follows: EtOAc>BHT>BuOH>Tocopherol>Water>$CHCl_3$>Hexane. The peroxide value was measured every 5 days for 25 days during storage and results showed that the peroxide value, the tips, tuberous root and tocopherol were lower compared to spinach, soybean sprout and perilla leaves. Nitrite scavenging effects were excellent in sweetpotato tips, perilla leaves and soybean sprout, especially, inhibition rate of perilla leaves (72%) were superior to the others. In process of solvent extraction, activity of BuOH and water extractions were the best. ACE inhibition activity in sweetpotato tips was 1.5 times higher than in tuberous roots and $1.9{\sim}3.7$ times higher than in spinach, soybean sprout, perilla leaves.

Antioxidative and Biological Activites of Extracts of Sweetpotato Tips (고구마 끝순 추출물이 알코올 투여 흰쥐의 항산화 효소계 및 지질과산화에 미치는 영향)

  • Lee, Joon-Seol;Park, Yang-Kyun;Ahn, Young-Sup;Kim, Hag-Sin;Chung, Mi-Nam;Jeong, Byeong-Choon;Bang, Jin-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.52 no.4
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    • pp.411-420
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    • 2007
  • This study was conducted to increase sweetpotato utilization and to determine the vegetative value of sweetpotato tips by investigating the phenolic compounds, antioxidative effect in oil, electron donating ability, nitrite scavenging effect and ACE inhibition activities. The phenolic compounds present in sweetpotato tips are the gallic, chlorogenic, gentisic, caffeic, couramic and ferulic acid, which are 16-122 times higher compared to other vegetables such as spinach, soybean sprout, and perilla leaves. In each solvent extract, the total phenolic compounds(175.8 mg/g) was composed of 55% EtOAc extraction and 39% BuOH extract, respectively. The results of induction period using the Rancimat method showed that the antioxidant activity of SP tips was higher than the tocopherol or BHT. The relative levels of each solvent extract in SP tips were as follows: EtOAc>BHT>BuOH>Tocopherol>Water>$CHCl_3$>Hexane. The peroxide value was measured every 5 days for 25 days during storage and results showed that the peroxide value, the tips, tuberous root and tocopherol were lower compared to spinach, soybean sprout and perilla leaves. Nitrite scavenging effects were excellent in sweetpotato tips, perilla leaves and soybean sprout, especially, inhibition rate of perilla leaves(72%) were superior to the others. In process of solvent extraction, activity of BuOH and water extractions were the best. ACE inhibition activity in sweetpotato tips was 1.5 times higher than in tuberous roots and $1.9{\sim}3.7$ times higher than in spinach, soybean sprout, perilla leaves.

Effects of Polyethylene Mulch Color and its Removing Time on Growth and Yield of Yacon (Polymnia sonchifolia Poepp. & Endl.) (멀칭비닐 색상과 제거시기가 야콘(Polymnia sonchifolia Poepp. & Endl.)의 생육 및 수량에 미치는 영향)

  • Doo, Hong-Soo;Moon, Jung-Kil;Kwon, Tae-Oh;Ryu, Jeom-Ho
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.3
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    • pp.205-212
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    • 1999
  • This study was carried out to examine the effect of polyethylene film color and removing time of the mulching film in yacon (Polymnia sonchiiolia Poepp. & Endl.). The plant height on nonmulching was smaller about 10 cm or larger with 112cm than on other mulches ranged with $134{\sim}139$ cm at harvesting, and other characters examined were similar to that of plant height. The weight of tuberous root per plant was heavier on mulches ranged with $0.79{\sim}0.91$ kg than on non-mulching with 0.61 kg. Among the kind of mulches, plant height was the tallest with 139 cm in combination and declined in order of transparent, green and black polyethylene films. Yields among colored polyethylene films were the heaviest with 0.91 kg per plant in combination, 0.88 kg in black and 0.84 kg in green. In growth and yield full mulching untill harvesting was more effective than the other removed mulching treatments. It was effected on growth promotion at early growth stage, soil temperature and water preservation.

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The morphology of Thalictrum L. in Korea (한국산 꿩의다리속(Thalictrum L.) 식물의 형태학적 연구)

  • Park , Seong-Jun;Park, Seon-Joo
    • Korean Journal of Plant Taxonomy
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    • v.38 no.4
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    • pp.433-458
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    • 2008
  • This study were examined for their external morphological characters such as root, leaf, inflorescenses, flower, stamen, pistil, and fruit as to Korean Thalictrum L. and discussed taxanomic value of characters, and made out a new key. This study confirmed that specialized stems and roots type were divided into fibrous type, fibrous having a tuber type, long fusiform tuberous type, tuberoid type, rhizome type and stolon type. The stem were divided into two types based on existence and nonexistence of striate or pubescent. Leaves were usually 2-3-ternately and was divided into three types based on leaf apex, leaf base and number of lobe. Inflorescenses were divided into two type; corymb, panicle. Flower is bisexual, and don't have petal. It was divided from existence and nonexistence of calyx when it flowering. Especially, the stamens were divided from anther shape and apex shape, degree of dilation of filament, epidermic shape of filament etc., and the pistils were divided from glandular on ovary, length of carpel stipe etc.. The fruit is a achene and it was divided from achene shape, achene rib or wings, achene stipe length. This study suggest that useful characters for identifying section are degree of dilation of filament, anther shape, achene rib and wings, degree of dilation of achene. Also, roots, leaf shape, stamen and pistil shape, achene beak and achene length, achene and leaf hair appear to be useful characters for identifying species.

The Effect of Ampelopsis japonica (Thunb.) Makino on Osteoclastogenesis and Expression of Osteoclast-Related Gene (백렴(白蘞)의 파골세포 분화 및 관련 유전자 발현 억제에 미치는 영향)

  • Hongsik Kim;Sumin Lee;Minsun Kim;Jae-Hyun Kim;Yejin Kang;Seoung Jun Kwon;Youngwoo Nam;Seungwoo Yoo;Hong-Seok Choi;SeonJin Huh;Youngjoo Sohn;Hyuk-Sang Jung
    • The Korea Journal of Herbology
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    • v.38 no.5
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    • pp.9-19
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    • 2023
  • Objectives : Osteoporosis is a systemic skeletal disorder characterized by reduced bone mineral density and increased risk of fractures. Bisphosphonates and selective estrogen receptors, which are bone resorption inhibitors that are currently widely used as osteoporosis treatments, show serious side effects when administered for a long time. Research on bone resorption inhibitors that complement the problems of existing treatments is needed. The purpose of this study was to investigate the effect of inhibiting osteoclast differentiation and activity on the tuberous root of Ampelopsis japonica (Thunb.) Makino (AM). Methods : After extracting AM using distilled water and ethanol, the inhibitory effects of the two solvents on osteoclast differentiation were compared using the RANKL-induced in vitro experimental model and the TRAP assay kit. The impact of AM on bone resorption was investigated through the pit formation assay, and its effect on F-actin formation was assessed through fluorescent staining. Additionally, protein and mRNA expression levels of osteoclast differentiation markers (NFATc1, c-Fos, TRAP and ATP6v0d2) and resorption markers (MMP-9, CTK, and CA2) were analyzed via western blot and RT-PCR. Results : AM treatment significantly decreased the number of TRAP-positive cells and pit formation area. Furthermore, AM suppressed both the protein and mRNA expression of NFATc1 and c-Fos, key transcription factors involved in osteoclast differentiation and it downregulated the expression of osteoclast-associated genes such as TRAP, CTK, MMP-9, CA2, and ATP6v0d2. Conclusions : These results suggest that AM can inhibit bone resorption and osteoclast differentiation, indicating its potential for use in the treatment and prevention of osteoporosis.

Comparison of Nutrient Composition of Yacon Germplasm (야콘 유전자원의 영양성분 비교 분석)

  • Kim, Su Jeong;Jin, Yong Ik;Nam, Jeong Hwan;Hong, Su Young;Sohn, Whang Bae;Kwon, Oh Kuen;Chang, Dong Chil;Cho, Hyun Mook;Jeong, Jin Cheol
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.9-18
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    • 2013
  • The aim of this study is to provide the basic data for yacon [Samallanthus sonchifolius (Poepp. & Endl.) H. Robinson] in dietary food. The nutritional compositons, such as protein, ash, carbohydrate, dietary fiber, vitamin and fructooligosaccharide, were analyzed for 4 yacon germplasm lines. Yacon has low calories with only 46~56 kcal/100 g. The contents of water, fat, ash, protein, carbohydrate and dietary fiber were ranged 85.9~86.8%, 0.1~0.2%, 0.2~0.3%, 0.5~0.7%, 12.2~13.1% and 1.05~1.14%, respectively. The iodine-starch test did not show any color or precipitation reaction, which indicates that yacon has no starch content. However, in the absence of starch, yacon is rich in fluctooligosaccharide, which is between 9.6~11.1%. Maltose is present in the larger amount, followed by sucrose, glucose, and fructose in terms of free sugars. The analysis of minerals revealed that yacon contains potassium in the larger amount of 141~176 mg/100 g F.W., followed by magnesium at 8.2~10.6 mg, calcium, and sodium representing the least present mineral. Yacon proved to have a total of 17 types of amino acids, which are between 404.0~581.8 mg per 100 g of yacon. Glutamic acid, the main sweetening component, is present in the large amount of 94.0~182.2 mg/100 g F.W., followed by aspartic acid, arginine, and alanine. The proportion of the essential amino acid was 24.8~33.6%. Results of analysis also showed that yacon contains 0.001~0.024 mg, 0.03~0.11 mg, 0.02~0.3 mg, 0.3~0.4 mg and 14.1~20.6 mg of ${\beta}$-carotene, thiamin, riboflavin, niacin, and ascorbic acid, respectively. It is also likely to be highly used as functional food material in the future because it is abundant in both fluctooligosaccharide and antioxidants which are important functional components.

Principal Component Analysis of the Classification of Yacon Cultivation Areas in Korea (주성분 분석을 이용한 야콘의 재배지대 구분)

  • Kim, Su Jeong;Sohn, Hwang Bae;Hong, Su Young;Nam, Jung Hwan;Chang, Dong Chil;Kim, Ki Deog;Suh, Jong Taek;Koo, Bon Cheol;Kim, Yul Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.2
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    • pp.149-155
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    • 2017
  • To establish cultivation areas for the stable production of yacon, this study investigated the productivity and functional component contents of yacon in eight regions of Korea from 2011 to 2013. The results of principal component analysis using these data were as follows. A survey of 16 agricultural traits and meteorological data in the eight yacon cultivation areas showed that five factors (average temperature, maximum temperature, minimum temperature, frost-free days, and fructooligosaccharide content) were highly significant at the p < 0.001 level. Among the 16 agricultural traits and meteorological data used in the main component analysis of yacon cultivation areas, approximately eight contributed to the first principal component, and approximately four contributed to each of the second and third principal components. In particular, factors related to productivity, fructooligosaccharide content, and temperature change were considered important criteria for the classification of cultivation areas. The cultivation areas were divided into three groups by principal component analysis. In Group I, containing the Jinbu and Bonghwa areas in the mid-highland region at 500-560 m above sea level, the product yield was the highest at 2,622-3,196 kg/10a, the fructooligosaccharide content was also the highest at 9.04-9.62%, and the mean temperature was $17.3-18.5^{\circ}C$. In Group II, the areas Suncheon, Okcheon, Yeoju, and Gangneung, at 20-180 m above sea level, had the lowest yield, relatively lower fructooligosaccharide content, and the highest temperature. The areas in Group III showed values intermediate between those of Group I and Group II. For the different yacon cultivation areas, the product quantity and fructooligosaccharide content differed according to the environmental temperature, and the temperature conditions and number of frost-free days are considered important indicators for cultivation sites. Therefore, in terms of producing yacon with high quality, cultivation at 500-560 m is considered to give a higher yield and functional fructooligosaccharide content.