• Korean Journal of Plant Tissue Culture
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• v.24 no.2
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• pp.77-81
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• 1997

For the early selection of heat-tolerant clones, the true potato seeds of 750 clones were obtained by several cross combinations : $CIP\;575015\;{\times}\;katahdin,\;CIP\;575015\;{\times}\;B6603-6,\;84\;I\;35-4\;{\times}\;katahdin,\;CIP\;575015\;{\times}\;NookSack,\;and\;CIP575015\;{\times}\;Superior.$ The ratios of in vitro tuberization at 3$0^{\circ}C$ were decreased by 43% in all cross combinations compared with at 2$0^{\circ}C$. In particular, tuberization rate of $CIP575015\;{\times}\;katahdin$ cross at 3$0^{\circ}C$ was only 21%. On the other hand, the rate of tuberization of $CIP575015\;{\times}B6603-6$ cross was 58 % at 3$0^{\circ}C$, so this cross combination was thought to be good heat-tolerant clone. To screen the heat-tolerant clones in lower land during high temperature period, microtubers were cultivated in Kangnung, and the characters of tubers and productivity were examined. Among screened clones, one clone at 2$0^{\circ}C$ and three clones at 3$0^{\circ}C$ were shown to be heat-tolerant. The yield of 89ML75-8 was 88% more than that of Superior, and dry weight rate of 89 ML64-11 was 18.8%. Therefore, 89ML64-11 clone was considered as a good cultivar for processing.

• Korean Journal of Weed Science
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• v.11 no.1
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• pp.60-67
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• 1991

The experiment was conducted to evaluate weeding effect and to inhibit tuberization of Eleocharis kuroguwai authority by single or systematic applications of several herbicides. E. kuroguwai was controlled above 90 percentage by pretilachlor and butachlor+bensulfuron application at 5-10 DAT(Days after transplanting) followed by bentazon at 50 DAT and weeded 88 percentage by mefenacet+bensulfuron application at 15 DAT. Tuberization inhibition rate of E. kuroguwai was higher at the application of butachlor+bensulfuron at 5 DAT followed by bentazon at 50 DAT than any other applications. The weight variation of E. kuroguwai tubers formed at 15-20cm soil stratum was larger than that at 10-15cm. Tuber of E. kuroguwai was hardly formed at top soil stratum (0-5cm) and deep soil (15-20cm) by the systematic application of bentazon at 50 DAT. Grain yield of rice from systematic application with bentazon at 50 DAT and mefenacet+bensulfuron at 10-15 DAT was similar to that of hand weeded plot.

• Korean Journal of Weed Science
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• v.17 no.1
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• pp.10-23
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• 1997

This study was carried out to determine distribution and tuberization characteristics of tubers of water chestnut(Eleocharis Kuroguwai Ohwi) which is a dominant weed species and diffult to control during 1993 to 1994. By early planting of E. kuroguwai tubers, more and heavier tubers were developed, but the tubers were tended to distribute at the upper soils. Large proportion of tubers was remained at the upper 0 to 10cm soil layer and a few tubers were formed below 20cm. Tubers developed earlier tended to be at deeper layer, while later developed tubers were at upper layers. Tuber weight was increased from the surface to 20cm soil depths, but that formed below 20cm was almost same. No tillage resulted in more tuber formation which were distributed at upper soil layers when compared to conventional tillage. Cool water irrigation pumped from ground water resulted in less tuberization but smaller tubers when compared to control. Shading with color cellophan films resulted in smaller tuber formation with lower in sprouting percentage. Among the films tested, the most significant effect was obtained with green color.

• Journal of Plant Biotechnology
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• v.49 no.1
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• pp.39-45
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• 2022

Conserved domains are defined as recurring units in molecular evolution and are commonly used to interpret the molecular function and biochemical structure of proteins. Herein, the ADP-glucose pyrophosphorylase (AGPase) amino acid sequences of three species of the Ipomoea genus [Ipomoea trifida, I. triloba, and I. batatas (L.) Lam. (sweetpotato)] were identified to investigate their physicochemical and biochemical characteristics. The molecular weight, isoelectric point, instability index, and grand average of hyropathy markedly differed among the three species. The aliphatic index values of sweetpotato AGPase proteins were higher in the small subunit than in the large subunit. The AGPase proteins from sweetpotato were found to contain an LbH_G1P_AT_C domain in the C-terminal region and various domains (NTP_transferase, ADP_Glucose_PP, or Glyco_tranf_GTA) in the N-terminal region. Conversely, most of its two relatives (I. trifida and I. triloba) were found to only contain the NTP_transferase domain in the N-terminal region. These findings suggested that these conserved domains were species-specific and related to the subunit types of AGPase proteins. The study may enable research on the AGPase-related specific characteristics of sweetpotatoes that do not exist in the other two species, such as starch metabolism and tuberization mechanism.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.4
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• pp.418-423
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• 2012

Field experiments were carried out to improve the cultural practice of potato by analysing its tuberization patterns. Tuberization patterns affected by different altitudes was analyzed at two potato cultivation regions, Jinbu (600 m) and Daegwallyung (800 m) using two potato cultivars, 'Superior' and 'Atlantic'. To analyse tuberization patterns affected by different planting time, seed potatoes were planted at every 10 days from April 19 to May 19 in Daegwallyung. Total dry weight was greater in plant grown at the altitude of 800 m than 600 m during the entire growth period and the highest increase was observed at the early growth period, July 6, comparing to other growth period. The total dry weight was the greatest at 110~112 days after planting (DAP) at the altitude of 800 m and 108~111 DAP at 600 m. There was no significant differences between altitudes and between cultivars. Tuber dry weight per plant at the altitude of 600 m was lower than 800 m on July 6 (58 DAP), but it increased rapidly from July 21 (73 DAP). At both altitudes, the increase of tuber dry weight per plant from July 6 to August 8 was higher than the other growth period. The time of growth period at which tuber dry weight per plant was the highest was similar at both altitudes that was 118~125 DAP at the altitude 800 m and 118~124 DAP at the altitude 600 m. Dry weight per tuber at the altitude 800m was higher than 600 m due to the number of tubers per plant. A higher increase of crop growth rate (CGR) was shown at the altitude 600 m on July 6 (58 DAP), comparing to at 800 m. The highest tuber dry weight per plant of each cultivar was shown when the planting time was April 29 for 'Superior' and was April 19 for 'Atlantic'. Both the tuber dry weight of plant and the total dry weight were lower at a later planting time. Dry weight per tuber increased quickly during the period between June 30 to August 8. Tuberization period was shortened as the planting time was delayed.

• Korean Journal of Medicinal Crop Science
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• v.7 no.3
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• pp.155-161
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• 1999

This study was carried out to develop the propagation system using in vitro induced- microtubers of yams (Dioscorea alata L.). Effects of kinds of media, mineral composition, sucrose concentration (0, 2, 4, 6, 8, 10%), photoperiod (0, 8, 12, 16, 24h), and growth regulators (NAA, IAA, ZR, JA-Me, ABA) on the development of microtubers, roots, and shoots in nodal stem segment cultures of D. alata L. were evaluated. Microtuberization in nodal stem segment occurred on all the media supplemented with growth regulator and sucrose. Among basic media, 1/2MS medium was the best in microtuber induction. NAA was shown to be the most effective among the growth regulators. Optimal NAA concentration was 1mg/l. The microtuberization was the highest at the concentration of 6% sucrose. When the nodal stem segment were cultured under darkness, the tuberization was increased markedly compared to those cultured under light condition. It was also noticeable that the culture in medium with NAA produced only microtubers and roots, but no shoots, in nodal segments. In this study, the optimal medium composition for microtuberization in nodal stem segment was found to be 1/2MS medium supplemented with 1mg/l NAA and 6% sucrose under dark condition at $25^{\circ}C$.

• Korean Journal of Weed Science
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• v.2 no.2
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• pp.114-121
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• 1982

Sagittaria pygmaea Miquel is widely infested and is the most dominant perennial weed in paddy field in Korea. This study was carried out in order to clarify the factors influencing tuber production and the competition ecology. The tuberization capacity in the paddy field transplanted with the small seedlings of rice was higher than in the paddy field transplanted with the vigor seedlings of rice. The stage which rice plants were most damaged by S. pygmaea plants was the period from 31 to 37 days after transplanting. According to the increasing of Eleocharis acicularis Roem. in the condition of rice cultivation, the number of S. pygmaea plants was remarkably reduced. Small size tubers planted within deep soil produced less tubers than large size tubers did. The number of plants and tubers were remarkably increased under the high levels of fertilization. Phosphorus was essential as well as nitrogen for tuberization. And the tuberization capapcity was different with the organic matter content in soil and with the soil moisture.

• Korean Journal of Weed Science
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• v.5 no.1
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• pp.43-49
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• 1985

This study was conducted to investigate the existance of dormancy in newly collected tubers of Eleocharis kuroguwai Ohwi from paddy fields and to determine the effect of various growth regulators on the bud sprouting and tuberization of E. kuroguwai. The maximum percent sprouting of E. kuroguwai collected at every month during winter was less than 60% regardless of varied collection tunes until 50 days after incubation, suggesting the presence of dormancy in E. kuroguwai tubers. This dormancy was markedly broken as the time went by, probably due to the exposure of tubers the extremly low temperature. The treatment of $BA10^{-3}M$ and $BA10^{-3}+GA10^{-6}M$ increased bud sprouting about 10 and 11 times, respectively, than that of the untreated control indicating that BA seems to be one of the most effective agents among the growth regulators used on the bud sprouting of E. kuroguwai. Foliar application of BA increased tuberization of E. kuroguwai by an average of 34.4% as compared with the untreated control. The highest increase, 39.8% was obtained with BA treatment at $10^{-5}M$.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1998.10a
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• pp.129-133
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• 1998

감자 무병종서의 대량급속 증식을 위해 기내소괴경 생산방법을 이용하는 연구가 많이 이루어져 왔다. 기내소괴경 형성에 관한 연구는 Grego(1956)에 의하여 최초로 가능성이 제시된 이후 많은 연구자들이 이에 대하여 보고하였으나 (Han, 1987; Hussey 등, 1984; Garcia 와 Gomez, 1973; Wiersema 등, 1987), 소괴경의 크기가 작아 파종후 입모율이 떨어지고, 생육초기 포장관리가 까다로운 점등의 문제점으로 실용화가 정착되지 못하고 있다. (중략)

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.157-157
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• 2003