• Maxillofacial Plastic and Reconstructive Surgery
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• v.39
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• pp.8.1-8.8
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• 2017

Background: For an effective bone graft for reconstruction of the maxillofacial region, an adequate vascular network will be required to supply blood, osteoprogenitor cells, and growth factors. We previously reported that the secretomes of bone marrow-derived mesenchymal stem cells (MSC-CM) contain numerous growth factors such as insulin-like growth factor (IGF)-1, transforming growth factor $(TGF)-{\beta}1$, and vascular endothelial growth factor (VEGF), which can affect the cellular characteristics and behavior of regenerating bone cells. We hypothesized that angiogenesis is an important step for bone regeneration, and VEGF is one of the crucial factors in MSC-CM that would enhance its osteogenic potential. In the present study, we focused on VEGF in MSC-CM and evaluated the angiogenic and osteogenic potentials of MSC-CM for bone regeneration. Methods: Cytokines in MSC-CM were measured by enzyme-linked immunosorbent assay (ELISA). Human umbilical vein endothelial cells (HUVECs) were cultured with MSC-CM or MSC-CM with anti-VEGF antibody (MSC-CM + anti-VEGF) for neutralization, and tube formation was evaluated. For the evaluation of bone and blood vessel formation with micro-computed tomography (micro-CT) and for the histological and immunohistochemical analyses, a rat calvarial bone defect model was used. Results: The concentrations of IGF-1, VEGF, and $TGF-{\beta}1$ in MSC-CM were $1515.6{\pm}211.8pg/mL$, $465.8{\pm}108.8pg/mL$, and $339.8{\pm}14.4pg/mL$, respectively. Tube formation of HUVECs, bone formation, and blood vessel formation were increased in the MSC-CM group but decreased in the MSC-CM + anti-VEGF group. Histological findings suggested that new bone formation in the entire defect was observed in the MSC-CM group although it was decreased in the MSC-CM + anti-VEGF group. Immunohistochemistry indicated that angiogenesis and migration of endogenous stem cells were much more abundant in the MSC-CM group than in the MSC-CM + anti-VEGF group. Conclusions: VEGF is considered a crucial factor in MSC-CM, and MSC-CM is proposed to be an adequate therapeutic agent for bone regeneration with angiogenesis.

• Journal of computational fluids engineering
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• v.16 no.1
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• pp.22-29
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• 2011

In this study, the glass fiber drawing from a silica preform in the furnace for the optical fiber manufacturing process is numerically simulated by considering the radiative heating of cylindrically shaped preform. The one-dimensional governing equations of the mass, momentum, and energy conservation for the heated and softened preform are solved as a set of the boundary value problems along with the radiative transfer approximation between the muffle tube and the deformed preform shape, while the furnace heating is modeled by prescribing the temperature distribution of muffle tube. The temperature-dependent viscosity of silica plays an important role in formation of preform neck-down profile when the glass fiber is drawn at high speed. The calculated neck-down profile of preform and the draw tension are found to be reasonable and comparable to the actual results observed in the optical fiber industry. This paper also presents the effects of key operating parameters such as the muffle tube temperature distribution and the fiber drawing speed on the preform neck-down profile and the draw tension. Draw tension varies drastically even with the small change of furnace heating conditions such as maximum heating temperature and heating width, and the fine adjustment of furnace heating is required in order to maintain the appropriate draw tension of 100~200 g.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.639-639
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• 2013

In this research we report the significant contribution of the as-spun multi-walled carbon nanotube (MWCNT) on the x-ray images formation using a low tube voltage x-ray source. The MWCNT, which was used for the fabrication of the spun CNT, was grown using a microwave plasma-enhanced chemical vapor deposition machine. Electrical-optics simulation software was utilized to determine the electron field emission trajectory of the triode-structure-as-spun CNT-based x-ray source. It was shown that a significant amount of converging electrons hit the target anode producing a clear x-ray image. These x-ray images where produced at a small amount of anode current of 0.67 mA at a tube voltage of 5 kV with the gate voltage of 0 V. Also, comparisons of the radiographs at various exposure times of the sample where analyzed with and without an x-ray dose filter. Results showed that spatially-resolved images were formed using the as-spun CNT at a low tube voltage with a $54-{\mu}m$ Al x-ray filter. This study can be used for low-voltage medical applications.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.10
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• pp.1686-1694
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• 2003

The foam-filled tube beams can be used for the front rail and firewall structures to absorb impact energy during frontal or side collision of vehicles. In the case of side collision where bending is involved in the crushing mechanism, the foam filler would be effective in maintaining progressive crushing of the thin-walled structures so that much impact energy could be absorbed. In this study, bending behaviors of the closed-cell-aluminum-alloy-foam-filled stainless steel tube were investigated. The various foam-filled specimens including piecewise fillers were prepared and tested. The aluminum-alloy-foam filling offered the significant increase of bending resistance. Their suppression of the inward fold formation at the compression flange as well as the multiple propagating folds led to the increase of load carrying capacity of specimens. Moreover, the piecewise foams would provide the easier way to fill the thin-walled shell structures without the drawback of strength.

• Nuclear Engineering and Technology
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• v.47 no.4
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• pp.454-460
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• 2015

Acoustic emission (AE) is one of the promising methods for detecting the formation of stress corrosion cracks (SCCs) in laboratory tests. This method has the advantage of online inspection. Some studies have been conducted to investigate the characteristics of AE parameters during SCC propagation. However, it is difficult to classify the distinct features of SCC behavior. Because the previous studies were performed on slow strain rate test or compact tension specimens, it is difficult to make certain correlations between AE signals and actual SCC behavior in real tube-type specimens. In this study, the specimen was a AISI 304 stainless steel tube widely applied in the nuclear industry, and an accelerated test was conducted at high temperature and pressure with a corrosive environmental condition. The study result indicated that intense AE signals were mainly detected in the elastic deformation region, and a good correlation was observed between AE activity and crack growth. By contrast, the behavior of accumulated counts was divided into four regions. According to the waveform analysis, a specific waveform pattern was observed during SCC development. It is suggested that AE can be used to detect and monitor SCC initiation and propagation in actual tubes.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.1133-1138
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• 2003

The pressure tubes, which contain high temperature heavy water and fuel, are within the core of a CANDU nuclear reactor, and are thus subjected to high stresses, temperature gradient, and neutron flux. Further, it is well known that pressure tubes of cold-worked Zr-2.5Nb materials result in hydrogen diffusion, which create fully-hydrided regions (frequently called Blister). Thus a proper investigation of hydrogen diffusion within zirconium-alloy nuclear components, such as CANDU pressure tube and fuel channels is essential to predict the structural integrity of these components. In this respect, this paper presents numerical investigation of hydrogen diffusion to quantify the hydrogen concentration for blister growth of CANDU pressure tube. For this purpose, coupled temperature-hydrogen diffusion analyses are performed by means of two-dimensional finite element analysis. Comparison of predicted temperature field and blister with published test data shows good agreement.

• Journal of the Korean Society for Precision Engineering
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• v.28 no.10
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• pp.1210-1216
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• 2011

The purpose of this study is to establish the analysis method for prediction of temperature during cryogenic heat treatment. Experimental cryogenic heat treatment is conducted to observe the phenomena such as boiling of fluid, ice layer on the material surface and to measure the temperature distribution of Al6061 tube. The CFD analysis considering the observed phenomena in the experiment is performed to predict the temperature distribution and convection heat transfer coefficient at each stage of cryogenic heat treatment, in which the boiling of fluid is considered as the multi-phase condition of vapour and liquid. The formation of ice layer on the tube surface is also modeled between material and fluid. The predicted results are in good agreement with the experimental ones. From the results, it is shown that the analysis method can predict the temperature distribution and convection heat transfer coefficient during cryogenic heat treatment.

• Journal of ILASS-Korea
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• v.19 no.2
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• pp.75-81
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• 2014

Swirl flow in the gun type burner has a decisive effect on the stabilization of the flame, improvement of the combustion efficiency, and also a reduction of NOx. This swirl flow is created by the spinner which is inside the airtube that guide the combustion air. Gun type burner has generally the inner devices composed nozzle adapter, spark gap ignitor, and spinner. These inner components change the air flow behavior passing through air tube. Meanwhile, turbulent characteristics of this air flow are important to understand the combustion phenomena in the gun type burner, because the mixture of fuel and air are depended on. However, nearly all of the studies have been analyzed the turbulent flow of simplified combustion formation without the inner devices. So, this study conducted the measurement using by hot-wire anemometer and analyzed turbulent flow characteristics of the swirl flow discharged from the air tube with inner devices. Turbulence characteristics come up in this study were turbulence intensity, kinetic energy and shear stress of the air flow with the change of the distance of axial direction from the exit of the air tube.

• Korean Journal of Microbiology
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• v.16 no.3
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• pp.111-121
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• 1978

About 40 temperature-sensitive mutants have been isolated as a preliminary step to study the spore germination, the cell cycle, and the control of macromolecular synthesis in Aspergillus nidulans. To obtain temperature-sensitive mutants rapidly and effectively, the selective enrichment method using antifungal antibiotic nystatin was developed. Based on the data which had applied to the concentration of auxotrophic mutants by the earlier investigators, the optimal concentration and the time of treatment at the nonpermissive temeprature were determined as 50 to 100 units per ml and 4.5 hr., respectively. Out of 41 ts mutants assigned to the strain symbol PK, thirteen that seemed to be arrested at the earlystage of spore germination were subjected to the further cytological and genetic analysis. Elght of these mutants are able to form germ tube and five not. Staining with acid fuchsin for the 5PK strains shows that one irreversible mutant, PK6 strain able to form germ tube, accumulate mitotic spindle, being arrested in mitosis. Another PK15 and PK23 strain have more than one intact nucleolus without germ tube formation at the restrictive temperature. the temperature-senstive mutation in PK12 strain, the onlystrain which is able occurred in certain gene specific for the germination of spore. All of the ts markers are recessive and complement each other in heterokaryon between two different ts markers at the restrictive temperature.

• International Journal of Air-Conditioning and Refrigeration
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• v.11 no.1
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• pp.1-9
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• 2003

The examinations of the operating mechanism of an oscillating capillary tube heat pipe (OCHP) using the visualization method revealed that the working fluid in the OCHP oscillated to the axial direction by the contraction and expansion of vapor plugs. The contraction and expansion were due to the formation and extinction of bubbles in the evaporating and condensing part, respectively The actual physical mechanism, whereby the heat which was transferred in such an OCHP was complex and not well understood. In this study, a theoretical model of the OCHP was developed to model the oscillating motion of working fluid in the OCHP. The differential equations of two-phase flow were applied and simultaneous non-linear partial differential equations were solved. From the analysis of the numerical results, it was found that the oscillating motion Of working fluid in the OCHP was affected by the operation and design conditions such as the heat flux, the charging ratio of working fluid and the hydraulic diameter of flow channel. The simulation results showed that the proposed model and solution could be used for estimating the operating mechanism in the OCHP.