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Characterization and Enhancement of Package O2 Barrier against Oxidative Deterioration of Powdered Infant Formula

  • Jo, Min Gyeong;An, Duck Soon;Lee, Dong Sun
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.24 no.1
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    • pp.13-16
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    • 2018
  • Powdered infant formula is susceptible to oxidation in the presence of oxygen. Even though the product is usually packaged in nitrogen atmosphere, the oxygen ingress through the package layer may occur in case of flexible pouches and affects the oxidation of the product. $O_2$ barrier of the package is thus important variable to protect the product from oxidative deterioration. $O_2$ barrier property was investigated for aluminum-laminated small pillow packs of $3.5{\times}17.5cm$. Storage temperature and combination of primary and secondary packages were evaluated as variables affecting the barrier for conditions of empty pouch flushed with nitrogen. Apparent oxygen transmission rate of the primary package exposed to air was $2.32{\times}10^{-3}mL\;(STP)\;atm^{-1}\;d^{-1}$ at $30^{\circ}C$ and its temperature dependence could be explained by activation energy of $28.5kJ\;mol^{-1}$ in Arrhenius relationship. The additional secondary package of nylon/PE film containing 20 primary packages was ineffective in modulating package $O_2$ transmission and was only marginally helpful when combined with oxygen scavenger. The same was true in suppressing the product oxidation when the primary package was filled with 14 g of the formula.

Detection of Human Adenoviruses and Enteroviruses in Korean Oysters Using Cell Culture, Integrated Cell Culture-PCR, and Direct PCR

  • Choo Yoe-Jin;Kim Sang-Jong
    • Journal of Microbiology
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    • v.44 no.2
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    • pp.162-170
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    • 2006
  • Oysters are known to be carriers of food-born diseases, but research on viruses in Korean oysters is scarce despite its importance for public health. We therefore tested oysters cultivated in Goheung, Seosan, Chungmu, and Tongyeong, for viral contamination using cell culture and integrated cell culture PCR (ICC-PCR) with Buffalo green monkey kidney (BGMK) and human lung epithelial (A549) cells. Additional screens via PCR, amplifying viral nucleic acids extracted from oysters supplemented our analysis. Our methods found 23.6 %, 50.9 %, and 89.1 % of all oysters to be positive for adenoviruses when cell culture, ICC-PCR, and direct PCR, respectively, was used to conduct the screen. The same methodology identified enteroviruses in 5.45%, 30.9%, and 10.9% of all cases. Most of the detected enteroviruses (81.3%) were similar to poliovirus type 1; the remainder resembled coxsackievirus type A1. A homology search with the adenoviral sequences revealed similarities to adenovirus subgenera C (type 2, 5, and 6), D (type 44), and F (enteric type 40 and 41). Adenovirus-positive samples were more abundant in A549 cells (47.3%) than in BGMK cells (18.2 %), while the reverse was true for enteroviruses (21.8 % vs. 14.5 %). Our data demonstrate that Korean oysters are heavily contaminated with enteric viruses, which is readily detectable via ICC-PCR using a combination of A549 and BGMK cells.

Heterologous Expression of a Putative $K^+/H^+$ Antiporter of S. coelicolor A3(2) Enhances $K^+$, Acidic-pH Shock Tolerances, and Geldanamycin Secretion

  • Song, Jae Yang;Seo, Young Bin;Hong, Soon-Kwang;Chang, Yong Keun
    • Journal of Microbiology and Biotechnology
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    • v.23 no.2
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    • pp.149-155
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    • 2013
  • Heterologous expression of a putative $K^+/H^+$ antiporter of Streptomyces coelicolor A3(2) (designated as sha4) in E. coli and Streptomyces hygroscopicus JCM4427 showed enhanced tolerance to $K^+$ stress, acidic-pH shock, and/or geldanamycin production under $K^+$ stress. In a series of $K^+$ extrusion experiments with sha4-carrying E. coli deficient in the $K^+/H^+$ antiporter, a restoration of impaired $K^+$ extrusion activity was observed. Based on this, it was concluded that sha4 was a true $K^+/H^+$ antiporter. In different sets of experiments, the sha4-carrying E. coli showed significantly improved tolerances to $K^+$ stresses and acidic-pH shock, whereas sha4-carrying S. hygroscopicus showed an improvement in $K^+$ stress tolerance only. The sha4-carrying S. hygroscopicus showed much higher geldanamycin productivity than the control under $K^+$ stress condition. In another set of experiments with a production medium, the secretion of geldanamycin was also significantly enhanced by the expression of sha4.

Alignment between National College Entrance Examinations and Mathematics Curriculum Standards: A Comparative Analysis

  • Hong, Dae S.;Bae, Yejun;Wu, Yu-Fen
    • Research in Mathematical Education
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    • v.22 no.3
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    • pp.153-174
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    • 2019
  • Measuring alignment of various educational components is an important issue in educational research because with aligned educational system, we can have clear expectations about what to teach and assess. In this study, we examined the alignment between mathematics curriculum standards and college entrance examinations from Korea and China. The results indicate that curriculum standards and high stakes assessments from both countries are not well aligned to each other. Their Surveys of Enacted Curriculum (SEC) indices were lower than what previous studies have found and the critical values (Fulmer, 2011; Liu & Fulmer, 2008; Liu et al., 2009). There are several topics that are not assessed in both countries' national assessments. Also, discrepancies between the most frequently covered topics in the curriculum standards and the most frequently assessed mathematical topics in the national assessments caused topic level misalignment. We also found misalignment in cognitive level. Both national assessments included more perform procedures and demonstrate understanding items than their respective curriculum standards. Thus, previous findings about the inclusion of more items with higher cognitive demand in assessments is only partially true for either country. With these results, it is difficult to say that whether mathematical topics in the curriculum standards appropriately represent and support students to do well on the CSAT and the NCEE or that the mathematical items in the CSAT and the NCEE validly assess students' level of mathematical understanding.

Attention-LSTM based Lane Change Possibility Decision Algorithm for Urban Autonomous Driving (도심 자율주행을 위한 어텐션-장단기 기억 신경망 기반 차선 변경 가능성 판단 알고리즘 개발)

  • Lee, Heeseong;Yi, Kyongsu
    • Journal of Auto-vehicle Safety Association
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    • v.14 no.3
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    • pp.65-70
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    • 2022
  • Lane change in urban environments is a challenge for both human-driving and automated driving due to their complexity and non-linearity. With the recent development of deep-learning, the use of the RNN network, which uses time series data, has become the mainstream in this field. Many researches using RNN show high accuracy in highway environments, but still do not for urban environments where the surrounding situation is complex and rapidly changing. Therefore, this paper proposes a lane change possibility decision network by adopting Attention layer, which is an SOTA in the field of seq2seq. By weighting each time step within a given time horizon, the context of the road situation is more human-like. A total 7D vectors of x, y distances and longitudinal relative speed of side front and rear vehicles, and longitudinal speed of ego vehicle were used as input. A total 5,614 expert data of 4,098 yield cases and 1,516 non-yield cases were used for training, and the performance of this network was tested through 1,817 data. Our network achieves 99.641% of test accuracy, which is about 4% higher than a network using only LSTM in an urban environment. Furthermore, it shows robust behavior to false-positive or true-negative objects.

Three-Dimensional Image Reconstruction from Compton Scattered Data Using the Row-Action Maximum Likelihood Algorithm (행작용 최대우도 알고리즘을 사용한 컴프턴 산란 데이터로부터의 3차원 영상재구성)

  • Lee, Mi-No;Lee, Soo-Jin;Nguyen, Van-Giang;Kim, Soo-Mee;Lee, Jae-Sung
    • Journal of Biomedical Engineering Research
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    • v.30 no.1
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    • pp.56-65
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    • 2009
  • Compton imaging is often recognized as a potentially more valuable 3-D technique in nuclear medicine than conventional emission tomography. Due to inherent computational limitations, however, it has been of a difficult problem to reconstruct images with good accuracy. In this work we show that the row-action maximum likelihood algorithm (RAMLA), which have proven useful for conventional tomographic reconstruction, can also be applied to the problem of 3-D reconstruction of cone-beam projections from Compton scattered data. The major advantage of RAMLA is that it converges to a true maximum likelihood solution at an order of magnitude faster than the standard expectation maximiation (EM) algorithm. For our simulations, we first model a Compton camera system consisting of the three pairs of scatterer and absorber detectors placed at x-, y- and z-axes, and generate conical projection data using a software phantom. We then compare the quantitative performance of RAMLA and EM reconstructions in terms of the percentage error. The net conclusion based on our experimental results is that the RAMLA applied to Compton camera reconstruction significantly outperforms the EM algorithm in convergence rate; while computational costs of one iteration of RAMLA and EM are about the same, one iteration of RAMLA performs as well as 128 iterations of EM.

Interspecific protoplast Fusion and Sexuality in Pleurotus (느타리의 종간(種間) 원형질체(原形質體) 융합(融合) 및 유성(有性))

  • Yoo, Young-Bok;You, Chang-Hyun;Park, Yong-Hwan;Lee, Yeon-Hee;Chang, Kwon-Yawl;Peberdy, John F.
    • The Korean Journal of Mycology
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    • v.15 no.3
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    • pp.135-141
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    • 1987
  • Interspecific heterokaryons were obtained by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of P. ostreatus+P. cornucopiae, P. ostreatus+P. florida, P. ostreatus+P. sajor-caju, P. florida+P. cornucopiae, P. florida+P. sajor-caju and P. sajor-caju+P. cornucopiae protoplasts. The Fusion products of protoplasts were induced by MCM+benomyl, but segregation of sectors were not identified. Interspecific fusion products of protoplasts between incompatible strains did not form true clamp connections and did not produce fruit bodies. For induction of fertility, interspecific heterokaryons crossed with their parents by hyphal anastomosis.

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Development of Efficient Monitoring Algorithm at EGS Site by Using Microseismic Data (미소진동 자료를 이용한 EGS 사이트에서의 효율적인 모니터링 알고리듬 개발)

  • Lee, Sangmin;Byun, Joongmoo
    • Geophysics and Geophysical Exploration
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    • v.19 no.3
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    • pp.111-120
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    • 2016
  • In order to enhance the connectivity of fracture network as fluid path in enhanced/engineered geothermal system (EGS), the exact locating of hydraulic fractured zone is very important. Hydraulic fractures can be tracked by locating of microseismic events which are occurred during hydraulic fracture stimulation at each stage. However, since the subsurface velocity is changed due to hydraulic fracturing at each stage, in order to find out the exact location of microseismic events, we have to consider the velocity change due to hydraulic fracturing at previous stage when we perform the mapping of microseimic events at the next stage. In this study, we have modified 3D locating algorithm of microseismic data which was developed by Kim et al. (2015) and have developed 3D velocity update algorithm using occurred microseismic data. Eikonal equation which can efficiently calculate traveltime for complex velocity model at anywhere without shadow zone is used as forward engine in our inversion. Computational cost is dramatically reduced by using Fresnel volume approach to construct Jacobian matrix in velocity inversion. Through the numerical test which simulates the geothermal survey geometry, we demonstrated that the initial velocity model was updated by using microseismic data. In addition, we confirmed that relocation results of microseismic events by using updated velocity model became closer to true locations.

A Study on a Calculation Method of Economical Intake Water Depth in the Design of Head Works (취입모의 경제적 계획취입수심 산정방법에 대한 연구)

  • 김철기
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.20 no.1
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    • pp.4592-4598
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    • 1978
  • The purpose of this research is to find out mathemetically an economical intake water depth in the design of head works through the derivation of some formulas. For the performance of the purpose the following formulas were found out for the design intake water depth in each flow type of intake sluice, such as overflow type and orifice type. (1) The conditional equations of !he economical intake water depth in .case that weir body is placed on permeable soil layer ; (a) in the overflow type of intake sluice, {{{{ { zp}_{1 } { Lh}_{1 }+ { 1} over {2 } { Cp}_{3 }L(0.67 SQRT { q} -0.61) { ( { d}_{0 }+ { h}_{1 }+ { h}_{0 } )}^{- { 1} over {2 } }- { { { 3Q}_{1 } { p}_{5 } { h}_{1 } }^{- { 5} over {2 } } } over { { 2m}_{1 }(1-s) SQRT { 2gs} }+[ LEFT { b+ { 4C TIMES { 0.61}^{2 } } over {3(r-1) }+z( { d}_{0 }+ { h}_{0 } ) RIGHT } { p}_{1 }L+(1+ SQRT { 1+ { z}^{2 } } ) { p}_{2 }L+ { dcp}_{3 }L+ { nkp}_{5 }+( { 2z}_{0 }+m )(1-s) { L}_{d } { p}_{7 } ] =0}}}} (b) in the orifice type of intake sluice, {{{{ { zp}_{1 } { Lh}_{1 }+ { 1} over {2 } C { p}_{3 }L(0.67 SQRT { q} -0.61)}}}} {{{{ { ({d }_{0 }+ { h}_{1 }+ { h}_{0 } )}^{ - { 1} over {2 } }- { { 3Q}_{1 } { p}_{ 6} { { h}_{1 } }^{- { 5} over {2 } } } over { { 2m}_{ 2}m' SQRT { 2gs} }+[ LEFT { b+ { 4C TIMES { 0.61}^{2 } } over {3(r-1) }+z( { d}_{0 }+ { h}_{0 } ) RIGHT } { p}_{1 }L }}}} {{{{+(1+ SQRT { 1+ { z}^{2 } } ) { p}_{2 } L+dC { p}_{4 }L+(2 { z}_{0 }+m )(1-s) { L}_{d } { p}_{7 }]=0 }}}} where, z=outer slope of weir body (value of cotangent), h1=intake water depth (m), L=total length of weir (m), C=Bligh's creep ratio, q=flood discharge overflowing weir crest per unit length of weir (m3/sec/m), d0=average height to intake sill elevation in weir (m), h0=freeboard of weir (m), Q1=design irrigation requirements (m3/sec), m1=coefficient of head loss (0.9∼0.95) s=(h1-h2)/h1, h2=flow water depth outside intake sluice gate (m), b=width of weir crest (m), r=specific weight of weir materials, d=depth of cutting along seepage length under the weir (m), n=number of side contraction, k=coefficient of side contraction loss (0.02∼0.04), m2=coefficient of discharge (0.7∼0.9) m'=h0/h1, h0=open height of gate (m), p1 and p4=unit price of weir body and of excavation of weir site, respectively (won/㎥), p2 and p3=unit price of construction form and of revetment for protection of downstream riverbed, respectively (won/㎡), p5 and p6=average cost per unit width of intake sluice including cost of intake canal having the same one as width of the sluice in case of overflow type and orifice type respectively (won/m), zo : inner slope of section area in intake canal from its beginning point to its changing point to ordinary flow section, m: coefficient concerning the mean width of intak canal site,a : freeboard of intake canal. (2) The conditional equations of the economical intake water depth in case that weir body is built on the foundation of rock bed ; (a) in the overflow type of intake sluice, {{{{ { zp}_{1 } { Lh}_{1 }- { { { 3Q}_{1 } { p}_{5 } { h}_{1 } }^{- {5 } over {2 } } } over { { 2m}_{1 }(1-s) SQRT { 2gs} }+[ LEFT { b+z( { d}_{0 }+ { h}_{0 } )RIGHT } { p}_{1 }L+(1+ SQRT { 1+ { z}^{2 } } ) { p}_{2 }L+ { nkp}_{5 }}}}} {{{{+( { 2z}_{0 }+m )(1-s) { L}_{d } { p}_{7 } ]=0 }}}} (b) in the orifice type of intake sluice, {{{{ { zp}_{1 } { Lh}_{1 }- { { { 3Q}_{1 } { p}_{6 } { h}_{1 } }^{- {5 } over {2 } } } over { { 2m}_{2 }m' SQRT { 2gs} }+[ LEFT { b+z( { d}_{0 }+ { h}_{0 } )RIGHT } { p}_{1 }L+(1+ SQRT { 1+ { z}^{2 } } ) { p}_{2 }L}}}} {{{{+( { 2z}_{0 }+m )(1-s) { L}_{d } { p}_{7 } ]=0}}}} The construction cost of weir cut-off and revetment on outside slope of leeve, and the damages suffered from inundation in upstream area were not included in the process of deriving the above conditional equations, but it is true that magnitude of intake water depth influences somewhat on the cost and damages. Therefore, in applying the above equations the fact that should not be over looked is that the design value of intake water depth to be adopted should not be more largely determined than the value of h1 satisfying the above formulas.

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An Application of loop-loop EM Method for Geotechnical Survey (지반조사를 위한 loop-loop 전자탐사 기법의 적용)

  • You Jin-Sang;Song Yoonho;Seo1 Soon-Jee;Song Young-Soo
    • Geophysics and Geophysical Exploration
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    • v.4 no.2
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    • pp.25-33
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    • 2001
  • Loop-loop electromagnetic (EM) survey in frequency domain has been carried out in order to provide basic solution to geotechnical applications. Source and receiver configuration may be horizontal co-planar (HCP) and/or vertical co-planar (VCP). Three quadrature components of mutual impedance ratio for each configuration are used to construct the subsurface image. For the purpose of obtaining the model response and validating the reasonable performance of the inversion, we obtained each responses of two-layered and three-layered earth models and two-dimensional (2-D) isolated anomalous body. The response of 2-D isolated anomalous body has been calculated using extended Born approximation for the solution of 2.5-D integral equation describing EM scattering problem. As a result of the least-squares inversion with variable Lagrangian multiplier, we could construct more resolvable image from HCP data than VCP data. Furthermore, joint inversion of HCP and VCP data made better stability and resolution of the inversion. Resistivity values, however, did not exactly match the true ones. Loop-loop EM field data was obtained with EM34-3XL system manufactured by Geonics Ltd. (Canada). Electrical resistivity survey was conducted on the same line for the comparison in advance. Since the constructed image from loop-loop EM data by 2-D inversion algorithm showed almost similar resistivity distribution to that from electrical resistivity one, we expect the developed 2.5-D loop-loop EM inversion program can be applied for the reconnaissance site survey.

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