• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.253-253
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• 2022

Codonopsis lanceolata (C. lanceolata) has been widely used in East Asia as a traditional medicine to treat various diseases such as bronchitis, convulsions, cough, obesity, and hepatitis. C. lanceolata belonging to Campanulaceae contains bioactive compounds such as polyphenols, saponins, and steroids. However, despite the pharmacological significance of C. lanceolata, the genetic information of this plant is limited and there are few studies of its transcriptome. In this study, we constructed a unigene set of C. lanceolata using Pac-Bio sequencing. Furthermore, the reads generated from Pac-bio and Illumina sequencing were mixed and assembled using rnaSPAdes. All genes involved in the triterpenoid pathway, a major bioactive compounds of C. lanceolata, were searched from the two unigene sets and the expression profiles of these genes were analyzed. The results showed that lupeol, beta-amyrin, and dammarenediol synthesis genes were activated in the leaves and roots of C. lanceolata. In particular, the expression of genes related to lupeol synthesis was relatively high, suggesting that the main triterpenoid of C. lanceolata is lupeol. Transcriptome studies related to lupeol synthesis in C. lanceolata have been rarely reported. Lupeol has been reported to have pharmacological effects such as anti-inflammatory, anti-cancer, and anti-bacterial. This study suggests the importance of C. lanceolata as a lupeol producing plant.

• YAKHAK HOEJI
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• v.19 no.3
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• pp.159-163
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• 1975

• Natural Product Sciences
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• v.6 no.2
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• pp.66-69
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• 2000

Salvia bucharica belonging to the family Lamiaceae (Labiatae), afforded a novel terpenoid (1) named as salvatrione. The structure of 1 was elucidated through extensive 2-D NMR experiments and the biogenetic pathway of 1 has also been proposed. On biogenetic grounds, 1 may be considered to be a pseudo-triterpenoid as it is derived from the coupling of mono/ diterpenic units and not from squalene.

• 한국약용작물학회:학술대회논문집
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• 2011.09a
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• pp.334-335
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• 2011

• Natural Product Sciences
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• v.15 no.3
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• pp.173-179
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• 2009

To determine the cytotoxic activity of natural compounds, chromatographic separation of the hexanesoluble fraction from the fruiting body of Ganoderma lucidum led to the isolation of four steroids and one triterpenoid. They were identified as ergosterol peroxide (1), stella sterol (2), ergosterol (3), 9(11)-dehydroergostrol peroxide (4), and ganodermanontriol (5) based on spectroscopic evidence and physicochemical properties. These compounds were examined for their cytotoxic activity against HL-60, MCF-7, and LLC cancer cell lines. Ganodermanontriol (5) showed cytotoxic activity with IC$_{50}$ values of 24.8 and 22.9 $\mu$g/mL against HL-60 and MCF-7 cancer cell lines, respectively, whereas compounds 1 - 4 were inactive.

• Archives of Pharmacal Research
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• v.27 no.8
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• pp.825-828
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• 2004

Two triterpenoids (1,4) and two triterpenoid glycosides (2,3) were isolated from the root of Acanthopanax koreanum (Araliaceae). Their structures were identified as impressic acid (1), acankoreoside A (2), 3-epi-betulinic acid 28-O-［(${\alpha}-L-rhamnopyranosyl(1{\rightarrow}4)-{\beta}-D-glucopyrano-syl(1{\rightarrow}6)]-{\beta}-D-glucopyranosyl]$ ester (3), and ursolic acid (4) by physicochemical and spectro-scopic methods. Of these compounds, impressic acid (1) exhibited a potent inhibitory activity against NFAT transcription factor ($IC_{50}:{\;}12.65{\;}{\mu\textrm{m}}$).

• Biomedical Science Letters
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• v.19 no.3
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• pp.224-232
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• 2013

Cucurbitacins are a natural cell-permeable triterpenoid compound isolated from Cucurbitaceae and Cruciferae. Cucurbitacins have been used as folk medicine because of their anti-inflammatory and analgesic effects. In the present study, we investigate the anti-cancer effects of cucurbitacin I on colitis-associated colon carcinogenesis induced by azoxymethane (AOM)/dextran sodium sulfate (DSS) in BALB/c mice. Cucurbitacin I treatment attenuated loss of body weight and decreased the number of colon tumors. Western blot analysis showed that cucurbitacin I treatment significantly inhibited the protein expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-6. These results suggest that cucurbitacin I suppressed inflammatory reaction and tumor development in colitis-associated colon carcinogenesis.

• Korean Journal of Pharmacognosy
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• v.38 no.1
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• pp.76-83
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• 2007

In this study, three triterpenoids, two steroids and nine flavonoids were isolated from the leaves of Alnus firma Sieb. et Zucc. On the basis of spectroscopic evidences, the structures of these compounds were established as ${\beta}$-amyrin acetate, ${\beta}$-amyrin, ${\beta}$-sitosterol, alnustic acid methyl ester, ${\beta}$-sitosterol glucoside, pinocembrin, alnustinol, quercetin, quercetin-3-O-${\alpha}$-L-arabinofuranoside, quercetin-3-O-${\alpha}$-L -rhamnopyranoside, quercetin-3-O-${\beta}$-D-glucopyranoside, myricetin-3-O-${\beta}$-D-galac-topyranoside, (+)-catechin and (-)-epicatechin.

• Natural Product Sciences
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• v.20 no.3
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• pp.146-151
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• 2014

The purification of the MeOH extracts from the aerial parts of Aceriphyllum rossii Engler (Saxifragaceae) using column chromatography furnished fourteen compounds (1 - 14). The chemical structures of the isolated compounds were determined using mainly nuclear magnetic resonance spectra and mass spectrometry. All the isolated compounds were tested for their cytotoxic activity against LH-60, MCF-7 and HeLa cancer cells in vitro using a MTT assay method. Compounds possessing an olean-triterpenoid skeleton entirely exhibit dose dependent cytotoxic activity. These findings partially confirmed the anticancer effect of this medicinal plant, suggesting a further study on the anticancer potential of the triterpenoid structure in compounds from this plant.

• Natural Product Sciences
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• v.12 no.4
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• pp.193-196
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• 2006

A mixture of cerebrosides (1) and four triterpenoids (2 - 5) have been isolated from the hexane- and EtOAc-soluble fractions of the roots of Synurus deltoides (Ait.) Nakai (Compositae). Triterpenoid structures were determined as lupeol (2), $\beta-amyrin$ (3), $\alpha-amyrin$ (4), and ursolic acid (5). Synurus cerebrosides (1) were characterized as a common long chain base (2S,3S,4R,8E)-2-amino-8-octadecene-1,3,4-triol and fatty acyl chains; palmitic acid, (2R)-2-hydroxybehenic acid, (2R)-2-hydroxytricosanoic acid, (2R)-2-hydroxylignoceric acid, (2R)-2-hydroxypentacosanoic acid, and (2R)-2-hydroxyhexacosanoic acid. The synurus cerebrosides (1) were the first isolation from a natural source.