• 한국동물위생학회지
• /
• 제15권2호
• /
• pp.101-108
• /
• 1992

This study was carried out to investigate the effect of sulfamethazine and trimethoprim on TTC test in raw milk. Ten raw milks which does not contain any antibiotics were collected from milk farms and one hundred twenty raw milks which collected from three raw milk receiving stations and those milk samples were mixed up sulfamethazine and trimethoprim for TTC test. The results were as follows ; 1. Positive reactions of TTC test were shown above the concentration of 3, 000ppm for sulfamethazine and 10ppm for trimethoprim. 2. When the TTC test was conducted with trimethoprim and sulfamethazine mixture, a pair of the concentration of trimethoprim and sulfamethazine shown to positive reaction were 0.1-2, 000ppm, 0.5-1, 000ppm, 1.0-250ppm and 2.0-100ppm, respectively. 3. Of 120 raw milk samples tested, 16 samples(13%) were shown quasi-positive reaction when the trimethoprim at the concetration of 2ppm was added in the samples.

• 한국수산과학회지
• /
• 제41권5호
• /
• pp.324-329
• /
• 2008

Vibrio parahaemolyticus species, which cause acute gastroenteritis in humans, were isolated from farmed fish and seawater and their antimicrobial-resistance pattern and factor were investigated. They exhibited the highest resistance to ampicillin (88.9%), followed by trimethoprim (51.9%) and rifampin (22.2%). The relatively high resistance to trimethoprim was unexpected because trimethoprim was not commonly used in fish farming in Korea. R plasmid related resistance was identified by the treatment of novobiocin (7 ug/mL) and it was named as pVPBW1. A putative trimethoprim resistance gene in 2.0 kb fragment of pVPBW1 was also confirmed.

• 한국수산과학회지
• /
• 제43권2호
• /
• pp.125-130
• /
• 2010

A trimethoprim resistant Vibrio parahaemolyticus, which cause acute gastroenteritis in humans, was isolated from farmed fish and seawater. The resistance profiles of isolated V. parahaemolyticus and their correlation with mobile elements were investigated. All of the V. parahaemolyticus were resistance to both rifampin and trimethoprim. The presence of class I integron was confirmed by PCR. PCR-amplified inserted gene cassettes contained aminoglycoside aac6-II, rifampin arr-3 and trimethoprim dfrA27 resistance genes. This study indicated that class I integron mainly contributed to the circulation of trimethoprim resistance determinants in V. parahaemolyticus.

• 분석과학
• /
• 제36권5호
• /
• pp.250-257
• /
• 2023

The development of a spectrofluorimetric method for the determination of trimethoprim according to the reaction between O-phthalaldehyde (OPA) in highly alkaline media, and 2-mercaptoethanol (2ME) and this reaction gives fluorescent product measured at (458) nm when excited at (342) nm. The optimization of the analytical parameters that influence intensity was investigated. The intensity of fluorescence of the formed product was linearly related to the concentration of trimethoprim in the (100-1200) ng mL^-1 range. The limit of detection and limit of quantification were estimated to be (22.54) ng mL^-1 and (75.15) ng mL^-1 respectively. The utility of the proposed methods was successfully verified by analysis of trimethoprim in pure and real pharmaceutical preparations with high accuracy, the recovery percentages Re%, were found to be (100.5) % and (99.76) % for pure drug and pharmaceutical preparations respectively.

• 한국임상수의학회지
• /
• 제25권2호
• /
• pp.115-118
• /
• 2008

22 dogs (31 eyes) that had treated with trimethoprim-sulfamethoxazole for tear staining syndrome at Snoopy Pet Clinic from October 2000 to September 2002 were reviewed. Of the 22 dogs, 12 were female and 10 male. Their mean (${\pm}$ SD) age was 3.5 (${\pm}\;1.3$) years. The breeds of the dogs consisted of Maltese (8 dogs), Shih tzu (6 dogs), Poodle (5 dogs), Yorkshire terrier (2 dogs), and Mixed (1 dog). The dogs received 30 mg/kg trimethoprim-sulfamethoxazole perorally twice daily for two to six weeks. 26 (19 dogs) of the 31 eyes (22 dogs) recovered completely and did not show relapse at $26{\sim}30$ weeks after treatment. Any complications did not observed. Five eyes of three dogs were not cured. Two eyes (one dogs) of them had not response to medicament and three eyes (two dogs) recurrence but the clinical signs decreased. It was considered that the trimethoprim-sulfamethoxazole was effective for the treatment in dogs with tear staining syndrome.

• Asian-Australasian Journal of Animal Sciences
• /
• 제4권3호
• /
• pp.281-284
• /
• 1991

Combination effects of sulfamethoxazole (SMX) and trimethoprim (TMP) against nine gram positive bacterial strains and 43 gram negative bacterial strains which included 40 strains of animal intestinal bacteria were studied in vitro. Minimum inhibitory concentrations (MICs) of SMX and TMP alone and 20:1 (SMX : TMP) mixture (ST) were investigated by the method recommended by Ad Hoc Committee of the Japan Society of Chemotherapy for the Evaluation of Sensitivity Testing Methods for Sulfamethoxazole and Trimethoprim. MICs of ST were more potentiated than those of SMX alone in 8 of 9 gram positive strains and 40 of 43 gram negative strains. Especially, 38 strains of 40 intestinal bacteria showed significant susceptibility to ST as compared to SMX. These results suggest a strong synergistic activity of ST mixture against animal intestinal bacteria, The activity was considered to be comparable to those of other current antibiotics.

• 한국임상수의학회지
• /
• 제14권1호
• /
• pp.42-47
• /
• 1997

돼지의 호흡기감염은 양돈산업에서 과밀한 사육으로 인하여 발생되는 주요한 질환중의 하나이다. 호흡기감염을 일으키는 병원체들은 주로 기관분비물과 점액에 상주하는 것으로 알려져 있다. 그러므로 호흡기감염의 치료를 위하여 기관분비물내에 항균약제의 농도는 적절하게 유지되어져야 한다. sulfadiazine(SDZ)과 trimethoprim(TMP)의 혼합제제는 전세계적으로 돼지의 호흡기감염의 치료에 널리 사용되어지고 있으나, 기관분비물에서 약물의 동태학적 특성에 관한 연구는 거의 찾아 볼 수가 없었다. 본 연구는 돼지의 좌측전대정맥으로 SDZ/TMP혼합제제를 일회 투여한 후 형성되는 약물동태학적 매개변수를 알아보기 위하여 실시되었다. 혈장과 기관분비물에서 SDZ/TMP농도는 HPLC (High Performance Liquid Chromatography)로 측정하여 약물동태학적 매개변수를 산출하였다. 혈장에서 SDZ와 TMP의 약물동태학적 매개변수는 2-compartment 와 1-compartment모델에 적합하였다. 혈장내 TMP의 반감기(0.90$\pm $0.06시간)가 SDZ(7.25$\pm $1.09시간)에 비하여 매우 짧은 점이 주요한 차이점으로 볼 수 있었으며, 더욱이 투여후 8시간이후에서는 측정이 되지 않았다. 기관분비물에서 SDZ의 농도는 전체 실험기간(0.5∼32시간)동안 거의 일정하게 유지되었고, 16시간후에서는 혈장농도보다 높은 수준을 나타내었다. TMP농도는 투여후 2∼3시간에서 혈장농도보다 높게 유지되었다. 이러한 결과를 토대로 호흡기감염의 처치와 예방에 있어서 약물의 용량 및 투여빈도를 결정하는데 응용되어 질 수 있을 것이다.

• Journal of Microbiology and Biotechnology
• /
• 제12권1호
• /
• pp.106-113
• /
• 2002

One-hundred and twenty-four trimethoprim-resistant Shigella sonnei isolates extracted in Korea during the last two decades were investigated for their epidemiological relationship and mechanisms of resistance to trimethoprim. The S. sonnei isolates were distributed into two groups by three different epidemiological tools: biotyping, antibiogram, and pulsed-field gel electrophoresis. One group contained the isolates from the 1980s and the other group included the isolates from the 1990s. The geometric mean MICs of trimethoprim in S. sonnei isolates from the 1980s and 1990s were found to be $672.9{\mu}g/ml\;and\;>2,048{\mu}g/ml$, respectively. Trimethoprim resistance was associated with dfrA5, dfrA12, and dfrA13 genes in the isolates from the 1980s, dfrA1, dfrA5, and dfrA12 in the isolates from 1991, and dfrA1 and dfrA12 in the isolates from 1992 to 1999. The dfrA1 gene was located downstream of the intI2 gene in Tn7, which was located on chromosome. Some dfrA12 genes were found as gene cassettes in the class 1 integron. The dfrA5 and dfrA13 genes were located on conjugative plasmids. These results suggested that a clonal change occurred in S. sonnei isolates in Korea during the last two decades and that dfr genes located on different transposable genetic elements had gradually changed.

• 한국물환경학회지
• /
• 제34권2호
• /
• pp.149-156
• /
• 2018

Oxidation of Ciprofloxacin, Trimethoprim, and Enrofloxacin by ozone was experimentally investigated to observe the effects of background water quality (such as ultrapure water, humic acid, and biologically treated wastewater) and water temperature on the removal rate of these antibiotics, and, thereby, to be able to provide design information when the ozone treatment process is adopted. Initial concentrations of the antibiotics spiked to $10{\mu}g/L$, and the ozone dose was 1, 2, 3, 5, and 8 mg/L. While the removal rate of Ciprofloxacin under ultrapure water background by ozone oxidation was over 99%, the removal rate under humic acid and biologically treated wastewater background was markedly lower, in the range of 49.3% ~ 99% and 19.8 % ~ 99 %, respectively. When water temperature is decreased from $20^{\circ}C$ to $4^{\circ}C$, the removal rate is reduced from the range of 19.8% ~ 99 % to the range of 7.5 % ~ 99 % under a biologically treated wastewater background. The effects of background and temperature on the removal rate of Trimethoprim and Enrofloxacin were similar to that of Ciprofloxacin, but the degree was different. Therefore, it is concluded that the background of water to be treated, as well as water temperature, should be taken into consideration when the design factor, such as ozone dose, is determined, so that the treatment objective of the ozone treatment process can be most effectively met.

• 현장농수산연구지
• /
• 제22권2호
• /
• pp.51-58
• /
• 2020

2019년 11월, 국내의 뱀장어 양식장에서 양식 중이던 뱀장어가 피부궤양 및 피부의 점상출혈 소견을 보이며 지속적으로 폐사하였다. 일련의 폐사는 2019년 11월부터 12월까지 지속되었다. Vibrio 속 균주 1E1-2는 첫 번째 폐사 사례에서 폐사한 뱀장어의 출혈성 복수에서 분리되었고 균주 2A3-1는 두 번째 폐사 사례에서 폐사한 뱀장어의 복수에서 분리되었으며 균주 3K1-2는 세 번째 폐사 사례에서 폐사한 뱀장어의 신장에서 분리되었다. 16S rRNA gene 시퀀스 분석으로, 분리균주 1E1-2와 3K1-2가 V. fluvialis NBRC 103150T와 가장 높은 유사도를 나타내며 V. fluvialis로 동정되었으며, 균주 2A3-1는 V. plantisponsor NBRC103148T와 가장 높은 유사도를 나타내며 V. plantisponsor로 확인되었다. 항생제 감수성 실험 결과로, V. fluvialis 1E1-2는 tetracycline과 chloramphenicol에 대하여 중등도의 감수성을 보였고 trimethoprim-sulfamethoxazole에 대하여 내성을 나타내었다. V. plantisponsor 2A3-1는 ciprofloxacin과 levofloxacin에 대하여 중등도의 감수성을 나타내었고 trimethoprim-sulfamethoxazole에 대하여 내성을 나타내었다. V. fluvialis 3K1-2는 tetracycline에 대하여 중등도의 감수성을 나타내었고 ampicillin과 trimethoprim-sulfamethoxazole에 대하여 내성을 나타내었다. 이는 국내 양식 뱀장어에서의 항생제 내성 비브리오 감염증 사례로, 의미 깊은 보고라고 할 수 있다.