• Korean Journal of Pharmacognosy
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• v.37 no.4 s.147
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• pp.266-271
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• 2006

Sophorae Radix, the dried roots of Sophora flavescens Aiton (Leguminosae), has been used in oriental traditional medicine for treatment of skin and mucosal ulcers, sores, gastrointestinal hemorrhage, diarrhea, inflammation and arrhythmia. Present study was carried out for the gastroprotective effect of trifolirhizin from Sophora flavescens. This reports were evaluated antibacterial activity against Helicobacter pylori and HCl ethanol-induced gastric lesion in rats and showed the significant effectiveness. In pylorus ligated rats, the treatments of trifolirhizin showed decrease in the volume of gastric secretion and acid output. Also we evaluated the antibacterial activity against H. pylori treated with methanol extract and trifolirhizin from Sophora flavescens had a equivalent antibacterial activity with ampicillin against H. pylori at the dose of $100{\mu}g/mL$. It may be regarded that the antigastritic effects and antibacterial activity of trifolirhizin from Sophora flavescens are originated from reduction of total acid output identified by gastric secretion reduce, free radical scavenging effects and the antibacterial activity against H. pylori.

• Natural Product Sciences
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• v.13 no.3
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• pp.255-257
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• 2007

A simple reversed phase HPLC method was developed for extracting pharmacologically active compounds kurarinone and trifolirhizin from the roots of Sophora flavescens using a binary gradient of acetonitrile : $H_{2}O$ with UV detection at 254 nm. The kurarinone and trifolirhizin contents of the roots of S. flavescens collected from ten district markets in Korea and China were $77.24{\sim}686.89$ ${\mu}g/g$ and $46.89{\sim}288.58$ ${\mu}/g$, respectively.

• Journal of the Korean Wood Science and Technology
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• v.31 no.3
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• pp.70-76
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• 2003

Five isoflavonoids, biochanin A-7-O-β-D-xylopyranosyl-(1⟶6)-β-D-gluco- pyranoside (1), (-)-maackiain (2), calycosin (3), trifolirhizin (4) and genistein (5), were tested for antifungal activity against nine fungi. These compounds were isolated from the wood (compound 1 and 2) and from the bark (compound 3, 4 and 5) of S. japonica. According to the results of antifungal activity test, (-)-maackiain was evaluated as the best antifungal compound among the isolated compounds. In this regard, it could be mentioned that high antifungal activity of S. japonica wood extracts was originated from (-)-maackiain.

• Journal of the Korean Wood Science and Technology
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• v.30 no.3
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• pp.42-47
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• 2002

This study was carried out to investigate the constituents of Sophora japonica(Leguminosae) bark. To isolate compounds, bark was extracted with ethanol and then partitioned with hexane, dichloromethane, ethylacetate and butanol successively. After partitioned, DCM fraction was subjected to column chromatography with various solvent system in silica gel and/or Sephadex LH-20. Structures were elucidated by spectroscopic methods including MS, ¹H, ¹³C and 2D-NMR experiments. Four compounds were isolated from the bark and identified as 3',7-dihydroxy-4'-methoxyisoflavone (cyclosin), puerol A, maackiain-3-O-𝛽-D-glucopyranoside (trifolirhizin), and 4', 5, 7-trihydroxyisoflavone (genistein). Among these compounds, cyclosin and trifolirhzin were first isolated from S. japonica.

• Archives of Pharmacal Research
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• v.26 no.12
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• pp.1009-1013
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• 2003

A new pterocarpan, (-)-maackiain 3-sulfate (1) was isolated from the methanol extract of roots of Sophora subprostarata together with (-)-maackiain(2), trifolirhizin (3), lupeol (4), ononin (5),7,4'-dihydroxyflavone (5), and (+)-syringaresinol (7). The structure of 1 was determined by analyses of 2D NMR and HRFABMS. Compounds 5-7 were isolated from this plant for the first time.

• Archives of Pharmacal Research
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• v.28 no.2
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• pp.190-194
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• 2005

In our search for monoamine oxidase (MAO) inhibitors from natural resources, we found that the methanol extract of the roots of Sophora flavescens showed an inhibitory effect on mouse brain monoamine oxidase (MAO). Bioactivity-guided isolation of the extract yielded two known flavonoids, formononetin (1) and kushenol F (2), as active compounds along with three inactive compounds, oxymatrine (3), trifolirhizin (4), and ${\beta}$-sitosterol (5). Formononetin (1) and kushenol F (2) showed significant inhibitory effects on MAO in a dose-dependent manner with $IC_{50}$ values of 13.2 and $69.9\;{\mu}M$, respectively. Formononetin (1) showed a slightly more potent inhibitory effect against MAO-B ($IC_{50}:\;11.0\;{\mu}M$) than MAO-A ($IC_{50}:\;21.2\;{\mu}M$). Kushenol F (2) also preferentially inhibited the MAO-B activity than MAO-A activity with the $IC_{50}$ values of 63.1 and $103.7\;{\mu}M$, respectively.

• Archives of Pharmacal Research
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• v.28 no.5
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• pp.534-540
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• 2005

Activity-guided fractionation of the CH$_2Cl_2$-soluble fraction of the roots of Sophora flavescens furnished five 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scaveng ers: trans-hexadecyl ferulic acid (1) cis-octadecyl ferulic acid (2), trans-hexadecyl sinapic acid (3), (-)-4-hydroxy-3-methoxy-(6aR,11aR)-8, 9-methylenedioxypterocarpan (4) and desmethylanhydroicaritin (8), along with nine known inactive compounds: (-)-maackiain (5), xanthohumol (6), formononetin (7), (2S)-2'-methoxykurarinone (9), (2S)-3${\beta}$,7,4'-trihydroxy-5-methoxy-8-(${\gamma},{\gamma}$- imethylallyl )-flavanone (10), (2S)-7,4'-dihydroxy-5-methoxy-8- (${\gamma},{\gamma}$-dimethylallyl ) -flavanone (11), umbelliferone (12), kuraridin (13), and trifolirhizin (14). Compounds 1-4 and 8 exhibited DPPH free radical scavenging effects at IC$_{50}$ values of 33.01 ${\pm}$ 0.20, 57.06 ${\pm}$ 0.16, 39.84 ${\pm}$ 0.36, 35.83 ${\pm}$ 0.47, and 18.11 ${\pm}$ 0.04${\mu}$M, respectively. L-Ascorbic acid, when used as a positive control, exhibited an IC$_{50}$ value of 7.39 ${\pm}$ 0.01 ${\mu}$M. Compounds 1-4 and 8 also appeared to exert significant scavenging effects on authentic ONOO-, with IC$_{50}$ values of 5.76 ${\pm}$ 1.19, 15.06 ${\pm}$ 1.64, 8.17 ${\pm}$ 4.97, 1.95 ${\pm}$ 0.29 and 4.06 ${\pm}$ 2.41 ${\mu}$M, respectively. Penicillamine (IC$_{50}$= 2.36 ${\pm}$ 0.79${\mu}$M) was used as a positive control. In addition, compounds 2,4,6,8, and 10 were isolated from this plant for the first time.