• 한국균학회지
• /
• 제49권4호
• /
• pp.469-476
• /
• 2021

송이는 상업적으로 중요한 야생 식용 버섯이다. 현재까지 송이 재배는 송이감염묘를 이용한 것이 유일한 방법이다. 2020년 송이감염묘로부터 21개의 송이가 홍천 송이시험지에서 발생하였다. 송이감염묘 생산지역의 송이와 이식한 송이감염묘로부터 발생한 송이는 모니터링을 통해 확인하였고, 13개의 microsatellite 마커를 이용하여 분석한 결과 동일한 것이 확인되었다. 송이감염묘는 송이균환 형성과 송이 발생에 성공하였고, 송이발생은 2010년과 2017년부터 2020년까지 발생하였다. 결론적으로 송이 버섯발생은 송이감염묘의 송이균에서 기원한 것이다.

• Mycobiology
• /
• 제35권2호
• /
• pp.54-61
• /
• 2007

Two isolates of Tricholoma matsutake T-008 and T-034, preserved in Entomopathogenic Fungal Culture Collection (EFCC) of Korea, were used in the present study. The isolates had 100% Bootstrap homology with Tricholoma matsutake U62964 and T. matsutake AB188557 and AF309538 preserved in Gene Bank of NCBI. Mycelial growth of T. matsutake was highest in TMM and MYA at $25^{\circ}C$. The highest dry wt. of mycelium was obtained after 65 days of culture, when 6 mycelial discs were inoculated in 100 ml of broth in 250 ml shaking flask. Mycelial mats were observed in clumped condition at the inoculation sites of pine forest after two weeks of inoculation. After 5 months of inoculation, mycelia mats were observed growing inside soil and walls of a few inoculation sites, while mycelial mats growth up to $5{\sim}8$ cm were observed in the roots of pine tree after 6 months. The survival rate of the inoculum was about 40% of the total inoculation sites. The survival rate was found below 20% when the mycelium was inoculated in the summer. The reasons for low survival rates of the mycelium were mainly due to dry season and the soil-borne small animals such as earthworm and mole. After one year of inoculation, no external difference was observed between the artificially inoculated mycelia and the naturally existing mycelia of T. matsutake. The present study showed that fruiting bodies of T. matsutake could be produced by artificial inoculation under the appropriate environmental conditions.

• Mycobiology
• /
• 제29권4호
• /
• pp.183-189
• /
• 2001

Extracellular amylase properties were examined with the mycelium of Tricholoma matsutake isolated from ectomycorrhizal roots of Pinus densiflora. The molecular weights of $\alpha$-amylase and glucoamylase were estimated as 34.2 kD and 11.5 kD, respectively, after eluted through Superdex 75 column. The optimum pH of the purified enzyme was found in a range of pH $5.0{\sim}6.0$, with a peak at pH 5.0. The activities of these enzymes were stable from $4^{\circ}C\;to\;30^{\circ}C$. The $\alpha$-amylase of T. matsutake readily hydrolyzed soluble starch and amylose-B, while it weakly hydrolyzed glycogen, dextrin, amylose and amylose-A. The main products of hydrolysis were confirmed to be glucose, maltose and maltotriose on the basis of the similarities in the thin layer chromatographic mobility.

• 한국균학회지
• /
• 제20권2호
• /
• pp.144-148
• /
• 1992

야생(野生) 버섯 중(中) 송이의 대표적 발생지(發生地)인 전북(全北) 남원(南原) 지역(地域)에서 식생(植生) 및 주요(主要) 야생(野生)버섯 분포(分布)를 조사(調査)한 결과(結果)는 다음과 같다. 송이 발생지(發生地)에서 식생(植生)의 출현종수(出現種數)는 $11{\sim}29$종(種)이었으며 교목층(喬木層)(Tl, T2)에서는 소나무(Pinus densiflora S. et. Z.), 관목층(灌木層)(S)에서는 진달래(Rhododendron mucronulatm TURCZ), 졸참나무(Quercus serrata THUNB.), 초목층(草木層)(H)에서는 산거울(Carex humilis LEYss.)이 우고(優古)하고 있었다. 송이 발생지(發生地)의 주요(主要) 야생(野生) 버섯 분포(分布)는 그물버섯과(科)(Boletaceae)에 속하는 평원비단그물버섯(Suilus Placidus Bon. Sing.)과 싸리버섯과(科)(Ramariaceae)에 속하는 싸리버섯(Ramaria botrytis Fr. Ricken) 등(等)이 주종(主從)을 이루었다.

• Journal of Pharmaceutical Investigation
• /
• 제19권3호
• /
• pp.137-143
• /
• 1989

This study was attempted to investigate the effect of Tricholoma matsutake on experimentally induced hyperlipemia in rats. The levels of cholesterol, triglyceride and phospholipid were measured. Diagnostic attention has been paid to the cholesterol concentration associated with high density lipoprotein (HDL), which appears to be inversely related to the incidence of coronary artery disease. For these reasons, determination of HDL-cholesterol has significant meaning. Total cholesterol was determined by Abell-Kindall method, and to measure HDL-cholesterol, serum low density lipoproteins (LDL) were first selectively precipitated by HDL-precipitating reagent. The results obtained were as follows : 1) The level of total cholesterol in serum of hyperlipemic rats was decreased by oral administration of Tricholoma matsutake extract. 2) The level of HDL-cholesterol was increased by the mushroom extract. 3) The level of triglyceride was significantly decreased by the mushroom extract. 4) The level of phospholipid was slightly decreased by the mushroom extract.

• 동의생리병리학회지
• /
• 제22권3호
• /
• pp.672-677
• /
• 2008

In order to explore the anti-oxidization effect of oriental medicine and cereal medium(OCM) where Tricholoma matsutake mycelia were cultured, measurement of hot water extract and UMPM(extraction method using ultra sonic waves, micro waves, micro bubble) extract, the total polyphenol content of crude polysaccharide from each extract, SOD-like activity, electron donating ability(EDA), xanthine oxidase inhibitory activity, and tyrosinase inhibitory activity was conducted. The total polyphenol content of each extract was found to be 16.36% for hot water extract(HE) group and 15.73% for UMPM extract(UE) group and the amount of crude polysaccharide precipitated into ethanol of extracts was found to be 8.79% for UMPM ethanol extract(UEE) group and 6.48% for hot water ethanol extract(HEE) group. As a result of measurement of SOD-like activity by concentration of each extract, it was found to be 96.17% for UE group, 91.23% for HE group, 91.33% for UEE group, and 87.11% for HEE group at 20 mg/mL. In the case of EDA, it was found to be 47.55% for UE group, 44.93% for HE group, 25.38% for UEE group, and 18.36% for HEE group. And in the cases of the rates of xanthine oxidase inhibitory activity and tyrosinase inhibitory activity, as the concentration of each extract increased, the inhibition rate increased accordingly. As a result of comparison between hot water extract method and UMPM extract method using extracts obtained from oriental medicine compound medium where Tricholoma matsutake mycelia were cultured, all of the extracts were judged to have a high anti-oxidization effect. In particular, UMPM extracts were found to have higher polyphenol content, SOD-like activity, EDA, xanthine oxidase inhibitory activity and tyrosinase inhibitory activity compared to hot water extract method. In this regard, extracts obtained from OCM where Tricholoma matsutake mycelia were cultured are considered to have high availability as functional material when and if they are prepared using UMPM extract method.

• 농업과학연구
• /
• 제40권1호
• /
• pp.13-18
• /
• 2013

Effect of electric pulse stimulation was tested on the fruit body formation of valuable mushroom Tricholoma matsutake in the field of natural habitat of this mushroom. After applying the electric stimulator to the specific area of pine forest we found that the treatment especially stimulated the fructification of T. matsutake. And the most valuable findings of our study was that only our treatment plots showed fruit body formation whereas the control plots and the whole natural habitats of our study area showed zero production of this mushroom during the same time. From the point of view of mushroom production, our experiment shows that fruit body production can be upgraded by using pulsed power as an electrical stimulation in the field of the natural habitat of this mushroom. These findings from our experiment confirm the effectiveness of the significance of pulsed power technology for the improvement of T. matsutake fruit body production in the natural habit of this mushroom.

• 한국산림과학회지
• /
• 제89권2호
• /
• pp.232-240
• /
• 2000

송이(Tricholoma matsutake, Songyi, matsutake) 생산과 소나무 생장과의 관계를 알기 위하여, 경북 상주지역의 18년(1978-1995)간의 송이 생산량과 인접 속리산의 소나무 연륜생장과 상관관계를 분석하였다. 두 변수간의 상관관계는 r=0.408로써 통계적으로 유의하지 않았다. 상관관계가 낮은 이유로는 송이생산 변동은 버섯발생 및 생장시기인 9월과 10월의 기상에 영향을 받는 데 비하여 소나무 연륜생장 변동은 5월과 6월의 기상에 주로 영향을 받기 때문으로 생각되었다. 1999년의 경우 충북 월악산 국립공원내 송이생산이 절정을 이룬 시기는 10월 초 1주일간으로 이때 일최저기온은 약 $7^{\circ}C-13^{\circ}C$이었다. 이러한 것은 송이생산의 풍-흉년이 단순한 해거리 현상이 아님을 의미한다. 송이증산을 위해서는 자실체 발생기간 중에 발생지의 토양수분과 온도조절이 중요하다고 생각한다.

• Journal of Microbiology and Biotechnology
• /
• 제30권2호
• /
• pp.296-305
• /
• 2020

Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)-1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)-1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.

• Current Research on Agriculture and Life Sciences
• /
• 제20권
• /
• pp.77-82
• /
• 2002

This study was carried out in order to produce useful material for the forest multiple use and forest protection by physico-chemical soil analysis of studied area in Sokwang-ri Forest Genetic Resource Protection Forest which was divided into in standard plots include Tricholoma matsutake and Sarcodon aspratus production forest. The result of physico-chemical soil analysis represented as following. The soil type of T. matsutake production forest was Dry brown forest soil(B1), while on the other hand the soil type of S. aspratus production forest was Moderately moist brown forest soil(B3). Between T. matsutake and S. aspratus production forest did not result in significant changes in soil pH(5.22-5.60) and soil depth(47cm), but available phosphorus, carbon, and nitrogen contents were different results. CN ratio of the fairy ring of T. matsutake was quite lower than that in S. aspratus production forests, which indicated that T. matsutake production forest was built up in the relatively immature soils which contain little organic matter. Generally, it was predicted that Pinus densiflora for. erecta forest succeeded to deciduous tree forest in stable soil environments. To conserve these T. matsutake and S. aspratus production forest, the contents of available phosphorous and exchangeable cation should be increased by continuous soil environment management and it should be established the secondary growth forests of old aged Pinus densiflora for. erecta trees as soon as possible.