• Title/Summary/Keyword: tributylin

Search Result 3, Processing Time 0.24 seconds

Tributyltin chloride(TBTC)와 Triphenyltin chloride(TPTC)에 노출된 넙치(Paralichthys olivaceus) 치어에 있어서의 RT-PCR에 의한 유전자 증폭반응

  • 최윤실;함승협;진덕희;전중균
    • Proceedings of the Korean Society of Fisheries Technology Conference
    • /
    • 2000.05a
    • /
    • pp.373-374
    • /
    • 2000
  • Tributylin(TBT)과 Triphenyltin(TPT) 같은 유기주석화합물은 선박, 어망, 어구등 방오도료의 소재로 사용되어 왔으나 최근 해양생물에게 미치는 독성 때문에 세계 각 국에서도 그 사용을 금지하고 있다. 우리 나라의 경우, 한국 해양연구소가 내분비 교란물질(환경호르몬)의 농도를 조사한 결과 다른 지역에 비해 내만 지역에서의 오염도가 다소 높다고 보고되어 있다. 내만지역의 경우는 파도가 약하여 양식장이 많으므로 양식어류가 TBT와 같은 유기주석 화합물에 노출될 가능성은 매우 높다. (중략)

  • PDF

Purification and Biochemical Analysis of Rice Bran Lipase Enzyme

  • Kim, Young Hee
    • Journal of Plant Biotechnology
    • /
    • v.6 no.1
    • /
    • pp.63-67
    • /
    • 2004
  • A simple procedure for the extraction of the lipolytic enzyme from rice bran has been developed. High activity of lipolytic enzyme was obtained by first defatting the rice bran to remove lipid components with various extraction conditions. Then, after rove cycles of aqueous extraction, rice bran lipolytic enzyme was purified using micro- and ultrafiltration apparatus. Lipolytic enzyme activity was estimated by its hydrolytic action of tributyrin. The result indicated that the standard activity curve of butyric acid showed that the potential rice bran enzyme is a hydrolytic lipase enzyme. In addition, it showed higher lipolytic activity and specific enzyme activity with further purification by micro- and ultrafiltration. The size of rice bran lipase enzyme was identified through 15 % SDS-PAGE. The molecular weight of the rice bran lipase enzyme was 41 kDa.

Temporal and Spatial Distribution of Butyltin Compounds in Marine Sediments from Ulsan Bay, Korea (울산만 퇴적물 내 유기주석화합물의 시공간적 분포 특성)

  • Baek, Seung-Hong;Choi, Mminkyu;Lee, In-Seok;Lee, Jae-Hwan;Hwang, Dong-Woon;Kim, Sook-Yang
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
    • /
    • v.19 no.1
    • /
    • pp.1-7
    • /
    • 2014
  • Butyltin compounds (BTs) were determined in surface sediment samples from Ulsan Bay in 2009 and 2011, to assess the contamination status and the effectiveness of tributylin (TBT) restriction of Korea. Dibutyltin and TBT concentrations in sediments in 2011 ranged from 19.5 to 980 and 14.2 to 1204 ng Sn $g^{-1}$ dry weight, respectively. The highest TBT concentration was found in a big shipyard complex and the next highest was found in harbor, suggesting that harbor and shipyard activities are a major contamination source of TBT in Ulsan Bay. Concentrations of BTs in Ulsan Bay sediments were significantly lower in 2009 and 2011 than that in 2003 survey previously reported, probably as a consequence of the legislative action in Korea. However, TBT concentrations in sediments still exceeded ecotoxicological values. Therefore, it will be important to monitor and reduce TBT contamination in sediments until the levels fall well below ecotoxicological levels.