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Improved Antibacterial Effect of Blending Essential Oils (블렌딩 에센션오일의 항균효과 증진)

  • Kwon, Pil Seung;Kim, Dae-Jung;Park, Ho
    • Korean Journal of Clinical Laboratory Science
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    • v.49 no.3
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    • pp.256-262
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    • 2017
  • Essential oil from herb is known to exert pharmacological effects on the human body. In this study we investigated the antibacterial activity of 4 essential oils (teetree, rosemary, melisa, and lavender), as well as the blended mixture oil of teetree, rosemary, and melisa (TRM) on three bacteria, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Antibacterial analysis was performed using the standard disk diffusion method, and minimum inhibition concentration was determined by the broth microdilution method with different concentrations of essential oils (0.5, 1, 2 and 3 mg/mL). After incubation at $37^{\circ}C$ for 24 h, the antibacterial activity was assessed by measuring the zone of growth inhibition surrounding the disks. Herb oil with the inhibition zones showed varied values ranging from6 to 25 mm. However, the components of herb oil of TRM are as highly active as the teetree oil against pathogens, generating large inhibition zones for both gram negative and positive bacteria (13~22 mm and 8 mm inhibition zones). In the analysis for MIC, TRM showed growth-inhibitory effects at 0.0625% for S. aureus and E. coli, and 1.25% for P. aeruginosa. This result demonstrated that the anti-microbial activity of TRM was greater than a single herb oil, including oxacillin, rosemary, and teetrea. As a single herb oil, both rosemary and teetrea also had an anti-microbial effect by itself, and we can expect that the blended oil mixture may exert a synergistic effect against multidrug resistant bacteria, suggesting its future application in natural preservative agents for health food and cosmetics.

A Development of Stem Analysis Program and its Comparison with other Method for Increment Calculation (수간석해(樹幹析解) 전산(電算)프로그램 개발(開發) 및 생장량(生長量) 계산방법(計算方法)의 비교(比較)에 관(關)한 연구(硏究))

  • Byun, Woo Hyuk;Lee, Woo Kyun;Yun, Kwang Bae
    • Journal of Korean Society of Forest Science
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    • v.79 no.1
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    • pp.1-15
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    • 1990
  • In this study the stem analysis program, which can be operated with personal computer was developed to reduce time and cost of calculation, and to increase accuracy of analysis. The stem analysis method used in this program was compared with other methods. The results obtained were as follows : The value, 1/100mm measured from the latest annual ring measurement machine (Jahrringme${\beta}$geraete Johan Type II) was automatically inputed to the computer and saved into given file name. Turbo Pascal program was written to do this. The measured data was analyzed by stem analysis calculation program written by Fortran-77. Volume and height increments were approximated by spline function, and diameter of the stem disk was calculated by quadratic mean method. The increment values calculated by the programs were printed annually and in every five-year. Stem analysis diagram and several increment graphs were also easily printed. The result compared between those analysis methods showed that quadratic mean could reduce the error caused from eccentric pith. When the stem taper curve method, approximated by spline function, was used in the calculation of tree height and volume, increments would be more exactly calculated.

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Development of Stem Analysis Program(Stemwin1.0) for Windows (Windows용 수간석해(樹幹析解) 프로그램(Stemwin1.0)의 개발(開發))

  • Lee, Joon-Hak;Lee, Woo-Kyun;Seo, Jeong-Ho
    • Journal of Korean Society of Forest Science
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    • v.90 no.3
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    • pp.331-337
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    • 2001
  • This study was performed to develope stem analysis program(Stemwin1.0) which can be used in PC with MS-Windows operating system. Stemwin1.0 uses width of annual tree ring measured with 1/100mm unit, and calculate increments of several growth factors such as DBH, height and volume with various methods. Mean DBH can be calculated by arithmetic and quadratic mean methods. Height can be estimated by parallel line, line extending and height curve methods. Volume can be estimated by Huber, Smalian, and Spline functions. Not only Total growth, Mean Annual Increment(MAI) and Current Annual Increment(CAI) of growth factors, but also merchantable volume and height, form factor, growth rate, and merchantable volume rate are automatically calculated. Stemwin1.0 can also output accurate stem taper curve with various scale, and prepare stem taper data(diameter at different disk heights) for statistical analysis for deriving stem taper model. Stemwin1.0 can export output data and graph to Excel for more compatible use of it.

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Optimization of Post-Processing for Subsequence Matching in Time-Series Databases (시계열 데이터베이스에서 서브시퀀스 매칭을 위한 후처리 과정의 최적화)

  • Kim, Sang-Uk
    • The KIPS Transactions:PartD
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    • v.9D no.4
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    • pp.555-560
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    • 2002
  • Subsequence matching, which consists of index searching and post-processing steps, is an operation that finds those subsequences whose changing patterns are similar to that of a given query sequence from a time-series database. This paper discusses optimization of post-processing for subsequence matching. The common problem occurred in post-processing of previous methods is to compare the candidate subsequence with the query sequence for discarding false alarms whenever each candidate subsequence appears during index searching. This makes a sequence containing candidate subsequences to be accessed multiple times from disk, and also have a candidate subsequence to be compared with the query sequence multiple times. These redundancies cause the performance of subsequence matching to degrade seriously. In this paper, we propose a new optimal method for resolving the problem. The proposed method stores ail the candidate subsequences returned by index searching into a binary search tree, and performs post-processing in a batch fashion after finishing the index searching. By this method, we are able to completely eliminate the redundancies mentioned above. For verifying the performance improvement effect of the proposed method, we perform extensive experiments using a real-life stock data set. The results reveal that the proposed method achieves 55 times to 156 times speedup over the previous methods.

Changes in Dormant Phase and Bud Development of 'Fuji' Apple Trees in the Chungju Area of Korea (충주지역에서 '후지' 사과나무의 휴면단계 변화 및 눈 발달)

  • Lee, ByulHaNa;Park, YoSup;Park, Hee-Seung
    • Horticultural Science & Technology
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    • v.33 no.4
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    • pp.501-510
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    • 2015
  • In this study, we investigated the onset and release of endo-dormancy under natural conditions by observing bud break characteristics in 'Fuji' apple trees using water cuttings. Through examinations of bud break rate and days to bud break, we found that the endo-dormancy of 'Fuji' apple tree continues for 70 d from 165 to 255 d after full bloom (DAFB), from late October to early January of the following year. In addition, within 20 d of first bud break, based on a final bud break rate of 60% or more, we able to identify the timing of the changeover from para-dormancy to endo-dormancy, and endo-dormancy to eco-dormancy. Analysis of the chilling requirement during the endo-dormancy period revealed that chilling accumulation up to 255 DAFB to release endo-dormancy amounted to 666 and 517 h based on the CH and Utah models, respectively. Observation of internal changes in the bud during endo-dormancy showed that flower bud differentiation begins from mid-July, and t ime of inflorescence o f the disk f lower is a vailable to f ind. The f lower buds subsequently developed slowly but steadily during endo-dormancy and in the following year in February, the developmental stage of each organ had progressed. Moreover, the flower buds of 'Fuji' apples were mostly healthy during the dormancy period, but some exhibited necrosis of flower primordium, due partial cell damage from the formation of ice crystals rather than a direct effect of the low temperature. Flower buds were formed in both the axillary buds of bourse shoots and terminal buds of spurs, but lower bud differentiation was observed for the terminal buds of spurs at rate of about 65% of total buds, which was directly related to the bud size and shoot diameter.