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Genetic Variation of Parental Inbred Lines for Korean Waxy Corn Hybrid Varieties revealed by SSR markers (우리나라 찰옥수수 품종들의 교배친 자식계통들에 대한 유전적 변이성)

  • Park, Jun-Sung;Sa, Kyu-Jin;Park, Ki Jin;Jang, Jin-Sun;Lee, Ju Kyong
    • Korean Journal of Breeding Science
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    • v.41 no.2
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    • pp.106-114
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    • 2009
  • In maize, knowledge of genetic diversity and genetic relationships among elite inbred lines is an significant impact on the selection of parental lines for hybrid varieties. Genetic diversity and genetic relationships among 11 parental inbred lines of Korean waxy and normal corn varieties were analyzed using 50 SSR markers distributed over the whole genome. A total of 171 allele bands were detected with an average of 3.4 alleles per locus. Number of allele bands per locus ranged from two to six and gene diversity varied from 0.165 to 0.900 with an average of 0.596 depending on the SSR loci. The cluster tree recognized three major groups with 61.6% genetic similarity. Group I includes 7 inbred lines (KL103, HW1, HW4, HW6, HW7, HW8, HW9), with similarity coefficients of between 0.616 and 0.730. Group II includes 2 inbred lines (HF1, HF2), with similarity coefficients of 0.959. Group III includes 2 inbred lines (HW3, HW5), with similarity coefficients of 0.713. The present study indicates that the SSR markers chosen for this analysis are effective for the assessment of genetic diversity and genetic relationships among 11 parental inbred lines.

Analysis of Genetic Diversity and Population Structure for Core Set of Waxy and Normal Maize Inbred Lines using SSR Markers (SSR 분자마커를 이용한 찰옥수수 및 종실용 옥수수 자식계통들의 핵심집단에 대한 유전적 다양성 및 집단구조 분석)

  • Sa, Kyu Jin;Kim, Jin-Ah;Park, Ki Jin;Park, Jong Yeol;Goh, Byeong Dae;Lee, Ju Kyong
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.430-441
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    • 2011
  • Maize is divided into two types based on the starch composition of the endosperm in the seed, normal maize(or non-waxy maize) and waxy maize. In this study, genetic diversity and population structure were investigated among 80 waxy maize and normal inbred lines(40 waxy maize inbred lines and 40 normal maize inbred lines) using 50 SSR markers. A total of 242 alleles were identified at all the loci with an average of 4.84 and a range between 2 and 9 alleles per locus. The gene diversity values varied from 0.420 to 0.854 with an average of 0.654. The PIC values varied from 0.332 to 0.838 with an average of 0.602. To evaluate the population structure, STRUCTURE 2.2 program was employed to confirm genetic structure. The 80 waxy and normal maize inbred lines were separated with based on the membership probability threshold 0.8, and divided into groups I, II and admixed group. The 13 waxy maize inbred lines were assigned to group I. The 45 maize inbred lines including 7 waxy maize inbred lines and 38 normal maize inbred lines were assigned to group II. The 22 maize inbred lines with 20 waxy maize inbred lines and 2 normal maize inbred lines were contained in the admixed group. The cluster tree generated using the described SSR markers recognized three major groups at 31.7% genetic similarity. Group I included 40 waxy maize inbred lines and 11 normal maize inbred lines, and Group II included 27 normal maize inbred lines. Group III consist of only 2 normal maize inbred lines. The present study has demonstrated the utility of SSR analysis for the study of genetic diversity and the population structure among waxy and normal maize inbred lines. The information obtained from the present studies would be very useful for designing efficient maize breeding programs in Maize Experiment Station, Kangwon Agricultural Research and Extension Services.

Studies on the Construction Method of Chwibyeong and Investigating Original Form of the Chwibyeong at the Juhapru in the Changdeok Palace (취병(翠屛)의 조성방법과 창덕궁 주합루(宙合樓) 취병의 원형규명)

  • Jung, Woo-Jin;Sim, Woo-Kyung
    • Korean Journal of Heritage: History & Science
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    • v.47 no.2
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    • pp.86-113
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    • 2014
  • This study has researched the characteristics and elements of Chwibyeong (翠屛), a sort of trellis in the Joseon Dynasty through the old documents, and the original form of Chwibyeong at Juhapru (宙合樓) in Changdeuk Palace. The results were as follow. First, as the result of literatures analysis for Imwon-gyeongje-ji (林園經濟志) and Jeungbo-sallim-gyeongje (增補山林經濟), the plant screen was classified as kinds of support[frame] material, plants and methods of planting. It was found that the supports of Chwibyeong were made of bamboo or the material such as the Jinjangmok (眞長木: a stick of oak) and Giryu (杞柳: Salix purpurea var. japonica). The evergreen coniferous trees including Pinus densiflora, Taxus cuspidata and Thuja orientalis were mainly used for the plant material of Chwibyeong. The general planting method of Chwibyeong was to plant on the ground, but sometimes the container planting was also found on the artificial ground. Second, the term of 'Chwibyeong' in the literatures was used in only the screen made by evergreen trees, and the superordinate category term of it was indicated by 'byeong (屛)'. Therefore Chwibyeong was a compound word formed from 'chwi (翠)' which means the characteristics of evergreen and 'byeong' as tree screen which the support was made by bamboo. And Chwibyeong had semantic context which was combined with the literary symbolization to describe a landscape of green peak and Taoist ideology be inherent from 'twelve peaks of Musan[巫山十二峰]' in Sichuan sheng (四川省). Thirdly, the photograph of Chwibyeong at Juhapru taken by the 1880s, showed that Chwibyeong was made with coniferous trees and was almost 2 meters high. The Chwibyeong at Juhapru was removed during the Japanese colonial era, but a few yew trees(Taxus cuspidata) used for Chwibyeong are still remaining. And some Juniperus chinensis which the composition time is unclear, were cultivated while hung loose its branchs at the sides of Eosumun (魚水門). This Junipers were presumed to be planted by Japanese after Japanese annexation of Korea(1910), and it was judged that both of the roofs of Eosumun's side gates might have been transformed into Japanese style at the same time. Lastly, Chwibyeong at Juhapru was restored in 2008 but it was restored in wrong way from original form without precise research. Especially Chwibyeong was restored with Sasa boreralis which is damaged by frost, so it requires exertion that should revive the originals to plant original material as much as possible. And it needs the development of fabrication technique for Chwibyeong and the application to current landscape architecture.

Estimation of the Three-dimensional Vegetation Landscape of the Donhwamun Gate Area in Changdeokgung Palace through the Rubber Sheeting Transformation of (<동궐도(東闕圖)>의 러버쉬팅변환을 통한 창덕궁 돈화문 지역의 입체적 식생 경관 추정)

  • Lee, Jae-Yong
    • Korean Journal of Heritage: History & Science
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    • v.51 no.2
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    • pp.138-153
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    • 2018
  • The purpose of this study was to analyze , which was made in the late Joseon Dynasty to specify the vegetation landscape of the Donhwamun Gate area in Changdeokgung Palace. The study results can be summarized as below. First, based on "Jieziyuan Huazhuan(芥子園畵傳)", the introductory book of tree expression delivered from China in the 17th century, allowed the classification criteria of the trees described in the picture to be established and helped identify their types. As a result of the classification, there were 10 species and 50 trees in the Donhwamun Gate area of . Second, it was possible to measure the real size of the trees described in the picture through the elevated drawing scale of . The height of the trees ranged from a minimum of 4.37 m to a maximum of 22.37 m. According to the measurement results, compared to the old trees currently living in Changdeokgung Palace, the trees described in the picture were found to be produced in almost actual size without exaggeration. Thus, the measured height of the trees turned out to be appropriate as baseline data for reproduction of the vegetation landscape. Third, through the Rubber Sheeting Transformation of , it was possible to make a ground plan for the planting of on the current digital topographic map. In particular, as the transformed area of was departmentalized and control points were added, the precision of transformation improved. It was possible to grasp the changed position of planting as well as the change in planting density through a ground plan of planting of . Lastly, it was possible to produce a three-dimensional vegetation landscape model by using the information of the shape of the trees and the ground plan for the planting of . Based on the three-dimensional model, it was easy to examine the characteristics of the three-dimensional view of the current vegetation via the view axis, skyline, and openness to and cover from the adjacent regions at the level of the eyes. This study is differentiated from others in that it verified the realism of and suggested the possibility of ascertaining the original form of the vegetation landscape described in the painting.

The Search for Study on the Construction Process and Changes in the Landscape Plants of the Pasanseodang ('파산서당'의 영건과정과 조경식물 변화상 탐색)

  • Joo, Been;Choi, Hayoung;Shin, Sangsup
    • Korean Journal of Heritage: History & Science
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    • v.51 no.1
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    • pp.48-65
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    • 2018
  • The authors of this paper aim to make a record of the construction process, its symbolic meaning, and the changes in the status of the landscape plants at the Pasanseodang according to the Report on the Pasanseodang written by Park Gyu-hyun in 1874. First, the construction of Samgahun Pavilion, which is located in Myo-ri, Habin-myun, Dalsung-gun, Daegu, took about 90 years and spanned the lifetimes of Park Sungsoo, an 11th-generation descendant of Park Paengnyun (1417~1456) through to Park Kyuhyun, a 14th-generation descendant. It was called the shape of dragon, with its head facing the tail (回龍顧尾形), in feng shui. Second, the village of Pahwoe was founded in 1769, the 45th year of the reign of King Yeongjo, by Park Sungsoo for the purpose of socializing with his friends at his thatched home, and was named after his own courtesy name (Samgahun). Park Kwangseok, the second son of Park Sungsoo, built the sarangchae in 1826 and the anchae in 1869 after his marriage (in 1783). Then, Park Kyuhyun, the grandson of Park Kwangseok, built the pond and planted it with lotus flowers, and built the Hayeopjeong in 1874. The Pasanseodang, as the precursor of the Hayeopjeong, may be related with the name of the hillside region behind Samgahun. Third, a quadrangular-shaped pond with a length of 21m and a width of 15m was also built and planted with lotus flowers. In the center of the pond is a small round island that reflects the world view of the Chosun dynasty, i.e. that the sky is round and the landmass is quadrangular. Meanwhile, the name of the Hayeopjeon reflects the value system of aristocrats who lived a life of leisure and artistic indulgence. They called the eastern room "Yeeyeonhun" (怡燕軒) and the western room "Mongyangjae" (蒙養齋), names which embody their wishes for a good life as a member of the nobility and a bright future for one's descendants. Fourth, in Confucian terms, the authors infer the points of view reflected in the kinds of trees that were planted according to Confucian norms (pine tree, lotus, bamboo), the living philosophy of sustainability (willow), the ideology of seclusion and the search for peace of mind (bamboo), and relief efforts for the poor and a life of practicality (chestnut, oak, wild walnut, lacquer). The authors assert that this way of planting trees was a highly effective design feature of landscape architecture that drew on the locational and symbolic significance of the Seodang. Fifth, the majority of the trees that were initially planted withered and were replaced with different species, except for the locust and lotus, at this point. Nevertheless, a review of the process of construction, symbolic meaning, and original architectural landscape of the Samgahun is of value in demonstrating the extended symbolic meaning of their descendants in terms of the practical loss of the function of the Seodang, the values of Feng Sui (red in the east, white in the west, based on the principles of Feng Sui), the function of repelling evils spirits (kalopanax, trifoliate orange), aesthetic and practical values (sweetbrier, apricot, pear, peach, and oriental oak trees), and the prosperity of the family and the timeless value of honest poverty (silk, crape myrtle, and yew trees).

Investigation on the Actual State of Grassland in Republic of Korea (국내 초지보유농가의 초지실태 연구)

  • Lee, Bae Hun;Kim, Ji Yung;Sung, Kyung Il;Kim, Byong Wan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.2
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    • pp.89-96
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    • 2019
  • This study was conducted to investigate 106 grassland farms in six provinces including Chungcheongbuk-do, Gangwon-do, Gyeonggi-do, Gyeongsangbuk-do, Gyeongsangnam-do, Jeju-do, and Jeollanam-do to present their problems and causes by surveying the actual state of grassland farms during three years(2014~2016). The grassland survey was divided into three categories; used as the perennial grasses and annual forage crops (Complied with Grassland Act), not used as the perennial grasses and annual forage crops (Not complied with Grassland Act) and failed to meet the farmer and some items are missing or inaccurate (Insufficient contents). Among the surveyed grassland farm, 68 farms (64.2 %) were complied with Grassland Act but 30 farms (28.3 %) were not complied with Grassland Act. Especially, the 8 farms (26.7 %) not complied with Grassland Act used the grassland as other purposes such as tree growing, golf club and swine farm etc.. Therefore, strict on-site investigation by local governments is required to prevent the grassland from being used by illegal purposes. And there's a strong likelihood that 5 farms (62.5 %) avoided the survey violate the positive law. Grassland grades used by the local administrative agencies were not influenced by the factors affecting the yield (existence and non-existence of overseeding and fertilization by grassland grade, soil pH and organic matter content). This results suggest that there is a fundamental problem on the current grassland grade system based on the yield and the irregular time of investigation and lack of on-site investigation are another causes for inaccurate grassland grade. Therefore, the new method evaluating grassland grades which is not based on yield and the thorough on-site investigation by local administrative agencies are necessary when the grassland grade is evaluated.

Growth Characteristics and Visible Injury of Container Seedling of Pinus densiflora by Fertilization Level (시비수준별 소나무 용기묘의 생장 특성 및 가시적 피해)

  • Cha, Young Geun;Choi, Kyu Seong;Song, Ki Seon;Gu, Da-Eun;Lee, Ha-Na;Sung, Hwan In;Kim, Jong Jin
    • Journal of Bio-Environment Control
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    • v.28 no.1
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    • pp.66-77
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    • 2019
  • The present study investigated pine trees, which forms a major plantation species in Korea, with the objective of improving the survival rate of pine trees after planting. Growth responses and characteristics were assessed by controlling the level of fertilizer application, which is a basic controlling the growth of pine seedlings, to identify the optimal fertilization treatment. Pine tree seedlings were grown in 104 containers and were examined 8 weeks after planting. Stem height and were measured at 4-week intervals. In terms of fertilization treatment for 1-0 pine seedlings, the treatment group with gradually-increasing fertilizer concentration ($500{\rightarrow}1000{\rightarrow}1000{\rightarrow}1000mg{\cdot}L^{-1}$) had the biggest increase in stem height and diameter at the root. The survey results indicated that the increased concentration treatment group and the gradually-increasing concentration treatment group had more growth compared with that in the fixed concentration treatment group. The gradually-increasing concentration treatment group ($500{\rightarrow}1000{\rightarrow}1000{\rightarrow}1000mg{\cdot}L^{-1}$) had the highest total dry matter production. Nine weeks after fertilization, the tips of the pine leaves turned yellow in the fixed concentration treatment group ($3000mg{\cdot}L^{-1}$). The same phenomenon was observed in the treatment group in which the concentration was increased to $2000mg{\cdot}L^{-1}$, and in the gradually-increasing concentration treatment group, when the concentration was raised up to $2000mg{\cdot}L^{-1}$. We concluded that the optimal fertilization conditions for producing healthy pine 1-0 seedlings involve fertilizing once a week with Multifeed 19 at $500mg{\cdot}L^{-1}$ during the seedling period, Multifeed 19 at $1000mg{\cdot}L^{-1}$ during the rapid growth period, and Multifeed 32 at $1000mg{\cdot}L^{-1}$ during the maturation period.

Evaluation of Standing Tree Characteristics by Development of the Criteria on Grading Hardwood Quality for Oaks Forests in Central Region of Korea (활엽수 입목형질등급 기준 개발을 통한 중부지역 참나무림의 입목특성 평가)

  • Lee, Young Geun;Lee, Sang Tae;Chung, Sang Hoon
    • Journal of Korean Society of Forest Science
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    • v.107 no.4
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    • pp.344-350
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    • 2018
  • This study was carried out to improve the forest management method considering the use of high value added timber in the natural broadleaf forests. For this purpose, the criteria for evaluating the quality grade of standing trees were established and applied to the oak stand in the central region of Korea. The evaluation factors of the grade were bending of stem, branch, stem damage, and other defects. If the logs are divided into 2.1 m units and three logs up to 6.3 m are available, they are classified as Grade I (G-I). If two logs are available, they are classified as Grade II (G-II), If only one log is available, it is classified as Grade III (G-III). When any log is not available as timber, it is classified as Grade IV (G-IV). As a result of applying the grade to the oak stand, G-I was 6.7 %, G-II was 28.0 %, G-III was 38.3 %, and G-IV was 27.0 %. The ratio of standing trees by oak species of higher than G-III was 88.2 % for Quercus acutissima, 88.1 % for Q. variabilis, 83.5 % for Q. serrata, 56.3 % for Q. aliena, and 50.3 % for Q. mongolica, respectively. The G-IV ratio for Q. variabilis and Q. mongolica tended to decrease with increasing diameter at breast height. The order of major defect affecting the grading level was bending of stem > branch > stem damage > other defects. Considering the grade level and oak species distribution, it was concluded possible to produce high quality hardwood timber when we concentrate forest tending techniques on Q. acutissima and Q. variabilis stand. In order to improve the accuracy of grading, it is necessary to continuous complement through the monitoring research for evaluation factors.

Study on the Estimation between CO2 Flux in Tree and Atmosphere (산림-대기 간 이산화탄소 교환량 산정 연구)

  • Kim, So Young;Park, Hyun Ju;Hong, You Deog;Han, Jin Seok;Son, Jung Seok;Park, Ji Hoon
    • Journal of Climate Change Research
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    • v.4 no.4
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    • pp.305-316
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    • 2013
  • The purpose of this study is to monitor the flux of $CO_2$ between the atmosphere and forest. The main research activities are conducted at Taehwa Mt. (Gangju, Kyeonggi, Korea), The Taehwa site is located 60 km north-east from the center of Seoul Metropolitan Area. The TRF flux tower is in the middle of a Korean Pine (Pinus koraiensis) plantation ($400m{\times}400m$), surrounded by a mixed forest. Eddy covariance method was used for $CO_2$ flux above the forest. $CO_2$ flux was measured from September to November 2011 and March to June 2012. It was found that $CO_2$ fluxes were observed between the atmosphere and forest. $CO_2$ was absorbed by plants through photosynthesis during the day and released during the night. $CO_2$ flux were respectively observed 0.7~0.2, 0.5~0.1, $0.3{\sim}0.1mgCO_2m^{-2}s^{-1}$ in Septem- ber, October, November 2011. $CO_2$ fluxes released by plants in the early morning(00:00~07:30h) and evening(18:00~24:00h) time. But $CO_2$ was absorbed by plants through photosynthesis in the day time(08:00~7:30h).

Mass-rearing Techniques of Anastatus orientalis (Hymenoptera: Eupelmidae), as the Egg-parasitoid of Lycorma delicatula (Hemiptera: Fulgoridae): An Using Method of Antheraea pernyi (Lepidoptera: Saturniidae) and L. delicatula Eggs in Laboratory (꽃매미 알 기생천적인 꽃매미벼룩좀벌의 대량사육기술: 산누에나방과 꽃매미 알 활용 방법)

  • Seo, Meeja;Kim, Jeong Hwan;Seo, Bo Yoon;Park, Changgyu;Choi, Byeong Ryeol;Kim, Kwang Ho;Ji, Chang Woo;Cho, Jum Rae
    • Korean journal of applied entomology
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    • v.57 no.4
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    • pp.243-251
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    • 2018
  • Eggs, immature eggs, and pupae of 8 different insects (Halyomopha halys, Riptortus pedestris, Lymantria dispar, Antheraea yamamai, Verlarifictorus spp, Antheraea pernyi, and Musca domestica) including Lycorma delicatura were used to select the alternative host for laboratory mass rearing of A. orientalis. Except L. delicatula's eggs and immature eggs of A. pernyi, other 7 tested insects were not parasitized by A. orientalis. A. pernyi was reared with oak tree leaves and its cocoons were harvested on mid-July and early October. On 4 or 5 days after emergence, only female adults showing swollen abdomen were collected and stored at $1{\sim}5^{\circ}C$. We could get 150~200 eggs per one female by dissecting the female's abdomen. For examining the possibility for laboratory mass rearing of A. orientalis with A. pernyi's immature eggs, developmental periods from egg to pupa between the two different hosts were compared. Developmental periods were 36.1 days on immature eggs of A. pernyi and 36.8 days on an original host's eggs, respectively. The number of parasitized eggs by A. orientalis' female for 24 h was 3.4 on immature eggs of A. pernyi and 4.2 on an original host's eggs, respectively. However, there were no significant statistical differences in developmental period and parasitization between the two hosts. By supplying honeyed water to newly emerged female parasitoids, it was able to maximize their longevities up to 64.3 days after emergence. Therefore, our results support potential for laboratory mass-rearing of A. orientalis using A. pernyi's immature eggs as an alternative host.