• Journal of the Institute of Electronics Engineers of Korea SP
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• v.44 no.5
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• pp.35-44
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• 2007

Since the omnidirectional camera system with a very large field of view could take many information about environment scene from few images, various researches for calibration and 3D reconstruction using omnidirectional image have been presented actively. Most of line segments of man-made objects we projected to the contours by using the omnidirectional camera model. Therefore, the corresponding contours among images sequences would be useful for computing the camera transformations including rotation and translation. This paper presents a novel two step minimization method to estimate the extrinsic parameters of the camera from the corresponding contours. In the first step, coarse camera parameters are estimated by minimizing an angular error function between epipolar planes and back-projected vectors from each corresponding point. Then we can compute the final parameters minimizing a distance error of the projected contours and the actual contours. Simulation results on the synthetic and real images demonstrated that our algorithm can achieve precise contour matching and camera motion estimation.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.9 no.8
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• pp.1709-1715
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• 2005

This paper describes the development of 3-D web graphic library for dynamic web graphic design. The 3-D web graphic library developed in this per supports creation of 3-D objects like cube and sphere objects, elimination of hidden line and surface, and the shading of diffuse and specular reflections. It provides, in drawing, perspective projection of an object depth first sort of multiple objects, and wire frame and solid models. It also supports texture mapping function for realistic and dynamic web application in application software. Each created 3-D object gives functions for the scaling, translation, and rotation of itself. It can be used for the development of dynamic web application software and the advertisement of information for business and tourism as a 3-D web graphic library engine. It is written in 'Java' language and runs on web browsers with Java virtual machine without any dependancy of client computer system.

• Journal of KIISE:Computing Practices and Letters
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• v.11 no.2
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• pp.180-191
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• 2005

The rapid growth of mobile communication technology has provided the expansion of mobile internet services, particularly mobile commerce takes much weight among them. Even though current mobile commerce service has serious problems which check its development, such as limited contents, expensive charge system and hardware restriction of mobile device, it is strongly expected as one of the next generation Internet services. In this paper, we summarize the problems like above and provide some total solution to meet them as follows: a function for automatic gathering of product information on online Internet and automatic translation it to data for mobile commerce, a middlelet application which provides functions for product search and order on the mobile device through off-line processing, and a function of user adaptive recommendation. We have actually designed and implemented the proposed system and verified the functions and effectiveness of the system.

• Korean Journal of Medicinal Crop Science
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• v.15 no.1
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• pp.46-50
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• 2007

Transformed hairy roots were induced from in vitro grown plantlets of Trichosanthes kirilowii by infection with Agrobacterium rhizogenes strain ATCC15834. Transformed hairy roots exhibited active growth with high branching of roots on plant growth regulators-free medium. Cloned line (TR-03) of hairy root was tested for its growth and extracellular protein accumulation in medium under various culture conditions. Among the culture media tested, a full-strength MS medium had a pronounced effect on root biomass and extracelluar protein accumulation in medium. The maximum root biomass (2.4 g DRW/flask) and extracellular total protein contents $(28.3ug/m\ell)$ in medium was obtained at inoculum size of 2 g (FRW) and in MS medium supplemented with 4% sucrose. In addition, the optimal shaking speed for root growth and extracellular protein accumulation in medium were 100 rpm. The total extracellualr protein concentration reached a maximum of $28.3ug/m\ell$ at 4 weeks and decreased thereafter. Protein translation inhibitory activity was observed in culture broths and reached levels of 21.3 unit. These studies demonstrate that the transformed hairy roots can be utilized for the in vitro production of ribosome-inactivating proteins.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.6
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• pp.1095-1100
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• 2008

In a process of insect foot recognition, footprint segments as basic areas for recognition need to be extracted from scanned insect footprints and appropriate features should be found from the footprint segments in order to discriminate kinds of insects, because the characteristics of the features are important to classify insects. In this paper, we propose methods for automatic footprint segmentation and feature extraction. We use a Trace transform method in order to find out appropriate features from the extracted segments by the above methods. The Trace transform method builds a new type of data structure from the segmented images by functions using parallel trace lines and the new type of data structure has characteristics invariant to translation, rotation and reflection of images. This data structure is converted to Triple features by Diametric and Circus functions, and the Triple features are used for discriminating patterns of insect footprints. In this paper, we show that the Triple features found by the proposed methods are enough distinguishable and appropriate for classifying kinds of insects.

• Journal of Life Science
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• v.21 no.7
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• pp.1046-1051
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• 2011

Diallyl disulfide (DADS), the most prevalent oil-soluble organosulfur compound in garlic, is known to have diverse biological activities, including anticarcinogenic, antiatherosclerotic, antiinflammatory, and antioxidant actions. In this study, we investigated the effect of DADS on the expression of heme oxygenase-1 (HO-1) in human liver hepatoma cell line HepG2. Treatment of HepG2 cells by DADS evoked a dose-dependent growth inhibition without significant toxicity to the cells, and also induced the expression of transcription factor Nrf2. However, DADS did not have any enhancing effect on transcription and translation of HO-1 expression in HepG2 cells. In addition, DADS efficiently blocked protein synthesis of HO-1 in HepG2 cells stimulated by CoPP or hemin. But, DADS did not decrease the content of transcripts of HO-1 gene stimulated by CoPP, with accumulation of Nrf2 and small Maf in the nucleus. Based on these results, we conclude that DADS inhibits HO-1 expression by modulation of translational level of CoPP or hemin-induced HO-1 expression in HepG2 cells.

• Animal Bioscience
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• v.36 no.6
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• pp.851-860
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• 2023

Objective: This study aims to evaluate the target genes of gga-miR-20a-5p and the regulated immune responses in the chicken macrophage cell line, HD11, by the exosome-mediated delivery of miR-20a-5p. Methods: Exosomes were purified from the chicken macrophage cell line HD11. Then, mimic gga-miR-20p or negative control miRNA were internalized into HD11 exosomes. HD11 cells were transfected with gga-miR-20a-5p or negative control miRNA containing exosomes. After 44 h of transfection, cells were incubated with or without 5 ㎍/mL poly(I:C) for 4 h. Then, expression of target genes and cytokines was evaluated by quantitative realtime polymerase chain reaction. Results: Using a luciferase reporter assay, we identified that gga-miR-20a-5p directly targeted interferon gamma receptor 2 (IFNGR2), mitogen-activated protein kinase 1 (MAPK1), mitogen-activated protein kinase kinase kinase 5 (MAP3K5), and mitogen-activated protein kinase kinase kinase 14 (MAP3K14). Moreover, the exosome-mediated delivery of gga-miR-20a-5p successfully repressed the expression of IFNGR2, MAPK1, MAP3K5, and MAP3K14 in HD11 cells. The expressions of interferon-stimulated genes (MX dynamin like GTPase 1 [MX1], eukaryotic translation initiation factor 2A [EIF2A], and oligoadenylate synthase-like [OASL]) and proinflammatory cytokines (interferon-gamma [IFNG], interleukin-1 beta [IL1B], and tumor necrosis factor-alpha [TNFA]) were also downregulated by exosomal miR-20a-5p. In addition, the proliferation of HD11 cells was increased by exosomal miR-20a-5p. Conclusion: The exosome-mediated delivery of gga-miR-20a-5p regulated immune responses by controlling the MAPK and apoptotic signaling pathways. Furthermore, we expected that exosomal miR-20a-5p could maintain immune homeostasis against highly pathogenic avian influenza virus H5N1 infection by regulating the expression of proinflammatory cytokines and cell death.

• Tuberculosis and Respiratory Diseases
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• v.44 no.3
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• pp.547-558
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• 1997

Background : Tumor necrosis factor(TNF) has been considered as an important candidate for cancer gene therapy based on its potent anti-tumor activity. However, since the efficiency of current techniques of gene transfer is not satisfactory, the majorities of current protocols is aiming the in vitro gene transfer to cancer cells and re-introducing genetically modified cancer cells to hoot. In previous study, it was shown that TNF-sensitive cancer cells transfected with TNF-$\alpha$ cDNA would become highly resistant to TNF. Understanding the mechanisms of TNF-resistance in TNF-$\alpha$ gene transfected cancer cells would be an important step for improving the efficacy of cancer gene therapy as well as for better understandings of tumor biology. This study was designed to evaluate the role of new protective protein synthesis in the acquired resistance to TNF of TNF-$\alpha$ gene transfected cancer cells. Method : We transfected TNF-$\alpha$ c-DNA to WEHI164, a murine fibrosarcoma cell line, using retroviral vector(pLT12SN(TNF)) and confirm the expression of TNF with PCR, ELISA, MIT assay. Then we determined the TNF resistance of TNF gene transfected cells(WEHI164-TNF) and the changes of TNF sensitivities after treatments with actinomycin D(transcription inhibitor) and cycloheximide ( translation inhibitor). Results : WEHI164 which was sensitive to TNF became resistant to TNF after being transfected with TNF-$\alpha$ gene and the resistance to TNF was partially reversed after treatment with actinomycin D, but not with cycloheximide. Conclusion : The acquired resistance to TNF after TNF-$\alpha$ gene transfection may be associated with synthesis of some protective proteins.

• Progress in Medical Physics
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• v.12 no.2
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• pp.177-184
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• 2001

The goal of radiation treatment is to deliver a prescribed radiation dose to the target volume accurately while minimizing dose to normal tissues. Due to inaccurate placement of field and shielding block and patient's movement, there could be displacement errors between the planed and treatment regions. In order to verify the location of radiation treatment, we in this study developed the registration algorithm of the x-ray simulator images and portal images and quantified the inaccuracy in terms of shift, scale and rotation. The algorithm for registration of pairs of radiation fields consists of the alignment of pairs of radiation images by points matching and field displacement analysis by field boundary matching. In the first step, paired surface landmarks are matched to calculate the transformation parameters (scale, rotation and shift) using the corresponding line pairs which are created by connecting two landmarks of each image. In the next step, portal field boundary is extracted and then the two field boundaries are matched by the $\rho$-$\theta$ technique. Applying the phantom portal images, detection errors were calculated to be less than 2mm in translation, 1$^{\circ}$ in rotation and 1% in scale. In conclusion, we quantitatively analyzed the displacement error of x-ray simulator images and portal images. The present results could contribute to the study of the radiation treatment verification.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.37 no.3
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• pp.70-78
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• 2000

In this study, a new method for canceling MRI artifacts through the motion translation of image plane is presented Breathing often makes problems in a clinical diagnosis. Assuming that the head moves up and down due to breathing, rigid translational motions in only y(phase encoding axis) direction are treated Unlike the conventional Iterative phase retrieval algorithm, this method is based on the MRI imaging process and analyzing of Image property A new constraint condition with which the motion component and the true image component in the MRI signal can be separated by a simple algebraic operation is extracted After the x(read out) directional Fourier transformation of MRI signal is done, the y(phase encoding) directional spectrum phasing value is Just an algebraic sum of the Image component and the motion component Meanwhile, as It is known that the density of subcutaneous fat area is almost uniform in the head tomographs, the density distribution along a y directional line on this fat area is regarded as symmetric shape If the density function is symmetric, then the phase of spectrum changes linearly with the position Hence, the departure component from the linear function can be separated as the motion component Based on this constrant condition, the new method of artifact cancellation is presented Finally, the effectiveness of this algorithm IS shown by using a phantom with simulated motions.