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http://dx.doi.org/10.5352/JLS.2011.21.7.1046

Effect of Diallyl Disulfide on Heme Oxygenase-1 Expression in Human Hepatoma Cell Line HepG2  

Kim, Kang-Mi (Department of Microbiology & Immunology, Pusan National University School of Medicine)
Lee, Sang-Kwon (Cardiovascular Center, Pusan National University Yangsan Hospital)
Park, Young-Chul (Department of Microbiology & Immunology, Pusan National University School of Medicine)
Publication Information
Journal of Life Science / v.21, no.7, 2011 , pp. 1046-1051 More about this Journal
Abstract
Diallyl disulfide (DADS), the most prevalent oil-soluble organosulfur compound in garlic, is known to have diverse biological activities, including anticarcinogenic, antiatherosclerotic, antiinflammatory, and antioxidant actions. In this study, we investigated the effect of DADS on the expression of heme oxygenase-1 (HO-1) in human liver hepatoma cell line HepG2. Treatment of HepG2 cells by DADS evoked a dose-dependent growth inhibition without significant toxicity to the cells, and also induced the expression of transcription factor Nrf2. However, DADS did not have any enhancing effect on transcription and translation of HO-1 expression in HepG2 cells. In addition, DADS efficiently blocked protein synthesis of HO-1 in HepG2 cells stimulated by CoPP or hemin. But, DADS did not decrease the content of transcripts of HO-1 gene stimulated by CoPP, with accumulation of Nrf2 and small Maf in the nucleus. Based on these results, we conclude that DADS inhibits HO-1 expression by modulation of translational level of CoPP or hemin-induced HO-1 expression in HepG2 cells.
Keywords
Diallyl disulfide (DADS); heme oxygenase-1 (HO-1); Nrf2; small Maf; HepG2 cells;
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