• The Journal of Natural Sciences
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• v.4
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• pp.103-142
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• 1991

These studies were carried out to select somatic hybrid using selectable marker genes of Nicotiana glauca transformed by NPTII gene and Solanum tuberosum transformed by T- DNA, and to study characteristics of transformant. The results are summarized as follows. 1. Crown gall tumors and hairy roots were formed on potato tuber disc infected by A. tumefaciens Ach5 and A. rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. 2. Callus formation from hairy root was prompted on the medium containing 2, 4 D 2mg/I with casein hydrolysate lg/l. 3. The survival ratio of crown gall tumor callus derived from potato increased on the medium containing the activated charcoal 0. 5-2. 0mg/I because of the preventions on the other hand, hairy roots were necrosis on the same medium. 4. Callus derived from hairy root were excellently grown for a short time by suspension culture on liquid medium containing 2, 4-D 2mg/I and casein hydrolysate lg/l. 5. The binary vector pGA643 was mobilized from E. coli MC1000 into wild type Agrobacteriurn tumefaciens Ach5, A. tumefaciens $A_4T$ and disarmed A. tuniefaciens LBA4404 using a triparental mating method with E. ccli HB1O1/pRK2013. Transconjugants were obtained on the minimal media containing tetracycline and kanamycin. pGA643 vectors were confirmed by electrophoresis on 0.7% agarose gel. 6. Kanamycin resistant calli were selected on the media supplemented with 2, 4-D 0.5mg/1 and kanamycin $100\mug$/ml after co- cultivating with tobacco stem explants and A. tumefaciens LBA4404/pGA643, and selected calli propagated on the same medium. 7. The multiple shoots were regenerated from kanamycin resistant calli on the MS medium containing BA 2mg/l. 8. Leaf segments of transformed shoot were able to grow vigorusly on the medium supplemented with high concentration of kanamycin $1000\mug$/ml. 9. Kanamycin resistant shoots were rooting and elongated on medium containing kanamycin $100\mug$/ml, but normal shoot were not. 10. For the production of protoplast from potato calli transformed by T-DNA and mesophyll tissue transformed by NPTII gene, the former was isolated in the enzyme mixture of 2.0% celluase Onozuka R-10, 1.0% dricelase, 1.0% macerozyme. and 0.5M mannitol, the latter was isolated in the enzyme mixture 1.0% Celluase Onozuka R-10, 0.3% macerozyme, and 0.7M mannitol. 11. The optimal concentrationn of mannitol in the enzyme mixture for high protoplast yield was 0.8M at both transformed tobacco mesophyll and potato callus. The viabilities of protoplast were shown above 90%, respectively. 12. Both tobacco mesophyll and potato callus protoplasts were fused by using PEG solution. Cell walls were regenerated on hormone free media supplemented with kanamycin after 5 days, and colonies were observed after 4 weeks culture.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2006.11a
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• pp.208-211
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• 2006

This study was to find out contemporary planning trends of rural houses in Na-po Munhwa village by analyzing their floor plan shapes and space relations & elements. There were built 240 houses in that village, but it were possible to collect housing data, floor plans & general building records of 120 houses, from house register. By analyzing those data, findings were as follows : 1) Floor plan shapes of rural houses had a tendency to 'ㅋ' shape type than simple box or transformed box types, and Room-Living-Room arrangement of centered living were very strong trends in those plans. 2) Public spaces, L.D.K

• Journal of the Korean Institute of Rural Architecture
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• v.13 no.1
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• pp.1-8
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• 2011

This aim of the study is to investigate the plan and elevation types of existing way stations built in the early 20th century Korea, focused on Choong-ang, Donghae-nambu, and Kyung-jeon line of the railroad tracklines. The plans and elevations are drawn up and analyzed based on literature data and aerial photographs through the survey. These way stations are classified into various types by room layout and front elevation. The basic plan types are composed of waiting room, station service room, and administration. The station service room has a train operation room in the projecting part of corner. This room has windows on every side to see in and out of train. There are the enlarged, extended waiting room types, and linear types in the other plan types. The waiting room is lied on the right side, and has a gable roof in elevation. The elevation types are classified with side and centralized, and so on by the placement and shape of gable roofs. The basic elevation types have gable roofs in sides lied on the waiting room, and these types are transformed into centralized types by extension of waiting room The others are the linear types without gable roofs and the unusual roof types in appearance.

• Journal of the Korean Chemical Society
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• v.59 no.3
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• pp.238-245
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• 2015

The crystallization and morphology change of amorphous titanias by hydrothermal treatment have been investigated. The amorphous titanias were prepared by pure water hydrolysis of two different precursors, titanium tetraisopropoxide (TTIP) and TTIP modified with acetic acid (HOAc) and characterized prior to hydrothermal treatment. In order to avoid complicate situation, the hydrothermal treatment was performed in a single solvent water with and without strong acids at various temperatures. The effects of strong acid, temperature and time were systematically investigated on the transformation of amorphous titania to crystalline TiO₂ under simple hydrothermal condition. Without strong acid the titanias were transformed into only anatase phase nanoparticle regardless of precursor type, temperature and time herein used (up to 250 ℃ and 48 hours). The treatment temperature and time effected only on the crystalline size, not on the crystal phase et al. However, it was clearly revealed that the strong acids such as HNO₃ and HCl catalyzed the formation of rutile phase depending on temperature. HCl was slightly better than HNO₃ in this catalytic activity. The morphology of rutile TiO₂ formed was also a little affected by the type of acid. The precursor modifier, HOAc slightly reduced the catalytic activity of the strong acids in rutile phase formation.

• Journal of architectural history
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• v.29 no.3
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• pp.51-66
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• 2020

Paldalmun gate was built in 1794 as the southern gate of Suwon Hwaseong. The signboard's paintworks of Paldalmun gate was applied to the signboard of the state-run building in the 18th century. The signboard of Paldalmun gate was repainted along with the building when repaired in 1969. In that time, the signboard's paintworks during the reign of Jeongjo era was transformed. The purpose of this study is to restore the image of Jeongjo era paintworks on the Paldalmun's signboard, which has been modified since the 1960s due to modification. For the purpose of the examination, we examined the traces of paintworks remaining on the signboard of Paldalmun gate and examined several state-run signboards decorated with 'Yukhwamoon' paintings similar as the signboard of Paldalmun gate, which made around the 18th century. Through the above-mentioned researches, typical color features were identified in the decorated with 'Yukhwamoon' paintings on signboard in the 18th and 19th centuries. In addition, the type of pigment used in the signboard's paintwork of Paldalmun gate was verified through the 『Hwaseong Seongyeok Uigwe(華城城役儀軌)』 and the 『Han-gul Jeongri Uigwe(한글整理儀軌)』, while analyzing the characteristics of age-related deterioration according to the type of paintworks in various traces of pigment coatings remaining on the signboard of Paldalmun gate.

• Nuclear Engineering and Technology
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• v.35 no.5
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• pp.471-481
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• 2003

In order to evaluate the microstructural characteristics of irradiated fuel claddings (Zircaloy-4), two irradiated specimens having different burnups (18 GWD/MTU and 42 GWD/MTU) were prepared from the G23-M4 fuel rods, which were loaded in Kori Unit 1 for 4 fuel cycles. The oxide thickness, hydride morphology and hardness change were characterized by an optical microscope and a micro-hardness tester after preparing the irradiated specimens in the PIE (Post Irradiation Examination) facilities. The dislocation loops and the amorphous transformation of precipitates induced by the neutron irradiation in the nuclear plant were also examined by a TEM. As the burnup increased from 18 GWD/MTU to 42 GWD/MTU, the oxide thickness increased from $6.0{\mu}m\;to\;25.3{\mu}m$ and the contents of hydrogen pick-up in the Zr matrix also greatly increased. In the comparison of the hardness of the unirradiated fuel cladding, the amount of hardness increase was nearly $9.3\%\;and\;24.2\%$ for the irradiated samples of 18 and 42 GWD/MTU, respectively. Both -type dislocation loops and -type dislocation components were observed simultaneously in the irradiated specimens and the densities of the dislocation was increased by increasing the burnup. The precipitates in both the irradiated specimens were amorphously transformed by the neutron irradiation and the trend of the amorphous transformation of the precipitates was enhanced at a higher burnup.

• Journal of Ecology and Environment
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• v.34 no.2
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• pp.237-249
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• 2011

Land-use changes due to the socio-economic environment influence landscape patterns and processes, which affect habitats and biodiversity. This study considers the effects of such land-use changes, particularly on the traditional rural "Maeul" forested landscape, by analyzing landscape structure and vegetation changes. Three study areas were examined that have seen their populations decrease and age over the last few decades. Five types of plant life-forms (Raunkier life-forms) were distinguished to investigate ecosystem function. Principle component analysis was used to understand vegetation dynamics and community characteristics based on a vegetation similarity index. Ordination analysis transformed species-coverage data was introduced to clarify vegetation dynamics. Landscape indices, such as area metrics, edge metrics, and shape metrics, showed that spatial heterogeneity has increased over time in all areas. Pinus densiflora was the main land-use plant type in all study areas but decreased over time, whereas Quercus spp. increased. Over a decade, P. densiflora communities shifted to deciduous oak and plantation. These findings indicate that the impact of human activities on the Maeul landscape is twofold. While forestry activities caused heavy disturbances, the abandonment of traditional human activities has led to natural succession. Furthermore, it can be concluded that the type and intensity of these human impacts on landscape heterogeneity relate differently to vegetation succession. This reflects the cause and consequence of patch dynamics. We discuss an approach for sustainable landscape planning and management of the Maeul landscape based on traditional management.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.4
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• pp.673-678
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• 1997

Stress wave propagations in an elastic half space to a normal point force of ramp type in time are analyzed. The governing equations are transformed by applying the Laplace and Hankel transforms with respect to time and radial distance. The inversion of Laplace transforms are performed by employing the Cagniard-de Hoop method, where the Rayleigh waves at surface are obtained by including the residue terms. The stress waves computed at the location very cose to the surface are shown to be almost identical to the surface waves obtained by the residue method except the Rayleigh wavefront. It is found that at the surface, the stresses are dominated by the Rayleigh waves, whose amplitudes increase linearly with time when time is very large. It is also found that in the interior part, the radial stress has a logarithmic singularity at the shear wavefront, while tangential stress shows no singularity.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.8 no.1
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• pp.74-80
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• 1999

Recently, manufacturing work has been transformed to small scale production form with various types to act up to user's expectation from mass production with a little items required in the past. Then FMS using NC type machinaries has been applied actively also in domestic manufacturing line to meet thus tendency, but there are many machining troubles occured during work process not be settled yet. Nowdays high efficiently has been required no less than high precision in grinding work for the improvement of productivity. In this study, to represent more advance FMS can be adapted to thus situation In-process type monitoring method using AE and Current sensors is suggested to investigate the machining condition in grinding process. As results form this experimental study, it is recognized well that grinding conditions and dressing point of in time cab be estimated effectively using monitoring method suggested. Furthermore, surface shape of grinding wheel on voluntary point of in time can be predicted indirectly through the observation and comparison of AE signal waveform obtained as performance of continuous dressing work.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.72-76
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• 2017

Detection of exon 19 deletion mutation in the epidermal growth factor receptor (EGFR) gene, which results in increased and sustained phosphorylation of EGFR, is important for diagnosis and treatment guidelines in non-small-cell lung cancer. Here, we have developed a simple and convenient detection system using the interaction between G-quadruplex and fluorophore thioflavin T (ThT) for discriminating EGFR exon 19 deletion mutant DNA from wild type without a label and quencher. In the presence of exon 19 deletion mutant DNA, the probe DNAs annealed to the target sequences were transformed into G-quadruplex structure. Subsequent intercalation of ThT into the G-quadruplex resulted in a light-up fluorescence signal, which reflects the amount of mutant DNA. Due to stark differences in fluorescence intensity between mutant and wild-type DNA, we suggest that the induced G-quadruplex structure in the probe DNA can report the presence of cancer-causing deletion mutant DNAs with high sensitivity.