• BMB Reports
• /
• v.28 no.3
• /
• pp.265-270
• /
• 1995

Acetolactate synthase (ALS, EC 4.1.3.18) is the first common enzyme in the biosynthesis of leucine, isoleucine, and valine. It is the target enzyme for several classes of herbicides, including the sulfonylureas, the imidazolinones, the triazolopyrimidines, the pyrimidyl-oxy-benzoates and the pyrimidyl-thio-benzens. The sulfonylurea-resistant ALS gene (SurB) from Nicotiana tabaccum [Lee et al. (1988) The EMBO J. 7, 1241-1248] was cloned into the bacterial expression plasmid pT7-7. The resulting recombinant plasmid pT7-ALS was used to transform an ALS-deficient Escherichia coli strain MF2000. MF2000 cells transformed with pT7-ALS grew in the absence of valine and isoleucine. ALS activities of 0.042 and 0.0002 ${\mu}mol/min/mg$ protein were observed in the crude extracts prepared from MF2000 cells transformed with plasmids pT7-ALS and pT7-7, respectively. In addition, the former crude extract containing mutant ALS was insensitive to inhibition by K11570, a new chemical class of herbicides. $IC_{50}$ values for K11570 were $0.13{\pm}0.01$ mM. For comparison, a plasmid pTATX containing the wild-type Arabidopsis thaliana ALS coding sequences was also expressed in MF2000. ALS activities of 0.037 ${\mu}mol/min/mg$ protein were observed, and the wild type ALS was sensitive to two different classes of herbicides, K11570 and ALLY, a sulfonylurea. $IC_{50}$ values for K11570 and ALLY were $0.63{\pm}0.07$ and $80{\pm}5.6$ nM, respectively. Thus, the results suggest that the sulfonylurea-resistant tobacco ALS was functionally expressed in the bacteria, and that K11570 herbicides bind to the regulatoty site of ALS enzymes.

• Journal of Applied Biological Chemistry
• /
• v.57 no.1
• /
• pp.41-45
• /
• 2014

In the present study, red ginseng extracts were fermented by Paecilomyces tenuipes and the protopanaxdiol-type ginsenosides in the extracts were bio-transformed to F2, Rg3, Rg5, Rk1, Rh2, and CK determined by a high-pressure liquid chromatography analysis. It indicates that P. tenuipes is a microorganism to biotransform protopanaxdiol-type ginsenosides to their less glucosidic metabolites. Other biotransformed metabolites during fermentation were also analyzed using a GC-MS and identified as 2-methyl-benzaldehyde, 4-vinyl-2-methylphenol, palmitic acid, and linoleic acid. Antiulcerogenic activity of the fermented red ginseng extract (FRGE) on gastric mucosal damage induced by 0.15 M HCl in ethanol in rats was evaluated. FRGE was shown to have a potent protective effect on gastritis with 60.5% of inhibition rate at the dose of 40 mg/kg when compared to 54.5% of the inhibition rate at the same dose for stillen, the currently used medicine for treating gastritis. Linoleic acid showed a strong inhibition on gastritis with 79.3% of inhibition rate at the dose of 40.0 mg/kg. FRGE exhibited a distinct anticancer activity including growth inhibition of the two human colon cancer cells HT29 and HCT116. HT29 cells were less susceptible to FRGE in comparison with HCT116 cells. Taken together, fungal fermentation of the red ginseng extract induced hydrolysis of some ginsenosides and FRGE exhibited potent antiulcerogenic and anticancer activities. These results refer to use FRGE as a new source for treating human diseases.

• Journal of KIISE:Computer Systems and Theory
• /
• v.36 no.4
• /
• pp.239-246
• /
• 2009

In this study, we analyze the mean waiting time on the nested open queueing network, where the population within each subnetwork is controlled by a semaphore queue. The queueing network can be transformed into a simpler queueing network in terms of customers waiting time. A major characteristic of this model is that the lower layer flow is halted by the state of higher layer. Since this type of queueing network does not have exact solutions for performance measure, the lower bound and upper bound on the mean waiting time are checked by comparing them with the mean waiting time in the transformed nested queueing network. Simulation estimates are obtained assuming Poisson arrivals and other phase-type arrival process, i.e., Erlang and hyper-exponential distributions. The bounds obtained can be applied to get more close approximation using the suitable approach.

• Smart Structures and Systems
• /
• v.14 no.5
• /
• pp.831-845
• /
• 2014

This paper proposed a discrete wavelet transform based method for time-varying physical parameter identification of shear type structures. The time-varying physical parameters are dispersed and expanded at multi-scale as profile and detail signal using discrete wavelet basis. To reduce the number of unknown quantity, the wavelet coefficients that reflect the detail signal are ignored by setting as zero value. Consequently, the time-varying parameter can be approximately estimated only using the scale coefficients that reflect the profile signal, and the identification task is transformed to an equivalent time-invariant scale coefficient estimation. The time-invariant scale coefficients can be simply estimated using regular least-squares methods, and then the original time-varying physical parameters can be reconstructed by using the identified time-invariant scale coefficients. To reduce the influence of the ill-posed problem of equation resolving caused by noise, the Tikhonov regularization method instead of regular least-squares method is used in the paper to estimate the scale coefficients. A two-story shear type frame structure with time-varying stiffness and damping are simulated to validate the effectiveness and accuracy of the proposed method. It is demonstrated that the identified time-varying stiffness is with a good accuracy, while the identified damping is sensitive to noise.

• Journal of Korea Multimedia Society
• /
• v.23 no.1
• /
• pp.74-84
• /
• 2020

In November 2018, virtual pop band K/DA, with the use of holograms and actual K-pop girl group members, performed its debut album titled "POP/STARS" at the opening ceremony of the League of Legends S8 final, hosted by Riot Games. The fan culture that exists for real singers adapts itself to virtual bands, and K/DA, consisting of virtual characters, encouraging the production of user created content (UCC). However, this form of UCC is different from the most common one, meaning that this type of band generates another way of consuming content. Therefore, this study examines how K/DA's digital characters are transformed and reproduced by UCC and how the original producers' content differs from them. Firstly, the development of this study considers the open characteristics of UCC, such as user type, created motivation, created process, content type and sharing method. In the second part, the virtual idol Hatsune Miku and virtual band Pentakill will be analysed to understand UCC. Thirdly, K/DA will be analyzed as a case study based on three factors: created motivation, content type and sharing method. In this manner, this study aims to explain how UCC for virtual bands reproduce and consume original content and expand their commercial value.

• BMB Reports
• /
• v.40 no.3
• /
• pp.333-340
• /
• 2007

Gene encoding cyclodextrin glycosyltransferase (CGTase), from thermotolerant Paenibacillus sp. T16 isolated from hot spring area in northern Thailand, was cloned and expressed in E. coli (JM109). The nucleotide sequences of both wild type and transformed CGTases consisted of 2139 bp open reading frame, 713 deduced amino acids residues with difference of 4 amino acid residues. The recombinant cells required 24 h culture time and a neutral pH for culture medium to produce compatible amount of CGTase compared to 72 h culture time and pH 10 for wild type. The recombinant and wild-type CGTases were purified by starch adsorption and phenyl sepharose column chromatography and characterized in parallel. Both enzymes showed molecular weight of 77 kDa and similar optimum pHs and temperatures with recombinant enzyme showing broader range. There were some significant difference in pH, temperature stability and kinetic parameters. The presence of high starch concentration resulted in higher thermostability in recombinant enzyme than the wild type. The recombinant enzyme was more stable at higher temperature and lower pH, with lower $K_m$ for coupling reaction using cellobiose and cyclodextrins as substrates.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2001.11a
• /
• pp.304-311
• /
• 2001

This research numerically analyzes the dynamic characteristics of a coupled journal and thrust hydrodynamic bearing due to its groove location which has the static load due to the weight of a rotor in the axial direction and the dynamic load due to its mass unbalance in the radial direction. The Reynolds equation is transformed to solve a plain member rotating type of journal bearing(PMRJ), a grooved member rotating type of journal bearing (GMRJ), a plain member rotating type of thrust bearing (PMRT) and a grooved member rotating type of thrust bearing (GMRT). FEM is used to solve the Reynolds equations in order to calculate the pressure distribution in a fluid film. Reaction forces and friction torque are obtained by integrating the pressure and shear stress along the fluid film, respectively. Dynamic behaviors, such as whirl radius or floating height of a rotor, are determined by solving its nonlinear equations of motion with the Runge-Kutta method. This research shows that the groove location affects the pressure distribution in the fluid film and consequently the dynamic performance of a HDD spindle system.

• Journal of the Korean Ceramic Society
• /
• v.33 no.9
• /
• pp.1064-1072
• /
• 1996

The synthetic conditions and characteristics of ZSM-5 type zeolites (ZSM-5/silicalite) for the preparation of the zeolite composite membranes for gas separation were investigated. ZSM-5 zeolites could be synthesized by the hydrothermal treatment of the mixture of colloidal silica sol aluminum nitrate sodium hydroxide and TPABr at a temperature range of 150-17$0^{\circ}C$ in the autoclave. Silicalties were done from the solution of water glass water and TPABr. Their crystalline structures transformed from orthorhombic to monoclinic from and their pore structures of three-dimensional channels were opened as TPABr filling channels was burned off at the calcination temperature of 50$0^{\circ}C$. The specific surface area of the calcined zeolite was about 360 m3/g and its surface property was hydrophobic. Crystal sizes of ZSM-5 and silicalite were 0.5-1.0${\mu}{\textrm}{m}$ and 8-10${\mu}{\textrm}{m}$ respectively having no change due to the calcination. In particular the shape and the size of the ZSM-5 type zeolite were sensitively varied with silica sources and concentrations of reaction solutions/sols.

• Geomechanics and Engineering
• /
• v.21 no.5
• /
• pp.461-469
• /
• 2020

The objective of this paper is to study the deformation in a homogeneous isotropic modified couple stress thermoelastic medium with and without energy dissipation and with two temperatures due to thermal source and mechanical force. Laplace and Fourier transform techniques are applied to obtain the solutions of the governing equations. The displacement components, stress components, conductive temperature and couple stress are obtained in the transformed domain. Isothermal boundary and insulated boundary conditions are used to investigate the problem.The effect of two temperature and GN theory of type-II and type-III has been depicted graphically on the various components. Numerical inversion technique has been used to obtain the solutions in the physical domain. Some special cases of interest are also deduced.

• Journal of the Korean Institute of Telematics and Electronics S
• /
• v.34S no.2
• /
• pp.33-42
• /
• 1997

In this paper, a video coding method in wavelet transformed multi-resolution image using variable block sized motion estimation and multi-codebook is proposed. In the propoed method, the accuracy of motion estimation is increased by using variable block matching algorithm based on edge type of blocks which estimation is increased by using variable block matching algoritm based on edge type of blocks which is classified accoridng to the magnitude of wavelet coefficients in vertical subband and horizontal subband of the highest layer. Also, we increased the flexibility of bit allocation and decreased vector quantization error for motion compensated error transmission by using importance of each subband. Some experimental results confirm that he proposed mothod has fine reconstructed images without blocking effect at low bit rate, and especially reconstructs edges well to which human eyes are sensitive.