• 제어로봇시스템학회논문지
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• 제9권12호
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• pp.1001-1008
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• 2003

A time-delay compensation method for vector control system is proposed that can compensate for voltage and current distortions resulting from a time delay in the overall system due to the low pass filter, hysteresis control inverter, microprocessor program computation time, and so on. The proposed scheme estimates the time delay using the difference between the Q-axis stator current command and the time-delayed actual Q-axis stator current in a synchronous reference frame, then compensates the time delay in the voltage and current using the angular displacement of a DQ transformation. Accordingly, the proposed scheme can accurately compensate for the time delay related to the overall system, thereby significantly improving the performance of the vector control system, as verified by simulation and experiment.

• 대한의생명과학회지
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• 제18권1호
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• pp.29-34
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• 2012

Streptomycetes are gram-positive filamentous bacteria that are well-known for producing a vast array of bioactive compounds, including more than 70 % of commercially important antibiotics. For the research about Streptomyces sp., the protoplast and electroporation transformation method have been the general techniques for the construction of transformants. However, these techniques have low efficiency and are time-consuming. Another option is intergenic conjugation, which is used for DNA transfer using methylation-deficient E. coli as a DNA donor to avoid the methylated-DNA-dependent restriction systems of actinomycetes. This conjugation method has been widely improved and applied to many other actinomycetes. In this research, an effective transformation procedure for the construction of expression vector by using gateway system was established to avoid limit of restriction enzyme site for cloning of target gene based on transconjugation by Escherichia coli ET12567/pUZ8002 with a pSET152 integration vector.

• Journal of Plant Biology
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• 제32권1호
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• pp.23-32
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• 1989

We have developed a plasmid vector, pKCH1, for the purpose of higher plant transformation. It contains the promoter region of cauliflower mosaic virus 35S transcript (P35s) and the terminator region of nopaline synthase gene (Tnos) with unique cloning sites, Bam HI and Xba I, between them. After inserting a foreing gene at the cloning sites, P35s-foreign gene-Tnos cassette can be recovered by using a restriction enzyme Hind III.

• 미생물학회지
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• 제39권3호
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• pp.128-134
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• 2003

Transformation-associated recombination (TAR) 클로닝법은 목적 유전자를 포함한 게놈 DNA와 그 유전자의 5' 또는 3' 말단 서열을 포함하고 있는 선형의 TAR vector를 동시에 출아효모의 spheroplast내로 co-penetration 시켜 상동부위에서 일어나는 재조합에 의해 환형의 Yeast Artification Chromosome(YAC)으로 분리되는 방법이다. 일반적으로 TAR 클로닝법에 의한 목적의 single-copy 유전자 분리 빈도는 전체 형질전환체의 0.01~1% 정도이다. 이러한 TAR 클로닝법을 개선하기 위하여 Tg.AC transgenic mouse를 모델계로 사용하여 유전자 분리에 대한 target hook 내의 GC content 가 미치는 영향을 조사하였다. 이러한 목적으로 한쪽에는 다양한 GC content(18~45%)를 지닌 transgene 특이적 hook을 포함하고 다른 한쪽은 B1 반복서열을 가지는 radial TAR vector를 사용하여 transgene 분리 빈도를 측정하였다. 그 결과 target hook의 GC content는 23% 이하의 경우, ~40%인 경우에 비해 두 배 정도 클로닝 빈도가 감소하였다. 따라서 TAR vector를 제작할 때, 유전자 분리에 이용되는 target hook의 GC content는 약 40% 일때 가장 적정한 것으로 나타났다. 또한 높은 target hook 내의 GC content(65%)위치분포에 의한 차이는 클로닝 빈도에 큰 영향을 미치지 않는 것으로 나타났다.

• 생명과학회지
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• 제16권3호
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• pp.365-368
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• 2006

본 실험에서는 식물의 유용유전자를 개발하여 그 발현양상을 확인하기 위하여 효모를 이용하면 그 발현양상을 비교적 빠르게 확인할 수 있는 미토콘드리아 형질전환 방법을 규명하였다. 또한 미토콘드리아(mt)에 관련된 유전자를 TPI promoter를 가진 plasmid에 재조합한 후 효모에 형질전환하여 mt에서 그 유전자의 특성이 발현 되는 것을 확인하였다. 따라서 본 연구의 결과로 mt에 관련된 유전자를 식물의 조직에 형질전환 하여 1개 이상의 유전자가 식물의 mt에 삽입되어 그 유전자의 특성이 발현되는데 이용되어 질수 있을 것이라 생각된다.

• 한국음향학회지
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• 제23권3호
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• pp.253-261
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• 2004

본 논문에서는 음성 신호가 지니고 있는 화자 의존적 특징 변수를 변환 시키는 음성 개성 변환 기법이 새롭게 제안되었다. 제안된 방법은 성도 전달 함수의 특성을 반영하는 켑스트럼 벡터와 여기 신호의 특성을 반영하는 피치 값을 변환 대상 변수로 삼았으며, 이들에 대한 변환 기법으로 다중 응답 분류 회귀 트리를 사용하였다. 다중 응답 분류 회귀 트리는 기존의 분류 회귀 트리를 다차원 확장시킨 형태로서, 반응값이 벡터 형태로 존재하는 분류 회귀 트리를 의미한다. 본 논문에서는 기존의 코드북 메핑 방법과 비교하여 제안된 기법의 성능을 평가하였으며, 분류 회귀 트리에 입력되는 관찰값을 다양하게 변화시켜 트리의 복잡도와 변환 성능을 정량적으로 분석하였다. 네 명의 화자를 이용한 음성 개성 변환 실험에서, 기존의 코드북 메핑과 비교하여 객관적으로 우수한 성능을 나타내었으며, 청취 테스트에서도 변환음이 목표로 하는 화자의 음성과 유사함을 관찰할 수 있었다.

• 한국초지조사료학회지
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• 제18권1호
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• pp.69-76
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• 1998

This study was conducted to construct of the transgenic plants wliich are resistant to oxidative stresses including ozone with B. mpestris cytosolic glutathione reductase cDNA using the binary vector system of Agrobacterium tumefaciens. The 1.8kb B. campestris cytosolic GR cDNA was subcloned into the unique Sma I site of the plant transformation vector pBKSI- I, downstream of the constitutive CaMV 35s promoter and upstream of the nos termination sequence, in place of the uidA (GUS) reporter gene. The resulting plant transformation vector, pBKS-GRI, was introduced into A. tumefaciens LBA4404 by two cycles of tkeze-thaw method. The B. nqus cotyledonary petioles were transformed by the Agrubaferium harboring pBKS-GRI. Transformed shoots were induced and selected on regeneration medium supplemented with kanarnycin. The shoot formation was increased remarkably by addition of Ag$NO_3$, in MS media. The transgenic plants were analyzed for the presence of the B. campestris GR gene by Southern blot analysis and it was confirmed that a foregin gene was stably integrated into the genomes of B. nqus plants.

• 한국정밀공학회지
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• 제17권10호
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• pp.141-146
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• 2000

We introduce the algorithms of 3-D position estimation using a laser sensor for automatic die remodeling. First, a vision sensor based on the optical triangulation was used to collect the range data of die surface. Second, line vector equations were constructed by the measured range data, and an analytic algorithm was proposed for recognizing the die location with these vector equations. This algorithm could make the transformation matrix without any specific corresponding points. To ascertain this algorithm, folded SUS plate was measured by the laser vision sensor attached to a 3-axis cartesian manipulator and the transformation matrix was calculated.

• Journal of Plant Biology
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• 제38권4호
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• pp.365-371
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• 1995

Binary vectors, pBI-ActR1, pBI-ActF1 and pBSH-ActR1, were constructed using pGA642, the replication origin of pTi12 and the rice actin promoter. The sizes of pBI-ActR1, pBI-ActF1 and pBSH-ActR1 were 12.9 kb, 13.2 kb and 11.95 kb, respectively. These vectors containing a rice actin promoter followed by a GUS structural gene could induce stronly the expression of GUS gene in transformed rice cells. Rice explants from 3-4 day old seedlings after germinatin were cocultured with A. tumefaceins harboring pBI-ActR1, pBI-ActF1 or pBSH-ActR1, and then GUS expression in the explants was assayed. Transformation of rice explants by these binary vectors was tissue-specific, such that the meristematic regions of shoot apex, root and hypocotyl were transformed by these binary vectors.

• 전자공학회논문지S
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• 제35S권4호
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• pp.118-127
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• 1998

In this paper, we propose a voice personality transformation method which makes one person's voice sound like another person's voice. In order to transform the voice personality, vocal tract transfer function is used as a transformation parameter. Comparing with previous methods, the proposed method can obtain high-quality transformed speech with low computational complexity. Conversion between the vocal tract transfer functions is implemented by a linear mapping based on soft clustering. In this process, mean LPC cepstrum coefficients and mean removed LPC cepstrum modeled by the low dimensional vector are used as transformation parameters. To evaluate the performance of the proposed method, mapping rules are generated from 61 Korean words uttered by two male and one female speakers. These rules are then applied to 9 sentences uttered by the same persons, and objective evaluation and subjective listening tests for the transformed speech are performed.