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http://dx.doi.org/10.5352/JLS.2006.16.3.365

Identification of Artificial Operon Gene Expression via Yeast Mitochondrial Transformation  

Kim Kyung-Min (Department of Environmental Horticulture, Sangju National University)
Sul Il-Whan (Department of Biotechnology, Daegu University of Foreign Studies)
Publication Information
Journal of Life Science / v.16, no.3, 2006 , pp. 365-368 More about this Journal
Abstract
Yeast mitochondrial transformation has been confirmed by cell death and CFP expression (CDF: cell death factor gene). Expression vector containing CDF and CFP driven by one TPI (Triose-phosphate isomerase) promoter (called artificial operon type) was bombarded to Yeast. Interestingly, yeast cells were progressively deformed into unusual shapes and lysed inner cytoplasm resulting in ell death after all after bombarding with expression vector (CDC and GFP). Since there is no report about more than one gene expression simultaneously in a single mitochondria, this report is very important to novel type of eukaryotic gene expression. Successful yeast cell transformation in this report implies possible eukaryotic mitochodrial transformation including plants and animals and moreover two or more gene expression which can be excellent applicable protocols to pharmaceutical field including antibody production.
Keywords
Yeast; mitochondrial; transformation;
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