• Journal of Ginseng Research
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• 제36권3호
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• pp.291-297
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• 2012

Ginsenoside (ginseng saponin), the principal component of ginseng, is responsible for the pharmacological and biological activities of ginseng. We isolated lactic acid bacteria from Kimchi using esculin agar, to produce ${\beta}$-glucosidase. We focused on the bio-transformation of ginsenoside. Phylogenetic analysis was performed by comparing the 16S rRNA sequences. We identified the strain as Lactobacillus (strain 6105). In order to determine the optimal conditions for enzyme activity, the crude enzyme was incubated with 1 mM ginsenoside Rb1 to catalyse the reaction. A carbon substrate, such as cellobiose, lactose, and sucrose, resulted in the highest yields of ${\beta}$-glucosidase activity. Biotransformations of ginsenoside Rb1 were analyzed using TLC and HPLC. Our results confirmed that the microbial enzyme of strain 6105 significantly transformed ginsenoside as follows: Rb1${\rightarrow}$gypenoside XVII, Rd${\rightarrow}$F2 into compound K. Our results indicate that this is the best possible way to obtain specific ginsenosides using microbial enzymes from 6105 culture.

• 열처리공학회지
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• 제37권2호
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• pp.79-85
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• 2024

The effects of annealing heat treatment and the addition of Cu element on the shape memory effect of the NiTi-based alloy were investigated by analyzing differential scanning calorimeter results and characterizing recovery rate through 3D scanning after Vickers hardness test. Through 3D scanning of impressions after Vickers hardness test, the strain recovery rates for specimens without annealing treatment and annealed specimens at 400, 450, and 500℃ were measured as 45.96%, 46.76%, 52.37%, and 43.57%, respectively. This is because as the annealing temperature increases, both B19' and NiTi₂ phases, which can impede martensitic transformation, are incorporated within the NiTi matrix. Particularly, additional phase transformation from R-phase to B19' observed in specimens annealed at 400 and 450℃ significantly contributes to the improvement in strain recovery rates. Additionally, the results regarding the Cu element content indicate that when the total content of Ni and Cu is below 49.6 at.%, the precipitation of fine B19' and NiTi₂ phases within the matrix can greatly influence the transformation enthalpy and temperature range, resulting in relatively lower strain recovery rates in NiTi alloys with a small amount of Cu element produced in this study.

• Mycobiology
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• 제31권1호
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• pp.42-45
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• 2003

For transformation of Pleurotus ostreatus, two novel vectors, pPhKM1 and pPhKM2, were constructed, using the regulatory sequences of the P. sajor-caju $\beta$-tubulin gene(TUB1) and the ble gene encoding phleomycin binding protein. pPhKM1 contains ble fused to the TUB1 promoter and the Schizophyllum commune GPD terminator. pPhKM2 contains ble fused to the promoter and terminator regions of P. sajor-caju TUB1. To confirm phleomycin-resistance activity, each vector was cotrans-formed with pTRura3-2 into the P. ostreatus homokaryotic $ura^-$ strain. The transforming DNA was stably integrated into the genomic DNA. Subsequently, phleomycin resistance was conferred on wild-type dikaryotic P. ostreatus by transformation with pPhKM1 or pPhKM2. This transformation system generated stable phleomycin-resistant transformants.

• 한국세라믹학회지
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• 제27권6호
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• pp.735-740
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• 1990

The phase transformation of Y-TZP by low temperature aging treatments and its related behaviors of crack formation were investigated. The kinetics of phase transformation was greatly dependent on the amounts of Y2O3, grian size and microstructures of sintered body. The phase transformation happened to start at specimen surface and near the pore in the first place, where the change of strain energy during the phase transformation was small and the water vapor that accelerated phase transformation easily diffused.

• 한국분말재료학회지
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• 제8권1호
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• pp.42-49
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• 2001

Ti-50Ni(at%) and Ti-40Ni-10Cu(at%) alloy powders have been fabricated by ball milling method, and their microstructure and phase transformation behavior were investigated by means of scanning electron microscopy/energy dispersive spectrometry, differential scanning calorimetry (DSC), X-ray diffractions and transmission electron microscopy. In order to investigate the effect of ball milling conditions on transformation behavior, ball milling speed and time were varied. Ti-50Ni alloy powders fabricated with the milling speed more than 250 rpm were amorphous, while those done with the milling speed of 100rpm were crystalline. In contrast to Ti-50Ni alloy powders, Ti-40Ni-10Cu alloy powders were crystalline, irrespective of ball milling conditions. DSC peaks corresponding to martensitic transformation were almost discernable in alloy powders fabricated with the milling speed more than 250 rpm, while those were seen clearly in alloy powders fabricated with the milling speed of 100 rpm. This was attributed to the fact that a strain energy introduced during ball milling suppressed martensitic transformation.

• 대한기계학회논문집A
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• 제25권3호
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• pp.390-398
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• 2001

Welding process generates distortion and residual stress in the weldment due to rapid heating and cooling. Welding distortion and residual stress in the welded structure result in many troubles such as dimensional inaccuracies in assembling and safety problem during service. The accurate prediction of welding residual stress is thus very important to improve the quality of weldment and find the way to reduce itself. This paper suggests new analysis method to predict welding residual stress by considering solid phase transformation during welding process. Using the method, analysis is performed for medium and low carbon steel. The analysis result for medium carbon steel reveals that case considering phase transformation has compressive residual stress in contrast with the case neglecting phase transformation because of martensite formation. However, for the case of low carbon steel, residual stress shows little difference between the case considering phase transformation and the other case, because it has small transformation strain and recovers rapidly stress after phase transformation.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권3호
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• pp.217-222
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• 2004

As Agrobacterium tumefaciens, which has long been used to transform plants, is known to transfer T-DNA to budding yeast, Saccharomyces cerevisiae, a variety of fungi were subjected to the A. tumefaciens-mediated transformation to improve their transformation frequency and feasibility. The A. tumefaciens-mediated transformation of chestnut blight fungus, Cryphonectria parasitica, is performed in this study as the first example of transformation of a hardwood fungal pathogen. The transfer of the binary vector pBIN9-Hg, containing the bacterial hygromycin B phosphotransferase gene under the control of the Aspergillus nidulans trpC promoter and terminator, as a selectable marker, led to the selection of more than 1,000 stable, hygromycin B-resistant transformants per 1${\times}$10$\^$6/ conidia of C. parasitica. The putative transformants appeared to be mitotically stable. The transformation efficiency appears to depend on the bacterial strain, age of the bacteria cell culture and ratio of fungal spores to bacterial cells. PCR and Southern blot analysis indicated that the marker gene was inserted at different chromosomal sites. Moreover, three transformants out of ten showed more than two hybridizing bands, suggesting more than two copies of the inserted marker gene are not uncommon.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2007년도 춘계학술대회 논문집
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• pp.279-280
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• 2007

In this study, it is investigated that the effect of material properties such as various temperature, forming speed and strain rates on formability and forming limits of Mg alloy sheet in square cup deep drawing. Since the sheet metal forming of Mg alloy is perform at elevated temperature, the effect of strain rates related with the forming temperature and forming speed is very important factor for formability and forming limits. Therefore, the investigation for process variables is necessary to improve formability and forming limits. Also, the effects of strain rate and thickness transformation were studied by the experimental and FE analysis using the square cup deep drawing. The temperature, forming speed, and strain rates were investigated. Forming of Mg alloy takes consider into temperature, proper forming speed and strain-rate the formed parts were good without defects fur forming limits.

• 대한조선학회논문집
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• 제39권1호
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• pp.82-89
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• 2002

선상가열에 의한 판 변형 예측은 고유변형도법에 의해 효율적으로 예측할 수 있다. 종래의 용접에서의 고유변형도 결정 방법은 용접 실험을 통하여 온도분포와 강의 상변태 영역(Ac3)을 시편을 절단하여 계측하고 이를 고유변형도 영역으로 간주하는 것이었다. 선상가열의 현상은 용접과 유사하므로, 용접과 같은 조건 하에서 얻어진 결과를 그대로 선상가열 해석에 이용하여 왔으나 이 결과는 가열 패턴이나 판 두께에 제한을 가지고 있다. 또한 현장에서는 선상가열 후 수냉 처리하는데 그 과정에서 강이 원래의 상으로 돌아가지 않고 마르텐사이트가 되면서 전단 소성 변화를 일으킨다는 점에 착안하여, 본 연구에서는 종래에 시편의 온도계측과 상변태 영역을 직접 계측하는 파괴검사법을 FEM을 이용한 이론해석으로 대체하였다. 즉 임의의 적절한 열속 모델에 대한 온도 분포를 얻고, 조직변화에 따라 추가적으로 발생하는 소성 영역을 고려하기 위하여 공석 온도 영역까지 포함시켜 온도계측과 파괴실험 없이 순수한 이론만으로 고유변형도 영역을 결정하는 새로운 방법을 제안하였다. 이 방법으로 결정한 영역을 이용하여 판의 변형을 예측하고 실험 결과와 비교하여 잘 일치함으로써 본 논문에서 제안한 방법의 유효성을 입증하였다.

• Journal of Microbiology and Biotechnology
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• 제9권5호
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• pp.687-690
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• 1999

Bacillus stearothermophilus No. 236 isolated from the soil is a strong xylan degrader producing all the xylanolytic enzymes. However, the strain was discovered to be highly intractable to its transformation. In the present study, we have developed a reliable method for transformation of B. stearothermophilus No. 236 by a systematic examination of several factors which might have an influence on the efficiency of electrotransformation. Notably, we found that the most critical factor influencing the transformation efficiency (TE) was the incubation temperature after pulsing, with its optimum incubation of $37^{\circ}C.\; At\; 50^{\circ}C$, the optimum growth temperature of the B. stearothermophilus strain, the transformants could not be obtained at a recognizable level. The combination of field strength of 7.5 kV/cm along with pulse duration of 10 msec (resistance of $400{\Omega}\; and\; capacitance\; of\; 25{\mu}F$) was shown to be the best electrical parameters at the incubation temperature of $37^{\circ}$. A higher TE was obtained when the cells were harvested at an early-exponential phase. Twenty percent of PEG-8000 in a suspension buffer and an addition of 0.1% glycine in the growth medium resulted in about 4-fold and 3-fold increases in TE, respectively. We also found that the plasmid DNA which had been cycled through the host B. stearothermophilus cells enhanced TE by one order of magnitude higher. Under the presently described conditions, $2.5{\times}10^{5} transformants per ${\mu}g$ DNA was attained.